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Pretreatment of polysaccharide waste materials to maximize of hydrolysis products
Myslivcová, Pavla ; Trachtová, Štěpánka (referee) ; Babák, Libor (advisor)
This thesis deals with study of the polysacharidic waste material pretreatment in order to maximize the hydrolysis products. It has been chosen a pastry as a waste material, specifically rolls and bread. Pastry was pretreated by milling and 15% w/v suspension of pastry in the water was prepared. Then several types of hydrolysis were performed with suspensions. Products of hydrolysis were characterized by spectrophotometric method of Somogyi-Nelson and efficiency of hydrolysis was compared. Three acids (H2SO4, HNO3, HCl) and two alkalis (NaOH, KOH) in three various concentration were used for the chemical hydrolysis. Microwave and ultrasound were chosen for the physical hydrolysis.
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Application of Zymomonas mobilis for bioethanol production from cellulose materials
Čalová, Iveta ; Zichová, Miroslava (referee) ; Babák, Libor (advisor)
The thesis deals with the use of Zymomonas mobilis to produce bioethanol from waste paper. There were used three types of paper made by various manufacturers to optimize the production of bioethanol. Individual papers were subjected to the same adjustments namely mechanical grinding and subsequent enzymatic hydrolysis. During cultivation of Zymomonas mobilis in different hydrolysates were taken samples for determination of bioethanol production. The analysis was carried out on high-performance liquid chromatography. Production of bioethanol and glucose consumption was evaluated as well as production of lactic acid since it was also noticeable on the chromatograms.
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Hydrolysis of potatoes
VLAČIHOVÁ, Andrea
This work compares acid, alkaline and enzymatic hydrolysis of potato starch with simultaneous application of various pressures (0MPa, 0,3MPa and 1MPa). Acid hydrolysis was made by sulphuric acid of intensity 3, 6, 9 and 12 %, alkaline hydrolysis was made by sodium hydroxide of intensity 3, 6, 9 and 12 % and four enzymatic preparations for starch hydrolysis were used for enzymatic hydrolysis. Hydrolysis effect was measured via GE and added by alcohol yield. There was positive influence of increased pressure 0,3MPa and 1Mpa on hydrolysis process visible in this experiment.
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Hydrolysis of corn silage
MÍKA, Václav
Aim work is compare yield GE at acid, alkalic and enzymatic hydrolysis cellulose phytomass at different acidity (H2SO4, 3, 6, 9 and 12%), fundamentals (NaOH, 3, 6, 9 and 12%) and while using different enzymatic preparations (6 preparations to hydrolysis celluloses and hemicelluloses). Further is assessed positive influence compressive extrusion (0,3MPa and 1MPa).
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Petelyza-Chemical PET Recycling
Veselý, Václav
Chemical recycling of PET is carried through alkaline hydrolysis. The produkt is salt of terephtalic acid, which is electrolytically furthet separated into PTA and alkaline glykol solution. Vakuum destilation is applied to get etylenglycol.
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