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název v anglickém jazyce není uveden
Benešová, Petra ; Langmeier, Miloš (advisor) ; Hach, Petr (referee) ; Kozler, Petr (referee)
Using hislochemical analysis (NADPH-diaphorase) we have been investigating the influence of intraperitoneal administration of kainic acid, hypoxia and combination of both these factors on neurons of the hippocampus and on the primary auditory cortex in male rats of the Wistar strain. Kainic acid was administered to 12-day-old and I8-day-old animals, which were exposed to long-lasting repeated hypoxia from the 2nd till the 17th day of age in a hypobaric chamber (for 8 hours a day). At the age of 22, 90 and 365 days, the animals were transcardially perfused with 4% paraformaldehyde under deep thiopental anesthesia. Cryostate sections were stained to identify NADPH-d positive neurons that were then quantified in CA1 and CA3 areas of the hippocampus, in the hilus, dorsal and ventral blade of the dentate gyrus and in the primary auditory cortex. In 22-day-old animals with kainic acid administered 18th day of life both hypoxia and kainic acid increased the number of NADPH-d positive neurons in the hilus, CA1 and CA3 areas of the hippocampus and in the primary auditory cortex. On the contrary, kainic acid given to the hypoxic animals lowered the number of NADPH-d positive neurons in the dentate gyrus. In 90-day-old animals with kainic acid administered 18th day of life hypoxia and kainic acid given to both,...
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Prague Faculties of Medicine and Their Clinics in 1938-1945
Svobodný, Petr ; Hlaváčková, Ludmila (advisor) ; Hach, Petr (referee) ; Míšková, Alena (referee)
The developments that took place in Prague in 1939-1945 offer a particularly interesting starting point for analysing the impact which changing political circumstances can have on universities. The progress of events at Prague universities and institutions of higher learning at key points of the period of 1938/39-1945 does not readily lend itself to any sort of 'straightforward' characterisation that would take us from a Nazification of the German (and Austrian) universities after 1933 to their eventual de-Nazification after 1945, or from a closing of universities of the occupied country to their eventual re-opening after 1945 (as was, for example, the case in Poland). The parallel existence of both a Czech and a German university (and technical university), their complementary and at times strongly diverging histories at key moments of the 20th century, supply a rich source of material for a study of the complex interplay between politics, university education, and science. Present intention of this dissertation, however, is not to present these universities as a whole. It focuses just on their faculties of medicine, including their clinical background in Prague hospitals. Faculties of medicine have a unique position not only with respect to other parts of a university, but also in other areas of...
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Quantification in descriptive and experimental study of functional
Tonar, Zbyněk ; Kočová, Jitka (advisor) ; Hach, Petr (referee) ; Míšek, Ivan (referee) ; Křen, Jiří (referee)
Dissertation abstract The dissertation thesis consists of seventeen papers dealing with quantitative histological assessment of vascular wall and with application of morphometry in atherosclerosis research as well as in biomechanics of elastic arteries. History of microscopic morphometry with an emphasis on stereology is reviewed in the Introduction. The principles of mathematical morphology and of stereological estimation of volume, surface, area, length, and numerical density of objects in tissue blocks are explained in the Methods. The key rules of unbiased sampling of histological series and designing morphometric studies are summarized. The distribution of the overall observed variance in biological experiments, its sources and management are discussed. Procedures necessary for three-dimensional reconstructions based on scanned histological series are described. We proved that inflammatory process was significant in patients with ruptured aneurysm of abdominal aorta (AAA). There were more pronounced inflammatory infiltrates in areas of neovascularization in ruptured AAA than in asymptomatic AAA patients. Histological findings were in agreement with higher cytosol cytokine levels (IL-6, IL-8, TNF-α), enhanced collagen III metabolism and degradation in ruptured AAA. We found lower volume fraction of the...
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Prague Faculties of Medicine and Their Clinics in 1938-1945
Svobodný, Petr ; Hlaváčková, Ludmila (advisor) ; Hach, Petr (referee) ; Míšková, Alena (referee)
The developments that took place in Prague in 1939-1945 offer a particularly interesting starting point for analysing the impact which changing political circumstances can have on universities. The progress of events at Prague universities and institutions of higher learning at key points of the period of 1938/39-1945 does not readily lend itself to any sort of 'straightforward' characterisation that would take us from a Nazification of the German (and Austrian) universities after 1933 to their eventual de-Nazification after 1945, or from a closing of universities of the occupied country to their eventual re-opening after 1945 (as was, for example, the case in Poland). The parallel existence of both a Czech and a German university (and technical university), their complementary and at times strongly diverging histories at key moments of the 20th century, supply a rich source of material for a study of the complex interplay between politics, university education, and science. Present intention of this dissertation, however, is not to present these universities as a whole. It focuses just on their faculties of medicine, including their clinical background in Prague hospitals. Faculties of medicine have a unique position not only with respect to other parts of a university, but also in other areas of...
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Quantification in descriptive and experimental study of functional
Tonar, Zbyněk ; Kočová, Jitka (advisor) ; Hach, Petr (referee) ; Míšek, Ivan (referee) ; Křen, Jiří (referee)
Dissertation abstract The dissertation thesis consists of seventeen papers dealing with quantitative histological assessment of vascular wall and with application of morphometry in atherosclerosis research as well as in biomechanics of elastic arteries. History of microscopic morphometry with an emphasis on stereology is reviewed in the Introduction. The principles of mathematical morphology and of stereological estimation of volume, surface, area, length, and numerical density of objects in tissue blocks are explained in the Methods. The key rules of unbiased sampling of histological series and designing morphometric studies are summarized. The distribution of the overall observed variance in biological experiments, its sources and management are discussed. Procedures necessary for three-dimensional reconstructions based on scanned histological series are described. We proved that inflammatory process was significant in patients with ruptured aneurysm of abdominal aorta (AAA). There were more pronounced inflammatory infiltrates in areas of neovascularization in ruptured AAA than in asymptomatic AAA patients. Histological findings were in agreement with higher cytosol cytokine levels (IL-6, IL-8, TNF-α), enhanced collagen III metabolism and degradation in ruptured AAA. We found lower volume fraction of the...
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název v anglickém jazyce není uveden
Jandová, Kateřina ; Langmeier, Miloš (advisor) ; Mareš, Jan (referee) ; Hach, Petr (referee)
Hypoxia of the brain as well as the subsequent reperfusion can seriously alter the tissue microenvironment and result of the functional and structural changes of nerve and glial cells. Results of experimental studies show that some ions (e.g. Mg2+) can interfere with the brain development. To answer the question whether magnesium can modulate changes of neuronal circuits induced by hypoxia and reperfusion effect of magnesium administration on the density of nitrergic neurons (NO synthesising neurons) in the rats exposed to repeated hypoxia during the postnatal ontogeny (12, 25, and 35-day-old) was studied. NO synthesising neurons were identified according to the presence of NADPH-diaphorase (NADPH-d), the enzyme co-localized with NO synthase. Results have shown that the long-lasting intermittent hypobaric hypoxia brings about the increase of the density of NADPH-diaphorase positive neurons in all studied regions of the hippocampus in 12-day-old animals, in 25-day-old only in the hilus of the dentate gyrus, and in 35-day-old in CA1, CA3 hippocampal regions and in the ventral blade of the dentate gyrus. Contrary to that, decreased density of nitrergic neurons was found in groups of animals exposed to hypoxia till the age of 25 days in both blades of the dentate gyrus and in 35 days in the ventral blade and...
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název v anglickém jazyce není uveden
Benešová, Petra ; Langmeier, Miloš (advisor) ; Hach, Petr (referee) ; Kozler, Petr (referee)
Using hislochemical analysis (NADPH-diaphorase) we have been investigating the influence of intraperitoneal administration of kainic acid, hypoxia and combination of both these factors on neurons of the hippocampus and on the primary auditory cortex in male rats of the Wistar strain. Kainic acid was administered to 12-day-old and I8-day-old animals, which were exposed to long-lasting repeated hypoxia from the 2nd till the 17th day of age in a hypobaric chamber (for 8 hours a day). At the age of 22, 90 and 365 days, the animals were transcardially perfused with 4% paraformaldehyde under deep thiopental anesthesia. Cryostate sections were stained to identify NADPH-d positive neurons that were then quantified in CA1 and CA3 areas of the hippocampus, in the hilus, dorsal and ventral blade of the dentate gyrus and in the primary auditory cortex. In 22-day-old animals with kainic acid administered 18th day of life both hypoxia and kainic acid increased the number of NADPH-d positive neurons in the hilus, CA1 and CA3 areas of the hippocampus and in the primary auditory cortex. On the contrary, kainic acid given to the hypoxic animals lowered the number of NADPH-d positive neurons in the dentate gyrus. In 90-day-old animals with kainic acid administered 18th day of life hypoxia and kainic acid given to both,...
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