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Lucifensin, a peptide behind the maggot therapy
Čeřovský, Václav
The larvae of the green bottle fly (Lucilia sericata) are routinely used as a medication for the treatment of necrotic, non-healing and highly infected wound in many hospitals all over the world. We have identified and determined primary structure of the defensin originated from the immune system of Lucilia sericata larvae and named it lucifensin. This is 40 amino acid residues and three intramolecular disulfide bridges peptide. The synthetic lucifensin was active against Gram-positive bacteria and was not hemolytic. We studied the importance of lucifensin disulfide bridges and the N-terminal part of its molecule for the structure and for antimicrobial activity.
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Study of plasmachemical reduction of corrosive layers on bronze
Miková, Petra ; Selucká, Alena (referee) ; Krčma, František (advisor)
One of the important stages of the human history is certainly Bronze Age, on our territory dated 1900 - 800 BC. At that times, it was produced many objects of bronze, such as swords, spears, daggers, ornaments, jewelry. These artifacts are now found by archaeologists in excavations around the whole world. During the years there was an exposure to various types of substances and the environment and result in extensive corrosion of the used materials. To better understand the culture and traditions of our ancestors, it is necessary to carefully eliminate the corrosion and thorough inspection of these subjects. In this work, we consider the model corrosion layers removal by reduction in low-temperature hydrogen plasma. Plasma chemical reduction method was developed during the eighties years of last century and currently is further rapidly improved. Unfortunately, the process mechanism is not yet precisely known. Preparation of model corrosion layers were as follows: bronze blocks (weight approximately 80 g) were sharpend by electrical grinding machine (used paper 280 and then 600). Thus treated samples were rinsed in ethanol, dryed by hot dryer and stored in plastic bags. A Petri dish was placed at the bottom of the desiccator and it was poured by 20 ml of concentrated hydrochloric acid. Above the bowl has punched ceramic grid, on which the samples were placed. Closed desiccator was placed in darkness at the ambient laboratory temperature. To accelerate the samples corrosion process in a desiccator, the samples were sprinkled with acid. Plasma chemical treatment was carried out in a cylindrical reactor of quartz glass with outer copper electrodes. The RF (13.54 MHz) capacitivelly coupled plasma was used for the samples treatment. For each sample have been selected specific conditions: power (50 - 300 W), pulse (duty cycle of 10 – 25 %) or continuous mode. The optical emission spectroscopy monitored dependence of OH- intensity during the reduction. The decrease to the 1/10 of the maximum value leads to end the experiment. Based on this criterion, the treatment time of samples was in the range of 30 - 80 minutes. Corrosion has been removed from 7 samples. All the samples were covered by corrosion deep green color with a clearly visible crystalline structure before their inserting into the reactor. After the treatment, the color was dark black, and after leaving the air began to surface green, in some samples was observed in white and yellow tint. Surface top layer was removed on samples 1 and 5, where spontaneous dropout was reached. The pulse mode with duty cycle of 10 % and the powers of 200 and 300 W were applied for these two samples. The surface layer of samples treated with pulsed mode of 25 % was easily removable by spatula. The work demonstrated the applicability of hydrogen RF plasma in pulsed mode for the corrosion removal from bronze samples. The further work will be focused on the optimal treatment conditions search.
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Historic greenhouse in castle grounds Konopiště
Nohejlová, Kristýna ; Česká, Jana (advisor) ; Martin, Martin (referee)
The aim of this bachelor thesis is an inventory of all plant kinds in a historical green house Konopiste. It is known that there are over 200 different kinds of plants currently growing in that place. The exact number cannot be listed and stated, because it is still moving and varies. There are some plants that are still growing at one place in a soil for many years. One of the examples is Phoenix canariensis, which dominates the whole green house. The majority of plants are grown there in planters. That enables their easy manipulation within the whole green house. The inventory of all kinds of plants took place between March 2015 and February 2016. Each plant has been noted down according to its station.
The first chapter deals not only with the history of the castle itself, but is also described together with the history of the green house and its surrounding areas. Unfortunately there has been only a limited amount of resources available to study for me and to use. Therefore my description does not go into deep details. In this chapter, I therefore describe some technical achievements and economical parameter and measures. They show how the green house has been heated in those days and describe the details of pluming. There is also a short description of a mechanism which opened the windows in order to cause air flow circulation.
The main was Encyclopedia of garden plants by Christopher Brickell. The Latin names of individual kinds were flowingly sorted using the websites ipni.org.
The fifth chapter is dedicated to the inventory. Each green house section has its own general description, from which, there has been lately chosen a sample of the most interesting plants. Their characteristics were then described, using those specialized books. The final inventory of green houses is than shown in additional tables.
The sixth chapter includes and deals with recommendations of the writer. The writer suggested a better marking, using a good quality materials and labels. (Steel and enamel) Another suggestion, which the writer made, was to follow the inventory stock take, but some of the plants should be repositioned. The ones, that were not in blossom, or were not grown up enough, in order to mark their characteristics. In an attachment there are the photographs of the castle, together with the Rose garden and its green house. Other photographs show some interesting, or rare plants. They may have unusual shape, or a beautiful loom.
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Potential of garden and productive urban space in the today's city
Adamková, Jana
Green areas were an integral part of cities throughout the history. Productive garden, which represents an archetypal symbol of human livelihoods and connection between man and earth, has been disappearing from the urban structure in recent years. Urban agglomerations and their resource uses become the dominant feature of the human presence on the earth, profoundly changing humanity’s relationship to its host planet and its ecosystems. Cities require vast areas of land for their sustenance and have come to depend on large amounts of food being brought in from outside the land area they actually occupy. The article deals with the importance of gardens for humans and highlights the role of productive urban space in process of creating a viable urban environment. As the world urbanises, greater local food self-reliance, using nutrients accumulating in our cities, must be regarded as an important aspect of sustainable urban development. Along with other initiatives and activities, urban agriculture therefore has an important role to contribute to the sustainability of cities in various ways – spatially, socio-economically and environmentally.
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Architecture of Havířov in the Era of Socialist Realism
Sedláčková, Jana ; Novotná, Eva (advisor) ; Schmelzová, Radoslava (referee)
Short annotation Architecture of Havířov in the Era of Socialist Realism Building of Havířov was part of complex Nová Ostrava. Nová Ostrava was key project of first five-year plan. There was used insufficiently explained method of socialist-realism, which was from SSSR. Important for sympathy of formation Havířov is poltical and cultural development of ČSR after Second world war. Important is explanation of idea and platform of the new method and comparing of examples in architecture and art. Base of this work is a analysis of execution of a project method of socialist realism in Havířov. Firstly in this part is a history in ostrava's region, because this progress is connected with coal fields. Next are history of building Havířov, analysis and comparing of town planning, architecture and art. Connection of urbanism, architecture and art made synthesis for re-education of people. The best for rating of new town, which is built on green field, is survey of contemporary of inhabitants. Socialist realism's architecture in Havířov grew up at bad time, but this architecture is pleasant place for life, has mainly human dimension, public lawns and diversity. Keywords: socialist realism, architecture, urbanism, art, Havířov
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Conversion relating to vacant historical buildings of the castle Žďár nad Sázavou
Dzianová, Hana ; Zemánek, Václav (referee) ; Zemánková, Helena (advisor)
The area is located in the southern part of the castle complex in Zdar, land in an urban zone. The original function of the castle in Zdar was a Cistercian monastery, founded 02.05.1252, which is credited with the expansion of the region. The castle is close to important UNESCO World Heritage Site - Pilgrimage church of the John of Nepomuk on the Green hill, which is a prerequisite for tourism in the region. The architectural design is based on the idea of recovery of unused buildings for the benefit of tourism on the one hand and on the other hand, it seeks to enrich local communities and the region. Suggest a feature builds on the newly created Museum of the new generation, which represents the potential of tourism. The draft proposal seeks to complete the missing and necessary functions in the area while trying to build on the tradition and the original object function.
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Study of photosynthetic response of green algae on presence of selected heavy metals
NOVÁK, Jan
With the world population growth, more and more agricultural areas fall into regions affected by waste products. Therefore it is important to determine their impact on environment toxicity. The aim of this thesis is to evaluate the effect of selected ions of heavy metals (CoSO4, CuSO4, CdSO4, ZnSO4 and K2Cr2O7) on the process of photosynthesis in a selected green algae species (Scenedesmus quadricauda) by means of monitoring of dynamics of oxygen release into solution of nutrient medium. The experiments with individual compounds proceeded by pH 7, temperature 25°C and light 450 ?mol m-2 s-1. Algareact device developed by the company BVT Technologies was used for measuring. Comparison with growth curves Scenedesmus quadricauda by different pH was used for the validation of the method. The method of the measurement of dissolved oxygen in medium is suitable for the measurement of immediate impact of chemical compounds on photosynthetic activity of examined organism. The method is restricted by the fluidity of measured solution. The lowest quantity of oxygen disolved inmedium was measured by CdSO4 in concentration 0,1 M.
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Green ICT analysis in IT companies operating on the czech market
Targa, Jan ; Basl, Josef (advisor) ; Gála, Libor (referee)
Recent economical crises, constant development of new technologies and faster globalisation cause different changes in all organisations all over the world. One of these changes are also different considerations about suistainable development and the impact different companies have. Those organisations are getting more and more involved into this topic and become more competitive within quickly developing global economics. They look for the ways how to contribute to suistainable development. One of the ways is also suistainable IT in a company focused on Green ICT. The aim of this thesis is to describe Green ICT, how it matches with suistainable development and what are the areas it covers. Firstly, the thesis contains an explanation of what Green ICT is. Secondly it focuses on the approach to this issue in different companies of different sizes in different industries. To achieve this aim, there are used different surveys from worldwide known companies. In the next part this thesis deals with two most important global IT companies, that significantly influence supply of ERP systems to the Czech market. This part specifies the areas covered by above mentioned companies and their main achievements. The main goal of this thesis is to analyze current state of Green ICT within local market. To achieve this goal, there was taken a survey focusing on Green ICT perception at ERP suppliers on the Czech market. Data from this survey were then analysed and evaluated. This thesis also provides a measuring scale for evaluation of Green ICT level. To demonstrate this, there were two global companies evaluated compared to three local companies evaluation.
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