|
|
A comparison of metabolic activity and use of microalgae and pigment-formed yeasts
Szotkowski, Martin ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
Carotenoids are natural pigments occurring in plants and many microorganisms, such as algae, yeast and bacteria. They represent the most common group of antioxidants with significant biological effect. Lipids are an essential component of all living organisms. They are the source and the reservoir of energy for organisms, which can also be used in the petrochemical industry for the production of biofuels. This thesis deals with the production properties of carotenoids and lipids by selected strains of yeasts, algae, and canobacteria, in order to apply the acquired knowledge and find cheap suitable alternatives for microbial biotechnological production of these metabolites using waste substrates. Carotenoids, coenzyme Q, and ergosterol in cells were determined by liquid chromatography. The lipid content and fatty acid profile was analyzed by gas chromatography. Cell morphology and localization of selected metabolites were analyzed by fluorescence lifetime imaging microscopy. In this work yeast genera Sporobolomyces, Cystofilobasidium and Rhodotorula were used. As the representatives of the algae and cyanobacteria strains of Desmodesmus, Cyanothece, Chlamydomonas, Synechoccocus and Chlorella strains were studied. In the overall comparison, the yeast strains were more productive than algae and cyanobacteria. The highest carotenoid production was found in S. pararoseus, C. capitatum and R. mucilaginosa cells, while the highest lipid yield was observed in strains of C. infirmominiatum and S. metaroseus.
|
|
|
Spectrophotometric determination of phosphate in ternary systems with dyes
Novotný, Radek ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The theoretical part of this master’s thesis is focused on the characteristics and forms of phosphorus, occurrence in natural waters and the problems associated with higher phosphorus concentrations, especially in the development of cyanobacteria and algae. There are also described the possibilities of determining the degree of pollution of natural waters by nutrients and the effects of eutrophication. There are also described methods for the determination of phosphates, especially spectrophotometric determination in ternary systems with dyes. Like other methods are mentioned AAS, ion chromatography, electrophoresis and izotachophoresis, electrochemical methods or enzymatic methods. In the experimental part of this work deals with the selection and optimalization of suitable ternary system with organic dyes for spectrophotometric determination of orthophosphates. The system with phosphovanadomolybdic acid is studied too. For analytical procedure with malachite green oxalate, which was found to be most useful, were studied interfering ions, commonly occurring in natural waters . The chosen procedure was finally tested on real water samples.
|
|
|
Determination of phosphates in waters and characterization of their negtive effects on water ecosystem
Novotný, Radek ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
This bachelor thesis is focused on the negative effects of phosphates in natural waters and their methods of determination. The first part is characterized by a negative impact on natural water and there are characterized the problems associated with eutrophication, development of cyanobacteria and algae and the resulting potential health risks. The second section describes the methods by which phosphates can be determined. The work highlights the particular spectrophotometric methods such as molybdophosphoric acid, vanadomolybdophosphoric acid, wolframophosphoric acid, phosphomolybdenum blue and methods with some basic dyes such as rhodamine B, rhodamine 6G, malachite green and crystal violet. There are also mentioned methods, which are used less frequently, but they are relatively sensitive, such as AAS, AES, LC-ICP-MS and next such as chromatographic, electrochemical and enzymatic methods. Finally, work mentions the possibility of using automatic techniques such as FIA or CFA, which are suitable for routine and frequent determination, often using the spectrophotometric methods, but not only these.
|
|
| |
|
|
Study of moisture conditions in the facade wall after application of the ETICS
Ševčíků, Robin ; Steuer,, Radek (referee) ; Šťastník, Stanislav (advisor)
In the last decades the application of ETICS (external thermal insulation composite system) became a popular measure to improve the energy performance and the weather resistance of facades in the building stock. However, hand in hand with that the problem of microorganisms such as algae and fungi on facades has substantially increased in re-cent years. One of the main reasons is the interaction of climate influences with modern compositions of thermal insulation systems resulting besides of large thermal and mois-ture loads in occurrences of green covers of algae on facade surfaces. The algae popula-tion on facades with ETICS is supported by the sufficient humidity, caused by the night temperature decrease of surface to the dew-point temperature and by consequent con-densation of vapour. The thermal radiation against clear night sky is responsible for the origin of this periodically repeated phenomenon. This bachelor’s thesis is concentrated to the description of available knowledge in this field and explains the phenomenons by means of principles of building physics. Moreover, at the end, in the practical part, there are presented the results of author’s own measurements concerning determination of basic properties of commonly used fa-cade materials (spectral emissivity, absorbing power), important with regard to the sub-ject of the thesis.
|
|
|
Use of combination of bacteria and algae for wastewater treatment
Hlavsová, Barbora ; Stříteský, Lubomír (referee) ; Hlavínek, Petr (advisor)
The aim of this bachelor's thesis is to introduce the reader to the ever improving methods of biological wastewater treatment with the use of bacteria, algae and combination of both. Further it elaborates on the promising ways of utilizing biomass gained from these treatments in various sectors, such as energetics, chemistry, pharmacy and agriculture. The second part of the thesis offers a techno-economic study of wastewater treatment using combination of bacteria and algae in a specifically assigned area.
|
|
|
Adaptation of algae to extreme environments
KVÍDEROVÁ, Jana
The acidophilic red alga Cyanidium sp. is one of the dominant mat-forming species in the highly acidic waters of Río Tinto, Spain. The culture of Cyanidium sp., isolated from a microbial mat sample collected at Río Tinto, was exposed to 9 different pH conditions in a gradient from 0.5 to 5 for 24 h and its physiological status evaluated by variable chlorophyll a fluorescence kinetics measurements. The optimum pH ranged from 1.5 to 2.5. A steep decrease of the photochemical activity was observed at pH below 1 even after 30 min of exposure. At pH above 2.5, the decline was more moderate and its negative effect on photochemistry was less severe. The red alga Cyanidium sp. is well-adapted to prevailing pH at its original locality at Río Tinto, i.e. pH of 1 to 3. The short-term survival in pH<1.5 may be adaptation to rare exposures to such low pH in the field. The tolerance of pH above 3 could be caused by adaptation to the microenvironment of the inner parts of microbial mats in which Cyanidium sp. usually dominates and where higher pH could occur due to photosynthetic oxygen production.
|
|
|
Eutrophic stress of the Orlik reservoir
ZELENKOVÁ, Kamila
Detection of potential sources of load eutrophic surface waters. Orlik dam is out of your water and energy purposes also used for recreational purposes. In recent years, recorded deterioration of water quality. It happens that it appears "Flower" water surface and increase algae. The content of this work was to monitor the changing state of water quality in reservoirs Orlik and its surroundings during the season. Parameters were monitored: conductivity, pH, oxygen, nitrates, and phosphates and during the season of 2013.
|
|
| |
|
|
Pilot study on the issue of Euglenophyta in the Czech Republic
JURÁŇ, Josef
On the basis of literature review and floristic survey of almost 150 localities a revised checklist and a proposal of redlist of the Euglenophytes of the Czech republic were made. 286 taxa are reported from our state from which 5 taxa are new for Czech republic - Lepocinclis caudata, Monomorphina reeuwykiana, Phacus arnoldii, Trachelomonas bituricensis var. lotharingia, Trachelomonas volvocina var. punctata. The proposed redlist contains 9 taxa which are threatened by small number of localities or settle biotopes threatened by human impact.
|
guest ::
