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Extraction and application of keratin from sheep wool
Jahnová, Kateřina ; Veselá, Mária (referee) ; Kovalčík, Adriána (advisor)
This thesis deals with the isolation of sheep wool keratin for using this protein in composite films. The goal was to compare the yield of alkaline hydrolysis of sheep wool using different concentrations of sodium hydroxide. It was found that the most effective was alkaline hydrolysis using 2wt% sodium hydroxide showing 80 % effectiveness of keratin isolation. Fourier infrared spectroscopy, differential scanning calorimetry a thermo gravimetric analyses confirmed that the isolated materiál from sheep wool was keratin. The isolated keratin was used as filler for the preparation of polyvinyl alcohol-based composite films. The mechanical properties of keratin/polyvinyl alcohol films showed that keratin played the role as an active filler for polyvinyl alcohol composites.
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Vliv potravní preference všenek (Phthiraptera: Amblycera) na formování jejich mikrobiomů
ŽIŽKOVÁ, Kateřina
Microbiome diversity of chewing lice belongs to generaly unexplored topics. This thesis provides the first insight into the microbiomes of 15 chewing lice species of the genus Menacanthus. We detected the presence of two dominant symbiotic bacteria, which occurred in almost all species. We also compared the microbiome diversity of lice with different diet preferences (blood feeding and feather feeding species). Based on these analyses, we identified the main determinants shaping the microbiomes of Menacanthus lice. The microbiomes are namely affected by Menacanthus species identity, its geographical origin, dietary niche, and the extent of the host spectrum that the lice parasitize.
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Diversity and evolutionary significance of feather-degrading bacteria in birds
Kyptová, Markéta ; Javůrková, Veronika (advisor) ; Reif, Jiří (referee)
Although to date this fact has been widely neglected, avian plumage is inhabited by a wide range of bacteria able to utilize keratin, the basic structural unit of feathers. If these keratinophilic, so called feather-degrading bacteria (hereafter FDB) get out of control, it can lead to significant changes in the plumage structural properties, resulting in impaired thermoregulation, flight performance, and expression of plumage ornaments. These may have significant consequences on reproductive success and overall fitness of individual. Based on these facts, it is hypothesized that PDB pose strong selective pressure lying behind the evolution of defense mechanisms such as size and chemical composition of uropygial gland, deposition of pigments into the feathers, or molting strategies that have been observed to eliminate the negative effect of FDB. The aims of this bachelor thesis are, on the basis of available literature, to summarize findings related to overall diversity and characteristics of plumage bacteria, to evaluate their effect on quality of plumage and individual fitness, and also to assess a role of microorganism in the evolution of different life- strategies in birds. Finally, this bachelor thesis points out the shortcomings of recent studies and proposes the concept for future research...
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In-vivo and ex-vivo models for superficial mycosis
Míková, Ivana ; Hubka, Vít (advisor) ; Julák, Jaroslav (referee)
12. 5. 2016 In vivo a ex vivo modely povrchových mykóz (bakalářská práce) Ivana Míková Abstract In my bachelor thesis I have dealt with the importance of using the laboratory animals (in vivo models) and artificial tissue (ex vivo models) for studying the superficial mycosis. The purpose of using these models is especially to clarify the pathogenesis of illness (the pathogenʼs penetration into the organism, the clinical, histopathological and immunological changes) or testing of new treatments. Selection of a suitable and susceptible model, methodics of preparation of the inoculum and preparation of the model before inoculation are crucial for the successful creation of the experiment. Small rodents (guinea-pig, mouse, rat), skin equivalents and keratin films made from human hair, nails and animal hooves are the most used in vivo and ex vivo models. This bachelor thesis summarizes their advantages and disadvantages. Furthermore, the work deals with the types of dermatophytes and thermally dimorphic fungi used for successful inoculation and with the specific purpose of their use. The dermatophytes (Trichophyton, Microsporum, Epidermophyton, Arthroderma) and thermally dimorphic fungi (Blastomyces and Sporothrix) are the most often researched originators of the superficial mycosis. Both groups cause the animal...
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Changes in the mechanical parameters of women's hair during pregnancy
Skřontová, Marie ; Jelen, Karel (advisor) ; Tlapáková, Eva (referee) ; Berchová, Kateřina (referee)
Title: Changes in the mechanical parameters of women's hair during pregnancy Matters: We can look from different angles on the hair - as on a nanocomposite fiber and as on biomaterial changing with the origin and age. The hair doesn't differ only by lenght, stucture and color but also by diameter and shape. It reflects the overall health of the individual and all the processes in the organism of the individual and thus also the pregnancy. During pregnancy, hormonal changes take place which have an effect on the hair. Many women experience faster hair growth, extension and increased volume of the hair during pregnancy. This status is only temporary and lasts only to the childbirth. Aim: The aim of this work was to show the influence of pregnancy on mechanical parameters of hair and what direction this influence takes. Next, using questionnaires, to better solve the effect of particular pregnancy parameters on the hair, i.e. pregnancy order, sex of the child. Then, evaluate the whole problem using statistical tests and so make better sense of it. Methods: We'd selected a group of 64 pregnant women; hair samples were cut from them in the nape area each month throughout pregnancy. Each measurement started with evaluation of hair diameter with the use of optical microscope. Next, the hair had been...
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Changes in the mechanical parameters of women's hair during pregnancy
Skřontová, Marie ; Jelen, Karel (advisor) ; Tlapáková, Eva (referee) ; Berchová, Kateřina (referee)
Title: Changes in the mechanical parameters of women's hair during pregnancy Matters: We can look from different angles on the hair - as on a nanocomposite fiber and as on biomaterial changing with the origin and age. The hair doesn't differ only by lenght, stucture and color but also by diameter and shape. It reflects the overall health of the individual and all the processes in the organism of the individual and thus also the pregnancy. During pregnancy, hormonal changes take place which have an effect on the hair. Many women experience faster hair growth, extension and increased volume of the hair during pregnancy. This status is only temporary and lasts only to the childbirth. Aim: The aim of this work was to show the influence of pregnancy on mechanical parameters of hair and what direction this influence takes. Next, using questionnaires, to better solve the effect of particular pregnancy parameters on the hair, i.e. pregnancy order, sex of the child. Then, evaluate the whole problem using statistical tests and so make better sense of it. Methods: We'd selected a group of 64 pregnant women; hair samples were cut from them in the nape area each month throughout pregnancy. Each measurement started with evaluation of hair diameter with the use of optical microscope. Next, the hair had been...
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In-vivo and ex-vivo models for superficial mycosis
Míková, Ivana ; Hubka, Vít (advisor) ; Julák, Jaroslav (referee)
12. 5. 2016 In vivo a ex vivo modely povrchových mykóz (bakalářská práce) Ivana Míková Abstract In my bachelor thesis I have dealt with the importance of using the laboratory animals (in vivo models) and artificial tissue (ex vivo models) for studying the superficial mycosis. The purpose of using these models is especially to clarify the pathogenesis of illness (the pathogenʼs penetration into the organism, the clinical, histopathological and immunological changes) or testing of new treatments. Selection of a suitable and susceptible model, methodics of preparation of the inoculum and preparation of the model before inoculation are crucial for the successful creation of the experiment. Small rodents (guinea-pig, mouse, rat), skin equivalents and keratin films made from human hair, nails and animal hooves are the most used in vivo and ex vivo models. This bachelor thesis summarizes their advantages and disadvantages. Furthermore, the work deals with the types of dermatophytes and thermally dimorphic fungi used for successful inoculation and with the specific purpose of their use. The dermatophytes (Trichophyton, Microsporum, Epidermophyton, Arthroderma) and thermally dimorphic fungi (Blastomyces and Sporothrix) are the most often researched originators of the superficial mycosis. Both groups cause the animal...
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