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Canistherapy and Hypotherapy As the Treatment Method on the Handicapped People
Pittnerová, Monika ; Ortová, Marie (advisor) ; Drahotová, Jana (referee)
The work describes two zootherapy alternatives - canistherapy and hypotherapy. At first; on the theoretical level intents on course of the therapy, on the selection of the suitable animals and on the choice of the experienced therapeuts. Canistherapy and hypotherapy take advantage of the children, of the handicapped people and the seniors. On basis of practical experience is described the course and the advantage of this therapy with children who suffer from Cerebral palsy. In the conclusion of this work both these animotherapies are compared. Powered by TCPDF (www.tcpdf.org)
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Photodynamic therapy. Instrumental optics
Votruba, J. ; Stádník, Bohumil ; Javorský, Stanislav
Photodynamic therapy of cancerous tumorous of various human organs uses both coherent and noncoherent light sources for the treatment. However, noncoherent ones are prefered due to higher treatment efficiency, simpler and reliable operation and lower financial costs. Main purpose of this report is to give overview of basic optical methods and instruments used in the photodynamic therapy.
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The possibilities of use of the robotic system da Vinci for treatment of localized prostate tumor
Podzimek, Michal ; Kočárek, Jiří (advisor) ; Kolombo, Ivan (referee)
Cancer of prostate is the most frequent male malignity and also it is the second most common cause of death in Europe and in the USA. There are 2 228 new cases reported in the Czech republic per year. The probability of occurrence of this disease is one to six during whole life. There is a good success in detection of his disease in the early stage due to development of screening methods. In such case the radical prostatectomy is very successful method of treatment. Besides of operation methods like open radical prostatectomy and laparoscopic prostatectomy there is a new operation method using robotic system da Vinci. The aim of this machine is to bring the advantages of the miniinvasive surgery together with keeping intuitive handling like in open surgery. First da Vinci system was bought and installed in the Central military hospital in Prague, Czech republic in 2005. The common knowledge about using this method is very low in both general public and specialists. Nor patients, neither their doctors often know about possibility of surgery using da Vinci system. The main objective of this thesis is to evaluate almost three years of experience of treatment localized prostate tumor using da Vinci system, and to describe its possibilities, benefits and disadvantages. Collection of 255 patients files aged...
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T cells labelling by bimodal contrast agent for in vivo studies of stroke
Krijt, Matyáš ; Poljaková, Jitka (advisor) ; Kříž, Jan (referee)
Stroke is a serious brain injury, which causes sudden death or terminates in permanent neurological disability. Nowadays, tissue plasminogen activator (tPA) is used as the only effective treatment of stroke. One of the potential targets for novel therapy are T cells. Even though the explicit role of T cells in the pathogenesis of brain injury, amounts and timing of all T cell subtypes infiltrating into brain during the stroke still needs further investigation. The research in this field is complicated by the lack of efficient methods for in vivo cell tracking. Therefore the aim of this thesis was to develop a method of T cells labelling by MRI contrast agent in order to investigate T cells distribution in ischemic mice model using in vivo MR imaging. T cells were isolated from C57/BL6 mice in two step isolation protocol using gradient centrifugation and magnetic separation with the efficiency of 97 %. The isolated cells were labelled with 100 μg Fe/mL of Molday ION Rhodamine B contrast agent. The labelling efficiency after 17 hours of cells incubation was higher than 99 %. The labelled cells were cultured with CD3 and CD28 antibodies resulting into the 74 % viability of labelled T cells compared to 83 % viability of non labelled T cells. The labelled T cells were visualized by fluorescent...
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Genetic Aspects of Sporadic Ovarian Cancer
Jančárková, Natalia ; Freitag, Pavel (advisor) ; Rokyta, Richard (referee) ; Kohoutová, Milada (referee)
58 9. SUMMARY Molecular biological parameters, including genetic alterations, present new and perspective direction in diagnostics, prediction of prognosis, monitoring and possible therapeutic approaches in oncological disease. The results presented have to do with the project, which is concentrated upon chromosomal rearrangements in ovarian and cervical cancer and their correlations with available parameters of both molecular biological and clinical characteristics. Sixty patients with ovarian cancer and twenty patients manifesting cervical cancer were included into the study. The histological type and grade, MIB-1 and p53 (using immunohistochemical method) were estimated by histopathologist. Both conventional karyotyping and molecular-cytogenetic methods (fluorescent in situ hybridization and comparative genomic hybridization) were applied to reveal chromosomal aberrations. The results were subjected to statistical evaluation, using analysis of variances and χ2 test. There were correlated parameters of quantitative and qualitative character - age, stage, histological type, grade, CA 125 before and after treatment, MIB-1, p53, surgical residuum, lymphadenectomy, response rate, chromosomal rearrangements. Analyses accomplished in ovarian cancer group revealed typical amplifications on chromosomes 1q, 3q and...
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The process of addiction treatment in the fellowship of Alcoholics Anonymous
Němcová, Lucie ; Grygar, Jakub (advisor) ; Hájek, Martin (referee)
This thesis concerns an alternative approach to the alcohol-addiction treatment within the fellowship of Alcoholics Anonymous and highlights the importance of self-help groups in the contemporary society. This worldwide biggest self-help group uses sociologically important processes to achieve a new way of life connected with sobriety. I have studied these processes through the participant observation. Alcoholics Anonymous influences its members through its specific culture which reflects in group rituals, high level of group affiliation, spirituality and co-constructed community story, which bears the member's knowledge. This thesis looks on the transition to identity of the sober alcoholic and the new way of living according to Twelve-Step program of Alcoholics Anonymous through the perspective of drift model of conversion, transition rituals or principles used in the narrative therapy. Within these processes there occurs radical reconstruction of perceptual, cognitive and behavioral schemes which influence the meanings in the interpretation of everyday life and consecutively change the identity of the individual and his social action. These changes of the individual are strengthened by the group members and then gradually spread out of the group boundaries too.
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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Biologically Active Metabolites of Plants IV. Alkaloids from Chelidonium Majus L. and Their Neurotropic and Antioxidant Activity
Novotná, Magdalena ; Cahlíková, Lucie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Novotná Magdalena, Mgr. Consultant: Cahlíková Lucie, Ing., Ph.D. Thesis title: Biologically Active Metabolites of Plants. IV. Alkaloids from Chelidonium majus L. and Their Neurotropic and Antioxidant Activity The aim of this thesis, which follows the diploma thesis, was to process the second half pool of alkaloids of Chelidonium majus, chlorides insoluble in chloroform, to isolate the remaining alkaloids in a pure form, to test their biological activity against two human cholinesterases (HuAChE, HuBuChE) and to determine their antioxidant activity. After division by column chromatography, the two alkaloids were purified using preparative chromatography. Based on structural analysis (NMR, MS) and comparison of data from the literature, the substances have been identified being chelidonine and homochelidonine. The activity of both alkaloids against two human cholinesterase (HuAChE, HuBuChE) was determined. Experimentally obtained IC50 values were compared with the standard drugs, that are used in the treatment of the Alzheimer's disease (galantamine and huperzin A). Chelidonine exhibited promising IC50 values towards HuAChE and HuBuChE (IC50 26.8±1.2 µM HuAChE and 31.9±1.4...
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