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Experimental investigation of a control synthetic jet
Němcová, L. ; Kordík, Jozef ; Trávníček, Zdeněk ; Kopecký, V.
Synthetic jets were used in previous studies to control a continuous round air jet. This study focuses on the single synthetic jet, which is measured using Constant Temperature Anemometry (CTA) and Particle Image Velocimetry (PIV). Velocity profiles and frequency characteristics of the single synthetic jet are discussed and the velocity maps at frequencies of 30 Hz, 81 Hz and 190 Hz are presented. Finally, velocity, flow rate and momentum coefficients featured the present flow control are evaluated, and examples of actively controlled round air jet are put on. The results confirm efficiency of the present flow control method.
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Forensic investigation and economic crime
Švajnochová, Gabriela ; Müllerová, Libuše (advisor) ; Hótová, Renáta (referee)
Topic of this master thesis is forensic investigation and forms of economic crime which can be detected during the investigation. First chapter focuses on forensic investigation and its comparison with statutory audit. In the second chapter is performed analysis of forms of economic crime in the Czech Republic, Southern and Eastern Europe and globally. Knowledge from the previous chapters is applied to the real forensic investigation performed in the Czech Republic, forensic investigation of project OpenCard.
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Synura echinulata (Synurales) - silica scale plasticity in natural populations influenced by environmental factors; accompanying scaled chrysophytes
Kreidlová, Jana ; Němcová, Yvonne (advisor) ; Hodač, Ladislav (referee)
The silica scale shape variation in Synura echinulata has been investigated only in laboratory conditions, so far. The aim of this thesis was to study scale shape variation in natural populations and to determine which environmental parameters influence the scale shape. The scale shape variation was investigated using landmark-based methods of geometric morphometrics. Scale shape changes related to environmental factors were analyzed using the two-block method PLS (Partial Least-Squares analysis) and adonis (R). The scale shape was mostly influenced by locality, pH and altitude. Shape distinguished populations of S. echinulata probably exist in different localities. The more distant localities are, the less similar are scales of S. echinulata in their shape. This scale shape variation is probably genetically determined, even thought all so far sequenced populations belong to the same species. High morphological disparity which was probably caused by anthropogenic pollution, was recorded in the locality Brdy. During sampling of the natural populations of Synura echinulata in the Czech Republic, several localities representing a significant reservoirs of species diversity, were found. Therefore, the thesis was extended and biodiversity research in the Czech Republic was added. A few samples were...
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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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The role of acetylation in the RNA recognition motif of SRSF5 protein
Icha, Jaroslav ; Staněk, David (advisor) ; Šenigl, Filip (referee)
Acetylation is emerging as an important posttranslational modification, which is found in thousands of proteins in eukaryotes, as well as prokaryotes. Global proteomic studies implicated acetylation in regulation of various processes like metabolism, gene expression, cell cycle or aging to name a few. In this work I set out to investigate the role of acetylation of a splicing regulatory protein SRSF5 by creating mutations in its acetylation site. I tested the hypothesis that acetylation influences SRSF5 interaction with RNA. I expressed acetylation-mimicking (Q) or non-acetylable (R) mutant of SRSF5 in HeLa cells and measured their interaction with RNA by RNA immunoprecipitation or in vitro by fluorescence anisotropy. Both approaches agreed that mutants interact with RNA less than the wild type protein and Q mutant bound RNA weaker than R mutant. I did not detect further difference in localization or dynamics among the proteins in vivo, which suggests that difference caused by weakened interaction of mutants with RNA was outweighed by other factors influencing SRSF5 behaviour, probably protein-protein interactions. I also found out that mutant SRSF5 proteins do not have a dominant effect on splicing of fibronectin alternative EDB exon. The data obtained give an indirect evidence for the hypothesis that...
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HYDROGEN ABSORPTION IN A-Co30Fe55B15
Čermák, Jiří ; Král, Lubomír ; Roupcová, Pavla
Hydrogen solved in amorphous alloys (AAs) influences their magnetic characteristics. AAs are also perspective\nas additives that can improve hydrogen storage kinetic in certain types of ball-milled hydrogen storage\nmaterials (HSMs). Therefore, knowledge of hydrogen solubility and hydrogen sorption kinetics in AAs are of a\ngreat importance for aimed design both AAs with optimal magnetic parameters and HSMs with desired sorption\ncharacteristics. In the present paper, amorphous alloy Co30Fe55B15 (an example of the type a-TM1xTM2y Bz ;\nTM - transition metal) was investigated. Hydrogen concentration c H was measured by Sieverts method in\ntemperature interval from T = 150 °C to T = 350 °C under hydrogen pressure p up to 6 MPa. It was found that\nc H was an increasing function of p and its maximum value was typically 0.5 wt.% H2 at 350 °C and 6 MPa.\nHowever, when the alloy was preliminary hydrogen charged (PHC), the pressure dependence of total c Htot in\nthe first absorption cycle(s) is non-monotonous in dependence on PHC conditions. For the sake of comparison,\nthe same absorption characteristics were measured also in Mg2Ni intermetallic that is a common constituent\nin Mg-based HSMs. Comparing Co30Fe55B15 and Mg2Ni, it was concluded that Co30Fe55B15 shows lower\nhydrogen solubility, but much better absorption kinetics.
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Fluidized-bed Combustion of Dry Stabilized Sewage Sludge in Oxy-fuel Mode
Moško, Jaroslav ; Pohořelý, Michael ; Durda, Tomáš ; Zach, Boleslav ; Šyc, Michal ; Svoboda, Karel
Sewage sludge is an inevitable waste stream from wastewater treatment and its disposal is complicated due to specific characteristics of its composition. Sewage sludge combustion/incineration is the only suitable way for disposal of large volumes of sewage sludge containing POPs and some heavy metals. Sewage sludge combustion reduces the volume of such waste and causes destruction of organic contaminants. Oxy-fuel combustion is a technology for simplifying CO2 sequestration from the flue gas by using nearly pure oxygen instead of air for the combustion. In order to control the combustion temperature, part of the flue gas is recycled to combustion chamber. The paper brings information about investigation of the effects of combustion temperature and concentration of oxygen in inlet combustion media on the emissions of NOx, N2O and SO2 in FB combustion of sewage sludge. The experimental results have shown that an increase in combustion temperature leads to an increase in NOx and to a decrease in N2O emissions in the temperature range 750–930 °C. The lowest SO2 emissions were measured in the temperature interval from 800 to 820 °C in all experiments with different oxygen concentration in inlet gas. It was found that when the oxygen concentration in inlet gas increased, the emissions of SO2 increased while emissions of NOx slightly decreased.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_C22016112214480 - PDF
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NUMERICAL ESTIMATION OF MICRO-CRACK PATHS IN POLYMER PARTICULATE COMPOSITE
Majer, Z. ; Náhlík, Luboš
Determination of composite mechanical behavior is one of important part during the composite tailoring. The aim of the present work was to estimate a micro-crack behavior in a polymer particulate composite. The composite was investigated by means of the finite element method -using ANSYS software. A two-dimensional three-phase finite element model was developed to analyze the crack growth behavior. The assumptions of the linear elastic fracture mechanics were considered and the Maximum Tangential Stress (MTS) criterion was used to predict the direction of the crack propagation. The effect of the elastic modulus of the interphase on the micro-crack propagation was investigated. The properties of matrix and particles were taken from experiment. It was shown that the interphase properties influence the stress intensity factor KI as well as the micro-crack paths. The results of this paper can contribute to a better understanding of the micro-crack propagation in particulate composites with respect to the interphase.
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Age management
Jirásková, Barbora ; Dvořák, Marek (advisor)
This thesis processes the concept of the human resources management with respect to the age structure of employees in the intentions of the Czech Republic. In the theoretical part, there is outlined the history of the application of the principle of age management, focusing on current demographic trends, forecasts of population age structure, the aging workforce and the need to preserve the ability to work into old age. Points to the need to adapt corporate strategy, personnel management trend of an aging population. It presents ideas for the main underlying programs and legislative measures which form the basis of the latest concepts and strategic plans in the Czech Republic and Europe. The empirical part deals with a specific level of allowance organization established by TSU and two sub investigation. The first is a case study of age management staff. The resulting data are confronted with the results of a survey among employees of the organization. The second investigation are interviews with members of management and employees of the HR department on the issue of the introduction of age management, their awareness of the concept of age management, the question of age discrimination, the issue of maintaining the continuity of knowledge in the organization and their views on these topics. The outcome of this survey indicates a willingness and readiness of the organization has already received age management principles adhere to and develop further.
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