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Multivalentní glykomika v imunoterapii experimentálních nádorů
Vannucci, Luca ; Pospíšil, Miloslav ; Krist, Pavel ; Křen, Vladimír ; Huliková, Katarína ; Luptovcová, Martina ; Svoboda, Jan ; Kuldová, Markéta ; Bezouška, Karel ; Rossmann, Pavel ; Difato, Francesco ; Mosca, F. ; Fišerová, Anna
Lectin-like receptors can modulate the cytotoxic, regulatory and dendritic cell responses by their interactions. GN8 multivalent glycoconjugates are able to internalize inside and activate NKR-P1 positive cells inducing production of IFN-gamma and expression of CD69. The final effect is a modulation of anticancer immunity with reduction of cancer growth
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Interakce glycodendrimérů s nádorovým mikroprostředím
Pospíšil, Miloslav ; Vannucci, Luca ; Luptovcová, Martina ; Svoboda, Jan ; Huliková, Katarína ; Kuldová, Miloslava ; Bezouška, Karel ; Krist, Pavel ; Pešta, M. ; Fišerová, Anna
Potentiation of immunological parameters by multivalent glycodendrimers (PAMAM-GlcNAc8) against experimental mouse melanoma and rat colon or glioma cancer. PAMAM –GlcNAc8 induce biological effects at different levels of cancer microenvironmental components and concern both, effector and cancer cells
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Protein chimistry and massspectromtry in strictural analysis of large proteins
Bezouška, Karel ; Man, Petr ; Pompach, Petr ; Novák, P. ; Havlíček, Vladimír ; Hilgenfeld, R. ; Plíhal, Ondřej ; Sklenář, Jan ; Pišvejcová, Andrea ; Křen, Vladimír
According to the textbook knowledge the use of automated Edman degradation and protein mass spectrometry for the determination of complete primary stricture is limited to small proteins with molecular size less than 10 kDa.
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Úloha sacharidových struktur v NKR-P1 zprostředkované interakci efektorových a cílových buněk
Fišerová, Anna ; Svoboda, J. ; Kuldová, Markéta ; Pucová, Alexandra ; Bezouška, Karel ; Pesta, M. ; Vannucci, Luca ; Pospíšil, Miloslav
Masking oligosaccharide sequences can assist transformed cells to escape immune surveillance. N-acetyl-glucosamine coated dendrimers have binding affinity for NKR-P1 receptor and can also modify cancer cell glycosylation profile. Effects of treatment on immune cell subpopulations were analyzed in blood, lymphoid tissues and organs, and inside tumor in B16F10 melanoma bearing mice. Treatment enhanced tumor cell immunogenicity, and induced maturation,differentiation and homing of cytotoxic cells to tumor microenvironment.
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