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Optimization of Hericium erinaceus Cultivation and Subsequent Fermentation with Probiotics
Radvanová, Tereza ; Vršanská, Martina (referee) ; Hudečková, Helena (advisor)
The focus of this diploma thesis is to optimize cultivation of the hericium erinaceus mushroom on cereal substrates with subsequent fermentation with probiotic bacteria. Theoretical part of this thesis focuses on description of the mushroom hericium erinaceus and its nutritional value, biological properties, other uses and cultivation. Furthermore, an overview of the nutritional value of the used cereal substrates is provided, along with the characterization of probiotics, their biological effects, and metabolism. Finally, a chapter dedicated to the description of the traditional koji fermentation process was included. In the experimental part, the cultivation of hericium erinaceus was optimized on seven cereal substrates – barley groats, cornmeal, wheat bran, buckwheat, amaranth, white and red quinoa. Using spectrophotometric methods, the content of water soluble proteins, total carbohydrates, total phenolic compounds, flavonoids, and antioxidant activity was determined in the cereal substrates themselves and substrates enriched with mycelium. Furthermore, the content of fatty acids was determined in the samples using the gass chromatography method and lipophilic compounds were analyzed using high performance liquid chromatography. Cereal substrates enriched with mycelium showed increased antioxidant activity and concentrations of water soluble proteins, phenolic compounds, flavonoids, and lipids. Moreover, elevated concentrations of tocopherols, ubiquinone, and ergosterol were detected through high performance liquid chromatography analysis. Substrates with abundant mycelial growth, such as barley groats, cornmeal, red quinoa, and amaranth, were selected for fermentation with probiotic bacteria Lactobacillus rhamnosus. After the fermentation process, spectrophotometric methods were repeated, and the concentration of lactic acid was determined using high performance liquid chromatography. Samples with probiotics showed a slight increase in their antioxidant activity, concentration of phenolic compounds, flavonoids, and water soluble proteins. The concentration of lactic acid in control samples and samples with probiotics was almost unchanged. The obtained results from this study suggest that cereals enriched with mycelium and the fermentation product of probiotics could be utilized in the development of dietary supplements or functional foods.
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Nové možnosti a technologie ve výrobě piva
Čechová, Kateřina
The bachelor thesis is focused on new ways of using raw materials in the brewing in-dustry and new innovations. The theoretical part mentions basic raw materials for beer production, special types of malt, solid adjuncts from other raw materials, especially pseudocereals, liquid adjuncts, various methods of processing hops and new proce-dures or new equipment in beer production. Also the principle of production of Czech beer and European beer is compared. In the second part, the possibility of using star-chy surrogates was investigated. A recipe was created using buckwheat and then the sensory profile of the beer was created. From the obtained results, it was found that the addition of buckwheat negatively affected the sensory properties of the beer, which was reflected in the negative evaluation of both aroma and taste.
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Development of fortified gluten-free cereals for specific nutrition
Dohnalová, Radka ; Fialová, Lenka (referee) ; Márová, Ivana (advisor)
The bachelor thesis was focused on the development of enriched gluten-free cereals for special nutrition. The fortified cereal product included processed microbial biomass, either as a freeze-dried powder or as an extract, for which the phenolic content, antioxidant content and protein content were determined. The theoretical part includes findings from professional literature on gluten-free cereals, pseudocereals and their chemical composition. Subsequently, a review on microalgae and yeasts was prepared including a list of their metabolites. The literature search focused on green microalgae and carotenogenic yeasts. In the experimental part, the characterization of the selected pseudocereals and optimization of the prepared extracts of the selected microalgae and carotenogenic yeast were firstly carried out. The selected pseudocereals were amaranth, buckwheat and quinoa. The green microalgae strain Chlamydomonas reinhardtii and the carotenogenic yeast strain Cystofilobasidium macerans were selected for enrichment of the pseudocereals. Carbohydrates, proteins, fatty acids, -glucans, gluten, vitamins, phenolic substances, flavonoids and antioxidants were determined in the pseudocereals. Total fatty acids, lipophilic substances, phenolic substances and total antioxidant activity were detected in the microbial biomass. Finally, mixed cereal products were prepared from pseudocereal flour, microbial biomass and microbial extract. The cytotoxicity of the selected extracts was determined by MTT assay. It was found that the addition of algal biomass alone or algal extract to cereal flours resulted in a significant increase in protein. The yeast ethanol extract increased the phenolic and antioxidant content of the cereal products.
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Effect of pollination on seedset and yield of buckwheat (\kur{Fagopyrum esculentum}).
NOLČ, Radek
Common buckwheat is a attracting plant for many pollinators, therefore it be helping to restore biodiversity in the landscape. The most important of pollinators of buc kwheat are honeybee and hoverflies. The aim of this thesis was to find out effect of pollination of buckwheat sown with honeybees and the others pollinators on the seed set and yield of archenes in different varieties of buckwheat. A small-plot field experiment was established with three varieties of common buckwheat in the Pilsen Region, in the place of Zábělá. Before flowering began, half of the plots were covered with insect screens. The buckwheat stand was evaluated from the point of: plants height, number of branches, number of flowers and inflorescences on a plant, nectar volume in Thrum flowers, flowering time, number of archenes on a plant, the weight of thousand of archenes and the yield of archenes. Monitoring of pollinators was performed at the time of buckwheat flowering at 9, 10, 11, 12 and 14 hours, during 10 minutes, using photos. Nectar collection took place on five consecu tive days, from 16. 7. until 20. 7., at 9, 10 and 11 hours. Total of 31 insect species were recorded on buckwheat flowers. The most common effective pollinators of buckwheat were honeybees, (39,7 %) and hoverflies (37,5 %). The significant difference in nectar production was found among the tested buckwheat varieties. The yield of achenes on covered plants was 87 % lower than on freely accessible plants.
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Evaluation of functional properties of selected vegetable flours
VÍTKOVÁ, Věra
This bachelor thesis deals with basic structure, nutritional properties, production and use of flours. There are information about cereals and legumes, which are used for productions of flours. This thesis talks about food intolerances, which can be a reason why change basic flours of wheat, barley, oat and rye to another. Practical part is about 14 flours, which were tested on dry matter, humidity, nitrogen substances content, proteins, protein electrophoretic profiles (patterns), solubility, water and fat holding capacity, formation of gels and changes of colours during a boiling. The results of this research are information about nutrition and functional properties of tested flours.
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Effect of various plasma sources on seed growth. Laboratory experimen in Buckwheat
Šerá, Božena ; Gajdová, Iveta ; Čermák, M. ; Gavril, B. ; Hnatiuc, E. ; Kováčik, D. ; Kříha, V. ; Sláma, J. ; Šerý, M. ; Špatenka, P.
Germination and early growth of buckwheat (Fagopyrum aesculentum) after low-temperature plasma discharge treatment in air gas generated in various apparatus were tested. Three pretreatment times were used: 180 s, 300 s and 600 s. Number of germinating achene, length and weight of sprouts were measured. The data was analysed with two-way ANOVA. A significant influence of the type of plasma apparatus, time of exposure and combination of both factors were found. The positive effect on germination and early growth was observed after application of GlidArc plasma. The opposite effect of PDBV apparatus, which is characterized by plasma with a high power density, was observed. Shorter pre-treatment times for this apparatus must be therefore used.
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Influence of genotype on rutin content in plants from genus \kur{(Fagopyrum)}
KRÁLOVÁ, Martina
The bachelor work is focused on evaluation of the influence of buckwheat species and variety on rutin containt in particular plant parts, on the total rutin yield per hectare production and on comparison varietal differences between both species, common buckwheat \kur{(Fagopyrum)}esculentum)} Moench) and tartary buckwheat \kur{(Fagopyrum tataricum)}. Rutin is a flavonol (quercetin-3-O- rutinosid) which is also called P vitamin. As well as it is known as the permeability factor or factor allowing permeability of the blood vessel. Rutin stimulates the C vitamin effect. Rutin has as well a high influence on hypertension, hypercholesterolemia and other cardiovascular diseases due to antioxidant properties. Buckwheat is the most important source of rutin. It is annual plant which belongs to Polygonaceae family and has a beneficial effect on human body. The common grown type is common buckwheat \kur{(Fagopyrum)}esculentum)} Moench) and less used type is buckwheat tartary \kur{(Fagopyrum tataricum)}. Tartary buckwheat \kur{(Fagopyrum tataricum)} contains several times higher amount of rutin than common buckwheat \kur{(Fagopyrum)}esculentum)} Moench). The highest amount of rutin is presented in the leaves and blossoms and the lowest one in seeds. Tartary buckwheat \kur{(Fagopyrum tataricum)} has much higher amount of rutin in seeds than common buckwheat \kur{(Fagopyrum)}esculentum)} Moench). The varietal differences in rutin content are considerable.
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