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Biodegradation of environmental pollutants - Structural characterization of a new type of haloalkane DbeA
PAPEŽ, Martin
The aim of this work is the crystallization of model protein Xylanase and a new haloalkane dehalogenase DbeA and description of DbeA structure using modeling programs. For this purpose, the crystallization methods based on sitting and hanging drop vapor diffusion, crystallization under oil and crystallization in capillaries were applied. The structure was solved using programs WinCoot and VMD. The results will be used for further research of structure-functional relationships of haloalkane dehalogenases.
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Biodegradation of environmental pollutans - Crystallization haloalkan dehalogenase DpcA of Psychrobacter cryohalolentis K5
JUNG, Jakub
Knowledge of protein structures is necessary to clarify their structure-function relationships. Haloalkane dehalogenases are microbial enzymes that catalyze the hydrolysis of carbon-halogen in the halogenated hydrocarbons. Based on their properties these enzymes are able to degrade halogenated compounds. As the environment is burdened by a lot of different pollutants, biodegradation is a necessary step to improve the quality of environment. Haloalkane dehalogenases can be used as biosensors since they can detect contamination by halogenated compounds. This thesis is focused on crystallization of the model protein lysozyme and haloalkane dehalogenase DpcA isolated from the organism Psychrobacter cryohalolentis K5. The aim of this work was to prepare crystals suitable for X-ray diffraction analysis. After verification of protein?s purity, both proteins were crystallized by the use of basic and advanced crystallization methods. Single crystals of suitable size and quality for X-ray diffraction analysis were obtained and diffraction data were used to solve the 3D atomic structure of the DpcA. Since haloalkane dehalogenases have a low activity for halogenated substrates, the main aim of protein engineering is to increase their activity so thus further research is focused on modification of substrate specificity.
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The effect of atenolol on a fish organism
BOŘÍK, Adam
Pharmaceuticals and their metabolites are emerging pollutants of aquatic environment associated with our modern society. The aim of this bachelor's thesis was to get information about potential hazard levels of atenolol occurring in surface waters and its effects on juveniles of rainbow trout (Oncorhynchus mikyss) as a model organism. The effect of environmentally relevnt concentrations of atenolol was assased in vivo by using biochemical markers. Activity of enzymatic reactions EROD (CYP 1A), MROD (CYP 1A2) and PROD (CYP 2B) were tested as possible biomarkers. The experimental work aimed to verify wheter selected cytochromes P450 are suitable to evaluate the effects of fish exposure in natural conditions. The results of present experiment did not show a relationship between the enzymatic activity and exposure to beta-blocker atenolol. This fact implies that the enzimatic reaction EROD, MROD and PROD cannot be useful as biomarkers reflecting a metabolic response of fish after exposure to this xenobiotic.
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Environmental control system of aircraft cabin
Fojtlín, Miloš ; Hejčík, Jiří (referee) ; Fišer, Jan (advisor)
In this research work available information about air-conditioning systems of actual airliners are processed. These systems solve in complexity aircraft cabin microclimate modification into form comfortable and acceptable for the human body (ECS – enviromental control system). The work also covers ECS subsystems description. It explains their function, thermalmechanical principle, different possibilities of their realization and overall ECS function. Examples of ECS negative impact on other aircraft systems are also mentioned. Afterwards, real schemes of ECS built on board are sketched. The paper also presents reasons of ECS introduction, concrete requirements to cabin microclimate out of consideration to safe stay of human bean on board in different flight procedures. Simultaneously technical obstacles and limitations of ECS are shown. In conclusion need of correct ECS function is highlighted.
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Influence of environmental variables on the shape of ultraviolet pattern in Gonepteryx rhamni
Pecháček, Pavel ; Kleisner, Karel (advisor) ; Hořák, David (referee)
Many species are sensitive to a light in ultraviolet spectrum. Some species have surface patterns that reflect ultraviolet light. These markings have been observed in many animal taxa; butterflies (Lepidoptera) are no exception. UV-reflectance in butterflies has been primarily connected to sexual selection and in this respect it has been a subject of many studies. In my work I propose an alternative view to this phenomenon. The aim of my work is to reveal how a particular environmental factors influence the morphospace of UV- reflectant patterns and wing shape of the Gonepteryx rhamni (Pieridae). The effect of various environmental factors (latitude, longitude, altitude, mean annual temperature, mean annual precipitation, normalized difference vegetation index - NDVI or net primary productivity - NPP) on wing morphospace was tested using the methods of Geometric morphometrics. I have also studied shape variability among the males and females, specimens from different locations and differences in morphospace of several G. rhamni subspecies. The dataset used in this analysis includes 118 males and 67 females from the Palearctic ecozone. The effect of almost all environmental (except to NDVI and NPP) predictors on shape of the UV-pattern and wing margin was significant in the case of males. In the...
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Current environmental threats and their impact in the landscape
Zapletalová, Jana ; Kirchner, Karel
Workshop Current environmental threats and their impact on the landscape was organized in the framework of the Strategy AV21 of the Academy of Sciences, the program Natural threats.\nThe papers were oriented both on natural threats (geological and geomorphological threats - slope deformation, erosion, hydrometeorological extremes floods, droughs, biological invasions) and their evaluation and prediction, and social and legal implications of natural and human activities caused by threats and their impacts on the landscape (e. g. changes in the urban environment, anthropogenic impacts in the mining areas). Important topics were focused on the assessment of natural and environmental threats and their solving in selected emergency and crisis situations using geoinformation technologies. \n
Fulltext: content.csg -  PDF
Plný tet: UGN_0465323 - PDF
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The environmental aspects in the hotel industry
Nováková, Eliška ; Jenčková, Jiřina (advisor) ; Valentová, Jana (referee)
The Dissertation Thesis conducts a survey of the level of environmental manners in accommodation services in selected states. Through structured interviews, it maps an interest in keeping environmentally friendly policy in the Czech hotel industry. The research has been carried out in eco accommodation facilities in the Czech Republic. For comparison, there have been used the findings obtained from hotel type accommodation facilities with the same specialization in Austria. Based on the revealed facts, the Thesis proposes recommendations regarding the improvement of environmental culture in the Czech accommodation services. The introductory part presents the theoretical definition of environmental aspects, including the definition of eco accommodation facilities and eco-labels used by environmentally friendly accommodation facilities. Further, there are listed various tools to reduce impact on the environment, and to eliminate negative influences, and also guidance for environmental education. The conclusion part presents the results of the survey, stemming from the theoretical part, and it also suggests recommendations to improve current situation in the hotel industry.
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Analysis of Tutorials CEGV Cassiopeia in Terms of Primary Prevention
KURZ, Petr
Analysis of teaching programs of CEGV Cassiopeia from the view of primary prevention. The paper deals with the topic of primary prevention in relation to teaching programs carried out by CEGV Cassiopeia. The Center of Ecologic and Global Education Cassiopeia (CEGV Cassiopeia) was established in 1995. At present its primary occupation is education, particularly education specialized in environmental, multicultural and personal-social topics. The center co-operates with nursery, primary, high schools, specialized schools and universities. It is the object of this paper to evaluate individual teaching programs on the basis of standards of "Competence certification of providers of programs of primary prevention of high-risk behavior at schools". The theoretical part deals with basic terms in primary prevention in relation to education and compiles the historical development as well as environmental education. The practical part provides an analysis of teaching programs on the basis of certification standards. The analysis gives an overview of teaching programs appropriate for the certification process. There are also results of interviews with CEGV Cassiopeia lectors and teachers relating to helpfulness of environmental teaching programs from the perspective of primary prevention.
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Environmental Topics in Teaching Music
Žídková, Johana ; Kodejška, Miloš (referee) ; Váňová, Hana (advisor)
The content of the diploma thesis named Environmental Topics in Teaching Music outlines possibilities of how to include environmental features in the school subject of Music. The necessity of dealing with the topic of ecology has arisen from the implementation of Framework Education Programmes for elementary and secondary education in the Czech Republic in 2007. These include, apart from the traditional educational areas, new cross-curricular subjects. One of them is environmental education. Unfortunately, there are not enough materials on how to implement crosscurricular subjects into music lessons. Therefore, the aim of this work is to provide an overall view of the field for those who would like to start teaching environmental education through music, to give examples of a successful application abroad and finally, to point out possibilities for teaching on the domestic front. There are two levels on which the implementations take place: on the level of organizational forms (in lessons, projects, etc.) and on the content level (through the choice of topics or cross-subject connection). These two elements are eventually connected by the synthesis of environmental and musical methodology mostly based on multiple potential of music itself. Music becomes both: the means and the aim.
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Návrh pro zvýšení rekreační atraktivity území se zřetelem na realizaci agroturistiky
Šeffer, David
The topic of this thesis is " The proposal to increase the attractiveness of recreational area with regard to the implementation of agri-tourism". The introductory part deals with typology of holidays, rural tourism and environmental agriculture. Adding to it, the work describes Moravskokrumlovsko micro-region relating to rural tourism. Another part of the work deals with design of Dobelice goat farm and reconstruction of involved buildings. Based on the farm design a business plan was prepared and at the same time suggestions for increase in rural tourism in the village were raised.
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