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Evaluation of storage protein profiles modifications in barley (\kur{Hordeum vulgare} L.) grains during malting procedure using gel electrophoresis
BINDER, Richard
This bachelor thesis is focused on evaluation of storage protein profiles modifications in barley (Hordeum vulgare L.) grains during malting procedure using gel electrophoresis. It has been analyzed three varieties of spring barley. One nonmalting variety Herris and two malting varieties Radegast and Sebastien. There are significant proteolysis changes during the malting procedure. Theese changes of storage proteins were captured and evaluated by gel electrophoresis (SDS-PAGE) on the basis of changes in the molecular weight in the different phases of malting procedure. Next observation which has been done was the measurement of the thousand grain weight (TGW) before and after the malting procedure. Theese results have been evaluated by statistic program.
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Critical evaluation of potential of harvested product proteins in selected crops from perspective of their use in food and biotechnological applications
BUCKOVÁ, Pavlína
This diploma thesis deals with plant proteins. Literary review discusses the properties of the major proteins in selected agricultural crops (wheat, barley, oats, corn, peas, soybeans, canola, potato, Jerusalem artichoke) and their use in food, diet, biotechnology and other industries. The diploma thesis was also assessing the potential production of proteins in these crops on the basis of literature and available statistical data. Data is organized into tables and graphs
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Evaluation of Chemical and Technological Properties of Grain and Milling Franctions of Hulless Barley for Bakery Use
Vaculová, Kateřina ; Jirsa, Ondřej ; Balounová, Marta ; Sedláčková, Irena
Wider use of barley with great food potential due to its high nutritional value of grain is constrained by lack of knowledge about the functionality of barley grain components in terms of processing and food products development. Selected genotypes of hulless spring barley (cv. AF Lucius, lines KM 2084, KM 2283, KM 1057 and genetic resource Nudimelanocrithon) were examined for chemical composition (crude protein, starch, β-glucans, macro-minerals) and breadmaking properties of grain and grain milling fractions (flour, semolina, bran). Differences in chemical composition of genotypes reflected in both the yield and rheological properties of individual milling fractions, measured with farinograph and extensograph. The addition of barley flour (15-30%) or barley semolina and bran (10 and 20%) to standard wheat flour reflected in different rheological properties depending on a barley genotype. The farinograph parameters largely varied in individual materials, however, the percentage of barley fractions in the mixture did not affect the values considerably. In comparison with standard wheat flour, values of extensograph parameters were low (especially for energy and extensibility) in all barley samples studied. However, they were affected by both the proportion of barley fraction and its interaction with a rest period. When the rest period was longer (90 min), even higher additions of semolina or bran (especially in cv. AF Lucius) were technologically acceptable.
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Biodiversity of epigeic beetles on selected field crops - effects of management on the structure of communities
SVOBODA, Richard
Epigeických beetle communities were studied on four agroecosystems with different crops grown. All four agroecosystems were located in the vicinity of Czech Budejovice (South Bohemia). The first agroecosystem is conventionally grown corn. On the second one agroecosystem it was conventionally grown wheat. The third agroecosystem was barley and the fourth was permanent grassland. Individual agroekosystems differed not only in crops but also in agrotechnical operations during the capture. In all agroecosystems was for capture of the material used method of pitfall traps. Subsequently, those types have been studied. Then was studied degree of human impact on the biodiversity of species of different ecological groups found in these locations.Beetle activity was highest in the maize agroecosystem. There was found almost half of all captured individuals. In all agroecosystems was activity affected by management of agrotechnic operations and also by for that year specific weather. All sites were completely dominated by expansive species (E - types of deforested habitats strongly influenced by human activities)
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Alcohol Yield of Oat, Barley and Wheat Hydrolysates
PIKL, Miroslav
Major part of ethanol production for industrial use in Czech Republic is made from cereals. Effect of acid, alkali and enzymatic hydrolysis after thermopressure preparation takes part in the paper. The most promising alternative is enzymatic hydrolysis. Especially efficiency of different hydrolytic enzymes towards different species of cereals takes the main part of the simulations. Local farm products, oats, barley and wheat were prepaired by steam explosion in different pressure. After hydrolysis were detected the GE and after fermentation was observed the production of alcohol.
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