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Changes in status Gypsy woman in a family
PROKEŠOVÁ, Věra
The Work deals with status of gypsy woman in a family during 20. century till present. It is consisted of two main parts. In the first part are mentioned basic informations about configurate of gypsy community, status of gypsy woman by traditional ceremonies and customs, parts, status and diference of status betwen individual members in traditional gypsy family. There are mentioned important period of 20. century, whitch are expressive influence on changes and development of position gypsy woman in family. There is mentioned status of gypsy woman in the present at the end of the first part. The informations from interview with gypsies womans are started the second part. The result is, that status of gypsies womans in the family is still change and develop. In the present gypsies womans mostly have not inferior status in the family, but thay have equivalent status with theirs husbands or boyfriends.
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Autobiography in the novels by Luis Sepúlveda
Šoubová, Petra ; Housková, Anna (advisor) ; Vydrová, Hedvika (referee)
This diploma thesis deals with the presence of autobiographical techniques in selected novels by the Chilean writer Luis Sepúlveda (Patagonia Express, etc.). The first part focuses on the theoretical approach to autobiography. Acquired information will be subsequently applied to Sepúlveda's novels. The analysis of selected novels relates Sepúlveda's novels both with his biographical background and with literary tradition, i.e. it examines particular techniques which traditionally belong to autobiograpfy: ways to evoke autobiographical impression in narration, prounoun forms, dealing with space and time.
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PCA3 and its use in the diagnosis of prostate cancer.
Škultétyová, Jana ; Semecký, Vladimír (advisor) ; Herink, Josef (referee)
Škultétyová Jana PCA3 and its importance in diagnostics of prostate cancer Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Field of study: Medical laboratory technician Prostate cancer is a global problem that bothers many men. Diagnosis is currently focused on measuring levels of prostate-specific antigen, which is used as a screening test. It is focused on the diagnosis of rectal examination and positive biopsy findings. Pitfall of this diagnosis is its not being specific, because prostatic antigen also secretes non-malignant cells. The risk is not finding the proper biopsy tissue, and it often leads to repeat the biopsy. Modern science is therefore looking for other diagnostic options. The determination of PCA3 gene which expresses specifically in prostate cancer cells looks like prospective option. While existing studies point to the estimated positive predictive value of biopsy, which would allow us to indicate their unnecessary and thus save patients from further invasive procedures. This work contains information about diagnosis of prostate cancer, briefly describes PCA3 analysis and summarizes the results obtained by the laboratory in which I work. Keywords: prostate cancer, PCA3, PSA, biopsy
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Preparedness of the Czech Republic Police as a Fundamental Element of the Integrated Rescue System for Solving Emergency Situations
MELICHAR, Zdeněk
The study analyzes the status quo of the Czech Republic Police (PCR) as a fundamental element of the country{\crq}s Integrated Rescue System, especially on the level of its executive units of territorial competence that most often encounter and have to solve such situations. Furthermore, the study points out the areas where the PCR should improve its readiness to handle extraordinary events and suggests ways of implementing suitable solutions. One part of the study describes the status quo of the PCR primarily in the area of education, legislation, access to information, material provisions, and collaboration with other entities on solving emergency situations. The status quo of the PCR members{\crq} preparedness and scope of their knowledge about the relevant issues was explored using the method of a questionnaire survey, supplemented with interviews amongst the policemen at the Regional Directorate of the Police of the South-Bohemian Region. The results were thereupon compared with the set-out objectives, legislative provisions in the given area, and the results of other surveys. The data obtained confirmed my hypothesis or presumption that there is a lot of room for improvement in the level of preparedness of the PCR as a fundamental element of the Integrated Rescue System, especially in the area of policemen{\crq}s awareness of all the issues of concern. On the basis of the information obtained, certain measures were proposed with the aim of improving the situation, mainly by means of relevant training modules for policemen working in the field, as well as those on the different levels of management, publishing relevant comprehensive materials and easily accessible information, and creating methodological aides, primarily for policemen serving directly in the field.
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Public spaces in suburbia: case study of Prague's hinterland
Havlíčková, Barbora ; Špačková, Petra (advisor) ; Fialová, Dana (referee)
This thesis focuses on the appearance of public spaces in the suburban communities in northeastern hinterland of Prague. The main objective is to describe and evaluate the importance of public spaces and use a specific group - women with children. Research into public spaces is based on several assumptions made in the introduction, which are compared with findings from empirical research collected qualitative method. The results showed that public spaces in the suburban communities exist and are useful. Important role is attributed to civil society organizations that use public spaces for social events that lead to their recovery and promote the development of social interaction. Keywords: public spaces, women with children, useful, social interaction
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Chicken antibodies as a tool of cytochrome P450 immunodetection
Mácová, Iva ; Hodek, Petr (advisor) ; Koblas, Tomáš (referee)
Cytochrome P450 is an important enzyme which catalyzes a large amount of reactions of endogenous and exogenous metabolism. There are compounds which increase expression of this enzyme and it leads up to the carcinogen activation and cancer formation. It includes much propagated chemopreventive compounds which are consumed abundantly in dietary supplements. It is therefore important to have the methods for determination of cytochrome P450 induction. One of these methods is the cytochrome P450 immunodetection by Western blotting. The detection of proteins on the membrane is done by the specific antibodies mostly gained from mammals. In this bachelor thesis there is the evidence that the antibodies from hen egg serve as well as the mammalian antibodies. The cytochromes P450 1A1 and 1A2 was detected by these egg antibodies after rat exposure to the model chemopreventive compounds β-naphthoflavone.
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Study of effect of Bacillus subtilis yxkO gene on motility during stress response to osmotic upshift.
Streitová, Eliška ; Lichá, Irena (advisor) ; Krásný, Libor (referee)
Bacillus subtilis is gram-positive soil bacteria. In its natural environment it is constantly exposed to changes of chemical and physical conditons, including changes of osmolality. It responds to high osmolality by transporting of potassium ions and afterthat transporting and/or synthetising of compatible solutes. In last years the mutant strain Bacillus subtilis L-42 was isolated with non-specific insertional mutagenesis (mini Tn10) in our laboratory. This strain displays limited growth and inability to cope with hyperosmotic shock in a defined medium with potassium concentration of < 1 mmol/l. Insertion of transposon was located in yxkO gene which encodes a protein of unknown biological function. Some other data also indicate a possible role of disruption of yxkO gene in regulation of expression of hag gene, which encodes flagelin - a pivotal protein of bacterial flagellum. The goal of this thesis was to clarify if the disruption of yxkO gene influences motility and whether is affected the transcription of hag gene. With integrative vector pMUTIN4 a mutant strain with specific mutation of yxkO gene was prepared. Vector was pasted into chromosome of Bacillus subtilis strain 1A839 - genotype of this strain allows to extrude the known transcriptional regulation of hag gene. Cell's motility was...
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The role of macrophages in immunosuppression mediated ny regulatory T cells
Kadlecová, Kristýna ; Holáň, Vladimír (advisor) ; Stříž, Ilja (referee)
Regulatory T cells (Treg) represent one of the most important mechanisms of immunoregulation. Treg suppress immune reactions and prevent overactivation of the immune system. There is a lot of ways of Treg action described, here we have focused on Treg interference with macrophages. The suppressor capacity of a highly purified Treg population was demonstrated in proliferation assays. The level of suppression of effector T cell proliferation differs depending on the presence of macrophages in the culture. Treg suppression has been significantly higher in the presence of macrophages. These observations led to hypotesis that Treg affect directly macrophages. However, using flow cytometry, reduction of expression of costimulatory molecules on macrophages after culture with Treg was not observed. Macrophages precultured with Treg showed a comparable functionality as macrophages cultured alone. Neither flow cytometry nor live cell imaging revealed any cytotoxic activity of Treg towards macrophages. Despite the presence of macrophages, Treg did not suppress effector cell proliferation in a model, where stronger activation of effector cells was induced. Therefore, a new hypothesis was presented - initially observed higher suppression in the presence of macrophages was probably caused by a qualitatively or...
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Representation of time in cartographic works on the example of maps of historical battles
Vít, Lukáš ; Bláha, Jan Daniel (advisor) ; Bayer, Tomáš (referee)
Representation of time in cartographic works on the example of maps of historical battles Abstract This study contains an in-depth analysis of possible graphical presentation of the fourth cartographic dimension (time) and utilizes the acquired findings in prototypical animated maps of historical battles. The initial chapter reflects cartography in general, later, the area of animated maps is in focus. The first part of the text is dedicated to the connection of cartography and time. Practical means of time representation are described. Further, a recommended general pattern is specified for design and development of time legend, so that time presentation suits the nature of the depicted theme. Consecutively, this general pattern is applied to a set of pieces of cartographical work depicting (historical) battles. The tangible result of this work is an interactive cartographic animation presenting the battle of Gettysburg and preceding events. Keywords: time, cartography, temporal legend, animated map, battle
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The role of microRNAs in chronic lymphocytic leukemia
Moravec, Martin ; Macůrková, Marie (referee) ; Hájek, Miroslav (advisor)
2 Abstract MicroRNAs (miRs, miRNAs) are recently discovered molecules (19-25 nucleotides long) that regulate gene expression at post-transcriptional level by either blocking protein synthesis or mRNA degradation. As a part of gene silencing mechanism, miRNAs are involved in cellular processes, such as apoptosis, cell proliferation, development and viral defence. miRNAs have been intensely studied in connection to disease pathogenesis. Chronic lymphocytic leukemia (CLL) is the most common leukemia in Western countries affecting mostly elderly people. In my work I focus on explanation of miRNA functions and their contributions to chronic lymphocytic leukemia (CLL). I describe previously published data about miRNA-15, miRNA-16, miRNA-143, miRNA-145 and miRNA-155 in connection to this disease. Based on recent reports, I also discuss the potential role of miRNA-326 in CLL pathogenesis.
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