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Analysis of the decision-making factors in freight forwarder selection process
Šrámková, Eva ; Novák, Radek (advisor) ; Cempírek, Václav (referee) ; Průša, Bohumil (referee)
The thesis analyses the factors of customer decision-making process by choosing a freight forwarder in the Czech Republic market. While applying factors by other relevant research studies dealing with the decision-making process of customers in non-European countries, the research examines factors characteristic for the Czech customers. Questionnaires and in-depth interviews as research methods are applied. Within the research, a new service quality instrument TARRQUAL was developed. The TARRQUAL was proved on a survey including 250 customers of freight forwarder Kühne+Nagel Czech Republic. As service quality measurement, TARRQUAL is supposed to be applied particularly in logistics business field. The project aims at providing input for empirical research in the field of service quality in container shipping, which is lacking in the Central and East European countries.
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Synura echinulata (Synurales) - silica scale plasticity in natural populations influenced by environmental factors; accompanying scaled chrysophytes
Kreidlová, Jana ; Němcová, Yvonne (advisor) ; Hodač, Ladislav (referee)
The silica scale shape variation in Synura echinulata has been investigated only in laboratory conditions, so far. The aim of this thesis was to study scale shape variation in natural populations and to determine which environmental parameters influence the scale shape. The scale shape variation was investigated using landmark-based methods of geometric morphometrics. Scale shape changes related to environmental factors were analyzed using the two-block method PLS (Partial Least-Squares analysis) and adonis (R). The scale shape was mostly influenced by locality, pH and altitude. Shape distinguished populations of S. echinulata probably exist in different localities. The more distant localities are, the less similar are scales of S. echinulata in their shape. This scale shape variation is probably genetically determined, even thought all so far sequenced populations belong to the same species. High morphological disparity which was probably caused by anthropogenic pollution, was recorded in the locality Brdy. During sampling of the natural populations of Synura echinulata in the Czech Republic, several localities representing a significant reservoirs of species diversity, were found. Therefore, the thesis was extended and biodiversity research in the Czech Republic was added. A few samples were...
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T cells labelling by bimodal contrast agent for in vivo studies of stroke
Krijt, Matyáš ; Poljaková, Jitka (advisor) ; Kříž, Jan (referee)
Stroke is a serious brain injury, which causes sudden death or terminates in permanent neurological disability. Nowadays, tissue plasminogen activator (tPA) is used as the only effective treatment of stroke. One of the potential targets for novel therapy are T cells. Even though the explicit role of T cells in the pathogenesis of brain injury, amounts and timing of all T cell subtypes infiltrating into brain during the stroke still needs further investigation. The research in this field is complicated by the lack of efficient methods for in vivo cell tracking. Therefore the aim of this thesis was to develop a method of T cells labelling by MRI contrast agent in order to investigate T cells distribution in ischemic mice model using in vivo MR imaging. T cells were isolated from C57/BL6 mice in two step isolation protocol using gradient centrifugation and magnetic separation with the efficiency of 97 %. The isolated cells were labelled with 100 μg Fe/mL of Molday ION Rhodamine B contrast agent. The labelling efficiency after 17 hours of cells incubation was higher than 99 %. The labelled cells were cultured with CD3 and CD28 antibodies resulting into the 74 % viability of labelled T cells compared to 83 % viability of non labelled T cells. The labelled T cells were visualized by fluorescent...
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Dietary supplements and iodine supplementation for children 11-12 years in the Czech Republic
HALÁSZOVÁ, Monika
Bachelor Thesis called "Dietary supplements and iodine supplementation for children 11-12 years in the Czech Republic" deal with the intake of dietary supplements containing iodine and intake of other significant dietary sources of iodine in relationship to saturation with iodine in the monitored group of 300 children aged 11-12 years . The thesis is divided into theoretical and practical part. Theoretical part deal with established issues in light of current situation and uses the knowledge of specialized literature in these areas: iodine and his dietary sources, influence of deficient or excessive intake of iodine on thyroid gland and its function, and also the solution to iodine deficit in Czech Republic. The thesis also deals with dietary supplements and nutrition of school-aged children. Practical part is focused on quantitative research. This research was done on selected group of children who had low level of ioduria (iodine in urine) according to State health institution. Statistical file included 300 children of both gender in age from 11 to 12 who live in Czech Republic. The reason why children of this age were chosen is because of importance of optimal intake of iodine in period of physical and sexual evolution. The children were divided into two groups. One group had ioduria up to 300ug/l and the second group had ioduria over 300u/l. Data were collected through questionnaire. Because of age of respondents, the questionnaire was filled in presence of at least parent. The questionnaire should have also served to parents as information about iodine importance for their children. The individual evaluation of ioduria and questionnaire was given to parents upon their request. Collected data were evaluated in Microsoft Excel. In practical part, two main goals and two research questions are set. Their task is to determine the rate of usage of dietary supplements with iodine and to find out what significant sources of iodine the children with ioduria over 300u/l take in. The milk and milk products, salt with iodine, salted delicacies, sea products and dietary supplements with iodine were observed as probable main sources of iodine intake. The first goal was to find out the amount of iodine intake from dietary supplements for previously mentioned group of children. The second goal was to find out if the dietary supplements with iodine (or any other combination of food rich on iodine) are used by observed individuals with ioduria over 300u/l. The results of research show if the respondent's level of ioduria is affected by in taking the dietary supplements or food with significantly higher content of iodine. My own research proved relatively frequent (but irregular) intake of dietary supplements with iodine by whole examined population. On the other hand, usage of dietary supplements with iodine is probably not correlated with usage of table salt without iodine or with thyroid gland illness by individual respondent or in his family. The milk and milk products and table salt with iodine proved to be the main source of iodine. According to research, children with excessive ioduria consume sources of iodine as frequent as other children. Deeper insight into results shows that reason for excessive saturation with iodine is combination of intake of dietary supplements as well as other significant food sources of iodine, especially milk and its products. By several respondents, the higher level of ioduria can be caused by higher consummation of sea products or by usage of dietary supplements with iodine in last two days. The need for better awareness about suitable rate of iodine dietary supplements intake as well as medical importance for lowering the amount of (iodine) table salt in food was discovered by research. The optimal supply of iodine by higher percent of observed population could be also achieved by optimizing the content of iodine in milk.
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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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Swarm Intelligence
Winklerová, Zdenka ; Šaloun, Petr (referee) ; Škrinárová,, Jarmila (referee) ; Zbořil, František (advisor)
The intention of the dissertation is the applied research of the collective ( group ) ( swarm ) intelligence . To demonstrate the applicability of the collective intelligence, the Particle Swarm Optimization ( PSO ) algorithm has been studied in which the problem of the collective intelligence is transferred to mathematical optimization in which the particle swarm searches for a global optimum within the defined problem space, and the searching is controlled according to the pre-defined objective function which represents the solved problem. A new search strategy has been designed and experimentally tested in which the particles continuously adjust their behaviour according to the characteristics of the problem space, and it has been experimentally discovered how the impact of the objective function representing a solved problem manifests itself in the behaviour of the particles. The results of the experiments with the proposed search strategy have been compared to the results of the experiments with the reference version of the PSO algorithm. Experiments have shown that the classical reference solution, where the only condition is a stable trajectory along which the particle moves in the problem space, and where the influence of a control objective function is ultimately eliminated, may fail, and that the dynamic stability of the trajectory of the particle itself is not an indicator of the searching ability nor the convergence of the algorithm to the true global solution of the solved problem. A search strategy solution has been proposed in which the PSO algorithm regulates its stability by continuous adjustment of the particles behaviour to the characteristics of the problem space. The proposed algorithm influenced the evolution of the searching of the problem space, so that the probability of the successful problem solution increased.
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Profile of visitors of the Toulava touristic region
PLZÁKOVÁ, Aneta
This bachelor thesis focuses on tourist area Toulava which arose three years ago. It mentions the history of Toulava which was created as a combination of beautiful nature and interesting tourist sites. It analyses what visitors always do here and popular tourist sites. The first part focuses on literature. The practical part of the work introduces two researches which were done during the summer season in 2013 and through the summer in 2015. The goal was to get a general visitor profile of Toulava. Tourist from this region filled in the questionnaires relating to this area, which were placed at various tourist spots. The work concludes that the visitors profile is still the same and tries to suggest a new product for gaining the interest of potential tourist.
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