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Study of genetic factors modifying the risk of onset and progression of colorectal and pancreatic cancer
Mohelníková Duchoňová, Beatrice ; Souček, Pavel (advisor) ; Skálová, Lenka (referee) ; Macek, Milan (referee)
Introduction: The aim of this study was to evaluate the role of genetic and lifestyle factors in the risk of onset and progression of colorectal and pancreatic cancer. The first part deals with the etiological factors and the importance of polymorphisms in biotransformation enzymes and genetic alterations in the gene CHEK2 in the origin of these malignancies. In the second part, the ABC transporter genes were analyzed as potential prognostic and predictive markers of a treatment's outcome. Materials and methods: The polymorphisms and other genetic alterations were detected using real-time PCR, allelespecific PCR and PCR-RFLP methods in DNA which was extracted from the blood of patients. The frequency of polymorphisms was evaluated and their importance was assessed with regard to the available epidemiological data. Gene expressions were determined by qPCR in paired samples of tumor tissue and adjacent non-tumorous parenchyma. Results: A majority of the observed polymorphisms failed to show a relationship between their presence and the risk of any of these malignancies. CYP2A13 variant allele*7 coding inactive enzyme was found in 7 of 265 controls and in none of 235 pancreatic carcinoma patients. In contrast, GSTP1-codon 105 Val variant allele and GSTT1-null genotype were associated with an elevated...
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The use of physiological and pathophysiological pressure ratios in the area of the biliary ductal system and pancreas for diagnosis and treatment by endoscopic retrograde cholangiopancreatography
Keil, Radan ; Kvapil, Milan (advisor) ; Frič, Přemysl (referee) ; Hep, Aleš (referee) ; Mareš, Jan (referee)
:.In our work we wanted to confirm our clinical experience with therapy of biliary and pancreatic duct injuries from the endoscopic retrograde cholangiopancreatography (ERCP) which was done in 267. Children and infants with a variety of biliary tract disorders and traumatic injuries in the area of biliary and pancreatic duct.. Pressure of the bile plays the key role in the therapy of biliary tract injuries Therefore we have measured the pressure in biliary tract and duodenum before and after the sphincterotomy of Oddi sphincter. Thea aim of our study was to confirm the insertion of drainage into the biliary and pancreatic duct in children with injury in this area. Our results showed significant differences between biliary duct pressure and duodenal pressure in the patients before and after sphincterotomy of Oddi sphincter. This results on theoretical basis confirmed, that it is necessary in children after traumatic rupture of biliary duct to provide ERCP and insert a biliary drainage after sphincterotomy. With this procedure the biliary tract injury is healed ad integrum without surgical liver resection. To provide only papilosphincterotomy without biliary drainage is not sufficient. This new miniinvasive procedure plays a fundamental role in the therapy of blunt abdominal injuries in a children and infants...
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Dietární opatření při chronické pankreatitidě
Pavková, Lucie
Chronic pancreatitis (CHP), along with the acute form of the inflammatory diseases of the pancreas. While acute pancreatitis (AP) is really a matter with which one can meet only once in your life, mortality is still 25-30 %. Unlike acute pancreatitis, a chronic disease of this type is defined as a condition in which there is a permanent loss of external secretory function of the pancreas replacing the function of the pancreatic parenchyma tissue. I have chosen the CHP directly to pointed out the seriousness of this disease that affects more and more people and attention to external and internal factors that this disease can both positively and negatively influenced. There is diet as an important factor. That includes strict dietary measures which are necessary for patients with this disease. I explore the possibilities of diet and construct a suitable diet for each stage of the disease with the help of nutritional therapist.
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