National Repository of Grey Literature 26 records found  previous11 - 20next  jump to record: Search took 0.00 seconds. 
Protein fractionation and relative quantitation using PF 2D and ITRAQ for biomarker quest
Gadher, S. J. ; Skalníková, Helena ; Halada, Petr ; Řehulka, Pavel ; Chmelík, Josef ; Kovářová, Hana
ProteomeLab PF 2D System - Protein Fractionation in 2 Dimensions (Beckman Coulter, Fullerton, CA, USA) has been developed to fractionate complex protein mixtures by chromatofocusing in the first dimension followed by high-resolution non-porous silica reversed phase chromatography (RP LC) in the second dimension. Despite the high-resolution power of ProteomeLab PF 2D, UV-based quantitation could be compromised due to possible co-elution of several proteins into one fraction. Hence, we present an optimized protocol for application of isobaric tags for relative and absolute quantitation (iTRAQ) and MALDI-TOF/TOF mass spectrometry to obtain quantitative data from peptides derived by tryptic digestions of intact proteins fractionated by ProteomeLab PF 2D technique. To demonstrate the feasibility of such an approach, protein expression patterns obtained from the ProteomeLab PF 2D fractionation of human T-lymphoblastic leukemia CEM cell line were utilised.
Proteomic identification of posttranslation protein modifications during the malting process
Bobálová, Janette ; Chmelík, Josef
Identification and investigation of posttranslational changes of nonspecific lipid transfer protein 1, nonspecific lipid transfer protein 2 and protein Z (major endosperm albumin) from barley malt by the proteomic strategy were performed.
Determination of carbohydrates in vegetables by MALDI-TOF mass spectrometry
Štikarovská, Markéta ; Chmelík, Josef
MALDI-TOF MS was used for characterization of oligosaccharides in samples of Jerusalem artichoke and red onion. Seven selected matrices were tested. The optimization of experimental conditions was carried out for three carbohydrates that are important for food chemistry ů dextran, inulin and starch (low glucose syrup).
Influence of cultivars on distribution of starch granules
Psota, V. ; Chmelík, Josef ; Bohačenko, I.
LALLS and GFFF were used for determination size distribution of starch granules in cereal grains. Knowledge of distribution is important for breeders and malters.
Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
Čáslavský, Josef ; Chmelík, Josef
MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples.
Combination of chromatographic techniques and TOF analyser
Novák, P. ; Chmelík, Josef
This contribution deals with on-line and off-line combinations of chromatographic techniques with Time-of-Flight (TOF) analyzers.
Proteomics of barley proteins by combination of in-gel digestion and MALDI-TOF MS
Řehulka, Pavel ; Chmelík, Josef ; Mayrhofer, C. ; Allmaier, G.
It is shown that combination of gel electrophoresis, matrix assisted laser desorption/ionization time-of-flight mass spectrometry and database searching is a suitable procedure for rapid protein identification. This approach let us to identify some proteins from barley leaves and grains. Identification of barley proteins can be useful for characterization of varieties of malting barley which is important especially in food and brewing industry.
Determination of saccharides in beverages by capillary electrophoresis and MALDI-TOF mass spectrometry
Žídková, Jitka ; Přibyla, Lubomír ; Chmelík, Josef
A fast method for the detection of addition of cheap sweeteners in beverages based on combination of matrix assisted laser desorption/ionisation time of flight (MALDI-TOF) mass spectrometry with capillary electrophoresis has been developed. Capillary electrophoresis has been employed to detect the ratio of concentration of major saccharides. Application of these techniques to orange juices is presented.

National Repository of Grey Literature : 26 records found   previous11 - 20next  jump to record:
See also: similar author names
1 Chmelik, J.
8 Chmelík, Jakub
3 Chmelík, Jakub Evan
6 Chmelík, Jan
13 Chmelík, Jiří
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