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The study of azaphthalocyanines interactions with unilamellar liposomes I.
Kučerová, Helena ; Zimčík, Petr (advisor) ; Miletín, Miroslav (referee)
Three types of different octasubstituted zinc azaphthalocyanines (ZIP-44Zn, ZIP-21Zn and ZIP-65Zn) were incorporated into liposomes made from dioleoylphosphatidylcholine (DOPC). These dyes were incorporated into LUVETs (large unilamellar vesicles made by extrusion technique) which were obtained from suspension of multilamellar vesicles. Fluorescence spectra of the dyes in LUVETs were measured. The binding constants of azaphthalocyanines with LUVETs were calculated. Absorption spectra of the dyes in LUVETs were also measured and extinction coefficients were calculated. We found out that ZIP-44Zn (n-octylsulphanyl substituents) was present in DOPC liposomes only in very small amount while the other two dyes were incorporated in high concentration into LUVETs. This fact was connected with the level of aggregation of water insoluble AzaPc. ZIP-44Zn contains long alkyl chains as substituents on periphery. These substituents do not inhibit sufficiently the aggregation in water medium or in lipid bilayer. On the other hand, ZIP-21Zn and ZIP-65Zn contain bulky substituents on periphery and they were incorporated in large amount in phospholipid bilayer of liposomes.
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Daily Liquid Consumption among School-age Children
Pleskačová, Jana ; Stránský, Miroslav (advisor)
When studying the literature related to drinking regime , I found a series of lofty introductory sentences . They point out that life emerged from water , and that the human body is liquid consisting of approximately sixty percent. Already for these facts is obvious as sufficient fluid intake for the preservation of human life and health is important. Unfortunately, nowadays life goes faster and the large number of us do not have time to eat healthy and think adequate and regular payment of loss of fluids . In the children's body contained more fluid than the percentage in the body adult Moreover, children are given the greater weight of the body surface and the fluid are so relatively more are lost by evaporation. Daily turnover in the adult human consists of 6 % in Newborn 15% of the total volume of fluid in the body. The feeling of thirst in children weaker than adults. These are all reasons why we the drinking regime had children increasingly observed. The choice of this topic for the subject of the thesis led me was his importance in relation to human health.
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Modeling the recovery of anthropogenically acidified mountain waters
Hardekopf, David
Thesis Summary The five chapters of this thesis consist of manuscripts published in peer-reviewed journals, focused on the effects of anthropogenic acidification on freshwater ecosystems in the Czech Republic and Slovakia, and an attempt to understand the processes and time-frame of recovery using modeling. In the Tatra Mountains in Slovakia and the Bohemian Forest in the Czech Republic, high mountain lakes have been the focus of long-term studies on both the biological and chemical impacts of acidification. The effects of anthropogenic acidification on headwater streams in the mountains and highlands of the Czech Republic have been recognized more recently, and the resulting changes to the biological community and trends in chemical changes are less well- known. Dynamic modeling, combined with the incorporation of scenarios of possible climate change, is used to investigate the present status and future trends in recovery of these ecosystems after recent reductions in atmospheric acid deposition. The first chapter is a paper on the macroinvertebrate biota in atmospherically acidified streams in the Czech Republic. This paper shows the effect of low pH on the taxonomic composition in these streams. The second chapter is focused on the current (2004) chemical composition of 91 lakes in the Tatra Mountains,...
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Morphology of the Křížový potok river channel in the Eastern Krkonoše Mountains
Dvořáková, Magdalena ; Kliment, Zdeněk (referee) ; Engel, Zbyněk (advisor)
This master thesis deals with the morphology of mountain river channels - with structures, patterns and shapes resulting from fluvial processes. The findings are documented on the Křížový potok river channel in the Eastern Krkonoše mountains. There are several specifics which influence the evolution of mountain river channels. The first one is high elevation change and big slope of the river, which determinate the formation of typical structures, such as waterfalls, cascades and steps, often related with pools. Plane bed, riffle-pool and dune-ripple also represent typical structures of mountain river channels. Specific microstructures are represented by potholes. River channels in forested mountain basins are affected by logs and wood debris, which create local barriers where fine-grained material accumulates. In the Křížový potok river channel, a high diversity of the channel structures can be seen. The most common structures are step-pools and cascades. The stream is influenced by large accumulation of delluvial sediments and wood debris which create obstacles to the flow.
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Development of masonry systems with utilization in flood areas
Fabiánková, Aneta ; Sedlmajer, Martin (referee) ; Zach, Jiří (advisor)
Thesis describes the system of masonry structures in floodareas. It is based on liteature review of floods, mapping mansonry load during floods, dyinig after the passage of the flood wave solutions and water impermeability construction to prevent degradation and destruction. The main part is the design of the wall, which connectes hydrophobic individual elements of construction to provide resistence during floods. The diploma thesis includes practical verification of the functionality of a complete proposal to ensure the smallest possible water absorption caúitaly of the structure.
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Impact of internet on communal life in suburbia
Susová, Kateřina ; Ouředníček, Martin (advisor) ; Kostelecký, Tomáš (referee)
Thesis aims to describe the possibilities arising from the using of the Internet by residents of spatially defined suburban communities. Social environment in suburban areas is amended by arrival of large numbers of new residents, and any possibility of making new contacts becomes a potential benefit for community building and building of social capital in these rapidly changing areas. The work is focused on Internet forums of Prague suburb municipalities, which provide an opportunity for making new contacts between residents as well as participate in discussions on general issues with other locals and with representatives of local administration. Internet forums can significantly contribute to increase of civic or political engagement and the overall growth of social capital. Powered by TCPDF (www.tcpdf.org)
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The study oxidative stress markers in animal model of chronic colitis in large intestine.
Hocková, Erika ; Němečková, Ivana (referee) ; Nachtigal, Petr (advisor)
Crohn's disease and ulcerative colitis are both entities of inflammatory bowel disease (IBD). The nuclear transcription factor kappaB (NFκB) family consists of five different members, which are namely p65 (RelA), c-Rel, RelB, p50 and p52. NFκB has ability to promote the expression of various proinflammatory genes, to influence the course of mucosal inflammation and to mediate different cell-type specific effects. Bilirubin is an endogenous substance with anti-oxidative and anti-inflammatory effects, which influences the process of inflammation and has protective effect. This diploma thesis was aimed at conduction of methods of NFκB detection on paraffine tissue slices and observation of NFκB expression in large intestine walls of normobilirubin and hyperbilirubin rats (Gunn's variety) after chronic imunohistochemically- induced inflammation. Eight Gunn's variety rats (G+ ) and eight Wistar's variety rats (K+ ) were used in this study, all after long time administration of dextrane-sulfate (DSS). For seven following days, DSS (in concentration of 2%w/v) was added into drinkable water for these rats, followed by 14 days of clear drinkable water (without DSS) administered to rats. This three-weeks long cycle was repeated twice (3 cycles in total were completed). Six rats of both varieties (6 G+ and 6...
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Accumulation and biotransformation of nitramines in plant tissue
Šídlová, Petra ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Explosives are toxic, recalcitrant to degradation and contaminate large areas of land and ground water. Remediation of these xenobiotics is difficult and an enormous logistical task. Phytoremediation is a technique that offers an environment friendly, low-cost alternative to current remediation techniques. However this approach is hindered by the low metabolic abilities of plants towards these xenobiotic compounds and the phytotoxicity of these compounds. The fate of the explosives RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) and HMX (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine) on exposure to plant was investigated in hydroponic system of sunflower (Heliantus annuus L.) and hemp (Cannabis sativa L.) approximately for more than 10 days. Exposure levels were: 10mg/l and 50mg/l RDX, respectively HMX for sunflower; 25mg/l and 50mg/l RDX , 5mg/l and 50mg/l HMX for hemp. Their ecotoxicites were characterized by the test based on the inhibition of elongation of primary roots 3days-old mustard and hemp seedlings. Extracts of plants were analyzed by HPLC (liquid chromatography). These study showed that RDX and HMX was taken up by both plant species. The accumulation of these nitramines was observed more in the roots than in the aerial parts of the plants, except for RDX in hemp, where the...
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Sustainable production capabilities of territory, depending on climatic drought.
Skůpová, Jana ; Uhrová,, Jana (referee) ; Dumbrovský, Miroslav (advisor)
In this work, I dealt with the repercussion of drought on agriculture and land in the cadastral Jinošov. For this area, I analyzed the erosion and ratio of outflow. I also conducted a proposal of adaptation measures against erosion and water retention in the area. In conclusion, I analyzed withholding data and assessed the retention before and after the proposal of adaptation measures. The result of my work is finding that rainfall is inadequate and largely come out of the growing season, that despite all the measures fail to create on the necessary retention for the correct functioning of the landscape. Therefore it is necessary to solve water supply from other areas where is sufficient supply of water.
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Complex Scene Rendering on Mobile Devices
Matýšek, Michal ; Polok, Lukáš (referee) ; Kajan, Rudolf (advisor)
This thesis presents optimization techniques for efficient rendering of complex scenes on mobile devices. The introductory part of the text describes Unity game engine and the topic of mobile game development using this tool. Then follows a presentation of important optimization principles and methods for terrain rendering, large scale rendering of animated objects, rendering of animated water surfaces and of other elements in the scenes. The described methods include both general principles of optimization and specific optimization approaches based on the features of Unity game engine. The implementation of presented methods is described and used in practice in the context of mobile strategy game development.
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