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Characterization of bacterial strains obtained in evolutionary engineering
Hrabalová, Vendula ; Sedláček, Petr (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with application of evolutionary engineering on PHA producing bacterial strains. Two bacterial strains, Cupriavidus necator H16 and Halomonas halophila, were chosen for the evolutionary experiments. Copper cations (Cu2+) and sodium chloride (NaCl) were chosen as the selective pressure for C. necator H16; acetic acid (AA) and levulinic acid (LA) for Halomonas halophila. The adapted strains were during long-time evolutionary experiments characterized by GC-FID and SEC-MALS. The growth of the adapted strains was studied by the mean of optical density measurement. The amount of viable cells was determined by spectral FC after their expositon to selected stress factors. Specific enzyme activities of enzymes involved in citrate and glyoxalate cycle, enzymes generating NADPH, LA metabolism enzyme and PHA biosynthesis enzymes were determined. The adapted strains were compared with the wild-type of strains. The successfull adaptation of C. necator H16 adapted to Cu2+ was detected. Biomass and PHA production of both wild and adapted H. halophila strains cultivated in lignocellulosis waste were determined. It was found out that H. halophila adapted to the LA is capable of producing more PHA than the wild strain of this bacteria.
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The Adaptation of the BIO farm object
Kurczová, Silvia ; Vystrčil, Jan (referee) ; Šuhajda, Karel (advisor)
My final thesis elaborates on the project documentation of a renovated building, which was before a farm. The building is situated in the cadastral territory of the country Velké Kunětice and will have two above-grounds after the renovation. One section of the building will be topped with an extensive green roof, and the other will be equipped with a gable roof. The building will have a cuboid shape and will serve multiple purposes, including a residential flat for the owner, office space, a repair shop, and an agricultural warehouse. The original construction system is wall structural, which will be saved and filled with new load-bearing structures. The original structure of the ceiling over the first above-ground is made of ceramics board HURDIS, which is supported by steel beams. The new ceiling over the repair shop will be monolithic reinforced concrete. The building is insulated with an ETICS contact insulation system. Vertical structures consist of original autoclaved aerated concrete and ceramics blocks and new structures of sand-lime and aerated concrete blocks. The foundations of the building are made of foundation strips.
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Employee Adaptation in the Selected Company
Romanová, Eva ; Čuta, Jaromír (referee) ; Konečný, Štěpán (advisor)
This master’s thesis deals with the adaptation process of employees in the selected company. The theoretical part is focused on explaining the issues of basic concepts related to this topic. I analyse adaptation process of Company XY a.s. in practical part and then I present proposals in this area that could help improve the company’s adaptation process.
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Unsupervised Adaptation of Speech Recognizer
Švec, Ján ; Karafiát, Martin (referee) ; Schwarz, Petr (advisor)
The goal of this thesis is to design and test techniques for unsupervised adaptation of speech recognizers on some audio data without any textual transcripts. A training set is prepared at first, and a baseline speech recognition system is trained. This sistem is used to transcribe some unseen data. We will experiment with an adaptation data selection process based on some speech transcript quality measurement. The system is re-trained on this new set than, and the accuracy is evaluated. Then we experiment with the amount of adaptation data.
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Revitalization of the castle Uherčice
Křídová, Nikola ; Šindlar, Jiljí (referee) ; Hrabec, Josef (advisor)
This diploma project deals with architectural study and functional use of the Uherčice castle grounds with regard to its character, location in the countryside and in the village, preservation of monuments and requirements of the inserted functions, as well as the creation of new opportunities for visitors and the reconnection to life of the inhabitants of the Uherčice village. And as a place of rest, education, employment and as a re-center of the area. A proposal of modification of the selected part of the castle and the park with the restoration of the French garden in its original place is a part of the project. The reconstruction of the former orangery and the new greenhouse building are proposed. Re-build greenhouse is attached with an apartment for the gardener. The second greenhouse complements one of the castle's axes and is designed for the world's largest water lily, Victoria Amazonica. In the selected part of the chateau, I mainly dealt with its use for cultural and social events that could take place here and the facilities that would be needed, including an apartment for short-term accommodation of visitors to these events. In addition, the entrance area and the operational facilities of the castle with a castellan apartment and accommodation for tourist guides are also solved.
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Speaker Recognition in the VoIP Environment
Remeš, Jan ; Pešán, Jan (referee) ; Plchot, Oldřich (advisor)
Tato práce popisuje použití systémů pro rozpoznávání mluvčího v~prostředí VoIP, úspěšnost systému a přístupy k jejímu zlepšení. Popisuje architekturu těchto systémů, metriky pro vyhodnocení jejich úspěšnosti a klíčové komponenty VoIP z hlediska rozpoznávání mluvčího. Je zde popsáno vytvoření simulace VoIP prostředí, úspěšnost systému je vyhodnocena na datech pocházejících z různých druhů VoIP prostředí a výsledky jsou demostrovány. Adaptace a kalibrace systému je provedena a jejich přínosy zhodnoceny.
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Evolutionary engineering of polyhydroxyalkanoates producing bacteria
Nováčková, Ivana ; Kovalčík, Adriána (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with the application of evolutionary engineering to PHA producing bacterial strains. The aim of the thesis is to prepare strains adapted to levulinic acid, a selected stress factor, by methods of evolutionary engineering, and then to characterize these strains. The theoretical part deals with evolutionary engineering and polyhydroxyalkanoates predominantly. The bacterial strain Cupriavidus necator H16 was used for evolutionary experiments. Levulinic acid and levulinic acid in the presence of the MMS mutagen were applied to prepare adapted strains. Selection of mutants was evaluated on the basis of growth potential and PHA content in biomass. Polymers produced by five obtained PHA-producing mutants and control were characterized using GC-FID, SEC-MALS, DSC and FT-IR. It was found that a higher content of 3HV in the copolymer led to a lower crystallinity and hence to a lower melting point, nevertheless, only the copolymer of the M0151 strain did not fit this trend. In addition to the characteristics of the polymers, the strains themselves were evaluated from the biochemical point of view by determining the activities of selected enzymes of the citrate, glyoxalate and 2-methylcitrate cycle, selected enzymes generating NADPH, levulic acid catabolism enzyme and PHA biosynthesis enzymes. On the basis of the obtained data, the possible adaptation strategies were discussed, when the E0575 strain was most differentiated from original culture. Values of specific enzyme activities were subjected to AHC and PCA statistical analysis methods.
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Merging of Different Exposured Photographs, HDR
Šmida, Vladimír ; Juránek, Roman (referee) ; Seeman, Michal (advisor)
High Dynamic Range Imaging (HDRI) is an emerging field in image postprocessing with non-trivial relations to many different areas, such as image synthesis, computer vision, video and image processing, digital photography, special effects among others. This bachelor's thesis deals with the usage of HDRI in digital photography. Conventional digital cameras are not able to record the full tonal range of the scene. Therefore, if we want to capture a high dynamic range scene, by setting the exposure time we have to choose only a small range of tones which will be well depicted. The rest of the photograph will loose important detail. The method presented and implemented in the scope of this thesis enables us to create HDR picture with realistic values of tones out of several differently exposured photographs.
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