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Analysis of candidate transmembrane adaptor proteins
Šulcová, Jitka ; Černý, Jan (referee) ; Drbal, Karel (advisor)
Transmembrane adaptor proteins (TRAPs) function as a scaffolding anchor for numerous signaling molecules and facilitate formation of large signaling complexes near the plasma membrane. They regulate spatio-temporal organization of signaling events in order to transduce fast and effective signal from surface receptors into the cell nucleus. The importance of described TRAPs in the development and proper functioning of immune cells in relation to their localization within lipid rafts is still under debate. The simple reason is the lack of a profound phenotype in knock-out mice. The described phenotypes observed in animals deficient in ITAM-containing adaptors of immunoreceptors as well as lipid raft adaptor Lat all lead to the block in the T-cell development and impaired TCR signaling. The subject of my diploma thesis is to perform bioinformatic search and filter candidate TRAP genes according to predicted and published data. Selected TRAP candidates were subjected to functional characterization. I checked for their localization to the plasma membrane and correlate their ectopic overexpression with the expression of CD69 surface Tcell activation marker. From 14 candidate TRAPs, only a single Pdzk1ip1 protein is probably localized to the plasma membrane but its expression does not influence CD69...
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Changes in the composition and localization of gangliosides in cholestasis associated with other markers of pathological processes in hepatocytes.
Petr, Tomáš ; Muchová, Lucie (advisor) ; Mičuda, Stanislav (referee) ; Befekadu, Asfaw (referee)
This thesis is focused on the study of glycosphingolipids in the rat liver in different types of cholestasis and the effect of oxidative stress on changes in the composition and localization of gangliosides. First, it was necessary to optimize the immunochemical detection of glycosphingolipids. GM1 ganglioside was selected as a representative of a large glycolipid family. We found that minimum water content in the fixing solution was a key condition for fixation of histological sections. Optimized method of GM1 detection was subsequently used in in vivo experiments. We have demonstrated that estrogen-induced cholestasis characterized by high concentrations of bile acids and increased oxidative stress caused changes in the synthesis and distribution of liver gangliosides. HMOX induction is associated with a reduction in oxidative stress level and accompanied by normalization in GSL content. In experiments with obstructive cholestasis, we found that changes in the distribution and synthesis of gangliosides were not strictly specific to a particular type of cholestasis. We assume that it represents a general mechanism of hepatoprotection. We also confirmed the important role of bilirubin, product of HMOX reaction, in protection of hepatocytes against oxidative damage caused by high concentrations of...
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The Role of the Liquefied Natural Gas in the World Economy
Hričková, Michaela ; Kašpar, Václav (advisor) ; Vošta, Milan (referee)
The Bachelor thesis' focus is on the role and importance of the liquefied natural gas as a tradable commodity and a way of transporting natural gas. The first part of the thesis contains an analysis of the current state of the world natural gas market including factors influencing said market and the pattern of localisation of natural gas fields in the world. The second part deals with the specific features of LNG and its production and transportation issues. The last part analyses the position of LNG in the world energy mix and the localisation of exporting and importing LNG facilities around the world.
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Modulace exprese alfa-synukleinu pomocí působení 5-S-cysteinyldopaminu na buňky lidského neuroblastonu SH-SY5Y: možná úloha katecholthioetherů při neurodegeneraci
Hrabáková, Rita ; Štaud, František (advisor) ; Mladěnka, Přemysl (referee)
-Synuclein is a presynaptic protein which has been demonstrated to be involved in PD etiopathogenesis.It can regulate DA homeostasis by inhibition of TH activity, by regulation of the DAT activity and finally by potential role in vesicular storage. - Synuclein is a natively unfolded protein, which can undergo overexpression and aggregation due to toxic insults or oxidative stress. The aggregation of α-synuclein leads to a loss of function, which in PD neurons may determine a dysregulation of the DA pathways with subsequent excess of cytosolic DA, that can enhance the neurotoxic effect of α-synuclein aggregates. In recent years, a catecholthioether metabolite of DA, 5-S-cysteinyl-dopamine, has been identified in certain dopaminergic regions of the brain, notably the Substantia nigra. Cys-DA seems to have a possible significance as an index of oxidative stress in aging and in neurodegenerative processes and it was recently hypothesized that this substance or its metabolites may be the endogenous neurotoxins responsible for neurodegeneration in PD. Hence, the aim of this experimental work was to determine whether Cys-DA is able to cause overexpression of -synuclein both at transcriptional and translational levels in a cellular model of PD, the human neuroblastoma dopaminergic cell line SH-SY5Y....
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Images of Animals and Their Roles in Poems of Emily Dickinson
HRONOVÁ, Andrea
The thesis focuses on images of animals, their roles and meanings in the poems of Emily Dickinson. Starting with Dickinson and her relationship to animals, I will move to general characteristic of images and then to images of animals, which are at first described generally, then I will aim on the particular groups of animals. The ideas said above are demonstrated on the interpretations of poems, which are included in the thesis.
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Effectiveness and limits of protection of civilians in the context of humanitarian crises: Haiti Case Study
Svobodová, Valérie ; Peterková, Jana (advisor) ; Knotková, Vladimíra (referee)
The main intention of this paper is to provide a better understanding of the mechanisms of the protection of civilians in the context of humanitarian crises. The first part provides theoretical definition of the protection of civilians and its legal underpinnings. The roles of individual actors such as the state and its agencies, UN peacekeeping missions, cluster system, non govermental organisations and civil society itself are being defined. The paper futher focuses on the prevention in humanitarian crises, especially on the principles of peacekeeping, human security and gender. The second part consists of a case study of Haiti in the context of the 2010 devastating earthquake and the ensuing humanitarian crisis. First it discusses the impact of the earthquake on the infrastructure, environment, government, economy and education as well as its consequences for the civilians themselves. The impact of the earthquake is compared with the priorities of the state, as seen after the earthquake. Response to the earthquake is cathegorized as national and international, each of which has its specific characteristics and is based on different prior and post disaster conditions. Specific groups of civilians - minors and civilians affected by gender violence- are focused on .The protection of civilians in the specific areas is also addressed - namely duringthe cyclone Thomas, in the IDP camps and in the area struck by the cholera epidemics. The work culminates in the research which consists of three parts - a questionnaire for NGOs, civilians and government officials.
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Biologie rozmnožování u raků
NIKSIRAT HASHJIN, Hamid
The ultrastructure of spermatozoa from six crayfish species were described. The acrosome complex and nucleus are located at the anterior and posterior of the spermatozoon, respectively. The acrosome complex organelle is divided into two main parts: the main body of the acrosome that is a dense inverted cup-shaped structure and organized into three layers of differing electron densities and extended parallel filaments, and the sub-acrosome zone occupying the central part of the acrosome complex, which is divided into two electron dense areas. The spermatozoon of Orconectes limosus is described for the first time. In addition, an acrosome spike in the spermatozoon of Procambarus clarkii is described. Despite a well conserved general structure and similarity of pattern among these spermatozoa, differences in the dimensions of the acrosome within the studied species may be useful to help distinguish the different crayfish. The ultrastructure of the spermatozoon and spermatophore wall of the narrow clawed crayfish Astacus leptodactylus during three stages including freshly ejaculated, post-mating, and after release of the spermatozoon were described and compared. The crayfish spermatophore consists of a sperm mass enveloped by a three layered spermatophore wall. After mating, the thickness of the outer layer of the spermatophore is increased. The matrix in the middle layer of the spermatophore becomes reticulated, and granules inside this layer release their contents. Fibers in the inner layer degrade to small particles. The spermatozoon capsule swells and space between the capsule and the spermatozoon appears. The area of the plasma membrane is increased by wrinkling of the surface and alteration from a single to a multilayered structure at the anterior part of the acrosome. The density of the subacrosome zone increases in the vicinity of the main body of the acrosome. With the onset of fertilization, the layers of the spermatophore are dissolved by female glair glands secretions. The capsule, plasma membrane, and membranous lamellae are eliminated, and bundles of filaments are released. The subacrosome zone loses electron density and retracts. The electron-dense material of the innermost layer of the acrosome is discharged and, together with filaments, forms a filament/droplet structure at the anterior part of the spermatozoon. The most important change is observed in the subacrosome zone, which may play a key role in the fertilization. Also, morphological changes of the spermatozoon after release from the capsule, especially formation of the filament/droplet structure, may contribute to the mechanism of egg-spermatozoon binding in the crayfish.
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Elements of Deliberative Democracy in the Czech Republic: A Case Study of Participatory Budgeting in Municipal District of Prague 10
Šmerdová, Vladimíra ; Masopustová, Ilona (advisor) ; Jüptner, Petr (referee)
This thesis lies in an analysis of a participative budget realization at the Prague 10 municipal district in a context of deliberative democracy. This is a case study stemming from a qualitative research based on an analysis of key documents and semi- structured interviews. Deliberative and participatory democracy - whose features are the crucial elements in a participatory budgeting process - serve as a theoretical basis of this thesis. In the empirical part the thesis focuses on causes of the participatory budgeting implementation and the laid down methodology approved by the Prague 10 municipal assembly in June 2015. Neither rules for proposal submissions nor the time schedule of the process are omitted. The thesis deals with different phases and the future outlook as well. An integral part of the thesis is the analysis of the actors who played key roles in the process. Apart from the case study the thesis maps the use of the participatory budgeting both abroad and in other Czech towns and cities.
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Activities fostering cooperative behaviour in pre-school children
BAKEŠOVÁ, Ivana
The diploma thesis presents a differentiation and description of various forms of interpersonal interaction with emphasis on cooperation and cooperative behaviour from the perspective of sociology, social psychology and psychology of personality. As the research is focused on preschool children, the characteristic traits of this age are specified, specific methods and forms suitable for the development of cooperative behaviour are described as well as the role of the teacher in this process. Moreover, the aim of fostering cooperative behaviour is linked to the educa?tional purpose outlined in FEP PE and Learning: The Treasure Within (four pillars of education). Furthermore, variables related to individual dispositions in respect to cooperative behaviour are briefly analysed. In the research section of the thesis, activities aiming at fostering cooperative behavior in preschool children are presented and evaluated based on their testing in a pre-school: whether/how they really worked in respect to eliciting cooperative behaviour in children.
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Za rámec běžného plánování podníkání: budování internetového startupu v rychlé ekonomice
Šimek, Vojtěch ; Krause, Josef (advisor) ; Vaněček, Michal (referee)
First part of the thesis compares available approaches on business planning, namely the traditional and newly emerged and proposes a Business Planning Approach Selection Framework that should help (newbie) entrepreneurs with orientation in the chaotic business planning world and choosing an approach suiting their specific needs. Conclusion of the theoretical part is that both the traditional and modern business plans have their role in todays business environment. While the old are great for external presentation and funds securing, the new enable to create more complex process for planning successful business from the original idea to and beyond their launch, building on customer feedback and gradual iterations. The practical part then builds on the theoretical research and aims to check feasibility of an actual business project, a multisided platform mediating sales of automotive financial products. It describes the model refinement and product creation process as well as eventually proves viability and feasibility of the whole business.
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