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Splicing of atypical introns in S. cerevisiae
Cit, Zdeněk ; Půta, František (advisor) ; Pichová, Alena (referee)
Pre-mRNA splicing is a vital process of gene expression important for all eukaryotic organisms. For the proper function of this very complex and dynamic event the presence of few specialized RNA and many proteins that hold a variety of tasks is necessary, not only inside the splicing complex itself, but also beyond this complex. The Prp45 is one of the proteins involved in pre-mRNA splicing in yeast Saccharomyces cerevisiae. Its human homologue, SNW1/SKIP, is involved in splicing but also in other crucial cell processes. The Prp45 protein was reliably reported only to participate in the second transesterification reaction of splicing. But there are also data suggesting its possible involvement in the first transesterification reaction. This work provides further evidences linking protein Prp45 with the first splicing reaction, obtained by the research of cells carrying the mutant allele prp45(1-169). Cells carrying this allele show dropped splicing and accumulation of pre-mRNAs. This thesis therefore also investigated the possible influence of Prp45 protein on the RNA export from the nucleus to the cytoplasm. But no connection between this protein and RNA transport was discovered. Keywords pre-mRNA splicing; Saccharomyces cerevisiae; Prp45; Mer1; Mud2; Prp22; Rrp6; AMA1; SNW1/SKIP
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Multicriteria games
Tichá, Michaela ; Dlouhý, Martin (advisor) ; Lachout, Petr (referee) ; Čičková, Zuzana (referee)
Theory of multicriteria games is a special field of game theory, when one or more players have at least two payoff functions and want to maximize simultaneously. The work introduces a number of new findings. It examined the concept of finding equilibria in pure strategies in noncooperative multicriteria game. It is possible to find all the equilibria in pure strategies by full search and solving two linear programs for each point. Furthermore, two linear programs are formulated for verifying that a selected point is the equilibrium of the game or not. In the noncooperative games is also introduced the concept that with knowledge of the equilibrium of bimatrix game determines preferences of the players. Although finding the equilibrium point of the bimatrix game is nonlinear problem, finding the preferences is linear problem. The latest findings in the noncooperative games is a generalization of the concept that solves multicriteria game by assigning weights to each criterion of each player. The work demonstrates that it may not be necessarily linear weights, but it can be more general function that describes the player's preference. The remaining part is devoted to knowledge in cooperative games. There is considered that the players know their preferences and are able to express them by weights. The game with known preferences is defined and solved with the use of bargaining theory. Then it is generalized to a case where players have more payoff functions, from which they can choose. Finally, the multicriteria case of voting game is defined. It is designed completely new concept, which selects the winning coalition in the voting game. This concept is then applied to the real situation after the elections to the Chamber of Deputies in 2013.
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Business plan for setting-up of language school
Kahánková, Andrea ; Chylíková, Hana (advisor) ; Matysová, Kamila (referee)
The aim of this bachelor thesis is to develop a business plan for a set-up of a company specializing in English language tutoring. The theoretical part defines basic rules of doing business in the Czech Republic. Furthermore, it explains the recommended structure of a business plan and provides an alternative model for its development called Lean Canvas. Theoretical part of the business plan is subsequently applied in the practical part for elaboration of a business plan for the chosen field of activity. The financial plan is provided in three versions (realistic, optimistic and pessimistic), which have all been subsequently evaluated. Lean Canvas for the planned business in language tutoring has been developed at the end of the practical part. The conclusion provides summary of the findings of all business options and provides recommendations for the prospective project implementation.
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Evaluation of the educational systems in the companies in the Czech republic
Daňhelková, Barbora ; Křečková Kroupová, Zuzana (advisor) ; Cetkovský, Pavel (referee)
Employee education alongside with skilled employees motivated to further development is currently becoming both an important part in the development of companies and a significant competition advantage in all markets worldwide. The aim of this thesis is to analyze educational systems in Czech companies and to evaluate the educational process of employees. The thesis is divided into two parts, theoretical one and practical one. The first part deals with defining theoretical concepts related to education, the theory of corporate education and trends in corporate education. The second part is focused on the research of corporate education in Czech companies. The research is based on the internal documentation and interviews with the HR representatives in the company HOCHTIEF CZ, and interviews with experts in the field of corporate education from the company DMC management consulting.
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Optimal production strategy of the chosen company in the area of mechanical engineering
Brachtlová, Veronika ; Průša, Přemysl (advisor) ; Všetečková, Dana (referee)
The topic of this diploma thesis is "Optimal production strategy of the chosen company in the area of mechanical engineering" - namely the EUTECH join stock company, or more precisely its toolshop division, which deals with the manufacture of injection moulds, parts and production of special devices for aircraft industry. The aim of the paper is to evaluate, according to the defined factors, what is for the EUTECH join stock company more favourable - invest in purchase of the 5-Axis CNC machining center technology and deal with this process inside of the company or ensure this technology in cooperation with another companies. The thesis is divided into two main parts - theoretical and practical. In the theoretical part, the subjects of operation management theory and production are dealt with. The practical part deals with the production strategy of the EUTECH join stock company, or more precisely of its toolshop division in detail. The company is briefly introduced, the situational analysis of division toolshop is conducted and the production process of the company is examined. The final chapter deals with the use of cooperation in the toolshop division. The main and other factors affecting the choice of "product or sell" strategy are evaluated and consequently is decided which strategy is in case of the 5-Axis CNC machining center technology more favourable.
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Dance education of preschool hyperactive children
Lacková, Ivana ; LÖSSL, Jiří (advisor) ; FRIČOVÁ, Marie (referee)
The topic of this diploma thesis is Dance education of preschool hyperactive children. Goal of research is to describe teaching of hyperactive children especially dance lessons.
There is described Attention Deficit Hyperactivity Disorder in the first part of thesis. There is written about special needs of children with ADHD focused on education.
Second part is focused on practical dance lesson of ADHD children. There is described example dance lesson based on theory and practical experience of experts.
The result of research is that supply of special lessons is very limited and it is big pity. In the diploma thesis was recognized that the education with special program should help to children with ADHD and it can be good supplement to standard medical therapy.
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FAUST AND JOB´S
Slezáková, Kateřina ; ULLRICHOVÁ, Daria (advisor) ; SÍLOVÁ, Zuzana (referee)
This thesis examins the nature of Faust’s inner ambivalence in the context of modern times. It tries to identify the basic traits leading to the feeling of emptiness. The authoress uses comparing the dramatic character of Faust with his biblical prototype of Job and one of the most successful entrepreneurs today Steve Jobs. Based on the comparison she contemplates the validity or mutation of the drawing theses nowadays. In the second part the authoress reflects the production of the project of Faust. Due to her specialization in the field of dramaturgy she pursues especially the process of the selection of particular dramatic situations, adjustments of dramatic text and the choice of suitable staging means.
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Documentation of the nature reserve Miletinska bazantnice (Czech Republic)
Stejskalová, Klára ; Skalický, Milan (advisor) ; Martinková, Jaroslava (referee)
The aim of this study is to document the floristic species diversity through research in the natural reserve Miletinska bazantnice. Documentation was carried out on the territory concerned during the growing season of 2015. There was a determination of vascular species. The research was mainly focused on endangered and protected species.
The whole area is part of sites of European importance under the Habitats Directive Natura 2000 Miletinska bazantnice was declared a nature reserve in 1954 with an area of 75, 43 hectares and is part of a small-scale specially protected area. Typical features of the area are remnants of natural Podzvicinsko geobiocenosis and therefore the reason for its protection.
Further it describes the history, characteristics of the territory and the natural conditions that are typical for the locality. These include soil science, climate, hydrological, geomorphological, geological and phytogeographical. On the territory of the nature reserve are recorded two memorial trees. It´s oaks (Quercus robur) called Zizkovy duby.
At present territories were found in protected and endangered species and classified under the Red and black list of vascular plants of the Czech Republic (Grulich, 2012) and Decree of the Ministry of Environment of the Czech Republic no. 395/1992 Coll. Into the category of rare taxa that require additional attention include species such as Carex otrubae, Cerastium lucorum, Daphne mezereum, Galium boreale, Listera ovata and Primula veris. The only species Platanthera bifolia is ranked in the category of endangered under both the Red and black list of vascular plants in the Czech Republic and under Decree.
In the field survey, attention was also paid to invasive species, sorted by Catalogue of alien plants of the Czech Republic (Pyšek et al., 2012) and categorized archeophytes (Arctium tomentosum, Cirsium arvense, Convolvulus arvensis, Chelidonium majus)
and neophytes (Arrhenatherum elatius, Impatiens parviflora) and described their risks (Mlíkovský and Stýblo, 2006)
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Individual human odor as a forensic trail in criminal proceeding made by contact or contactless transmission and its resistance to physical agents
Santariová, Milena ; Bartoš, Luděk (advisor) ; Chmelíková, Eva (referee)
The dissertation thesis consists of four papers that have been published in scientific journals.
Study n. 1
The need to recover evidence from water is quite common in criminal investigation. The article deals with the possibility to collect human scent from such objects and with the ability of specially trained dogs to match such scent samples with scent samples collected from detained suspects. During an experiment, designed as a blind one, it has been proved, that human scent can survive on submerged objects and can be later used for scent identification.
Study n. 2
To collect odors the Czech Republic Police use special fabric sorbents manufactured under the registered mark ARATEX. Before use the fabric sorbent is treated by water vapor sterilization. After the scent identification the sorbent is destroyed. The goal of the study was to verify if the vapor sterilization is effective enough to remove human scent that has already penetrated into the sorbent structure or in other words if the sorbent can be exposed to vapor sterilization and then used again. Specially trained dogs were used to match starting odors with target odors in the line-ups. The results showed that dogs are able to correctly match human odors even after they have being exposed to vapor sterilization.
Study n. 3
The purpose of the study was to determine the temperature at which the human scent is degraded so that a dog would not be able to identify it. In contrast to expectations, eight dogs used in the experiment almost flawlessly identified human scents from five scent donors exposed to temperatures of 100°C, 200°C, 300°C, 400°C, 500°C, 600°C, 700°C, and 800°C. Only two of the dogs were able to identify 5 of 15 scent samples exposed to 900°C. No dog identified a scent exposed to 1000°C. Our study verified heat survivability of human scent far beyond existing expectations.
Study n. 4
We tested the hypothesis that if odor fallout (the release of a human odor onto an untouched object) in human subjects exists, then holding a hand above an absorbent will produce a detectable scent which will be subsequently matched in a detection test by trained dogs. Scents were collected from seven males to sterile cotton absorbent squares. The left hand was used to get the control scent and the right hand served as the target scent. Each experimental subject was sitting and his left hand was laid down on a cotton square for 3 min. The right hand was held 5 cm above another cotton square for 3 min. The scent identification was done by two specially trained police German shepherds. Both dogs performed 14 line-ups and correctly matched the collected scents of all test subjects. The results suggest the existence of human odor fallout, whereby a human scent trace is left by humans even if they do not touch an object.
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