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Aroma active compounds of selected types of herbs
Škutová, Pavla ; Hrstka, Miroslav (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with the identification of aroma active compounds in medicinal herbs. The group consists of 5 types of herbs: lemon balm (Melissa officinalis), pot marigold (Calendula officinalis), clary sage (Salvia sclarea), milk thistle (Silybum marianum), goat’s rue (Galega officinalis). These plants are known for their positive effects in folk medicine. The SPME-GC-MS method was chosen to characterize the fragrances. During the identification of volatile compounds, emphasis was put on suspected fragrance allergens listed in EC Regulation 1223/2009 in Annex III. Overall 106 compounds were identified together with 8 allergens in lemon balm, pot marigold contained 104 compounds with 7 allergens included, 82 compounds including 5 allergens in clary sage, 73 compounds were identified in milk thistle including 6 allergens and 110 compounds with 9 allergens included in goat’s rue.
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Modern herbs in the food industry
Adamczyková, Michaela ; Řezáčová, Veronika (referee) ; Vítová, Eva (advisor)
The bachelor thesis deals with the issue of volatile (aromatic) compounds of Plectranthus spp. and Echinacea purpurea. The theoretical part focuses on the general characteristics of the above mentioned herbs, the content of their bioactive and volatile compounds, and on their importance as well as on their potential use in the food industry. Using the HS-SPME-GC-MS method, the volatile compounds of the herbs and extracts prepared from them were identified and quantified in the experimental part. A total of 11 volatile compounds were found in all the samples. The most frequent one in the sample of a fresh Plectranthus was 3-carene (13,78%) as well as in the sample of dried Plectranthus (14,85%). However, in the Plectranthus extract it was -selinene (17,84%). As for Echinacea, it is germacrene D (18,64%) in the sample of a fresh one, camphor (58,46%) in the one of dried Echinacea and -copaene (20,42%) in its extract.
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Aroma active compounds of selected types of medical herbs
Pecinová, Ester ; Diviš, Pavel (referee) ; Vítová, Eva (advisor)
This thesis deals with aroma active compounds of selected types of medicinal herbs, specifically lavender (Lavandula officinalis), peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and hyssop (Hyssopus officinalis). In the theoretical part the single herbs were characterized including botanical description, chemical composition, medicinal properties, adverse effects and the practical use; aroma active compounds present in herbs are also emphasized. Based on the literature search, gas chromatography coupled with mass spectrometry as currently the most frequently used method, was chosen for the assessment of aroma compounds. Solid Phase Microextraction was used for isolation of volatile compounds from dried herbs. Within experimental part of the work selected parameters of the SPME-GC-MS method were optimized, aroma compounds in samples of herbs were identified, and twenty four suspected fragrance allergens due to the Regulation (EC) N° 1223/2009 were also identified and quantified. Overall 69 compounds were indetified together with 2 allergens in lavender, 59 compounds including 5 allergens in peppermint, sage contained 49 compounds with 4 allergens included, 41 compounds were identified in St. John’s wort including 4 allergens and finally 64 compounds with 4 allergens included in hyssop.
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Vaporiser
Vrabec, Miroslav ; Šebesta, Jiří (referee) ; Fedra, Zbyněk (advisor)
The subject of bachelor’s thesis is the vaporisers. Vaporisers are very popular thanks them ability to extrakt pure herb essences without many undesired products. The aim of this project is design selection parts of vaporisers, eventually choiced acceptable components for them function.
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Plant-based cosmetic products
Horváthová, Iveta ; Pavelková, Renata (referee) ; Skoumalová, Petra (advisor)
The aim of this bachelor thesis was studying an antimicrobial activity of selected herbal extracts and their encapsulated forms as liposomes. Furthemore, the thesis focuses on determonation of biologically active compounds, natural pigments and antioxidant activity. 6 kinds of herbs were studied - Humulus lupulus, Matricaria chamomilla, Taraxum officinale, Bellis Perennis, Cymbopogon citratus and Rubus Fruticosus. In teoretical part, the general characteristics of herbs and their use are described, it also includes the description of active compounds, microorganisms and used methods. Practical part is focused on characterisation of herbs from the perspective of antioxidant activity, determination of totaly phenolic content and flavonoids, chlorophyll and carotenoids. For analysis of antioxidant activity, concentration of phenolic compounds and flavonoids and antimicrobial activity aqueous and 20% ethanol extracts were prepared. Based on the obtained results, selected extracts were encapsulated into various types of liposomes consisting egg yolk, soy or sunflower lecithin. Generally, ethanol extracts showed higher content of active compounds. For chlorophyll and total carotenoid content determination the highest content of natural pigments was found in Taraxum officinale. Furthemore, antimicrobial activity of extracts and liposomes against 6 strains were determined - Micrococcus luteus, Serratia marcescens, Escherichia coli, Propionibacterium acnes, Staphylococcus epidermidis and Candida glabrata. The highest inhibitory effect was found in Matricaria chamomilla and Cymbopogon citratus samples. Encapsulation effectivity, size, stability and long-term stability of liposomes after a week and two months were determined. From the perspective of zeta potential all the particles were stable and reached a good encapsulation effectivity. It turned out that after two monts of storage the particles decomposed and active compounds into surroundings were released. Selected herbs generally had high content of antioxidants and active compouds. The most suitable lecithin for encapsulation was shown to be sunflower lecithin.
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Sušárny ovoce v přidružené lesní výrobě
Peterka, Lukáš
The aim of this final thesis is to emphasize the advantages of using classic drying ovens in order to find out what types of drying ovens exist, describe them, and propose an optimal solution. The work is focused on the construction of old dryers that do not need electricity or other fossil energetic sources, possible to be built on owned or leased land. Based on the gained knowledge, the alternative proposal was designed and can be adapted and can be adapted to the time, financial and also professional requirements of its implementation. The work contains a small analysis of the purchased products associated with forest industry. The economic aspect is also taken into account in order to get an awareness of costs and possible profits. The goal is therefore to provide the inspiration for an alternative or additional use for the owners of small or a larger forest lands. The main contribution of this work is the alternative design of the dryer, its budget and plan, which is possible to implement for a better farming management.
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Využití fytoterapie v chovu koní
HAVLÍKOVÁ, Natálie
The aim of this bachelor thesis was to conduct research on phytotherapy and herbs used in horse breeding and internal parasites and their developmental stages. Breed-ers are increasingly paying attention to the health care of horses, and for that reason they tend to use unconventional medicine, such as herbs. The research focused on monitoring the effectiveness of a herbal mixture by WILD HERBS s.r.o. which supports against internal parasites, and recommenda-tions for the use of herbs in horse breeding. Monitoring took place on a farm in Mažice, where horses received a mixture fed in several stages by a feeding machine. After feeding the mixture, a coprological examination followed. Large and small strongyles, horse roundworm and horse tapeworm were found in the monitored farm. A clear indication of the effectiveness of the mixture when fed was a signifi-cant reduction in EPG values compared to previous years, which was also the goal. The EPG value in 2022 was 273 which is a significant decrease compared to the previous year of 2021 when the average EPG value was 634. The difference in EPG values between seasons was not significant. The value is rather determined by the current weather or the arrival of a new horse in the herd and the resistance of indi-viduals to the infection. The mixture had a very favorable effect on most horses, but at some point, a few individuals had higher EPG values, which could have been de-termined by several other factors. The steps needed to support a partially parasite-free breeding include collecting faeces, dragging pastures and monitoring the level of parasitic infection, giving suitable herbal mixtures and using anthelmintics in case of heavy infection.
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The influence of share of selected legumes and herbs on senzoric quality of conserved biomass
ŘÍHA, Patrik
The aim of this thesis was to evaluate the quality of preserved biomass in dependence on the composition of samples by sensory evaluation of individual quality indicators of grass silage. The silage of selected types of clovers, herbs was evaluated and compared with a sample of silage from maize. The main indicators included color, fragrance, moldy and rotting, consistency and the last point was counted the amount of dry matter in silage. These indicators were monitored on 33 samples of silage coming from the Vysočina Region and the Třebíč District. After the end of the preservation the indicators were scored and the points were added up. After adding the points, the individual samples were evaluated as successful or unsuccessful. Furthermore, the work focuses on the distribution of fodder in terms of carbohydrates and nitrogen substances, factors affecting forage quality, preservation of forage by silage, its processing technology and last but not least, the use of preservatives and their importance. Botanical images of selected meadows were also taken and the coverage of the evaluated species of clover and herbs as well as agrobotanical groups was determined.
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Suburban park Bažantnice (Kladno) in Botany and Dendrology Instruction
Veverka, Vojtěch ; Novotný, Petr (advisor) ; Hrouda, Lubomír (referee)
The topic of this thesis is forest park's Bažantnice mapping. The thesis suggests the way of aplication of botanic terrain education. Botany and dendrology is one of the main educations question on elementary and secondary school. Park Bažantnice can be used in science lesson for its hight potential. The thesis i s divided into two parts. The first of them summarizes differents types of the terrain education and focuses on didactic using of the park Bažantnice. The second one deals with the station mapping and describes way of data colecting and adapting. Accompanying pictures and generated maps are included in appendiced at the back of the thesis. Keywords: dendrology, biology, botany, education, park, Bažantnice, Kladno, mapping, herbs, woody plants, terrain education
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Modern herbs in the food industry
Adamczyková, Michaela ; Řezáčová, Veronika (referee) ; Vítová, Eva (advisor)
The bachelor thesis deals with the issue of volatile (aromatic) compounds of Plectranthus spp. and Echinacea purpurea. The theoretical part focuses on the general characteristics of the above mentioned herbs, the content of their bioactive and volatile compounds, and on their importance as well as on their potential use in the food industry. Using the HS-SPME-GC-MS method, the volatile compounds of the herbs and extracts prepared from them were identified and quantified in the experimental part. A total of 11 volatile compounds were found in all the samples. The most frequent one in the sample of a fresh Plectranthus was 3-carene (13,78%) as well as in the sample of dried Plectranthus (14,85%). However, in the Plectranthus extract it was -selinene (17,84%). As for Echinacea, it is germacrene D (18,64%) in the sample of a fresh one, camphor (58,46%) in the one of dried Echinacea and -copaene (20,42%) in its extract.
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