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Improvement of the laboratory boards for BDTS subject
Hynek, David ; Kledrowetz, Vilém (referee) ; Pavlík, Michal (advisor)
This work describes improvements in laboratory tasks that will be used in the university classes BDTS. Theseis classes deal with diagnostics and testing of electric circuits. Main focus is on simplification of the measurement procedure. An innovation, design, firmware development and realization are described in following chapters. The theoretical part explains functions of analog circuits and failure of electronic components. The last part focuses on testing of new laboratory tasks and on modifying laboratory manuals.
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Electrochemical biosensor for the study of DNA methylation
Petrula, Jakub ; Hrdý, Radim (referee) ; Hynek, David (advisor)
This bachelor’s thesis deals with design and optimalisation of custom biosensor for detection of methylated DNA. Teoretical part explains the mechanism and importance of DNA methylation. Next section describes analytical methods used in connection with DNA methylation and some basic direct and indirect methods of detection. Final part is dedicated to experiment itself, which is divided into several sections. Section one deals witch modification of working electrode and optimalisation of detection method. Second section introduces two different ways of DNA methylation detection. First is based on direct detection and second one on detection through the biosensor. Final part shows determination of methylcytosine from sample based on analysing characteristic attributes of signal and numeric algorithm based on curve fitting.
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Influence of liposomal platinum cytostatics on cancer cells – voltammetric study
Laníková, Petra ; Prášek, Jan (referee) ; Hynek, David (advisor)
Aim of this thesis is voltammetric study influence of liposomal platinum cytostatics on cancer cells. One of the goals is summarize available informations about influence of cisplatine on cancer cells, its encapsulation into liposome and affection of this cytostatic cisplatin encapsulated in liposome on cancer cell lines. In literary recherche is detail description of these issues. Than is there specification of voltammetric methods, which serve to electrochemical detection of cisplatin. Based on literary recherche was chosen the best method for detection and subsequently the method was optimalized and than was applied to measuring itself.
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Nanopatterned alumina-based materials for electrochemical sensors and biosensors
Kynclová, Hana ; Hynek, David (referee) ; Trnková, Libuše (referee) ; Prášek, Jan (advisor)
The doctoral thesis is focused on basic research and development of nanostructured surfaces prepared using anodic alumina material. Various types of gold nanostructured surfaces and nanoporous aluminum membranes for electrochemical sensors and biosensors were prepared using the anodic oxidation method. Nanostructured surfaces were prepared by electrochemical anodization of aluminum material to form hexagonally arranged nanopores. Gold was then deposited into the nanoporous masks by electrochemical reduction from potassium dicyanoaurate solution using a pulse deposition method. The prepared nanostructured gold surfaces were electrochemically characterized by electrochemical impedance spectroscopy and voltammetry. Temperature stability and the effect of annealing on their electrochemical behavior at atmospheric pressure as well as in the vacuum were investigated. Then, gold nanostructures of various dimensions were prepared and the influence of their shape and dimensions on the electrochemical behavior was studied. Nanostructured surfaces were also modified with 11–mercaptoundecanoic acid, and the effect of this modification on the electrochemical results was studied. In the last part of the work, nanoporous aluminum membranes were prepared, and their permeability was studied.
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Portable digitally controlled stabilized source of symmetrical voltage
Hynek, David ; Drexler, Petr (referee) ; Mikulka, Jan (advisor)
This thesis deals with a design of a power supply for a measuring equipment for a geophysical mapping intended to do a reconstruction of an electrical impedance of a ground environment of water reservoirs. The power source is going to be used for laboratory measurements and for outdoor experiments. The thesis describes the selection of suitable components, equipment design and testing. The theoretical part explains the functions of used components and the principle of an electrical impedance tomography. The conclusion summarizes the properties of the proposed solution and evaluates the final product.
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Processing of electrochemical metallothionein signals from Brdicka reaction
Dvořáček, Jiří ; Hynek, David (referee) ; Valla, Martin (advisor)
This thesis deals with the Brdička electrochemical reactions and the possibilities of description and processing of metallothionein. The first part deals only marginally, an introduction to the subject, a separate occurrence of this reaction, the discovery of the properties and functions of metallothionein in the human body, describe the possibilities and applications of cluster analysis on the measured electrochemical signal of metallothionein Brdička reactions and detection of the selected peak. The second part is based on requests made program to evaluate response Brdiča working on the basis of two detection signals from within.
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Nanopatterned alumina-based materials for electrochemical sensors and biosensors
Kynclová, Hana ; Hynek, David (referee) ; Trnková, Libuše (referee) ; Prášek, Jan (advisor)
The doctoral thesis is focused on basic research and development of nanostructured surfaces prepared using anodic alumina material. Various types of gold nanostructured surfaces and nanoporous aluminum membranes for electrochemical sensors and biosensors were prepared using the anodic oxidation method. Nanostructured surfaces were prepared by electrochemical anodization of aluminum material to form hexagonally arranged nanopores. Gold was then deposited into the nanoporous masks by electrochemical reduction from potassium dicyanoaurate solution using a pulse deposition method. The prepared nanostructured gold surfaces were electrochemically characterized by electrochemical impedance spectroscopy and voltammetry. Temperature stability and the effect of annealing on their electrochemical behavior at atmospheric pressure as well as in the vacuum were investigated. Then, gold nanostructures of various dimensions were prepared and the influence of their shape and dimensions on the electrochemical behavior was studied. Nanostructured surfaces were also modified with 11–mercaptoundecanoic acid, and the effect of this modification on the electrochemical results was studied. In the last part of the work, nanoporous aluminum membranes were prepared, and their permeability was studied.
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Portable digitally controlled stabilized source of symmetrical voltage
Hynek, David ; Drexler, Petr (referee) ; Mikulka, Jan (advisor)
This thesis deals with a design of a power supply for a measuring equipment for a geophysical mapping intended to do a reconstruction of an electrical impedance of a ground environment of water reservoirs. The power source is going to be used for laboratory measurements and for outdoor experiments. The thesis describes the selection of suitable components, equipment design and testing. The theoretical part explains the functions of used components and the principle of an electrical impedance tomography. The conclusion summarizes the properties of the proposed solution and evaluates the final product.
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|
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Improvement of the laboratory boards for BDTS subject
Hynek, David ; Kledrowetz, Vilém (referee) ; Pavlík, Michal (advisor)
This work describes improvements in laboratory tasks that will be used in the university classes BDTS. Theseis classes deal with diagnostics and testing of electric circuits. Main focus is on simplification of the measurement procedure. An innovation, design, firmware development and realization are described in following chapters. The theoretical part explains functions of analog circuits and failure of electronic components. The last part focuses on testing of new laboratory tasks and on modifying laboratory manuals.
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