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Multilayer hierarchical models
Béger, Michal ; Petříčková, Zuzana (referee) ; Štanclová, Jana (advisor)
This diploma thesis deals with hierarchical associative memories (HAM), which have been experimentally analysed only in the case of two layer hierarchy so far. The aim of this thesis is to study existing hierarchical models and evaluate experimentally their performance for more than two layer hierarchy. We show, that existing hierarchical model HAM is not suitable for three or more layer hierarchy. For that reason, we propose a new version of hierarchical model (called HAM-N), which enables utilization of any number of layers. The new model HAM-N uses the structure of the HAM model. However, due to modied learning and recall process, the HAM-N model eliminates the above-mentioned drawbacks of the HAM model. Finally, the HAM-N model is experimentally studied with respect to processing of large amounts of correlated patterns. Thesis also includes analysis of experiment results.
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The essential processes of FeS cluster assembly and mitochondrial protein import in parasitic protists
Šmíd, Ondřej ; Tachezy, Jan (advisor) ; Hampl, Vladimír (referee) ; DYALL, Sabrina D. (referee)
CONCLUSIONS Mitochondria appear to be vital organelles for all eukaryotic organisms known to date. A large body of evidence strongly indicates that hydrogenosomes and mitosomes, the organelles of anaerobic or intracellular parasitic protists lacking the "typical" mitochondria, are only a variation on the mitochondrial theme. However, even though the three organelles are evolutionarily the same entity, remarkable differences exist between them that reflect the adaptation of the organisms to their specific niches. In the thesis I tried to unravel some aspects of the essential mitochondrial processes of FeS cluster assembly and mitochondrial protein import in parasitic protists T. brucei and G. intestinalis. We investigated the function of the T. brucei cysteine desulfurase IscS and the scaffold protein IscU. We demonstrated that the two proteins are essential for FeS cluster formation and consequently the viability of the procyclic stage of T. brucei. Even though both IscS and IscU were specifically localized to the mitochondrion, their deficient expression affected the maturation of FeS proteins operating not only in the mitochondrion, but also in the cytosol. This indicates that a crucial part of FeS cluster assembly is localized to the mitochondrion of T. brucei. One of the major differences between the T....
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Analysis of primary photosynthetic processes in conifers: A comparison of selected methods and their possible utilisation for the study of genetic variability
Palovská, Markéta ; Holá, Dana (advisor) ; Albrechtová, Jana (referee)
Conifers are important both ecologically and socioeconomically, however, same parts of their biology are not that well researched. This includes genetics and breeding and partly even physiology. Because quantitative genetic analyzes applied in breeding necessitate an analysis of a large number of samples, and conventional methods of analysis are quite time-consuming, certain parameters describing e.g. the activity of photosynthetic electron-transport chain (ETC) are considered for such use. Several methods of the measurement of the activity of photosynthetic ETC exist, but there are some problems with their usage in conifers. I studied this issue from different points of view in three parts of this thesis. 1) I compared the photosynthetic ETC activity in 8 species of conifers using chlorophyll (Chl) fluorescence measurements on intact needles and polarographic measurements in isolated chloroplasts. Each method brought different information. 2) I measured Chl fluorescence parameters, reflectance spectra and pigment content in 536 genetically defined trees of Pinus sylvestris L. Many parameters showed relatively high genetic variability and heritability. I have also determined the suitability of various reflectance indices to estimate pigment and water content of needles. 3) I have optimized the...
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The problematic aspects of financing of cities and minucipalities in the Czech Republic.
Kruntorádová, Ilona ; Jüptner, Petr (advisor) ; Čopík, Jan (referee)
In 2000, respectively 2003 the Czech Republic finished the first and the second stage of public administration reform, which is closely linked with the issue of public financing, especially the financing of municipalities. This diploma thesis analyzes the problematic aspects of the financing of municipalities in the Czech Republic in terms of the status of municipalities in the political system. Municipal budgets are the means by which local governments fulfil their objectives, policies and ensure the development of their municipalities. It is a highly topical issue. Political leaders of local authorities are not due to play the differing roles and provide different level of public services, united in the opinion of the form of financing of local budgets. Municipalities do not have such a strong unified voice in ensuring its priorities. These different interests are reflected in the programs of political parties. The question of financing municipalities would probably disintegrated ODS and TOP 09 together with STAN in the coalition in cas of the absence of other contradictions. The issue of local governement funding goes beyond the scope of political science. It is multidisciplinary, interdisciplinary topic. In order to grasp and research the topic from political science perspective, it is...
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Heart Rate Response in Newborns to Relatively Strong and Mild Trigeminal Odorants
Boušová, Jiřina ; Martinec Nováková, Lenka (advisor) ; Brothánková, Pavlína (referee)
The widely accepted view nowadays is that experiencing odours as rather pleasant or unpleasant is, to a certain degree, shaped on a daily basis through individual experience within one's culture via evaluative conditioning or, rather marginally so, via mere exposure to that certain odour. In other words, humans are not born with any fixed set of olfactory likes or dislikes but rather, they acquire them throughout their lifetime. However, olfactory sensation is not a "pure" percept, as odorant stimuli generally elicit a qualitative percept of an odorant - generated mainly by the olfactory nerve - as well as some degree of chemesthesis - a tactile confound of the odour generated mainly by the trigeminal nerve. The olfactory and trigeminal system exhibit complex interactions at both the peripheral and central level of chemosensory processing, which is also reflected in perceptual characteristics of the final percept, including perceived pleasantness (hedonics). If the olfactory contribution alone does not easily predict neonatal odour hedonics, due to newborns' limited previous exposure to chemosensory inputs, one may hypothesize that together with the strength of the trigeminal contribution they may form a significant factor affecting neonatal appetitive/aversive responses to odours. In the present...
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Formation of spatio-temporal molecular gradients in early embryonic development of Xenopus laevis.
Šídová, Monika ; Tlapáková, Tereza (advisor) ; Pěknicová, Jana (referee) ; Petr, Jaroslav (referee)
Clarifying the underlying spatio-temporal mechanisms that determine body pattern is important for detailed understanding of embryonic development. A crucial question of vertebrate embryogenesis remains: when and how are single blastomeres determined for differentiation that subsequently leads to body axes specification and the formation of different tissues and organs? The answer to this question will be beneficial for primary research as well as in the field of applied medicine. The main aim of the presented thesis was to study spatio-temporal molecular gradients of cell fate determinants during early embryonic development. The African clawed frog Xenopus laevis was used as a model organism because of their large size of oocytes and external embryonic development. Due to late activation of embryonic transcription, a crucial mechanism of early blastomeres determination is dependent on asymmetric localization of maternal factors within oocyte and their uneven distribution into single blastomeres during early cell division. Two main localization patterns were identified along the animal-vegetal axis of the mature Xenopus oocyte using qPCR tomography. The localization gradient with preference in either animal or vegetal hemisphere was found for maternal mRNA as well as miRNAs. Moreover, two vegetal...
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The Specific of Nursing Care in Patient Undergoing the Examination of Bone Marrow
ČOUDKOVÁ, Marie
Haematology is a medical field. It is the study ofblood cells. It studies blood cells from the point of their morphology, quantity and function. The clinical haematology focuses on the irregularities in a patient´s blood and studies how the irregularitiesdiffer from the patient´s normal physiological condition. Due to this work haematology can help with diagnosis of other diseases. In haematology many investigative methods are used. One of them is aspiration and biopsy of bone marrow which is a hematopoietic tissue daily producing a large number of blood cells. Unless the formation of blood cells is sufficient enough, the disabled person may be at risk of their life. When the function of bone marrow is affected the patient may be impinged by serious infections, anemia risk or bleeding tendency. Bone marrow is examined for suspicion of haematological or oncology illnesses. Therefore, it has a very important rolein diagnosis of these. It is performed bya prolonged fever, mainly among the people who have a bad immune system. For blood collection are used investigative methodswhich consist ofaspiration and percutaneous bone marrow biopsy. These physical examinations provide some pieces of information about the condition and the ability of the blood cells´ production of bone marrow. Very important part of this physical examination is a specific nursing care. This bachelor´s thesis is focused on the specific nursing care of the patients who undergo the examinations of bone marrow. This thesis mainly includes the methods of bone marrow harvest, how the patients are informed about the examination of bone marrow,the patients´ nursing care after the operation and the role of a nurse at the haematological ward. The introduction of the theoretical part involves physiology of bone marrow and the study of haematology. The other parts discuss indication for bone marrow examination, haematological and oncological illnesses, bone marrow aspiration or percutaneous biopsy of bone marrow. The large part of the thesis deals with bone marrow aspiration, the process of the operation and the nursing care. It also focuses on percutaneous biopsy of marrow bone where the meaning of the examination and the process of bone marrow harvest operation are explained. At the end of the theoretical part a collection of hematopoietic stem cells from particular peripheral blood in mentioned. The aim of the bachelor thesis was to find out certain specifics of nursing care of the patients who undergo the examination of bone marrow. The following research questions were selected: What are the specifics of nursing care of the patients who undergo trepanobiopsy? What are the specifics of the patients who undergo aspiration (sternal puncture)? What are a preparation and assistance of a nurse during the examination of bone marrow? Which roles does the nurse have during the examination of bone marrow? How are the patients informed about the bone marrowexamination? The practical part includes results from a quantitative survey which was a semi-structured interview. The resulting interviews were written and then were processed by a certified coding technique. The research group comprised six nurses working in the haematological ward and six patients who have undergone the examination of bone marrow. Regarding the gathered results, an information brochure was put forward as a part of the thesis (see Annex number 5) and was oriented especially at patients undergoing the bone marrow examination. The brochure will be proposed for practical use at haematological wards.
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Integration of clonal plants in heterogeneous environment
Vinšová, Jana ; Weiser, Martin (advisor) ; Macek, Petr (referee)
Clonal plants may be able to cope with spatial and temporal heterogeneity due to the physiological integration of ramets. To begin with, I discuss basic characteristics of clonal plants and then I provide a review of theoretical and empirical work dealing with the advantages and disadvantages of clonal growth in heterogenous conditions. Three main traits - translocation of resources, division of labour and foraging - are examined in terms of their contribution to the success of clonal plants. Despite a large amount of attention which this topic has received in the past few decades, a number of issues still remain unclear.
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Blaze Gratings with a Ribbed Back Slope
Krátký, Stanislav ; Meluzín, Petr ; Horáček, Miroslav ; Kolařík, Vladimír ; Matějka, Milan ; Chlumská, Jana ; Král, Stanislav
Binary relief phase-modulated gratings provide symmetrical diffraction of the incoming light beam. Asymmetrical gratings, e.g. asymmetrical triangular blazed gratings, are characteristic by an asymmetrical diffraction behavior, where one of the first diffraction orders is more important than the other one. Electron beam lithography is a suitable and flexible tool for patterning of such kind of gratings. High quality results can be readily obtained when the period of the grating is relatively large and the relief depth is relatively low, this is the case of gratings with a small blaze angle. As the blaze angle increases, the quality of result suffers from several patterning-related issues. One of the problems is a reflection of the incoming light beam from the back slope (anti-blaze facet) of the blaze grating. We propose a novel configuration, with a ribbed modulation of the back slope. This modulation is perpendicular to the direction of the grating grooves. This paper presents an analysis of the proposed blazed grating configuration. E-beam pattern generators were used to prepare a few\nsamples of blaze gratings with a ribbed back slope. One part of the experiment was performed with a Gaussianshaped beam and another one with the variable-shaped beam. Results of the experiment are presented.\nFinally, we discuss the optical performance of two blaze gratings with similar parameters, one of them is with the flat back slope and another one is with the ribbed back slope.
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The appraisal of development of tax proportion of budgetary incomes of municipalities and regions in the Czech Republic
Poláková, Tereza ; Peková, Jitka (advisor) ; Krbová, Jana (referee)
The aim of this diploma thesis is a detailed analysis of tax incomes of municipalities and regions with focus on four particular municipalities and one region in a practical part. From the year 2001 to 2015 the share of tax incomes and their development are evaluated as a part of total incomes. The theme is highly topical due to ongoing discussions among lawmakers on the subject of budgetary allocation of taxes. The law determining the budgetary allocation of taxes is modified frequently, that is why knowing the impact of changes on particular municipalities or regions is helpful. This knowledge can be useful base for planning new adjustments of budgetary allocation of taxes. In the theoretical part there are mentioned expert opinions on financial management of municipalities and regions that arise from their functions and positions. The emphasis is put on incomes of municipalities and regions, especially on tax incomes. Particular changes from 2001 to 2015 in law regulating budgetary allocation of taxes are described here with their impacts on municipalities and regions. Incomes and management of selected municipalities representing various size categories and Liberec region are analysed in the practical part. Financial self-sufficiency is evaluated here. In general municipalities are more financial self-sufficient than regions. This thesis is further dealing with individual tax incomes and impacts of changes in budgetary allocation of taxes on their size. Making models of theoretical incomes is used to simulate effects of diverse law adjustments. Results of the analysis show that tax incomes of municipalities and regions are generally increasing. During the observed period there was tendency to lower the growth of tax incomes of large cities, to increase the growth of tax incomes of small municipalities and to straighten the level of tax incomes of medium municipalities thanks to changes in budgetary allocation of taxes. Tax incomes of regions were increased thanks to changes in budgetary allocation of taxes despite of partial decrease in particular segments of tax incomes after 2012.
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