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Characterization and identification of wheat allergens from foodstuffs containing wheat
Goliáš, Jaroslav ; Prokešová, Ludmila (referee) ; Tučková, Ludmila (advisor)
In some cases, ordinarily accepted food can evoke harmful clinical symptoms which are brought on by sensitivity on specific component of foodstuff. This harmful reaction on accepted food is called food allergy and it tends to go up in the world's population in last years. The food allergy affects especially children as well as adults. The important role in the food allergy plays wheat and foodstuff containing components of wheat. Wheat allergens from flour is already well-known, but man accepts wheat in food in the treated form (heat treated food) what can decrease or increase allergenicity. Moreover, enzymes presented in gastrointestinal tract of man can influence allergenicity of wheat allergens too. The goal of this thesis is characterization of allergens presented in heat treated wheat foodstuff and in wheat foodstuff digested by enzymes in gastrointestinal tract of man. Characterization of these altered and newly produced allergens can help find better diagnostic techniques, increase specificity of detection of IgE antibodies, enable pointed therapy of allergic patients or find and apply new and suitable diet.
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Expression of SWC7 molecules on the pig B lymphocytes.
Fikrová, Petra ; Konečná, Klára (referee) ; Jílek, Petr (advisor)
The aim of this graduation thesis is to clarify the expression profile of the SWC7 molecule on B cells in swine and eventually to resolve its function during maturation. At present it is possible to define the maturation pathways of porcine B lymphocytes in the periphery on the base of the expression CD2 and CD21 cell surface molecules. The flow cytometry was chosen as a central experimental method for detection of B lymphocytes carrying SWC7 molecule on their surface. The ability of individual B cell subpopulation to produce antibodies was also measured by ELISA. Principal phenotype characterization showed that the SWC7 molecule is found only on the surface of IgM+ and CD21+ B cells. These may be CD2 positive or negative. Based on this finding, the individual subpopulations of IgM+ CD21+ B cells differing in expression of SWC7 and CD2 molecules were purified by flow cytometry. Subpopulations were thereafter cultivated and subsequently analyzed for alterations in their phenotype. Media from cultivated cells were also analyzed for presence of porcine IgM, IgG and IgA antibodies by ELISA. Results indicate that the expression of SWC7 molecule on the surface of B cells may be considered as a maturation marker because loss of SWC7 molecules from the cell surface is irreversible and because only SWC7- B cells are...
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Transformation textile company Fezko in Strakonice in 19th and 21st century
Vlčková, Gabriela ; Jakubec, Ivan (advisor) ; Skřivan, Aleš (referee)
The Bachelor's Thesis deals with the south-Bohemian textile company Fezko situated in the city of Strakonice. After founding in the beginning of the 19th century, the company was in progress and changed differently over the years. It became famous mainly as a manufacturer and a supplier of fezzes, a kind of red headgear worn largely by Islamists. Fez was exported to distant places, especially in Turkey. The company was not only specialised in fez production, but there were also made other headgears, such as berets, and common pieces of clothing, such as coats, suits and scarfs. At present, the company focuses on the manufacturing of textile interiors for the automotive industry. In the thesis, there are also stated the reasons why fezzes were produced in the city of Strakonice and its surroundings. Last but not least, the thesis explores the life conditions of company workers, their wages, working time, in what way the company influenced their lives and, finally, the possible reasons why the workers went on strike.
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Implication of ligninolytic enzymes produced by immobilized white-rot fungi in decolorization of synthetic dyes
Šušla, Martin ; Novotný, Čeněk (advisor) ; Gaper, Jan (referee) ; Masák, Jan (referee)
6 Abstract The aim of this Ph.D. thesis was to characterize ligninolytic activities of two white- rot fungi, Dichomitus squalens and Irpex lacteus, cultivated on various solid supports in their immobilized form and to identify the role of ligninolytic enzymes in the decolorization of synthetic dyes. Significant induction of laccase activity was observed in D. squalens pine wood cultures when compared to liquid cultures. The induction of laccase was probably due to the presence of phenols, extractable from wood with organic solvents. Two laccase isoforms, Lc1 and Lc2, were isolated from the culture liquid using FPLC. The enzymes revealed identical molecular masses of 68 kDa, however, they differed in isoelectric points and their dye decolorization capacity. The decolorization of the azo dye RO16 by Lc1 in the presence of redox mediator was followed by a regressive colorization of the sample when incubated for 72 h. Conversely, no backward trend was observed during RO16 decolorization using Lc2. In contrast to D. squalens, I. lacteus immobilized on pine wood cubes produced a low level of manganese peroxidases and no laccase induction was observed. Both fungi produced MnP as a dominant enzyme in solid state wheat straw cultures. D. squalens MnP obtained from the wheat straw cultures was able to decolorize...
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Implications of coffee trade for economic development of Ethiopia
Svobodová, Kateřina ; Adamcová, Lenka (advisor) ; Borgström Korba, Anna (referee)
This diploma thesis deals with the development of world coffee market and impacts for economic development of Ethiopia. First part is focused on characteristics of and trends in the world coffee market in terms of cultivation demands, world production, exports and domestic consumption in coffee growing regions. The analysis of world coffee prices, consequences and potential solutions of the global coffee crisis builds the core of the first part. The second part continues with analysis of economic development and economic structure of Ethiopia, from which follows the enormous economic importance of coffee for this poor country. Further, the coffe market and its developments is characterized - as far as produced and exported volume and prices paid to producers in the country are concerned. In conclusion of the second part the implications of Ethiopian development strategies into coffee sector are examined.
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Forming the "cosmoworld" and finding its essence
Horáková, Klára ; Šmídová, Olga (advisor) ; Petrusek, Miloslav (referee)
This dissertation thesis on the issue "How Cosmoworld Forms and What It Is" deals with the formation and content of a magazine for young women named Cosmopolitan. The aim of this work is to ascertain, whether the type of the woman constructed by this magazine is the emancipated one, to uncover how the magazine defines itself or, put more aptly, how it helps to maintain traditional stereotypes attributed to womanhood. In the introduction, the author pursues the basic theoretical concept and methodological tools suitable to apply in analysing media like Cosmopolitan. The very research into the process of production of the magazine and its content is covered in the second part of the thesis. Using the means of discursive analysis, the author attempts to resolve the type of womanhood re/constructed by the magazine and to work out what are the backdrops or frames which the magazine sets womanhood in. Further, we see the author researching the very creation of the magazine (the ethnomethodological observation in the editorial office), identifying the rules followed in writing articles for Cosmopolitan. Having analysed the content of Cosmopolitan and the process of its production, the author has come to the conclusion that Cosmopolitan re/produces a woman who sees her liberation and emancipation in sexual freedom...
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Flow Parameters Simulation Technique in Annular Turbine Cascade INLET
Jelínek, T. ; Němec, M. ; Uruba, Václav
This article summarizes the concept of equipment which produces the required flow parameters at the inlet of annular turbine cascade. The investigation of inlet flow parameters - the boundary layer on hub and tip inlet channel end-walls and the inlet flow turbulence are part of a project intent on a flow in multistage steam turbine arrangement. This variable inlet can produce the laminar or turbulent boundary layer, respectively the laminar or fully turbulent velocity profile in the inlet channel. The change of inlet turbulence level is controlled by the grid. The detailed measurement of inlet flow: the turbulence and the total pressure distribution across the height of inlet channel were performed by HWA (hot wire anemometry) probe and by total pressure probe, respectively. The measurement results characterize the inlet flow for several inlet channel arrangements and are used for the experimental investigation of the flow field inside the stage and as the inlet parameters for CFD simulations.
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Some differences in the biology and behavior of the species eastern gorilla (Gorilla beringei) and western gorilla (Gorilla gorilla)
Kotýnková, Nicola ; Kracíková, Olga (advisor) ; Tománek, Pavel (referee)
Genus Gorilla is divided into two species includes four subspecies and one subspecies unrecognized.
Gorillas are the largest living apes and and eight million years ago, as a line, they separated themselves from family Hominidae.
These populations are found in nine African countries and they are separated by forest Congo.
Their most developer sense is vision. The body is adapted for knuckle-walking and their limbs are adapted to grip, with moveable arms and long arms. They have a mighty body with a robust skull and teeth with bumps for processing a hard food.
Gorillas excel significant sexual dimorphism, which is characterized by variously sized body.
Thanks reproduction arises extensive diversity within species in different strategies that help increase fitness. Dominant males mate with sexually mature females, while males with lower status mate with younger females because they are not fertile yet. Compared to that family groups have only one silverback male with which the female mate.
There are substantial differences in eating habits between gorillas. Dietary differences between species and subspecies are caused mainly by food availability. Gorillas are considered for herbivorous and frugivorous animals.
Gorilla groups are polygenic. With one dominant silverback male a few females and their descendants, they form a cohesive group.
They create nest for sleeping every night which different in location and stage of construction.
They communicate with each other through the auditory, visual and chemical signals. They use approximately 102 types of gestures and 17 types of audio signals to communicate.
In wild gorillas using tools is scarce, therefore mainly used to get food from difficult sources most of its body. While gorillas in captivity use tools successfully.
To protect against predators, the female consort with males who protect them.
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Development and further education of teachers in DDM
ŠULÁKOVÁ, Tereza
This thesis deals with Development and further education of leisure time pedagogues in youth Centres. This thesis aims to describe which development oppourtunities are and valorize importace of further education for leisure time pedagogues professional develompent. The work is devided to four main chapters. The first chpater characterizes th youth center. Second one describes leisure time pedagogues. The third one is about leisure time pedagogues direkt in youth center. And the last one deals with development oppourtunities, accredited courses in further education systém, and another "new" further ecucation oppourtunities.
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Routing protocols in scale-free topology networks
Mahdal, Ondřej ; Kyselý, František (referee) ; Skořepa, Michal (advisor)
This master's thesis (further only MT) deal with problems of scale-free topology networks with a view to technology MANET (Mobile Ad hoc network) or more precisely Motorola MESH. Further studies routing technique and quality of services (QoS) in these networks. Theoretic part of MT in introduction shortly characterizes properties and kind of Ad hoc networks, next part is dedicated to technology Motorola MESH and shows practical usage of this technology. Analysis of problems quality of services and optimalization QoS in MANET is content third parts of MT. Fourth part specifies division of routing protocols, analysis of routing in MANET and characteristics routing protocols OLSR, AODV, DSR and TORA. In practical fifth part of MT are in introduction simulated simple MANET and mesh networks and further is created model of MANET network, into the model are implemented accessible routing protocols in simulation program OPNET Modeler and on the basis of simulation these protocols or more precisely output statisticians of simulation are protocols compared from several aspects and suitability for transmission of multimedia data (voice, video).
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