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The integration of handicaped children and youths in the camp "Společenství harmonie těla a ducha"
Tremlová, Gabriela ; Hubertová, Lucie (advisor) ; Dočkal, Jan (referee)
The bachelor thesis deals with integration of handicaped children and youths in the camp "Společenství harmonie těla a ducha". Goal of the thesis is to present non-profit organisation "Společenství harmonie těla a ducha", to focus on issue of integration and to analyse scale of integration within the frame of particular activities of the camp. Another goal is to make a suggestion for measures for next running of the camp for the year 2014. Following basic terms are described in this thesis: "integration" and "free time in the camp". Text is divided into two part: theoretical one and practical one. Those two parts are connected through each chapters. Proposals for better integration in the year 2014 are mentioned. Closing part of the thesis summarizes the results which were found out by participating in the camp. Powered by TCPDF (www.tcpdf.org)
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Role of small effector molecules in bacterial signalling.
Kolář, Petr ; Lichá, Irena (advisor) ; Hilská, Markéta (referee)
Small effector molecules play an important role in bacterial physiology. There are many types of them in the bacterial cell. One group are small signalling molecules which participate in quorum sensing, enabling bacterial cell-cell communication as part of a extracellular signalling. These molecules mediate information about the cell density and different qualities of the extracellular matrix. Next group of small effector molecules are modified nucleotides. They participate in intracellular signalling pathways which regulate the switch of bacterial lifestyle according to changing environment. Best studied are signalling pathways using the molecules - c-di-GMP and (p)ppGpp. Detail studies were done in case of gram positive (Bacillus subtilis) and gram negative (Escherichia coli, Vibrio cholerae) bacteria, where was proved the connection between c-di-GMP signalling pathways and quorum sensing (Vibrio cholerae, Xanthomonas campestris). Important discovery in field of small effector molecules is the c-di-AMP signalling pathway in Bacillus subtilis. New regulatory mechanisms were determined in well-known small effector molecule cAMP, regarding the signalling pathways connections (Vibrio cholerae). Recent studies considering the cooperation of the extracellular and intracellular signalization pathways...
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Art, avant-garde and kitsch in works of Clement Greenberg and Matei Călinescu
Taltynová, Marie ; Kulka, Tomáš (advisor) ; Dadejík, Ondřej (referee)
Bachelor thesis Art, avant-garde and kitsch in works of Clement Greenberg and Matei Călinescu deals with kitsch in relation to avant-garde and to contemporary art, and to the visual arts in general. It is primarily based on the essay Avant-Garde and Kitsch by American critic Clement Greenberg and the essay Kitsch by Romanian literary scholar Matei Călinescu. The first part is devoted to outlining the concept of kitsch according to these two theorists, with an emphasis on the thinking about the origin and nature of kitsch. The second part deals with revising Greenberg's beliefs that condemn popular culture, official culture and academism for kitsch and highlights avant-garde as the only worthy manifestation of art of his time. On this basis, the work examines why avant-garde seemed like the pure opposite of kitsch in the thirties, and why to the contrary today we find that contemporary art is often influenced by kitsch.
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Roman Limes in Noricum and Pannonia
Salášková, Veronika ; Musil, Jiří (advisor) ; Titz, Pavel (referee)
Author's name: Veronika Salášková Instititution: Charles University, Prague Filosofical faculty Institute for Classical Archaeology Celetná 20, Prague 1 Discipline: Classical archaeology Title: Roman Limes in Noricum and Pannonia Supervisor: Doc. PhDr. Jiří Musil, PhD. Number of pages: Number of attachments: Year of vindication: 2012 Keywords: Limes Romanus, legio, auxilia, provinces, camp, castellum, castra This thesis proposes to describe origin and evolution of Roman limes in provinces Noricum and Pannonia. At first will summarize the sources, history and evolution of Roman limes generally including particular types of fortifications, their evolution and troops of Roman army from it's beginning until it's end. Then will describe sections of limes in Noricum and Pannonia. Part of the thesis is also catalogue of images.
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Assessing biochemical properties of PDE8A1: Design of experimental system in living cells"
Galica, Tomáš ; Černý, Jan (advisor) ; Mašek, Tomáš (referee)
4 Abstract Phosphodiesterases (PDEs), enzymes that hydrolyze cyclic nucleotides, are important components of signal transduction pathways in eukaryotic cells. Second messenger 3'-5'- cyclic adenosine monophosphate (cAMP) is hydrolyzed by specific PDEs. By controlling concentration levels of cAMP in cell, PDEs preserve favorable environment for successful transmission of the cAMP signal. Moreover, PDEs are activated by protein kinase A (PKA) in response to elevated cAMP concentration, which is a feature crucial for signal termination. PDE8A1 is a high-affinity cAMP-specific IBMX insensitive phosphodiesterase, an enzyme important for cAMP signaling. However, mostly due to a lack of specific inhibitor, its role has not been assessed in detail. This thesis reports cloning of PDE8A1, identification of its posttranslational modifications and subcellular localization, as well as an alternative approach to address PDE biology by the use of cyclase toxin from Bordetella pertussis. Keywords: phosphodiesterase, cAMP, posttranslational modification, myristoylation, palmitoylation, adenylate cyclase toxin
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Phenomenon camp
Černá, Lucie ; Paulíček, Miroslav (advisor) ; Holeček, Tomáš (referee)
In this work we pay attention especially to the broader introducing of the phenomenon, which is called Camp. We describe here the basic features of the camp, such as excessive efforts to effect, a possession for foiled art and his seriousness, which fails. We discuss the camp view of the surrounding world, with its typical irony, and we refer also to the current form of this phenomenon. We try to show the historical development of the camp, with particular emphasis on modernity, precisely because of his perception that it is based. Space is also devoted to the development, which can be detected in the works of various authors who deal with the theme of Camp. We focus also on specific redefinition of "good" and "bad" art, and not neglecting the relationship between camp and kitsch, as it often leads to erroneous substitution of these two phenomena. Much of the work is dedicated to taste, because camp has become sociologically relevant issue in communion with it. Here we focus mainly on the different view of the taste of "upper class" and the upper class itself. We show the connection of Camp with the theory of American authors Kern and Peterson which refers to the cultural "omnivorousness". At the same time we try to indicate where in the sociological point of view upon taste there is a place for...
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Pharmacological modifications of potential signal systems regulating metabolism of adipocytes and hepatocytes and their influence on obesity
Hodis, Jiří ; Farghali, Hassan (advisor) ; Kršiak, Miloslav (referee) ; Otová, Berta (referee)
v anglickém jazyce: Thesis abstract: Background and aims: Both obesity and metabolic syndrome form severe health problems in the whole world. Nevertheless the armament of pharmacotherapy for both diseases remains unsatisfactory. We aimed our work to main organs in risk of the mentioned diseases -liver and visceral fat using hepatocytes and visceral adipocytes as model. We detected 3 main metabolic and signalization activities- glycogenolysis, Nitric oxide (NO) production and transcription of inducible NO synthase (iNOS) in hepatocytes, lipolysis, NO production and iNOS transcription rate in adipocytes. We directed our interest to combination of peroxisome proliferation activator receptor γ (PPARγ) agonist, antagonist and β3 adrenergic agonist in the culture of epididymal rat adipocytes in the first part of our work. While in the second part we investigated the influence of β and α adrenergic mimetics, adrenergic blockers in the culture of rat high glycogen content hepatocytes. Methods: NO production was detected under the active agents treatments by detection of NO oxidative products NO2 and NO3 in media. Glycogenolysis was measured as free glucose rise released by hepatocytes into the media. NOS transcription level was extrapolated after comparative polymerase chain reaction with reverse...
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Immunomodulation of dendritic cells by adenylate cyclase toxin from B. pertussis
Jáňová, Hana ; Brdička, Tomáš (referee) ; Adkins, Irena (advisor)
Adenylate cyclase toxin (CyaA) produced by the causative agent of whooping cough Bordetella pertussis, is a key virulence factor important for colonization of the host. CyaA targets preferentially myeloid phagocytes expressing CD11b/CD18 integrin. By elevating cytosolic cAMP in the host cells, CyaA interferes with their phagocytic, chemotactic and oxidative burst capacities. Furthermore, CyaA modulates the secretion of cytokines and the maturation state in LPS-stimulated dendritic cells (DC) by affecting the expression of costimulatory molecules. In this study, we investigated the effects of CyaA on the capacity of murine bone-marrow DC to prime CD4+ and CD8+ T cells in response to ovalbumin epitopes delivered by the CyaA-AC- toxoid, as a model antigen. Further, we examined the possible impact of CyaA on the antigen uptake and processing for MHC class I and II-restricted presentation by DC, as we previously observed a decreased T cell stimulatory capacity of CyaA-treated DC in response to soluble ovalbumin. We found out that the high levels of cAMP generated by CyaA in LPS-stimulated DC account for the decreased presentation of ovalbumin epitopes carried by CyaA-AC- toxoid on MHC class I and II molecules, thereby impairing the CD8+ and CD4+ T cell responses. Whereas CyaA did not influence the...
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Role of cAMP signaling in phagocyte migration
Dáňová, Klára ; Paňková, Daniela (referee) ; Kamanová, Jana (advisor)
Cell migration plays a key role in a wide diversity of biological processes. Migration enables phagocytic cells to localize into the site of inflammation and to lymph nodes, thereby leading to initiation of innate and adaptive immune responses, respectively. The signal transduction that coordinates phagocyte migration consist of diverse signaling proteins, being often under control of 3'-5'-cyclic adenosine monophosphate (cAMP) and its two effectors, protein kinase A (PKA) and Epac (exchange protein activated by cAMP). Small GTPase Rap is activated by Epac and controls phagocyte migration via activation of RAPL and RIAM proteins. On the other hand, PKA regulates cell migration via modulation of activity of other proteins, which comprise actin, integrins, small GTPases Rho, Rac, Cdc42 as well as protein VASP. A prominent feature of cAMP signalization is its spatio-temporal organization. Therefore, besides description of cAMP-regulated signaling cascades in cell migration, this bachelor thesis also depicts how changes of activity of cAMP effectors in time and place are involved in regulation of cell movement.
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