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Strategie oplozování u ryb s vnějším oplozením
SIDDIQUE, Mohammad Abdul Momin
Morphological properties, species specific differences, development, and function of egg envelopes are of importance for a better understanding of fertilization strategies as well as for captive reproduction. We reviewed morphology and the developmental stages of egg envelopes, mechanism of polyspermy block, and also the role of micropyle and cortical granules in polyspermy block for acipenserid eggs. The structure of the egg envelope is similar among the acipenserids, comprising an external envelope (thecal cells, basal lamina, and follicular epithelium), a five-layered oocyte envelope (adhesive layer, alveolar layer, epilayer, and zona radiata externa and interna) and a layer of oocyte matrix and cortical granules. The development of acipenserid egg envelope within the ovary comprises five stages, with further changes following fertilization. Moreover, we standardized the terminology used to describe the egg membranes which can minimize the confusions and be helpful for future work on acipenserids eggs as well as for other fish species. The sperm to egg ratio (required to fertilize eggs) and effects of pre-incubation of eggs in freshwater before fertilization were studied to standardized fertilization protocols for sterlet Acipenser ruthenus. Pre-incubation time had no effect on fertilization success at 430,000:1 and 43,000:1 sperm to egg ratios, while it was significant at the 4300:1 and 430:1 ratios. The use of adequate experimental suboptimal sperm to egg ratio revealed a positive effect of pre-incubation time, such that at the 430:1 ratio, 0.5 min pre-incubation increased the fertilization rate than 10 min. Transmission electron microscopy showed that pre-incubation of eggs in water for <10 min did not trigger a cortical reaction, suggesting that a low sperm to egg ratio 0.5 to 1 min pre-incubation of eggs in freshwater prior to fertilization can enhance fertilization rate of sterlet (minimally do not change fertilization). The effects of pre-incubation in seawater and the duration of egg receptivity were determined for fresh and over-ripe sea bass eggs. Our results revealed a significant effect of pre-incubation time for both the fresh (P < 0.01) and over-ripe eggs (P < 0.01). The fresh eggs had a higher fertilization success than over-ripe eggs. Fertilization success of eggs significantly declined for both of these treatments after 3 min of pre-incubation clearly indicated that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. Effects of pre-incubation of eggs and activation medium on the percentage of eyed embryos for Ide Leuciscus idus were examined. At the eyed-egg stage, pre-incubation time was significant for the freshwater activation medium (P < 0.001), such that the percentage of eyed embryos declined across the pre-incubation time gradient. Activating medium had a significant effect on the percentage of eyed embryos for each pre-incubation time (P < 0.05). More precisely, freshwater produced the lowest percentage of eyed embryos at all pre-incubation times, whereas saline water and Woynarovich solution produced the highest percentage of eyed embryos at 0 s and 30 s before incubation. Examination of sperm traits showed no impact of activating medium on computer assisted sperm analysis parameters. Our results suggested that saline water or Woynarovich solution improve fertilization rate in Ide during fertilization. In conclusion, this thesis provides basic knowledge on gamete biology, role of egg receptivity period, effects of pre-incubation of eggs and different activating medium which can be useful to understand the fertilization strategies of different externally fertilizing fishes as well as standardize their fertilization protocol for captive reproduction.
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Supplier management in automotive industry
Holubcová, Romana ; Štůsek, Jaromír (advisor)
In my thesis I deal with the process of Supplier management in automotive industry. Supplier - customer relationship is very important for a success both company and for the global development of the economy too. These relationships must be partnership and support the development of the companies and their each other businesses are beneficial for both sides. In my thesis I analyze current process of the supplier management in the company, what directly supply to OEM. Original Equipment manufacturing (OEM), is the final producer of automobiles, it is mean this company do not produce components but assemble supplied modules. I propose improvements and up-date the directive Strategic purchasing according to results from the analysis.
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Evaluation of FGMRI provenance plot with European beech (Fagus sylvatica L.) on the locality Jíloviště, Baně (Central Bohemia)
Kuklová, Alena ; Podrázský, Vilém (advisor)
Buk lesní (Fagus sylvatica L.) je nejvýznamnější listnatou dřevinou České republiky. V minulých letech se jeho zastoupení velmi snížilo v důsledku nešetrné těžby, přírodních disturbancí a následně hlavně změnou lesního hospodaření, kdy se upřednostňovaly především jehličnaté dřeviny smrk ztepilý (Picea abies) a borovice lesní (Pinus sylvestris). Provenienční výzkum se, díky jejich preferování při pěstování, nejprve věnoval hlavně jehličnatým dřevinám, až v pozdější době se zaměřuje i na dřeviny listnaté. V současné době se naše lesní hospodářství k buku znovu vrací a jeho zastoupení se v našich lesích pomalu opět zvyšuje.
Úkolem diplomové práce je provedení terénního šetření na provenienční výzkumné ploše VÚLHM Strnady č. 82 Jíloviště, Baně ve věku 34 let (zjištění počtu rostoucích jedinců, tloušťkového a výškového růstu, tvárnosti kmene, větvení, úhlu větví, tloušťky větví), zjištěná a naměřená data statisticky zpracovat a vyhodnotit rozdíly mezi testovanými proveniencemi buku lesního.
V první části práce je zpracována literární rešerše, zaměřena na provenienční výzkum buku lesního, v další části jsou statisticky zpracovány kvantitativní a kvalitativní znaky zjišťované na výzkumné ploše. V poslední části jsou vyhodnocená data porovnávána s daty z minulých let.
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Electronic auction in healthcare
Vyoral, Marek ; Toth, Štefan (advisor) ; Jan, Jan (referee)
Working with the theoretical part focuses on the regulation of electronic auctions in terms of legislation both Czech and foreign, given that we are members of the European Union. Furthermore, the work explains the possible ways to use electronic auctions, which are mostly used in both the Czech Republic and abroad. At work I also am trying to shed light on the current use of auctions on the auction portal Aukro examples, which is widely used by people for personal use. Finally, the theoretical part I try to outline the future development of electronic auctions. The practical part is focused primarily on its activities in the hospital Pardubice Region, Inc. where I introduced the auction. In several examples, visible results, which not only brings centralization entire purchase, but also the use of electronic auctions as a tool for communication and bidding suppliers with the lowest purchase price for a group of hospitals. The work also draws from the Ministry for Regional Development, which is responsible for the preparation of the most important regulations for electronic auctions in healthcare, namely the Public Procurement Act. Electronic auctions are a tool very interesting, because it is the most transparent way of evaluating procurement by parameters rates. Whether if electronic auction in health care and efficient purchase option will be discussed in this work.
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Effect of feeding flaxseed on the quality of goat milk fat
Malá, Kateřina ; Fantová, Milena (advisor)
Type of feed, its quality, form of adjustments and feeding technique influence the composition and quality of milk. The share of milk components is highly variable, depending on nutrition and animal health. The greatest changes occurring in milk fat content. Milk fat is responsible for the natural flavor of milk. However, in terms of the modern world, human, saturated fatty acids contained in milk fat is considered negative active ingredient with the subsequent development of cardiovascular disease and the development of atherosclerosis in humans. Currently, therefore a growing demand for polyunsaturated fatty acids in milk as a healthy alternative. For this reason, an effort to influence the composition or the content of fatty acids in the milk of dairy animals. One way to significantly increase the content of desired MUFA and PUFA acids in milk fat is the use of oils and oilseeds in the ration. Very positive influence flaxseed, which among other things contains high levels of linolenic acid.
The aim of this work is to analyze and evaluate the milk fat of goats, which are fed with linseed in extruded form compared with a control group of goats without complementary food. It can assume that feeding flaxseed to increase milk fat content, including omega-3 and omega-6 fatty acids.
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Revitalization of the chateau park in Choltice
Čermáková, Michaela ; Vaněk, Jan (advisor) ; František, František (referee)
This work has the task to assess the current situation of the chateau park and at the same time the game preserve in Choltice, and suggest its revitalization in order to rejuvenate the vegetation and making the field available for the general public. Choltice is a market town in the Pardubice region, approximately 15 km west from Pardubice. At the end of 17. century a baroque castle was built here, which belonged to the count Simon Thun. His family belonged it until 1945. The chateau and the park is now owned by the borough Choltice. The chateau neighbours with the extensive English park, which passes the chateau game preserve, which is since 1992 a nature reserve and an important European locality.
The diploma thesis contains a detailed theoretical basis and proposes solutions, which are directed both on the protection of the living conditions of protected and endangered species of a beetle Osmoderma eremita. This species occurs only rarely and his survival area is very limited, therefore it is necessary to create those conditions, and it is currently one of the main tasks of the revitalization.
The next step is to help revitalize the introduction of spontaneous breeding fallow deer in the game preserve. This idea is not new, the fallow deer were bred there until 1945. The town of Choltice is very inclined towards this idea about farming and supports it. To increase the number of visitors this diploma thesis contains a proposal for the creation of a nature trail, this area would attract wider public than so far.
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Appaloosa breeding and their utilization
Zuzjaková, Eva ; Neumann, Cyril (advisor) ; Starostová, Lucie (referee)
This bachelor thesis is a summary of the basic information about breeding and utilization of appaloosa. A dominant characteristic of appaloosa is an attractive color coat pattern, which is caused through a set of genes called Leopard Complex (Lp). That is the reason, why in this thesis, at first, the history of presence of the Lp in genus Equus takes place. The presence of Lp can be found already in many cave paintings of prehistoric horses. During domestication, the coat pattern became important. The horses of the same color coat pattern, which have today appaloosa, are depicted in the Celts, Scythians and Etruscans tombs, as well as in murals of Chinese and Egyptian cultures. Significant breeding of the spotted horses are also documented in the Ferghana Valley in eastern Uzbekistan. In Europe at 16th century horses were expanded and a lot of them have spotted coat pattern. Then the Spanish settlers imported these horses to the America, where Nez Perce Indian tribe bred horses, which we know today as Appaloosa.
Furthermore, in this thesis the Appaloosa breed organization, which belongs complete to Appaloosa Horse club of America, is described. The breeding standard and characteristics, which are color coat pattern, mottled skin, visible white sclera and striped hooves are inducted. Seven various types of coat pattern and tree types of registration are also described.
The major problems in the appaloosa breed are inherited diseases. In this thesis the most serious five of them are identified. It is CSNB, congenital stationary night blindnes associated with a homozygous variant of LP gene. HYPP, hyperkalaemic periodic paralysis, which causes muscle spasm of affected horses. This disease was obtained from quarter horses. HERDA - Hereditary Equine Regional Dermal Asthenia which causes irreversible damage to exposed skin. Another serious disease is PSSM, polysaccharide storage myopathy, which is one of many myopathy occuring in horses. The latter disease is lethal GBED. Affected foals lack the enzyme GBE which is necessary for the synthesis and storage of glycogen. In spite of the improving precautions, these diseases have not been fully eliminated from breeding.
Further, there are given the possibilities of using Appaloosa horses in sports, hobby and ranch works. The system of shows and their ratings is described as well.
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The Probation and Mediation Service in the Practice of the Czech Republic
Veselá, Kateřina ; Hájková, Ivana (advisor) ; Zdeněk, Zdeněk (referee)
Probation and mediation service is an institution which has been gaining increasingly vital and important position in the field of criminal law. The service focuses on a very wide range of subjects and its activities aim not only to monitor but also to help. It realizes the execution of alternative sentences and also shows activity in pre-trial proceedings and in proceedings before the court decision. It cooperates with many different organizations, it is involved in many projects. For criminal justice it has become an essential and equal partner in dealing with the consequences of crimes committed by both adult offenders and juveniles. It contributes to the favourable development of the re-education of accused and convicted people, supervises proper execution of sentenced alternative punishments and the achievement of their purpose. It makes sure that the collective process was fulfilled to deal with all the adverse consequences of illegal acts of the offenders and that the victims utilize their legal rights and entitlements. Probation and mediation service provides a comprehensive view of both criminal activities and the personalities of offenders.
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Supplier management in automotive industry
Holubcová, Romana ; Štůsek, Jaromír (advisor)
In my thesis I deal with the process of Supplier management in automotive industry. Supplier - customer relationship is very important for a success both company and for the global development of the economy too. These relationships must be partnership and support the development of the companies and their each other businesses are beneficial for both sides. In my thesis I analyze current process of the supplier management in the company, what directly supply to OEM. Original Equipment manufacturing (OEM), is the final producer of automobiles, it is mean this company do not produce components but assemble supplied modules. I propose improvements and up-date the directive Strategic purchasing according to results from the analysis.
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Resistance of cole vegetable cultivars against Plasmodiophora brassicae pathotypes in Czech Republic
Zacharová, Lea ; Kazda, Jan (advisor) ; Gerhard, Gerhard (referee)
Clubroot disease of crucifer plants is caused by a pathogen Plasmodiophora brassicae (Wor.). It is counted among the most common and most serious diseases that attack not only rape, but also, for example, head cabbage, cauliflower, broccoli and other kinds of cabbage vegetables.
Plasmodiophora brassicae is descirebed as a very durable and well-developed body in this thesis. Physical, chemical and biological components of the soil environment are in the thesis associated with an effect on survival, growth and reproduction of this microbe. Obviously, Plasmodiophora's brassicae successfulness is given due to its resting spores and because of them the microbe is able to survive in a soil for years.
The experimental part of my thesis is focused on a testing of resistance of selected cultivars against Plasmodiophora brassicae. Each variety were grown under the control within conditions in the greenhouse. To evaluate the degree of a damage of the plants scale of Buzacki (1975), Strelkov et al. (2007) were applied, the index of an invasion was calculated based on these scales.
Cultivars were exposed to a pathotype 6 and as a control variety was used the cultivar Granaat. The results had shown the most susceptible respond of a cultivar red cabbage F1 Loder and other varieties tested had confirmed their resistance to Plasmodiophora brassicae.
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