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The effect of green tea polyphenols on antioxidant enzymes in mice in vivo
Kvita, Vojtěch ; Svobodová, Hana (advisor) ; Matoušková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Vojtěch Kvita Supervisor: PharmDr. Hana Bártíková, Ph.D. Title of diploma thesis: The effect of green tea polyphenols on antioxidant enzymes in mice in vivo Antioxidant enzymes belong to compounds with proven effects on human organism. Their main purpose is to defend cells against oxidative stress. In order to support this process, we are taking substances called antioxidants either in regular diet or as dietary supplements. Recently there are a lot of companies on the market, offering the supplement, promising wight loss or defence against cancer. Green tea contains many natural substances, such as polyphenolic compounds which include catechins, which have proven effects on our bodies, but the specific way they act on selected antioxidant enzymes is still matter of study. The aim of this work was to determine the effect of green tea polyphenols on antioxidant enzymes in mice. We studied 8 groups (4 normal, obese 4) wherein the two groups were left as a control which means without administration of Polyphenon. Three groups were given a Polyphenon enriched diet and for various period of time (28 days, 3 days) and at different concentrations (0.1%, 0.01%). Activity was studied in liver...
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Social Inequalities in the Jakarta Metropolitan Area
Horňáková, Marie ; Temelová, Jana (advisor) ; Svobodová, Hana (referee)
This bachelor thesis deals with social inequalities in the Jakarta Metropolitan Area. It is focused on the problematic of social exclusion in relation with the distribution of different groups of the population in the metropolitan region - especially on the differences between core and peripheral areas. Monitoring of the social differences is set in the context of post-suburbanisation and the entire work is based on theories of postmodern urbanism. In the case of segregation of population it is primarily focused on the main reasons leading to this process - differences in ethnicity and ensuring security. In the context of higher degree of security it focuses on other indicators, which could be expected to be related with it. Data from the Indonesian Bureau of Statistics have been used in the processing. The results indicate that differences exist not only between the core and suburban areas, but also within the edges themselves. Key words: Jakarta Metropolitan Area, post-suburbanisation, postmodern urbanism, social exclusion
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The impact of selected isoflavonoids on the effectiveness of anticancer therapy in MDA-MB-231 cell line
Lokvencová, Kateřina ; Svobodová, Hana (advisor) ; Matoušková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Kateřina Lokvencová Title, Name, Surname of tutor: PharmDr. Hana Bártíková, Ph.D. Title of a diploma work: The impact of selected isoflavonoids on the effectiveness of anticancer therapy in MDA-MB-231 cell line. Breast cancer is the most common cancer disease in women. There is an extensive amount of therapeutic regimens for the treatment of this disease, but it is often limited by lack of efficiency or serious side effects. Isoflavonoids genistein (GEN), daidzein (DAID) and formononetin (FORM) belong among the natural substances with bioprotective effects on the human body. Some epidemiological studies have confirmed the protective effect of isoflavonoids against certain types of cancer, including breast cancer. The aim of this work was to study the effect of isoflavonoids on cancer cells and assess their ability to support the effect of cytostatics, namely anthracycline antineoplastic agents doxorubicin (Dox). For the experiments was chosen the breast cancer cell line MDA-MB-231. To study the effect of isoflavonoids on tumor cells we chose concentrations of 1 and 10 µM which is according to the bioavailability of isoflavonoids. We have shown that isoflavonoids...
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The impact of selected isoflavonoids on the activity of enzymes participating in cytostatic drugs biotransformation
Verešová, Erika ; Svobodová, Hana (advisor) ; Lecová, Lenka (referee)
1 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Erika Verešová Supervisor: PharmDr. Hana Bártíková, Ph.D. Title of diploma thesis: The impact of selected isoflavonoids on the activity of enzymes participating in cytostatic drugs biotransformation More and more people are suffering from cancer. Scientists are striving to turn to nature and find the help there. One of the possible answers to their questions would be usage of natural substances in either prevention or even treatment of cancer. Isoflavonoids, polyphenolic compounds occurring e.g. in soybeans, are one of them. Many scientists have already tested their influence on the human body. This thesis focuses on the possible effect of isoflavonoids on the activity of enzymes participating in biotransformation of cytostatic drugs. Experiments were performed on human breast cancer cell lines MCF-7 and MDA- MB-231 exposed to isoflavonoids for 24, 48 and 72 hours. Cell line MCF-7 showed greater changes in the enzymatic activity than MDA-MB-231. Of the four enzymes tested (AKR1A1, AKR1C, CBR and GST), two enzymes showed no change in activity after exposure to isoflavonoids - enzymes CBR and AKR1C. Only one experiment brought the desired result for all isoflavones, genistein, daidzein...
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The effect of flavonoids on selected biotransformation enzymes
Procházková, Eliška ; Szotáková, Barbora (advisor) ; Svobodová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Eliška Procházková Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: The effect of flavonoids on selected biotransformation enzymes In recent years, catechins which belong to flavonoids have been studied polyphenolic constituents of green tea. Especially their beneficial effects on the human health are mostly studied. The popularity of dietary supplements with high levels of flavonoids is growing and thus they can affect a wide range of biotransformation enzymes and also the metabolism of many drugs. The aim of this diploma thesis was to find out how the selected catechins - catechin (C), epicatechin (EC), epicatechin-3-gallate (ECG), epigallocatechin (EGC), epigallocatechin-3-gallate (EGCG) and the green tea extract - polyphenon 60 (P) influence the viability of the Caco-2 cells and the activity and expression of the selected biotransformation enzymes in these cells - cytochromes P450 (CYP) isoforms 1A1, 1A2, and 3A, aldehydreductase (AKR1A1), aldo-ketoreductases 1C (AKR1C), carbonylreductase 1 (CBR1), UDP-glucuronosyltransferase (UGT), sulfotransferase (SULT) and glutathione S-transferase (GST). The viability tests (NRU test and MTT test) proved that catechins...
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Biotransformation of selected anthelmintics in sheep tapeworm (Moniezia expansa)
Němečková, Aneta ; Svobodová, Hana (advisor) ; Lamka, Jiří (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Aneta Bečanová Supervisor: Mgr. Hana Bártíková, Ph.D. Title of diploma thesis: Biotransformation of selected anthelmintics in sheep tapeworm (Moniezia expansa) Biotransformation of anthelmintics is a process that prevents the parasite from adverse effects of xenobiotics. Therefore, it is the main factor that causes the reduction of an anthelmintic effect, resistance development and thus the failure of drug therapy of helminthosis. Therefore many studies focused on anthelmintics biotransformation in helminths have been published. This experiment was aimed at the biotransformation of albendazole, flubendazole and mebendazole in the sheep tapeworm (Moniezia expansa) and the activity of its biotransformation enzymes. Tapeworms were obtained from sheep intestines. Biotransformation of benzimidazoles was determined in in vitro and ex vivo experiments. Flubendazole and mebendazole were metabolized in much greater extent than albendazole. The activity of antioxidative enzymes, which also can participate in drug biotransformation, was detected mainly in the cytosol. Neither cytochromes P 450 nor flavin-containing monooxygenases were detected. Reductive enzymes were determined by means of specific...
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Biotransformation of selected anthelmintics in sheep tapeworm (Moniezia expansa)
Bečanová, Aneta ; Svobodová, Hana (advisor) ; Lamka, Jiří (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Aneta Bečanová Supervisor: Mgr. Hana Bártíková, Ph.D. Title of diploma thesis: Biotransformation of selected anthelmintics in sheep tapeworm (Moniezia expansa) Biotransformation of anthelmintics is a process that prevents the parasite from adverse effects of xenobiotics. Therefore, it is the main factor that causes the reduction of an anthelmintic effect, resistance development and thus the failure of drug therapy of helminthosis. Therefore many studies focused on anthelmintics biotransformation in helminths have been published. This experiment was aimed at the biotransformation of albendazole, flubendazole and mebendazole in the sheep tapeworm (Moniezia expansa) and the activity of its biotransformation enzymes. Tapeworms were obtained from sheep intestines. Biotransformation of benzimidazoles was determined in in vitro and ex vivo experiments. Flubendazole and mebendazole were metabolized in much greater extent than albendazole. The activity of antioxidative enzymes, which also can participate in drug biotransformation, was detected mainly in the cytosol. Neither cytochromes P 450 nor flavin-containing monooxygenases were detected. Reductive enzymes were determined by means of specific...
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Nursing Care of Children with Orofacial Clefts
Svobodová, Hana ; Hošťálková, Monika (advisor) ; Babická, Marcela (referee)
In the theoretical part I deal with cleft lip and palate, their distribution, risk factors, prevention, diagnosis and tretment. It also the nursing care of children with cleft lip/palate the infant and toddler period. The main objective of the thesis is to determine what is the nursing care for children with orofacial cleft. In addition, three sub-objectives have been established. The first goal was to find out what the nursing care is in the field of nutrition in children with cleft face. The second goal was to survey, what the nursing care of is wound in children after orofacial surgery. The last goal was to learn about the parents' awareness of the care with facial cleft. All objectives have been met. For the qualitative research as a method for data collection, was used nursing care report. The default method for the assembly of two case reports, was conducted and interview with mothers of children according to the structure of the model by M. Gordon - Model functional health patterns. Furthermore, the method of observation was used to compile the said case reports, and so was used medical documentation. Key words: orofacial cleft, children, nursing care, prevention, congenital anomalies
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