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Determination of sweeteners and preservatives in energy drinks by HPLC
Zídková, Anežka ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
This master´s thesis is focused on simultaneous determination of sweeteners and preservatives in energy drinks by liquid chromatography coupled with DAD and ELSD detection. The method was optimized for determination of aspartame, acesulfame K, saccharin, sucralose, steviol glycosides, benzoic acid and sorbic acid. Analyses were carried out on the Poroshell 120 EC-C18 column (4.6 x 150 mm, 2.7 m, Agilent) using mixture of methanol, acetone and water with formic acid and trimethylamine as a gradient mobile phase at a flow rate 0,5 mL•min-1. Validation parameters were determined (limit of detection, limit of quantification, repeatability and recovery). The validated method was applied on real samples.
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Potential microbial hazards in actual cosmetology trends - bio, vegan, raw
Jančíková, Simona ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Bio-, vegan- and raw-cosmetics are continually more popular type of cosmetic products caused by an absence of synthetic and preservative substances but at the same time there is an increased probability of microbial contamination. The main aim of this thesis is a practical findings of potential microbiological contamination using a challenge test and a determination of any active ingredients in the cosmetic preparations. The legislation of cosmetic preparations as well as the information about certificates, which are awarded for bio-, vegan- and raw-cosmetics has been processed in the theory. This section also discusses a composition of vegetable and essential oils, which are used as raw materials in cosmetics production to a great extent and have a positive effect on skin. This thesis also deals with a possible microbiological contamination, listing the most frequently occurred pathogenic microorganisms, which can have a negative influence on consumer health. Operating life of selected samples using the Schülke Koko test by inoculating selected representatives of bacteria, yeasts and molds (Candida glabrata, Escherichia coli, Pseudomonas aeruginosa and Aspergillus niger) are being tested in the practical part. In addition a possible antimicrobial activity which may partially replace an effect of synthetic preservatives in the presence of essential oils is also being tested. Finally any biological active substances were analysed by the gas chromatography, in particular fatty acids contained in the creams.
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Determination of propionic acid in bakery products by liquid chromatography
Benkovská, Dagmar ; Vítová, Eva (referee) ; Kučera, Pavel (advisor)
This thesis deals with the development of high performance liquid chromatography method (HPLC) for the determination of propionic acid in bakery products. This work is resulting from the requirements and conditions of Czech Agriculture and Food Inspection Authority (CAFIA) laboratory in Brno. The theoretical part of the thesis describes properties, production, metabolism, toxicity and preservative effect of propionic acid. Furthermore it is treating about HPLC, validation of some analytical method and methods of propionic acid determination. Experimental part is focused on the propionic acid determination in the major part of available products with this preservative on the Czech market. Propionic acid was extracted from the sample with water, filtered and the filtrate was analyzed directly by HPLC. Separation was performed on a XBridge C18 column (3 x 150 mm, 3,5 µm) with a mobile phase of phosphate buffer (pH adjusted to 2,8) and UV detection at 210 nm. Used method of propionic acid isolation was compared with repeated extraction method, extraction in the mobile phase solution and steam destilation both in commercial samples and in laboratory baked breads with known addition of propionic acid. HPLC with water extraction technique was evaluated as a suitable method for propionic acid determination in bakery products.
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Effect of preservatives on the sensory properties of ham
Bělohoubková, Tereza ; Hudečková, Helena (referee) ; Mikulíková, Renata (advisor)
The Master’s thesis contemplates the effect of additives on sensory properties of ham. The theoretical part incorporates history of meat product preservation and characterization of ham from legislative and compositional point of view. Technological ham making process in the Czech republic as well as worldwide and nutritional value of cooked ham are described. Further on the thesis contains a description of traditional and new sensory evaluation methods. Discussion of current solution of the issue concludes the theoretical part. In the practical part technological highest standard ham making process was optimalized, without the use of industrial manufacturing devices. Samples of ham containing nitrite salting mix and sodium nitrate along with a ham sample without the additon of preservatives were prepared. Total content of moisture, proteins, ash and fats was determined in these samples by regular standardized methods, that were statistically compared by paired test or analysis of variance (ANOVA) with results obtained by FoodScanTM instrument. Fatty acids content was determined by gas chromatography with FID detector in samples containing additives and a sample without additives. Effect of additive adittion in ham on nutritional characteristics, fatty acids content and on concentration of TBARS (lipid oxidation products) was investigated. Within sensory analysis effect of additives on sensory properties of ham was studied by CATA method. Sensory panel consisted of consumers. In the first sensory analysis of ham samples with addition of preservatives (nitrite salting mix, sodium nitrate, sodium acetate and sodium lactate) consumers picked ham containing nitrite salting mix as the most acceptable. The chosen sample served as a base for the second sensory analysis of samples containing antioxidants (sodium erythorbate, butylhydroxyanisole, butylhydroxytoluene, sodium ascorbate) and stabilizers (sodium hydrogen phosphate and potassium phosphate). In the second sensory analysis the consumers had difficulty distinguishing the samples. Part of the sensory analysis was a marketing research and a study of ham additives perception from a consumer’s point of view.
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Quality of selected meat products produced in standard and organic quality
JAROŠOVÁ, Martina
The aim of this Master thesis was to compare the presence of used preservation substances and basic ingredients in meat products made in regular and bio-quality. In practical part of the thesis, a polarization measurement was done with the aim to determine whether there is a quality difference between these two production qualities. The obtained data were subsequently processed and statistically evaluated. The statistical evaluation showed that the basic composition of meat products in regular and bio-quality is basically identical in percentage. Furthermore, the frequency of used preservation additives in different foods was statistically evaluated as well. In conclusion, food products in bio-quality showed 0.7 less additive content compared to regular food product, however, the difference was still weighed as statistically insignificant.
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Determination of sweeteners and preservatives in energy drinks by HPLC
Zídková, Anežka ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
This master´s thesis is focused on simultaneous determination of sweeteners and preservatives in energy drinks by liquid chromatography coupled with DAD and ELSD detection. The method was optimized for determination of aspartame, acesulfame K, saccharin, sucralose, steviol glycosides, benzoic acid and sorbic acid. Analyses were carried out on the Poroshell 120 EC-C18 column (4.6 x 150 mm, 2.7 m, Agilent) using mixture of methanol, acetone and water with formic acid and trimethylamine as a gradient mobile phase at a flow rate 0,5 mL•min-1. Validation parameters were determined (limit of detection, limit of quantification, repeatability and recovery). The validated method was applied on real samples.
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Potential microbial hazards in actual cosmetology trends - bio, vegan, raw
Jančíková, Simona ; Skoumalová, Petra (referee) ; Němcová, Andrea (advisor)
Bio-, vegan- and raw-cosmetics are continually more popular type of cosmetic products caused by an absence of synthetic and preservative substances but at the same time there is an increased probability of microbial contamination. The main aim of this thesis is a practical findings of potential microbiological contamination using a challenge test and a determination of any active ingredients in the cosmetic preparations. The legislation of cosmetic preparations as well as the information about certificates, which are awarded for bio-, vegan- and raw-cosmetics has been processed in the theory. This section also discusses a composition of vegetable and essential oils, which are used as raw materials in cosmetics production to a great extent and have a positive effect on skin. This thesis also deals with a possible microbiological contamination, listing the most frequently occurred pathogenic microorganisms, which can have a negative influence on consumer health. Operating life of selected samples using the Schülke Koko test by inoculating selected representatives of bacteria, yeasts and molds (Candida glabrata, Escherichia coli, Pseudomonas aeruginosa and Aspergillus niger) are being tested in the practical part. In addition a possible antimicrobial activity which may partially replace an effect of synthetic preservatives in the presence of essential oils is also being tested. Finally any biological active substances were analysed by the gas chromatography, in particular fatty acids contained in the creams.
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Determination of propionic acid in bakery products by liquid chromatography
Benkovská, Dagmar ; Vítová, Eva (referee) ; Kučera, Pavel (advisor)
This thesis deals with the development of high performance liquid chromatography method (HPLC) for the determination of propionic acid in bakery products. This work is resulting from the requirements and conditions of Czech Agriculture and Food Inspection Authority (CAFIA) laboratory in Brno. The theoretical part of the thesis describes properties, production, metabolism, toxicity and preservative effect of propionic acid. Furthermore it is treating about HPLC, validation of some analytical method and methods of propionic acid determination. Experimental part is focused on the propionic acid determination in the major part of available products with this preservative on the Czech market. Propionic acid was extracted from the sample with water, filtered and the filtrate was analyzed directly by HPLC. Separation was performed on a XBridge C18 column (3 x 150 mm, 3,5 µm) with a mobile phase of phosphate buffer (pH adjusted to 2,8) and UV detection at 210 nm. Used method of propionic acid isolation was compared with repeated extraction method, extraction in the mobile phase solution and steam destilation both in commercial samples and in laboratory baked breads with known addition of propionic acid. HPLC with water extraction technique was evaluated as a suitable method for propionic acid determination in bakery products.
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Use of conservation technologies in meat proccesins and produktion of meat products
KUBECOVÁ, Dagmar
This thesis deals with the use of conservation technologies in meat processing and production of meat products. The aim is to gather available information on methods to extend the life of the issue of meat and meat products. In her first retrieval character are generally mentioned meanings conservation technologies and described the principles of conservation, protection and legal requirements associated with it. It further describes the various methods of preserving meat and meat products and their problems including the latest ways that are not yet instance for their high financial demands of commercially exploited. Methods for evaluating the effect of reducing or fully suppressing the activity of microbes. Following the issue of limiting the processing technology and last but not least, there is mention of Additives meat and meat products, antimicrobial substances and antioxidants. Furthermore, there are new trends in contemporary conservation and the conclusion is dedicated to the packaging of meat and meat products, which are monitored by means of long-term storage. The end is explained the principle of vacuum packaging.
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