|
Solid-phase Microextraction of Oxazepam from Plasma
Raška, Martin ; Sochor, Jaroslav (advisor) ; Štěrbová, Petra (referee)
Solid-phase microextraction of oxazepam from plasma Rigorous thesis Mgr. Martin Raška Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové This rigorous thesis deals with the issue of extracting active compounds from the biological material. It's specifically focused on the extraction of oxazepam from plasma sample (containing 10 percent of plasma) using solid-phase microextraction (SPME) followed by the analysis of extracted compound using high performance liquid chromatography (HPLC). SPME is ideal method for biological material processing. It's simple and effective sample preparation technique integrating sorption, desorption and concentration of an analyte, that doesn't require use of any solvents or complicated apparatus. The basic principle of this method is exposure of liquid or fluid sample to a small amount of extraction phase. Polydimethylsiloxan/divinylbenzen (PDMS/DVB) fiber was used for the extractions from plasma samples undertaken in the practical part of this thesis. Except the quantitative analysis there were plasma samples containing oxazepam in concentration of 0,1 mg/ml used. The optimal conditions of the extraction were determined in the first part of the practical section....
|
|
Measurement and evaluation force paraxial ray muscles in the area lumbar spine and muscles sinuses coeliac.\\
ŠEBESTA, Karel
The aim of the work was to measure and evaluate the strength of deep stabilizing system (DSS) in connection with correct body posture and, subsequently, to asses the efficiency of the existing known exercise of the lumbar spine area as well as drawing comparison to the exercise recently introduced by doc. PaedDr. Pavel Kolář. The research was carried out on two sample groups of sixth form boys attending elementary school. It was necessary in the first place to detect, test and document correct body posture and, above all, the functionality of the DSS in members of the respected groups. This was followed by six weeks of active exercise according to exercise programme focusing primarily on the lumber spine area. The control group of ten boys carried out exercises based mainly on general principles of correct exercise whereas the experimental group totalling the same number of boys did exercises of DSS and lumbar spine area according to doc. PaedDr. Pavel Kolář. A muscle dynamometer was used to objectify and check the DSM in lumbar spine area. The measuring was than documented, processed and evaluated by means of charts and graphs. The work also comprises an overview of basic principles of lumbar spine stability as well as a questionnaire designed to assess health and lifestyle.\\
|
|
Implementation of Antiplatelet Therapy Monitoring on the Analyzer Multiplate
Trenčanská, Šárka ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
This study describes the implementation of antiplatelet therapy monitoring on the analyzer Multiplate that works on the principle of impedance aggregometry. We used to determine four tests - ASPItest used for monitoring acetyl salicylic acid (ASA) therapy, ADPtest and ADPtestHS used to monitoring thienopyridines treatment and TRAPtest , which is used to monitoring treatment with GPIIb/IIIa inhibitors. The aim of this study was to verify the accuracy of this method for individual tests, to determine its own reference limits and compare them with literature data and on a group of cardiac patients receiving dual antiplatelet therapy (aspirin 100 mg per day and clopidogrel 75 mg per day) to assess any resistance to these drugs. Accuracy of the method we verified the group of 10 blood donors without any treatment. Each sample was repeatedly measured 5 times and from the measured values we calculated coefficients of variation. Our values of coefficients of variation from the values given by the manufacturer (CV 6 - 10 %) did not differ significantly (ASPItest: CV 8.7 %, ADPtest: CV 8.8 %, ADPtestHS: CV 13.3 % and TRAPtest: CV 12.1 %). We also verified on a group of 32 blood donors without any treatment reference ranges AUC for single tests, which were compared with reference limits from the literature....
|
|
Clause order in contemporary Czech
Chudobová, Martina ; Lehečková, Eva (advisor) ; Zikánová, Šárka (referee)
My thesis deals with the word order in the contemporary Czech language. The aim is to discover the principles that control the clause order within sentences and to find out to what extent the position of the main and subordinate clauses is fixed. The first section summarizes the work of Czech and Slovak linguists and introduces the principles that apply to the clause order in particular sentences. It defines types of sentences with fixed, usual and loose order. At the end of this part there is an overview of the positions of different types of clauses, which became the springboard for the empirical part. The second section deals with the clause order of selected subordinate sentences. I have chosen a representative sample of connecting expressions. Using materials of the Czech National Corpus I try to outline the contemporary Czech clause order of the most frequent types of clauses following these expressions.
|
|
Sexual size dimorphism in medieval period in Bohemia.
Kaupová, Sylva ; Brůžek, Jaroslav (advisor) ; Dobisíková, Miluše (referee)
Differences in height among human populations or changes in height over time may be associated with different degrees of sexual dimorphism in this feature. This thesis compared the height and sexual size dimorphism (SSD) between two groups of the population of Medieval Bohemia - rural residents and inhabitants of Prague. A sample of 179 adult skeletons from five Prague sites and 175 individuals from four rural sites, dating from the 11th to 14th century, was used. Sex was determined following the principle of primary and secondary sex diagnosis, which enabled the derivation of population-specific discriminant functions using the dimensions of the humerus, femur, tibia and talus. A subsequent analysis of body height did not show statistically significant differences either in height or in SSD between the Prague and the rural part of the population of Medieval Bohemia, despite the major changes which Czech Medieval society went through in the 13th century. Comparing our results with previously published data on the height of the Czech population during the early modern period indicates a statistically significant reduction in the magnitude of sexual size dimorphism in the population of the17th century, particularly associated with a decrease in the height of men. A significantly higher stature of...
|
|
On the mechanisms of shrinkage reducing admixtures in alkali activated materials
Komosná, Kateřina ; Bartoníčková, Eva (referee) ; Kalina, Lukáš (advisor)
This thesis is focused on the principles of behavior of shrinkage reducing agents (SRA) in alkali-activated materials based on blast furnace slag. The main focus of this work is selecting the most suitable admixture based on experiments, by which will be achieved through minimal shrinkage and will have negative effect on the properties of alkali-activated blast furnace slag at the same time. In experimental section of this work, the surface tension as individual additives as their mixtures with pore solution were measured primarily. Then the testing samples composed of blast furnace slag, water glass and addition of SRA were prepared. Of these samples was measured shrinkage and weight loss. Moreover, their mechanical properties such as flexural and compressive strength were monitored. Next, the beginning and the end of solidification was studied using the Vicat device and last but not least workability. The hydration process of alkali-activated materials with SRA was measured calorimetrically. Finally the microstructure in prepared samples was observed using scanning electron microscopy (SEM-EDS) and the total porosity was determined by mercury porosimeter.
|
|
Communicative strategies of politicians in interaction.
Lokajová, Jana ; Šaldová, Pavlína (advisor) ; Klégr, Aleš (referee)
Abstract. The aim of this M.A. thesis is to apply on the sample of five transcribed interviews with American politicians the typology of strategies devised by social psychologists P. Bull and K. Mayer (1993) for 'non-replies' of British politicians in the genre of a political interview and to determine whether this typology could be qualitatively correlated to specific linguistic means (the use of passive, pronominal shifts, hedges). The responses of politicians are examined within the CDA method also in relation to the macro-principles of evasion (dissimulation), coercion, legitimation and delegitimation, which are claimed to be valid in political discourse by P. Chilton (2004) in order to discover whether Bull and Mayer's social typology could be related to these principles and to the strategies of face-management (Brown and Levinson 1987). It is expected that politicians will boost their positive image in the interview and coerce the public in the agenda shift (Clayman, Heritage 2002) through every response in the interview. A question which according to Bull and Elliott (1996) consists of face threat is also expected to be attacked; quantitative results are presented which verify this assumption. In addition, as thirty strategies were observed to have been employed by Mrs Thatcher and Neil Kinnock in...
|
|
Quality of Nursing Care - administration of medicaments
Chocholoušová, Božena ; Schneiderová, Michaela (advisor) ; Dvořáčková, Ivana (referee) ; Wasyliwová, Vlasta (referee)
This bachelor paper is focused on the quality of the provided nursing care during application of medicaments. It provides data on the quality of nursing care, nursing standards, important information on the medicaments field, ways and principles of their administration. The focus of the thesis is constituted by a quantitative research of the nurses' knowledge of the medicaments field and the quality of the provided nursing care when administrating medicaments; the result is the presentation of a proposal of a standard nursing procedure when administrating medicaments per os. The analysed sample is constituted by medical staff of the general nurse branch during the execution of their profession in a Central Bohemia health care institution.
|
|
HPLC Analysis of Benzimidazoles Using Core-shell Columns I
Oslejová, Kateřina ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of biophysics and physical chemistry Candidate: Kateřina Oslejová Supervisor: ing. Vladimír Kubíček, CSc. Title of diploma thesis: HPLC Analysis of Benzimidazoles Using Core-shell Columns I The aim of this work was to transfer the HPLC method of determination of flubendazole and its metabolite, i.e. reduced flubendazole, from a classical C18 column (250×3,0 mm; 5 µm) to core-shell column Ascentis® Express C18 (10×3,0; 2,7 µm) and to find a suitable internal standard. Experiments were performed using liquid chromatograph Agilent 1200 Series® with both DAD and fluorescence detection. Flubendazole was detected with the DAD detector. Low concentrations of reduced flubendazole were detected with the fluorimetric detector. Several mobile phase compositions were tested. The mobile phase consisting of phosphate buffer (pH = 3,0; 0,025 mol/l) and acetonitrile (70/30, v/v) appeared to be a good choice for the separation under study. Two internal standards were exploited for the quantification, depending on the detection principle. Mebendazole served as the internal standard in the case of DAD detection, while oxibendazole was utilized for fluorimetric detection. The proposed method was successfully applied for analyses of real samples.
|
|
Size-exclusion chromatography - analytická metoda pro charakterizaci rozpuštěných organických látek
PILSOVÁ, Klára
The main aim of this bachelor thesis is the application of size-exclusion chromatography for a measurement of the molar mass of organic matter in water samples from Bohemian Forest lakes and subsequent determination of iron, aluminium and phophorus con-centration by inductively-coupled plasma/mass spectrometry. In the theoretical part, there is information about organic matter in water and the principles of size-exclusion chromatography and ICP/MS. The experimental part deals with the procedure of organic matter measurement and the determination of iron, aluminium and phosphorus concentration. Afterwards, the procedure of SEC and ICP/MS data processing is described and the results are discussed and compared with other analytical techniques.
|
guest ::
