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The interaction of Prp22 and Prp45 proteins in budding yeast spliceosome
Senohrábková, Lenka ; Půta, František (advisor) ; Malcová, Ivana (referee)
Protein Prp22 is a DEAH box RNA helicase, which plays two distinct roles in pre-mRNA splicing: it participates in second transesterification step (ATP independent function) and it releases mature mRNA from the spliceosome (ATP dependent function). Prp45p, yeast ortholog of the human transcription co-regulator SNW/SKIP, is an essential splicing factor, it is included in spliceosome throughout the splicing reaction. Mutant prp45(1-169) genetically interacts with some alleles of NTC complex and second step splicing factors, one of them is also gene PRP22. Here we present, that mutants prp22(-158T) and prp22(-327A), which are synthetically lethal with prp45(1-169), express lower amount of Prp22p due to the mutation in upstream regulation region. Mutants prp22(-158T), prp22(300PPI) and prp22(-327A) affect splicing of pre-mRNA with mutation in 5'ss with respect to sequence of the second exon. N-terminal mutants prp22(∆301) and prp22(∆350) are synthetically lethal with prp45(1-169). Synthetic lethality is possibly caused by lower efficiency of Prp22 recruitment to the spliceosomes, which is no more viable for cells.
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Conversion of the financial statements according to the Czech accounting principles to the US GAAP and their influence on investors
Cernenco, Marina ; Žárová, Marcela (advisor) ; Procházka, David (referee)
This thesis examines the influence of financial reporting on present and potencial investors and creditors, in the context of the comparison of requirements and accounting policies applied by national regulations and U. S. GAAP. The comparison is done in the framework of the conversion of financial statements which is essensial for Czech companies simultaneously following different accounting principles. The theoretical part is devoted to examining the influence and role of financial statements prepared in accordance with accounting regulations and U. S. accounting standards, with a more detailed analysis of selected accounting areas of. Attention is also paid to the conversion process, possibilities of its implementation as well as limitations and problems associated with it. Based on the teoretical part the analysis of the conversion process is done on the example of a particular company.
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The influence of the presidential administration on the immigration regulation in France
Forstová, Jana ; Druláková, Radka (advisor) ; Novotná, Markéta (referee)
The issue of immigration and the obstacles connected with it become more and more discussed within the government as well as during the presidential campaigns. France is a country with a profound immigration history and sets a model role in the way how the immigration policy can be influenced by left-wing and right-wing governments. The influence of the presidential administration on the immigration regulation in France is the topic of the proposed thesis. The principal objective is to assess the effectivness of the regulation of the immigration policy. It aims to answer the question whether the presidential administration has influence on the immigration regulation.
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Women on the Labour Market in the Czech Republic
Zemanová, Lucie ; Kaczor, Pavel (advisor) ; Sedláková, Jaroslava (referee)
This Bachelor's Thesis subject are issues relating to women's participation in the labour market. Theory section of the Thesis addresses labour market and its functioning as a whole, development of the woman's role in society, discrimination, and, last but not least, legislative acts and other norms regulating this field.The second section of the Thesis summarizes results of a survey and finds views on the issue by a small sample of respondents. Based on these results, the Thesis makes conclusions..
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The bank as a business company
Straka, Ondřej ; Liška, Petr (advisor) ; Elek, Štefan (referee)
Bank as a Commercial Company The main aim of the diploma work "Bank as a Commercial Company"is to show a bank from a view of the company law. Its objective is not to describe all bank institutes, but to show some differences between legal regulation of joint-stock companies and banks in Czech legal regulation and to describe these differences. This aim is fulfilled by comparing the Act No. 21/1992 Coll. as subsequently amended (hereinafter referred to as the "Bank Act") with regulation of joint-stock companies established by Act. No. 513/1991 Coll., Commercial Code as subsequently amended and with some other Acts related to companies. Banks are generally traders with money. Bank Act characterizes bank as a legal entity residing in the Czech Republic, established as joint-stock company that accepts deposits and provide loans and which have a licence for exercising abovementioned business. The special role and other special characteristics of bank business create the necessity of a special legal regulation. Main aims of such legal regulation are to protect deposits of depositors, to ensure stability of banks and whole the financial sector. To fulfil above mentioned aims the legal regulation constitutes many rules, duties and institutes. These rules, duties and institutes change and accrue to the common...
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Multinational companies and their operations in developing countries
Randlová, Lucie ; Jiránková, Martina (advisor) ; Pavlík, Petr (referee)
This thesis deals with multinational companies and their operations in developing countries. The first part describes characteristics and general functioning of multinational companies, their origin, development and role in the global economy. The second section introduces economic and social structure of developing countries and analyzes the positive and negative impacts of multinational companies on economic, social and political development, culture and environment of host developing counties. The subject of the third chapter is description of two particular examples of these companies in developing countries -- Royal Dutch/Shell in Nigeria and Nike in Southeast Asia. The fourth chapter presents the possibilities of regulation of multinational corporations.
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Expression and function of molecules of innate system in embryonal phagocytes
Vavrochová, Tereza ; Filipp, Dominik (advisor) ; Černý, Jan (referee)
Phagocytes of an early embryo represent a mixture of myeloid lineages that differ from adult macrophages phenotypically, biochemically and by their origin. Recent studies suggested that there are at least three waves of macrophages populating the early embryo: a maternally-derived one and two waves of extraembryonic, YS-derived origin. In addition, the occurence of early embryonic phagocytes of undetermined origin in developing anterior head mesoderm in evolutionary distinct species is well documented. This origin-related heterogeneity among early embryonic phagocyte subpopulations coupled with the lack of specific markers makes it difficult to distinguish them phenotypically and study their potentially distinct physiological roles in early development. The aim of this study is to identify and characterize a set of novel markers suitable for identification of embryonic phagocytes. Here, using qRT-PCR approach, we have established the kinetics of expression of Toll like receptors (TLRs) and their TIR-domain containing adaptors during early embryogenesis (E7.5-E12.5) and demonstrate that their major cellular source are indeed phagocytes. Using whole-mount embryo immunohistochemistry we also show that negative regulator of TLR signaling Sigirr is expressed during very early stages of mouse...
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Analysis of novel members of FAHFA lipid class derived from omega 3-PUFA by LC-MS/MS and LC-MS/MS/MS
Rombaldová, Martina ; Kuda, Ondřej ; Březinová, Marie ; Slavíková, Barbora ; Pošta, Martin ; Beier, Petr ; Janovská, Petra ; Veleba, J. ; Kopecký Jr., J. ; Kudová, Eva ; Pelikánová, T. ; Kopecký, Jan
White adipose tissue (WAT) is a complex organ with both metabolic and endocrine functions. Dysregulation of all of these functions of WAT, together with low-grade inflammation of the tissue in obesity, contributes to the development of insulin resistance and type 2 diabetes. Omega-3 polyunsaturated fatty acids (PUFA) of marine origin play an important role in resolution of inflammation and exert beneficial metabolic effects. Using LC-MS/MS method, we elucidated the structures of novel members of fatty acid esters of hydroxy fatty acids (FAHFA), which were present in murine and human serum and WAT after omega-3 PUFA supplementation in diet. These compounds contained DHA esterified to 9- and 13-hydroxyoctadecadienoic acid (HLA) termed 9-DHAHLA and 13-DHAHLA, and were synthesized in WAT. Our results document the existence of novel lipid mediators, which are involved in the beneficial anti-inflammatory effects attributed toomega-3 PUFA, in both miceandhumans.
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Less common metabolites of steroid hormones in human physiology and pathophysiology.
Máčová, Ludmila ; Hampl, Richard (advisor) ; Rauch, Pavel (referee) ; Černý, Ivan (referee)
The thesis focuses on selected, yet unsolved question of the role of less common steroids and SHBG as a junction of three endocrine systems. Answering these questions may help to understand the complex mechanism of action of these hormones on the human organism. This thesis is based on five author and co-written studies mostly published in foreign scientific journals. In the case of studies of metabolites 16α-OH-DHEA and 7-oxo-DHEA (Zamrazilová et al., 2007; Kazihnitková et al., 2007) were focused on the development of appropriate methodological approach because recently used methods showed a low sensitivity and specificity. We developed and statistically evaluated new RIA methods which are rapid, sensitive and inexpensive. Both of them can be used in other research even in routine practice. New methods were also used to determine the metabolites in a statistically significant sample of healthy human population. In another study (Zamrazilová et al., 2010) we examined the relationships of selected steroid metabolites and SHBG in patients with CAH. We assumed that SHBG may act as a non-steroidal laboratory parameter reflecting the effectiveness of substitution therapy in these patients. Our assumption was not confirmed. We observed lower levels of SHBG which at least in women reflect effects of...
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Problem of the undernourishment and the food water resources
Polzová, Tereza ; Kuna, Zbyněk (advisor) ; Petr, Petr (referee)
The main focus of the final thesis are the problems related to the problem of undernourishment in affected
regions of the world, sea fishing and aquaculture.
In the beginning, the definition of the food problem in the world is introduced. The potential contribution of proteins from the sea production to human dietary is emphasized. Further, the exponential population growth is mentioned, influencing the future development and the food problem itself.
The second part of thesis is dedicated to fisheries and aquaculture. The importance and benefits of sea water resources as well as aquaculture is mentioned from the nutritional and livelihood point of view. Sea management and international regulations are playing
crucial role in the sustainability of the fisheries stocks. These are needed to eliminate the problems of fisheries and aquaculture and the degradation of sea ecosystems due to unsuitable fishing techniques.
At the end of the thesis a summary of all important information is listed. Followed
by possible solutions of these global problems.
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