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Methods of assesment and treatment of coeliac disease
Valkus, Martin ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
This diploma thesis deals with methods of determining and treatment of coeliac disease. In the theoretical part of the work sums up informations about coeliac disease - history, prevalence, etiopathogenesis, immunopathogenesis, possibilities of diagnosis, discusses about gluten-free diet, mentions legislation of the Czech Republic and the European Union and compares expensiveness of gluten-free diet and normal diet. The experimental part of this thesis compares PCR, ELISA and indirect immunofluorescence methods for assesment of determination of genetic predisposition and antibodies in coeliac disease (greatest emphasis was placed on the comparison of antibodies against gliadin and deamidated gliadin antibodies in IgA and IgG).
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Food intolerance
Jirkovská, Magdaléna ; Tlaskalová - Hogenová, Helena (advisor) ; Křížan, Jiří (referee) ; Seifertová, Eva (referee)
This bachelor's thesis is a literature research that deals with food intolerances. It focuses primarily on celiac sprue, describes the disease, its clinical manifestations, symptoms, complications, methods of investigation, diagnosis and treatment. Part of the thesis deals with relationship of gluten to other diseases such as gluten sensitivity or autism.
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Coeliac Disease and its Effect on Everyday Life
Hadravová, Petra ; Ehler, Edvard (advisor) ; Pavlasová, Lenka (referee)
Coeliac disease is an autoimmune digestive disease that damages the villi of the small intestine and interferes with absorption of nutrients from food, and which is among the most common disease of the immune system. Essentially the body is attacking itself every time a person with coeliac consumes gluten. Currently is coeliac untreatable, the only treatment is a lifelong gluten free diet. Low public awareness and frequent late diagnosis usually causes unnecessary discomfort. For this reason are the most important aims of my work define basic knowledges about coeliac disease, symptoms, diagnosis and treatment. At the same time it should also provide an information, especially to general public about the effect of the disease on the quality of life.
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Glaucoma - family-based genetic analysis in relation to autoimmunity
Buchtelová, Aneta ; Daňková, Pavlína (advisor) ; Ďuďáková, Ľubica (referee)
Introduction: Recent findings about the pathogenesis of glaucoma have already demonstrated the presence of some specific autoimmune mechanisms. It has also been shown that autoimmune diseases often manifest in co-occurrence, such as celiac disease and type 1 diabetes mellitus or psoriasis. This association can be explained by sharing some of the risk variants of HLA molecules class II. Considering glaucoma an autoimmune disease, the question raises how the glaucoma genetic risk factors affect the phenotype of another autoimmune disease or vice versa, whether genetic risk variants associated for example with celiac disease can affect the glaucoma phenotype. Aims: The aims of this study were to i) identify possible genetic risk markers associated with the development of glaucoma, based on the available literature, and to map their occurrence among members of a three-generation family suffering from glaucoma and multiple autoimmune diseases, ii) find carriers of HLA-DQ2/DQ8 among the members of the same family, iii) verify whether an individual's genotype correlates with his/her phenotype, and iv) determine the potential effect of specific HLA alleles on the glaucoma phenotype. Material and methods: This study used DNA samples derived from 34 members of a three-generation family, in which coeliac...
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Glaucoma - family-based genetic analysis in relation to autoimmunity
Buchtelová, Aneta ; Daňková, Pavlína (advisor) ; Ďuďáková, Ľubica (referee)
Introduction: Recent findings about the pathogenesis of glaucoma have already demonstrated the presence of some specific autoimmune mechanisms. It has also been shown that autoimmune diseases often manifest in co-occurrence, such as celiac disease and type 1 diabetes mellitus or psoriasis. This association can be explained by sharing some of the risk variants of HLA molecules class II. Considering glaucoma an autoimmune disease, the question raises how the glaucoma genetic risk factors affect the phenotype of another autoimmune disease or vice versa, whether genetic risk variants associated for example with celiac disease can affect the glaucoma phenotype. Aims: The aims of this study were to i) identify possible genetic risk markers associated with the development of glaucoma, based on the available literature, and to map their occurrence among members of a three-generation family suffering from glaucoma and multiple autoimmune diseases, ii) find carriers of HLA-DQ2/DQ8 among the members of the same family, iii) verify whether an individual's genotype correlates with his/her phenotype, and iv) determine the potential effect of specific HLA alleles on the glaucoma phenotype. Material and methods: This study used DNA samples derived from 34 members of a three-generation family, in which coeliac...
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Evaluation of functional properties of selected vegetable flours
VÍTKOVÁ, Věra
This bachelor thesis deals with basic structure, nutritional properties, production and use of flours. There are information about cereals and legumes, which are used for productions of flours. This thesis talks about food intolerances, which can be a reason why change basic flours of wheat, barley, oat and rye to another. Practical part is about 14 flours, which were tested on dry matter, humidity, nitrogen substances content, proteins, protein electrophoretic profiles (patterns), solubility, water and fat holding capacity, formation of gels and changes of colours during a boiling. The results of this research are information about nutrition and functional properties of tested flours.
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Nutrition for children suffering from coeliac disease
Heniková, Marina ; Nevoral, Jiří (advisor) ; Bronský, Jiří (referee)
Coeliac disease is a systemic autoimmune disease caused by gluten occurring in genetically susceptible individuals. Clinical manifestations of coeliac disease have changed over past decades. It became more diverse and begins later. Children with common gastrointestinal symptoms are on decline while children with uncommon extraintestinal symptoms are on the rise. The high sensitivity and specificity of coeliac- specific antibodies in a group of pediatric patients with disease manifestation allowed diagnosis without biopsy. The currently used treatment for coeliac disease is lifelong strict gluten-free diet. The aim of the work was to determine the current state of knowledge of coeliac disease in children, available diagnostical methods, and treatment of coeliac disease. Furthermore, my goal was to inform about the issue of gluten-free diet, the legislation, state support, contributions of health insurers, the range and availability of gluten-free foods, their financial demands and the function of patient organizations. The aim of the practical part of my work was to find out which circumstances triggered the initial coeliac disease diagnosis in children and to inform about the problems associated with maintaining a gluten-free diet in families with children suffering from coeliac disease. The select...
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Viruses in the pathogenesis of coeliac disease
Chudá, Kateřina ; Cinek, Ondřej (advisor) ; Šroller, Vojtěch (referee)
Celiac disease is a chronic inflammatory disorder affecting the small bowel. It develops in genetically susceptible individuals upon yet unknown environmental stimuli. Environmental triggers such as infections, dietary change or other "hits" are clearly required for disease development, as only a tiny fraction of genetically susceptible subjects develops celiac disease upon gluten exposure. This thesis aims to summarize the current evidence on viruses in the pathogenesis of celiac disease regarding their relevance in population or their involvement in immune processes leading to celiac disease. Rotavirus, orthoreovirus, adenovirus, astrovirus, respiratory syncytial virus, hepatitis viruses and herpesviruses are discussed. In addition, prospective cohort studies are presented that investigate environmental triggers of type 1 diabetes and celiac disease, two diseases sharing genetic predispositions. Keywords: celiac disease, orthoreovirus, rotavirus, adenovirus, astrovirus, respiratory syncytial virus, hepatitis C virus, hepatitis B virus, prospective cohort study
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Non-bioptic diagnosis of coeliac disease and following a gluten free diet
Jakešová, Andrea ; Szitanyi, Peter (advisor) ; Frühauf, Pavel (referee)
IN ENGLISH This thesis deals with non-biopsy diagnosis of coeliac disease and following the glu-ten-free diet in children. The theoretical part describes basic knowledge about coeliac dise-ase, its incidence, clinical presentation, diagnostics and prevention. This part also describes the principles of gluten- free diet, gluten-free labeling and financial demand of this diet.The research part deals with adherence to the gluten-free diet in pediatric patients both with bi-opsy and non-biopsy diagnosis. The aim of the study is also to determine if there are any differences between those two groups. The data were collected through the questionnaire that was mailed to selected respondents.Thirty eight respondents out of fifty responded to the study. The responses were recorded in a form of graphs. It was found out that 24 % of respondents knowingly violate gluten-free diet. No distinct difference of behaviour in both groups was recorded. 58 % of respondents considered the transition to a gluten-free diet as very challenging. The high costs of gluten-free food were recognized by respondents as the main problem. The results show that the majority of respondents were well informed about coeliac disease and the gluten-free diet. Education of newly diagnosed patients is very im-portant, because understanding...
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Diagnostics of the enzymatic defects in patients with coeliac disease
Bartoňová, Simona ; Herink, Josef (advisor) ; Semecký, Vladimír (referee)
Celiac sprue is a chronic, lifelong disease characterized by permanent intolerance to gluten. Small intestinal mucosa of these patients shows typical inflammatory changes. These changes lead to reduced absorption of nutrients, minerals and vitamins that may result malabsorption syndrome. Diagnosis of celiac disease must be a comprehensive evaluation of the results of immunological testing of autoantibodies and microscopic evaluation of the intestinal mucosa state. The bachelor thesis deals with the histochemical examination of intestinal biopsies, which allows better distinguish malabsorption syndromes of different origin. The aim of the study was to determine incidence of celiac sprue and evaluate the completeness of remission in patients on a gluten - free diet in patients aged over 50 years in intestinal biopsies samples taken in 2013. Strict gluten - free diet leads to reparation of mucosa and brush border enzyme activity. Biopsies were snap frozen immediately after collection, to preserve the morphology of tissue and the brush border enzyme activity. Reactions, demonstrating the enzymatic activity of the lactase, trehalase and dipeptidylpeptidase IV, were exercided on sections by previously described histochemical methods. Alteration of relief was assessed by Marsh score. In 2013 was recieved...
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