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Palm liveness detection and identification
Kala, Adam ; Goldmann, Tomáš (referee) ; Sakin, Martin (advisor)
This bachelor's thesis is focused on the detection of liveness of the human hand and its identification from multispectral images. The main goal of the work is the recognition of a living hand and its forgeries based on the information we have obtained, the finding of minutiae for palm identification and their use. The work describes techniques for cropping the area of interest, extracting characteristic features from the palm, and subsequent detection of liveness and identification of the person. Liveness detection success reached over 90% and person identification with a False Acceptance Rate (FAR) of less than 2% for selected datasets.
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Characterization of cytotoxic effect of combined antimicrobial nanomaterials
Kozlíčková, Hana ; Fialová, Lenka (referee) ; Márová, Ivana (advisor)
This thesis deals with the study of the effects of combined nanomaterials on human skin cells. Pure antimicrobial substances, two types of liposomes enriched with antimicrobial substances, nanofibers with antimicrobial substances and, finally, four types of combined nanomaterials were analyzed from the point of view of cytotoxicity. The analysed active substances were eugenol, thymol, cavarcrol, curcumin, vitamin E and the antibiotics streptomycin and ampicillin. In the theoretical part of the work, the cell line of human keratinocytes, used in the experimental part of the work for cell tests, was characterized. Furthermore, individual active substances with an antimicrobial effect were described and the principles of biological effects were described, which include antimicrobial, antioxidant, cytotoxic and synergistic effects. Additionally, the theoretical part described individual nanomaterials, their preparation and usage in cosmetics and medicine. The experimental part was based on the characterization of prepared nanomaterials and on testing the influence of individual antimicrobial substances on the proliferation and viability of human HaCaT cells. Using the DLS method, the size of the prepared liposomes was measured and the effect of PHB and the type of active substance on their size was studied. MTT and LDH tests were chosen to test the cytotoxicity of individual substances. Furthermore, a scratch test was performed to monitor the effect of the investigated substances on proliferation and the rate of wound healing by cells. The last performed tests were immune response assays, in which were tested the samples for production of the human anti-inflammatory cytokines IL-6 and IL-8. In experimental part of this thesis, it was found that the prepared nanofibrous materials are safe for use in healthcare or cosmetics and, in the future, suitable to produce nanofibrous wound coverings enriched with antimicrobial substances, which can give them exceptional properties.
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Skin resist and psycho-galvanic reflex
Trávníčková, Hana ; Bubník, Karel (referee) ; Chmelař, Milan (advisor)
The aim of this bachelor’s thesis was to clarify the phenomenon called psychogalvanic reflex, describe mechanism of its origin, its affects and use in practice. It analyzes the structure of the skin and its properties in terms of conducting electrical current, describes the phenomenon itself and discusses the conditions for its measurement. The practical part is devoted to the concept and realization of a simple psychogalvanometer. This device is connected with PC and acquired signal is processed by LabView.
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Dynamic electroneurostimulation and electrical properties of skin
Špeta, Marek ; Dvořák, Petr (referee) ; Špinka, Jiří (advisor)
The focus of this diploma thesis is on theoretical basics of dynamic electroneurostimulation, which is an effect of electrical signal on acupuncture points of skin. This technology is used in devices made by russian DiaDENS company. Practical part of this thesis covers design of human skin impedance model. Especially derivation from three-element Cole-Cole model. Elementary, there is design of equivalent circuit of constant phase element and its incorporation into circuit. Then this circuit is designed, simulated and produced. Resulting product is used as a load to measure the characteristics of the DiaDENS device.
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Realization of fingerprint scanner
Kovář, Martin ; Sekora, Jiří (referee) ; Mézl, Martin (advisor)
This master’s thesis deals with the issue of scanning human fingerprints, which is currently very topical and represents the most widespread biometric technology. The theoretical part of the work acquaints the reader with basics of dactyloscopy and biometrics and concerns technologies used for fingerprinting, image preprocessing methods and commercially available contactless optical scanners. The practical part is a realisation of a contactless optical scanner based on a Raspberry Pi minicomputer, implementation of preprocessing algorithms in Python and testing of the device from the perspective of image quality.
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Bussines Plan of Handmade Leather Bags O´gar
Dudová, Nikola ; Hrach, Vlastimil (referee) ; Šimberová, Iveta (advisor)
This thesis has been elaborated as a proposal of a business plan for establishment of a small production company in the area hand - made leather bags. The business plan is a result of analysis based on an environment business as well as the primary method. Theoretical resources of this thesis deal with the basic concepts related to business activities. Form part contains the business plan itself and the prospects of realisation of this business intention.
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Measurement of thickness of skin and subcutaneous tissue in B-mode ultrasound image
Moldříková, Zuzana ; Štohanzlová, Petra (referee) ; Šrámek, Jaromír (advisor)
In the first part this bachelor thesis deals with theoretical analysis of terms, which are needed for understanding and algorithm proposition for skin thickness and subcutaneous tissue direct measurement. Second part of this thesis deals with implementation of algorithm. In six chapters there are described terms like skin and subcutaneous tissue, adiposity, Body Composition Analysis, ultrasound, B-mode and basics of image processing, which are going to be needed for processing of ultrasound image and algorithm creation. Chapter seven includes description of algorithm implementation and description of its structure. There is also analyzed clinical verification of functionality on phantom and some volunteers. The chapter is concluded with evaluation and discussion of gained results.
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Encapsulation of active substances into nanofibers and possibilities of their application
Procházková, Lucie ; Pernicová, Iva (referee) ; Skoumalová, Petra (advisor)
The master thesis was based on the optimization of the production of nanofiber covers and to gaine the product for subsequent functional use. The production of nanofiber covers was made by electrospinning and forcespinning from selected materials. Polyhydroxybutyrate, gelatin, chitosan and alginate were used as starting materials. After successful optimization, these materials were enriched with active ingredients ampicillin and ibuprofen for the functionalized use of covers for more effective wound healing. The theoretical part was focused on the issue of skin, healing processes, types of wounds and nanofibers, the characterization of selected starting materials for the formation of nanofibers was also mentioned. The practical part was based on the lengthy optimization of the preparation of fiber covers and later enriched with active ingredients. Furthermore, combined covers made of different materials with contents of both active ingredients were designed. This was followed by the characterization of all prepared covers from the point of view of stability in the short and long term. The gradual release of active ingredients was determined spectrophotometrically and by hifh performance liquid chromatography. It was also important to determine the antimicrobial activity of selected active substances. At the end of all testing, combined coatings containing both active ingredients were used for safety testing with human keratinocyte cells (HaCaT). Safety testing was based on determining the viability of human cells using the MTT test, to verify the LDH test. A scratch test was also performed, a wound healing test after the application of devised combined covers.
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Liveness Detection on Finger Using Various Light Sources
Vančo, Petr ; Kanich, Ondřej (referee) ; Drahanský, Martin (advisor)
The purpose of this work was to increase quality, security and reliability in biometric systems. This work presents electric and optical characteristics of a human finger. In the first part of this work you can find a theoretical basis about biometric systems, a skin and light. The next part is devoted to electric characteristics of a human finger. The third part of this work describes design, implementation and results of testing optical characteristics of a finger. In the end you can find information about some of the improvements for the future.
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