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Diversity of European freshwater cyclopoid species: phylogeny, morphology and ecology
Krajíček, Martin ; Černý, Martin (advisor) ; Brancelj, Anton (referee) ; Wyngaard, Grace (referee)
Cyclopoids are together with Calanoids and Harpacticoids a part of the largest, very diverse group of crustaceans and the most numerous aquatic metazoans of the world. The history of their research goes as far back as to the beginning of 19th century when the first cyclopoid copepods were described. The taxonomy of cyclopoids started to develop gradually since that time, adding new and more detailed methods and morphological characters, as well as a certain degree of taxonomical confusion. In last decades, the molecular-genetic techniques of DNA sequencing have become available offering a new independent tool for taxonomists. This work contains different studies concerning the morphology, taxonomy, ecology, distribution and colonisation of cyclopoid copepods, with the use of molecular tools as a uniting element. Chapter 1 of this thesis summarizes basic knowledge about the taxonomy, morphology and biology of cyclopoid copepods and introduces the following chapters containing four studies presented as single publications. The taxonomy of copepods of the genus Cyclops is based mainly on the morphology which is sometimes ambivalent and some of the most problematic species groups are presented here. Chapter 2 presents our unique results, the first reconstruction of phylogenetic relationships among 15...
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Phylogeography and population structure of two loach species (Teleostei: Nemacheilidae) in Southeast Asia
Dvořák, Tomáš ; Bohlen, Jörg (advisor) ; Říčan, Oldřich (referee)
The freshwater fish species Schistura robertsi and Paracanthocobitis zonalternans belong to the family Nemacheilidae, which is distributed across whole Eurasia and with one species in northeast Africa. P. zonalternans occurs in lowland habitats through western Southeast Asia from Central Myanmar until northern Malaysia. The distribution area is of biogeographic interest, because it crosses several known biogeographic barriers, namely the border between Indian and Indochinese freshwater fauna along the Salween River, the Isthmus of Kra, the Krabi - Surat Thani line and the Kangar - Pattani line. In the present study, around 250 specimens of P. zonalternans from 62 localities across the whole distribution area were investigated using genetic (nuclear and mitochondrial sequences), morphologic and geologic data. The genetic data reveal the existence of seven major clades within the analysed material, each of them with a distinct geographic distribution area and only few cases of overlap, but with occurrence of some cases of secondary contact. Divergence time estimations suggested that P. zonalternans is about 18 my old, and a biogeographic analysis located the region of origin in the Tenasserim region (nowadays southern Myanmar). The global sea level fluctuations seem to have had a strong impact on the...
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Microbiota as a modulator of carcinogenesis
Benešová, Iva ; Kverka, Miloslav (advisor) ; Krulová, Magdaléna (referee)
Many studies show the ability of gut microbes to modulate the anti-tumour immune response by direct triggering the immune cells or by bacterial metabolites. Interestingly bacteria may even migrate to the tumour tissue and orchestrate the immune response on site. These anti-tumour effects can be improved by the administration of immune checkpoint inhibitors (ICI). Notably, some microbial effects occur only in the presence of ICI. On the contrary, microbiota may also promote tumour growth and negatively impact the effects of ICI therapy. We have disrupted the gut microbiota homeostasis by antibiotics (ATB) to study the effects of gut microbiota on the ICI. This disturbance led surprisingly to reduced tumour growth and enhanced pro-inflammatory immune response not only in the gut but also within the tumour tissue, where especially IFN-γ orchestrated the anti-tumour immune response. Importantly the anti-tumour immune response could be transferred through colonisation of germ-free mice by ATB-changed gut microbiota if concomitantly anti- programmed cell death protein 1 (αPD-1) monoclonal antibody was administrated. These mice had elevated levels of segmented filamentous bacteria (SFB), which induced systemic immune response with increased expression of IL-17 and elevated amounts of Th 17 cells,...
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Diversity of European freshwater cyclopoid species: phylogeny, morphology and ecology
Krajíček, Martin
Cyclopoids are together with Calanoids and Harpacticoids a part of the largest, very diverse group of crustaceans and the most numerous aquatic metazoans of the world. The history of their research goes as far back as to the beginning of 19th century when the first cyclopoid copepods were described. The taxonomy of cyclopoids started to develop gradually since that time, adding new and more detailed methods and morphological characters, as well as a certain degree of taxonomical confusion. In last decades, the molecular-genetic techniques of DNA sequencing have become available offering a new independent tool for taxonomists. This work contains different studies concerning the morphology, taxonomy, ecology, distribution and colonisation of cyclopoid copepods, with the use of molecular tools as a uniting element. Chapter 1 of this thesis summarizes basic knowledge about the taxonomy, morphology and biology of cyclopoid copepods and introduces the following chapters containing four studies presented as single publications. The taxonomy of copepods of the genus Cyclops is based mainly on the morphology which is sometimes ambivalent and some of the most problematic species groups are presented here. Chapter 2 presents our unique results, the first reconstruction of phylogenetic relationships among 15...
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Species traits and ecological conditions linked to bird colonisation of cities
Grünwald, Jan ; Reif, Jiří (advisor) ; Sedláček, Ondřej (referee)
Human settlements are a novel habitat type and species must respond to its ongoing expansion. For effective conservation of biodiversity, it is important to understand the mechanisms underlying these responses. This thesis focuses on urbanization in birds, from both the individual and community perspectives. The aim of this thesis is to find out if there are any "pre-adaptations" in some bird species to be successful city dwellers or if all birds can potentially exploit the urban environment when local conditions allow. According to the reviewed literature, I conclude that the urbanization in birds is not a universal pattern, but there are some specific traits shared by most of the urban birds. The successful urban species are mostly omnivorous or granivorous, they breed in trees or in cavities and, they are often ecological generalists. They are sometimes represented by non-native species occurring in the cities all over the world.
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The image of slave in Patrick Chamoiseau's Creol tales.
Svobodová, Kateřina ; Fučíková, Milena (advisor) ; Šarše, Vojtěch (referee)
v anglickém jazyce The subject of this diploma thesis is the work of the francophone writer Patrick Chamoiseau. The thesis reflects especially the theme of slavery in short stories and fairy tales of this Martinique author. The diploma thesis puts Patrick Chamoiseau's work in a historical and political context as well as the personal life of the writer. The diploma thesis describes geographic and cultural particularities of the Lesser Antilles and analyzes in more detail the story The Slave Old Man and the Giant Dog and selected fairy tales. Based on this analysis, the thesis describes typical themes and language tools used by Patrick Chamoiseau. The thesis also deals with oral literature and culture of Martinique and the current problems of society are also discussed through a reflection of another work by Patrick Chamoiseuau.
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Phylogeography and population structure of two loach species (Teleostei: Nemacheilidae) in Southeast Asia
Dvořák, Tomáš ; Bohlen, Jörg (advisor) ; Říčan, Oldřich (referee)
The freshwater fish species Schistura robertsi and Paracanthocobitis zonalternans belong to the family Nemacheilidae, which is distributed across whole Eurasia and with one species in northeast Africa. P. zonalternans occurs in lowland habitats through western Southeast Asia from Central Myanmar until northern Malaysia. The distribution area is of biogeographic interest, because it crosses several known biogeographic barriers, namely the border between Indian and Indochinese freshwater fauna along the Salween River, the Isthmus of Kra, the Krabi - Surat Thani line and the Kangar - Pattani line. In the present study, around 250 specimens of P. zonalternans from 62 localities across the whole distribution area were investigated using genetic (nuclear and mitochondrial sequences), morphologic and geologic data. The genetic data reveal the existence of seven major clades within the analysed material, each of them with a distinct geographic distribution area and only few cases of overlap, but with occurrence of some cases of secondary contact. Divergence time estimations suggested that P. zonalternans is about 18 my old, and a biogeographic analysis located the region of origin in the Tenasserim region (nowadays southern Myanmar). The global sea level fluctuations seem to have had a strong impact on the...
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Diversity of European freshwater cyclopoid species: phylogeny, morphology and ecology
Krajíček, Martin
Cyclopoids are together with Calanoids and Harpacticoids a part of the largest, very diverse group of crustaceans and the most numerous aquatic metazoans of the world. The history of their research goes as far back as to the beginning of 19th century when the first cyclopoid copepods were described. The taxonomy of cyclopoids started to develop gradually since that time, adding new and more detailed methods and morphological characters, as well as a certain degree of taxonomical confusion. In last decades, the molecular-genetic techniques of DNA sequencing have become available offering a new independent tool for taxonomists. This work contains different studies concerning the morphology, taxonomy, ecology, distribution and colonisation of cyclopoid copepods, with the use of molecular tools as a uniting element. Chapter 1 of this thesis summarizes basic knowledge about the taxonomy, morphology and biology of cyclopoid copepods and introduces the following chapters containing four studies presented as single publications. The taxonomy of copepods of the genus Cyclops is based mainly on the morphology which is sometimes ambivalent and some of the most problematic species groups are presented here. Chapter 2 presents our unique results, the first reconstruction of phylogenetic relationships among 15...
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Methods for studying seed dispersal and its importance for colonization of new sites
Vlasta, Tomáš ; Knappová, Jana (advisor) ; Hemrová, Lucie (referee)
Study of seed dispersal is crucial for understanding of several processes in life of plants and landscape. Seed dispesal is very important for persistence of local populations in fragmented landscape, it plays an important role in succession, it helps to protect plant populations againts loss of genetic variability. We usually recognize several types of seed dispersal depending on vector (dispersal by wind, animals and water.) In nature, it is very common that seed can be independently dispersed by several vectors. Therefore, studying of polychory and secondary dispersal is necessery. Methods of studying of seed dispersal are very variable, including seed traps, releasing and following individual seeds, genetics marcers etc. If we study seed dispersal, we should select the most suitable method depending on the aim of the study. We should also take into acount advantages and disadvantages of single method.
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Diversity of European freshwater cyclopoid species: phylogeny, morphology and ecology
Krajíček, Martin ; Černý, Martin (advisor) ; Brancelj, Anton (referee) ; Wyngaard, Grace (referee)
Cyclopoids are together with Calanoids and Harpacticoids a part of the largest, very diverse group of crustaceans and the most numerous aquatic metazoans of the world. The history of their research goes as far back as to the beginning of 19th century when the first cyclopoid copepods were described. The taxonomy of cyclopoids started to develop gradually since that time, adding new and more detailed methods and morphological characters, as well as a certain degree of taxonomical confusion. In last decades, the molecular-genetic techniques of DNA sequencing have become available offering a new independent tool for taxonomists. This work contains different studies concerning the morphology, taxonomy, ecology, distribution and colonisation of cyclopoid copepods, with the use of molecular tools as a uniting element. Chapter 1 of this thesis summarizes basic knowledge about the taxonomy, morphology and biology of cyclopoid copepods and introduces the following chapters containing four studies presented as single publications. The taxonomy of copepods of the genus Cyclops is based mainly on the morphology which is sometimes ambivalent and some of the most problematic species groups are presented here. Chapter 2 presents our unique results, the first reconstruction of phylogenetic relationships among 15...
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