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Analysis history communication xChat
RYNEŠ, Pavel
Based on the needs of forensic examination of communication history of various communication programs for the police authorities and forensic experts is needed documentation chat history of "xChat". This is especially necessary to analyze the data files containing records of contacts and communication history at a local PC. Based on the contact information to analyze the possibility of obtaining identification information from the portal xChat
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název v anglickém jazyce není uveden
Ulmanová, Olga ; Růžička, Evžen (advisor) ; Bojar, Martin (referee) ; Stančák, Andrej (referee)
Essential tremor (ET) is probably the most common movement disorder with prevalence quoted as ranging in various populations from 0,41 to 3,92% (Louis et al 1998). Despite often given the prefix "benign", many patients are seriously physically, socially or psychologically handicapped. There are sporadic and familial forms with autosomal dominant inheritance with high penetrance. The percentage of patients with a positive family history ranges from 17 to 100%. Etiology and pathogenesis of ET are unknown. Electrophysiological studies have shown the central source of tremorogenic oscillation, the cerebellum and inferior olive are implicated by positron emission tomography studies. Autopsy studies reveal no gross or microscopic abnormalities (Deuschl and Elble 2000). Essential tremor had been for a long time one of the diseases that were not at a primary focus of neurological research (Deuschl and Koller 2000). Now, with the rapid progress of genetic research, the first genes related to ET have been identified (Gulcher et al 1997, Higgins et al 1997). ET is probably not a single disease; several genetic abnormalities appear to exist (Illarioshkin et al 2000, Abbruzzese et al 2001, Kovach et al 2001). High-quality epidemiological studies are needed for the search for genetic abnormalities (Elble 2000). The...
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Legal policy of genetically modified organisms and Consumer Protection
Skálová, Petra ; Neděla, Radek (advisor) ; Bačina, Jan (referee)
This Bachelor thesis is focused on genetic engineering and its results. The aim is to familiarize the reader with the issue of biotechnology and its products, which are genetically modified organisms. The introduction of the work explains the basic concepts and describes the history of the biotechnology and genetic modifications in general. In this part are also listed examples of genetic modifications including information about testing and practical application in the Czech Republic. This scientific branch is in the very beginning and there are still ongoing series of programs and research in order to create new plants and animals. The second chapter briefly summarizes the positive and negative aspects of the use of genetic modification. The third chapter is focused on the legal regulation of this issue. The first subsection summarizes the Czech legislation, the second one summarizes European legislative shared by member states of the EU. Fundamental international organisations which are dealing with genetic engineering are not omitted in following subsection. Some international agreements are also mentioned. The last, it means fourth, chapter focuses on the consumers, not only as an object of the protection by government and international organisations, but also in terms of their attitude to genetically modified organisms used in the production process of food intended for the market. Subsections describe the issues of labelling GM-products and lack of awareness of people, they mention movements and non-profit organisations fighting against using GMOs in the products intended for human consumption and their use in general. This issue is connected with many risks including possible side-effects on human health. This section also describes general attitude of the consumers themselves to GM-products.
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Phylogeography and conservation genetics of endangered saproxylic beetles in Europe
DRAG, Lukáš
This thesis introduces the use of molecular methods for the conservation of several species of endangered saproxylic beetles in Europe. It focuses on the questions related to the DNA preservation and microsatellites development, as well as the evolutionary history and conservation of threatened species. Using the combination of mitochondrial and nuclear markers, the genetic diversity and reintroduction history of Cerambyx cerdo was assessed and the phylogeography of Rosalia alpina from the whole range of its distribution was studied. This information is valuable for designing more efficient conservation strategies.
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Molecular detection RYR1 gene mutation causing malignant hyperthermia in dogs
Krausová, Klára ; Vejl, Pavel (advisor) ; Melounová, Martina (referee)
The theme of this work are methods for molecular detection of mutations in the dog genome causing malignant hyperthermia.
The work is divided into two main parts. The first part is a literary research summarizing knowledge about malignant hyperthermia from the human and veterinary anesthesiology molecular biology and genetics. The second part of the work describes the methodology and conclusions drawn from the molecular detection of above mentioned mutation which was conducted in the laboratory of the Department of genetics.
The literary research deal with malignant hyperthermia syndrome. The aim is to familiarize with symptomatic manifestations triggers the mechanisms the development of acute malignant hyperthermia and its clinical diagnosis. The first cases of observation of malignant hyperthermia syndrome are also described as well as the history of the research which led to a detailed description of this disease in humans dogs and other species bearing malignant hyperthermia causing mutation.
The next part of literary research is a summary of knowledge about the mechanism of mutation the detection of sequences containing this mutation and influence on the arrangement of proteins in the ryanodine receptors.
For the last part of literary research commonly used laboratory techniques of non invasive mutation detection were chosen. These techniques are mostly used by accredit genetics laboratories. Listing of Czech laboratories performing diagnostic of gene RYR1 mutation is also contained.
Experimental part of barchelors thesis is focussed on molecular marker optimalization for casual mutation in RYR1 gene detection by technique of restriction fragment length polymorphism (PCR RFLP). This technique was apply to 338 deputies of different dog breeds. Accomplished analysis did not confirm presence of the mutated allele in assessed breeds population. Result of PCR RFLP technique was confirmed by sequence analysis.
Results were discussed with literary sources which did not judge real breeds but are developing a model special bred line with presumed mutated allele segregation. The results indicated that the mutation of gene RYR1 do not represent an influential disorder in breeds harbored in Czech Republic. Sporadic presence of this mutation was consulted with commercial genetic laboratories.
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Risk factors for malignant neoplasms of skin and prevention options
Rosina, Jozef ; Čelko, Alexander (advisor) ; Kolářová, Hana (referee) ; Dohnal, Karel (referee) ; Navrátil, Leoš (referee)
Skin tumors are the most common types of tumors in humans. Their incidence is steadily increasing. Increases and mortality, especially in malignant melanoma. Risk factors playing an important role in the development of skin cancer, solar radiation belong. UV spectrum of sunlight is the most important cause of skin cancer. Therefore, the degradation of the ozone layer and the associated increased levels of UV radiation reaching the world so much studied. Among other known risk factors belong phenotype of human acute intermittent sunburn, especially in children and adolescents, increased tendency to form freckles skin after exposure to sunlight and the presence of pigmented nevi. Some people may find a genetic basis. The risk of malignant melanoma is also rising in people who have a history had already been diagnosed with this disease. Significant for the possibility of skin malignancy is immunosuppression. The work is focused on statistical analysis of most of the above mentioned risk factors.
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Postglacial colonization of black alder (Alnus glutinosa) and grey alder (Alnus incana) in Europe
Havrdová, Alena ; Mandák, Bohumil (advisor) ; Helena, Helena (referee)
Current species distribution in Europe was mainly influenced by massive climatic and environmental changes during the Quaternary period. Different theories concerning survival of tree species during the last ice age in Europe were proposed and up to date the position of glacial refugia and directions of migration routes are under active debate. My dissertation thesis aimed to combine information from fossil records and genetic analysis to improve knowledge on postglacial history of Alnus glutinosa and Alnus incana. The synthesis of palaeoecological data supports the idea that not only southern but also northern populations were important sources of postglacial Alnus expansion. The delayed Alnus expansion apparent in some regions was likely a result of environmental limitations. Our findings from molecular study showed differences in postglacial histories between temperate A. glutinosa and boreal A. incana. In the case of A. incana, we found an effective refugium in Central Europe located outside classical southern refugia confirming the existence of northern refugia for boreal trees in Europe. Fennoscandian populations are derived from Central-European ones that originated from populations in the Alps. For A. glutinosa, multiple southern refugia were revealed and three main directions of postglacial expansion were proposed: 1) from the northern part of the Iberian Peninsula to Western and Central Europe and subsequently to the British Isles, 2) from the Apennine Peninsula to the Alps, and 3) from the eastern part of the Balkan Peninsula to the Carpathians followed by expansion towards the Northern European plains. It has been shown that colonizing lineages have met several times and formed secondary contact zones with unexpectedly high population genetic diversity in Central Europe and Scandinavia. For the first time, we discovered tetraploid populations of A. glutinosa situated in the putative main glacial refugia on the Iberian and Balkan Peninsulas. Neither of them was probably involved in the colonization of Central and Northern Europe after glacial withdrawal. In conclusion, thanks to extensive population sampling, testing of hypothesis postulated based on fossil data by molecular data and using two molecular markers, i.e. chloroplast DNA and microsatellites, with different mode of inheritance and polymorphisms, this project revealed not only the position of glacial refugia of European tree species and discriminate between effective and non-effective ones, but also help infer the main migration routes. This approach enabled us to change some long-lasting paradigms and brought new pieces of knowledge about postglacial colonization of European tree species.
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Complex Treatment of Patients with Bechterew´s Syndrome
ARCIMOVIČOVÁ, Petra
The bachelor thesis titled "Complex Treatment of Patients with Bechterew´s Syndrome" suggests therapeutic possibilities of the disease. Bechterew´s syndrome is a frequent topic of scientific papers but the complexity of treatment is not always considered. A major part of the theoretical section presents the disease with its signs and symptoms, and also discusses the much debated causes, the progression and phases of the disease. The thesis focuses on the psychosomatic approach; it discusses potential influences and other factors, specifically genetic and antigen factors, a potential connection with bacterial diseases, the function of intestinal microflora and its relation to dietary habits. Another important topic is influence of specific personality traits, as well as the implication of the patient´s social interactions in the early childhood on the behaviour in an adult age. The research results in five examinations of patients with Bechterew´s syndrome including personal history, kinesiology examination and a two-hour interview with each patient. The data are qualitatively analyzed. There are two research questions: the first explores which physical, psychological, social and spiritual factors may improve the disease; the second looks into factors which may aggravate it. On the one hand, in the majority of cases the most important factors seem to be the diet, physical activity, specific personality traits and faith in God. On the other hand, the diversity of patients - interviews with patients taking place in a spa, a rehabilitation centre, at home - leads to very different personal opinions and feelings. The last interview, number 6, offers a homeopathic point of view. In spite of the fact that the research sample is relatively small, the research data are very useful, even though no definite conclusions can be made. The thesis may contribute to the knowledge of health workers specializing in the treatment of Bechterew´s syndrome and to the training of physical therapists.
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CEA in breeds used for dog-assisted activities and therapies
Tipková, Zuzana ; Sedláková, Vladimíra (advisor) ; Fiala Šebková, Naděžda (referee)
Collie Eye Anomaly (CEA) is an inherited disease that primarily affects herding dog breeds such as Smooth and Rough Collie, Border Collie, Shetland Sheepdog and Australian Shepherd, as well as Nova Scotia Duck Tolling Retriever. All these breeds can be used for dog-assisted activities, some individuals are also suitable for dog-assisted therapies. In the literature I found general information about the history, symptoms, diagnosis, mode of inheritance, genetic testing of the predisposed breeds.
CEA was first described in the 50s of the 20th century. Manifestations of CEA vary widely and therefore it has not been known that a variety of symptoms is caused by one disease for a long time.
Choroidal hypoplasia is the characteristic sign, furthermore may be present: coloboma, tortuosity of the retinal blood vessels, intraocular hemorrhage and retinal detachment leading to blindness. CEA cannot be cured. It is recommended to perform an early diagnosis, which is possible from 6 to 8 weeks of age, because the defect can be covered by later pigmentation in older pups and ocular fundus of the dog then appears normal. At first dog breeders did not pay much attention to this anomaly, because it is a small percentage of individuals who become completely blind as a consequence of that disease. Carriers of CEA have no symptoms, but their offspring may inherit a severe form of CEA. We cannot exclude affected individuals from breeding program in the breeds with high prevalence of CEA since it would reduce genetic diversity.
It is an autosomal recessive disease caused by mutation in the NHEJ1 gene. After the causative mutation was found, a two-step PCR assay, which uses two primer pairs to quickly detect the mutation in chromosomes, was developed. The latest molecular methods for the diagnosis of CEA include simplified analysis of the deletion without DNA isolation and rapid genotyping technique based on SYBR Green real-time PCR. Genetic tests are available in many countries now and so breeders can control the health of their dogs.
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Family Muscidae on large cadavers with the beginning of decomposition in the winter and its use in forensic practice
Dočekalová, Tereza ; Barták, Miroslav (advisor) ; Klimešová, Vanda (referee)
Forensic entomology is a one of the special fields of criminology. That uses knowledge of biology, ecology and especially insects and other invertebrates, which occur on cadavers or in its vicinity. These and other arthropods are used as circumstantial evidences during the investigation and also verify other evidence in civil and criminal law.
This bachelor thesis deals with the use of Diptera of forensic practice with focusing
on family Muscidae by form of a scientific work. The first part of the thesis is focuses
on history and current use of forensic entomology, advances in characterization of thematic cases in which the family Muscidae was used as well as describes main species of the family. The second part describes the field experiment in which the presence of the family Muscidae on model organism (pig carrion) was studied and its use in forensic practice.
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