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Lyme borreliosis
Kohoutová, Lucie ; Jílek, Petr (advisor) ; Křivčíková, Lucie (referee)
Author: Lucie Kohoutova Title: Lyme borreliosis (Diploma thesis) University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the presence of Lyme disease in the area of Havlickuv Brod based on laboratory diagnostics of specific or non- specific antibodies in the blood serum and in the liquor. The aim was to define typical laboratory signs of illness and recommend racional methods in diagnosis of Lyme disease. Methods: We used the data from Havlickuv Brod hospital laboratory of immunology. Patients were dividend into the groups according to the results of basic tests (if there were immunoglobulines type M and type G in serum or liquor), sex, age, the day of blood collection, code of diagnosis, patient's doctor and the type of running tests (ELISA, westernblot). These groups were compared. Patients with at least one positive result in basic tests and with any of additional test at the same time were divided in compliance with relations amongst these types of tests. We tried to determine appropriate interval between taking samples during monitoring the dynamics of the disease by contribution- based method. Results: More threatned by Lyme borreliosis are older women and people over the age of fifty- five. The highest number of new...
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The Role of a Nurse in Care of a Patient with Mononucleosis
LAŽANSKÁ, Soňa
Theoretical background Infectious mononucleosis belongs to relatively widespread viral infectious diseases in the Czech Republic. Epstein-Baar virus (EBV) participates in occurrence of the infection by 80 %, infectious mononucleosis may more rarely be caused by cytomegalovirus. Each individual factually suffers from infection caused by EBV during his/her life, however its course is unapparent in most cases. An affected individual or an individual not affected, who is a virus carrier may be considered the source of the infection. Infectious mononucleosis usually affects children and teenagers, in older individuals the infection is quite rare. The disease might start rapidly or gradually with prodromes like headache, sore throat, nausea, fatigue and perspiration. Infectious mononucleosis is often confused with other diseases like e.g. tonsillitis, or more serious progress of flue, particularly for the symptoms accompanying the disease. Fever, lymphadenopathy and hepatosplenomegaly are the most frequent symptoms. The disease may also cause numerous unpleasant complications, particularly hematologic, neurologic, cardio-pulmonary and autoimmunity complications. A nurse plays a very substantial role in client care. It is very important for a nurse to have sufficient knowledge and to know the right procedure of all interventions he/she meets within diagnostics and treatment of infectious mononucleosis. These are particularly biological material collection, per os administration or intravenous administration of medicines. A nurse should also be familiar with the Priessnitz compress application technique. In non-self-sufficient clients a nurse is also responsible for client's hygiene. A nurse has an important role in client education providing information on dietetic measures. A nurse has to lead a client to adherence to the prescribed diet and body calm. Everything depends on client's current condition. A client should also avoid mental strain during convalescence. We should note that not all forms of infectious mononucleosis require hospitalization. The aim of the thesis The aim of the thesis was to find out the role of nurses in the care of patients with infectious mononucleosis and also to find how satisfied patients with the nursing care and education are after going through mononucleosis. This was why the following research questions were chosen: What is the role of a nurse in care of a patient with mononucleosis? How satisfied are patients with the nursing care after going through mononucleosis? How satisfied are patients with education in diet regime adherence after going through mononucleosis? Applied Methods Qualitative research was chosen for finding out the required information. The information was gained by means of deep interviews with nurses working at the infectious department of České Budějovice Hospital and with clients after going through infectious mononucleosis. All the interviews were recorded on a voice recorder and then transcribed. The transcribed interviews were subject to detailed analysis by the paper and pencil method with open coding. Results Six categories were set upon analysis of the interviews with nurses: Awareness of the infectious mononucleosis disease among nurses, Nursing care of a client with infectious mononucleosis, Regime measures, Information on dietetic measures, Recommendations after infectious mononucleosis and Education of a client with infectious mononucleosis. Five categories were set upon detailed analysis of the interviews with the clients: Recommended treatment, Nursing care, Recommended measures, Methods of information communication and Information sources for clients. Subcategories, in which the key data are coded, are assigned to the individual categories.
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Jean Ray, a Belgian author of fantasy short stories
Tauchmanová, Jana ; Jamek, Václav (advisor) ; Pohorský, Aleš (referee)
Raymundus Joannes Maria De Kremer, best-known under the pseudonym of Jean Ray, was one of the most important Belgian authors of the first half of the twentieth century. His contribution to literature is immense, both in its volume and in its diversity, stretching well over sixty years of uninterrupted writing. While he wrote mostly short stories of the fantastic genre and adventure novels, he also published detective stories under the pseudonym of John Flanders. A major part of his work was published in newspapers and magazines. Jean Ray was bilingual, which allowed him to write both in French and Flemish, dividing his attention equally between the two. The majority of his writings was published either anonymously or under various pseudonyms. The aim of our thesis is to provide a monograph overview of the said author. The first part situates his work in the context of Belgian literature written in French and the fantastique genre in general. The second part is devoted to his life, with special focus on his literary career. The third part contains analyses of selected parts of his extensive oeuvre. Briefly, we look at the Harry Dickson detective stories. However, the core of our analysis are his fantastic writings, i.e. selected collections of his fantastic short stories and the only fantastic novel...
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The influence of nutrient loading, meteorological and hydrological conditions and operating manipulations on phytoplankton in the water suply reservoir Římov.
Hejzlar, Josef ; Jarošík, Jiří ; Nedoma, Jiří ; Seďa, Jaromír ; Znachor, Petr
Analysis of data collected during long-term and complex limnologic monitoring of the Římov reservoir in the period 1983 – 2015, which depict the development of physico-chemical conditions, hydrology and hydrodynamics of the reservoir with links to biological data on phytoplankton, zooplankton etc. showed that the concentration and species composition of phytoplankton depend on the supply and availability of nutrients, but are also influenced by climatic and hydrological conditions and water management operation of the reservoir.\n
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Individual human odor as a forensic trail in criminal proceeding made by contact or contactless transmission and its resistance to physical agents
Santariová, Milena ; Bartoš, Luděk (advisor) ; Chmelíková, Eva (referee)
The dissertation thesis consists of four papers that have been published in scientific journals.
Study n. 1
The need to recover evidence from water is quite common in criminal investigation. The article deals with the possibility to collect human scent from such objects and with the ability of specially trained dogs to match such scent samples with scent samples collected from detained suspects. During an experiment, designed as a blind one, it has been proved, that human scent can survive on submerged objects and can be later used for scent identification.
Study n. 2
To collect odors the Czech Republic Police use special fabric sorbents manufactured under the registered mark ARATEX. Before use the fabric sorbent is treated by water vapor sterilization. After the scent identification the sorbent is destroyed. The goal of the study was to verify if the vapor sterilization is effective enough to remove human scent that has already penetrated into the sorbent structure or in other words if the sorbent can be exposed to vapor sterilization and then used again. Specially trained dogs were used to match starting odors with target odors in the line-ups. The results showed that dogs are able to correctly match human odors even after they have being exposed to vapor sterilization.
Study n. 3
The purpose of the study was to determine the temperature at which the human scent is degraded so that a dog would not be able to identify it. In contrast to expectations, eight dogs used in the experiment almost flawlessly identified human scents from five scent donors exposed to temperatures of 100°C, 200°C, 300°C, 400°C, 500°C, 600°C, 700°C, and 800°C. Only two of the dogs were able to identify 5 of 15 scent samples exposed to 900°C. No dog identified a scent exposed to 1000°C. Our study verified heat survivability of human scent far beyond existing expectations.
Study n. 4
We tested the hypothesis that if odor fallout (the release of a human odor onto an untouched object) in human subjects exists, then holding a hand above an absorbent will produce a detectable scent which will be subsequently matched in a detection test by trained dogs. Scents were collected from seven males to sterile cotton absorbent squares. The left hand was used to get the control scent and the right hand served as the target scent. Each experimental subject was sitting and his left hand was laid down on a cotton square for 3 min. The right hand was held 5 cm above another cotton square for 3 min. The scent identification was done by two specially trained police German shepherds. Both dogs performed 14 line-ups and correctly matched the collected scents of all test subjects. The results suggest the existence of human odor fallout, whereby a human scent trace is left by humans even if they do not touch an object.
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Total contents and speciation of arsenic and selenium in plants growing in soils with different physico-chemical properties
Tremlová, Jana ; Száková, Jiřina (advisor) ; Vymazal, Jan (referee)
This dissertation thesis deals with uptake, accumulation and transformation of arsenic and selenium in plants growing on soils with different chemico-physical soil properties. The contents of arsenic were investigated in 99 wild plant species, coming from 27 different families and 6 different vegetables growing in arsenic contaminated soils. The results suggest that there are species-specific mechanisms which protects certain plants from the excessive uptake of arsenic from the soil. On the other hand, there are plants using a strategy of accumulation, which theoretically may increase the risk of arsenic entry into the food chain, whether through grazing cattle and wildlife or through consumption of vegetables growing on arsenic contaminated soils. The most prevalent arsenic compounds are AsV and AsIII. Other arsenic compounds occure as minor species. An important finding was detection of arsenobetaine in Plantago lanceolata L. and Carex praecox Schreb ..
The contents of selenium were examined in 73 species of wild plants, coming from 29 different families. The results suggest that naturally low levels of selenium in the soil of selected locations along with a low capability of selenium uptake via collected plant species lead to the low content of selenium in plant biomass which may contribute to selenium deficient throughout the food chain. Foliar application of selenate on wild plant communities and on some types of vegetables in our case Brassica oleracea var. italica can have a positive impact on increasing the selenium content in the aboveground biomass of these plants and by extension, increase the selenium content in the human diet. Dominant selenium compounds in the aboveground biomass of the analyzed plants were SeVI and selenomethionine, with variable proportion of other commonly occurring organic selenium compounds, which is mainly affected by plant species.
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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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Provision of alternative refuse collection for a real estate surrounded by foreign property
Kvapil, Radek ; Štindl, Michal (referee) ; Klika, Pavel (advisor)
The thesis deals with possible ways of drainage and wastewater disposal. The issue of wastewater discharge to the environment has been closely monitored in recent years, and there is an apparent effort to improve the current situation.The dissertation discusses and compares three selected options – access to improved sanitation, building reservoirs and domestic wastewater treatment plant.The final section is dedicated to a comparison of acquisition and operating costs over time and to subsequent evaluation of the most suitable option. For the sake of clarity and assessment of potential solutions, the locality in the administrative area Vysoké Mýto - Domoradice has been selected as a model. The local authorities have been designing a public storm sewer, and given that it is surrounded by land, this issue will need to be addressed.
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Swarm Intelligence
Winklerová, Zdenka ; Šaloun, Petr (referee) ; Škrinárová,, Jarmila (referee) ; Zbořil, František (advisor)
The intention of the dissertation is the applied research of the collective ( group ) ( swarm ) intelligence . To demonstrate the applicability of the collective intelligence, the Particle Swarm Optimization ( PSO ) algorithm has been studied in which the problem of the collective intelligence is transferred to mathematical optimization in which the particle swarm searches for a global optimum within the defined problem space, and the searching is controlled according to the pre-defined objective function which represents the solved problem. A new search strategy has been designed and experimentally tested in which the particles continuously adjust their behaviour according to the characteristics of the problem space, and it has been experimentally discovered how the impact of the objective function representing a solved problem manifests itself in the behaviour of the particles. The results of the experiments with the proposed search strategy have been compared to the results of the experiments with the reference version of the PSO algorithm. Experiments have shown that the classical reference solution, where the only condition is a stable trajectory along which the particle moves in the problem space, and where the influence of a control objective function is ultimately eliminated, may fail, and that the dynamic stability of the trajectory of the particle itself is not an indicator of the searching ability nor the convergence of the algorithm to the true global solution of the solved problem. A search strategy solution has been proposed in which the PSO algorithm regulates its stability by continuous adjustment of the particles behaviour to the characteristics of the problem space. The proposed algorithm influenced the evolution of the searching of the problem space, so that the probability of the successful problem solution increased.
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