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Reference gene selection for mRNA quantification in Haemonchus contortus
Růžičková, Michaela ; Matoušková, Petra (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Růžičková Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Reference gene selection for mRNA quantification in Haemonchus contortus Haemonchus contortus is a parasitic nematode, whose multiresistance to ant- helmintics means global problem, threatening mostly small ruminants farming. Genom and transcriptom have been published in 2013, allowing gene expression studies to be conducted. Reference genes for this studies have not been validated yet. Use of suitable reference genes is essential for accurate normalization of gene expression levels. Aim of this work was to identify and validate potential reference genes for gene expression studies in H. contortus adults. Eleven genes were chosen, stability of their expression was assessed in males and females of two H. contortus strains, one drug-susceptible (ISE) and one multi-drug-resistant (WR). Total RNA was extracted and reverse trancribed to cDNA. cDNA was diluted and analyzed using quantitative real-time PCR with SYBR Green I detection. Expression stability was evaluated by computer programs BestKeeper, geNorm, NormFinder and the comparative CT method. Ncbp, ama, sodc, gapdh and farb were found to be most stable...
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The Assessment in Vocational Training by Vocational Branch Hairdresser
RŮŽIČKOVÁ ZAUMÜLLEROVÁ, Věra
The aim of this study is to evaluate students within the teaching process in accordance with a specified timetable in relation to the SEP, not only in general, but also specifically as an assessment of a student by a teacher during the vocational training for hairdressers. This work aims to define the basic concepts and briefly describe the problems of student assessment not only by a teacher but also by the student himself, including the evaluation of the performance of one individual student by other students. Furthermore, it aims to describe the psychological aspects and patterns as a result of the evaluation, both on the part of the teacher, the student himself, classmates, but also on the part of participants involved in the evaluation process. The work will have a theoretical - empirical character. The theoretical part describes the evaluation of the student by the teacher during the educational training with a focus to the vocational training for hairdressers in accordance with SEP. It describes a method of systematic observation of the student within the scope of the evaluation process, potential analysis and interpretation of the performance shown by the student and forms of expression of the evaluated results. The work also aims to take advantage of student self-assessment and involvement of peers in the evaluation process. The theoretical part also pays attention to the very concept of "hairdresser", a historical perspective on the notion and not least the current view on this vocational training, its classification and description. The empirical part will propose an assessment system for educational training of the vocational field and will experimentally verify the possibility of its use in practice. In the context of the experiment, sheets will be proposed for self-assessment of students, for evaluation of student performance by classmates and for assessment of the students by teacher. These sheets will be used within one half-year for assessment during vocational training. Result of the experiment is validated through evaluation questionnaires for students.
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Vliv klíštěcích slin na fagocytózu borelií dendritickými buňkami
MARŠÁLKOVÁ, Eliška
In this study we examined the effect of the tick saliva from I. ricinus and the effect of recombinant protein IRS-2 from the saliva of I. ricinus on dendritic cells derived from the mice bone marrow. We studied their effect on the production of cytokines by dendritic cells after the stimulation by B. burgdorferi, their effect on the expression of genes, that participate in phagocytosis, and the impact of the tick saliva on phagocytosis of B. burgdorferi by dendritic cells.
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The effect of headphone and loudspeaker reproduction on classical music mix balance
Hadraba, Jakub ; ZIKMUND, Tomáš (advisor) ; SYROVÝ, Václav (referee)
Primary focus of the dissertation is comparison of loudspeaker and headphone reproduction and its influence on the mix balance of classical music recordings.
A listening test, which represents an essential part of the thesis, was performed on a test group of sound engineers who prepared mixes of selected recordings using both loudspea-kers and headphones. An analysis of the resulting mixes was performed to find out if any systematic changes could be detected between headphone and loudspeaker mixes. The results disclosed a systematic change in preference of reverberation. Individual approaches of the sound engineers are documented by an extensive graphical section of the thesis. Level of stability of the individual models has been examined using repeated testing on selected participants.
Selected headphone and loudspeaker mixes have been further evaluated by a listening group in a second listening test. Differences between the mixes were judged using both loudspeakers and headphones. Answers of the participants were analysed to see if there was an increased preference of headphone mixes when judged using headphones and vice versa. The results didn't show significant preference changes based on type of reproduction. The selected mixes weren't found to be "more optimal" when listened to on their respective reproduction device, nor could they be identified based on the subjective preference test.
A special part is devoted to evaluation of an internet-based survey featuring a statistical documentation of loudspeaker and headphone usage in todays music recording industry. Special look has been taken at the group of respondents who indicated classical music as primary music genre of their profession. Another theoretical part of the thesis covers the artistic and technical approach of sound engineers to classical music sound image creation, including commonly used technical resources and stylization methods.
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Legal consciousness of school-age children
Pazlarová, Hana ; Matoušek, Oldřich (advisor) ; Hartl, Pavel (referee)
Despite of the fact that the Czech Republic i s a developed country there are sti ll some challenges to be faced in childrens rights domain, mostly in involving children into everyday life of society connected with their participative rights. The thcoretical chapters analyze the childrens rights movement, and the implementing and monitoring ofthe Convcntion on the Rights ofthe Child both on the intemational and national Ieve!, with emphasis placed on Roma population in the Czech Republic. The research was focused on the lega! awareness of Czech school children with special consideration for Roma children as well. About one thousand children expressed their opinions and attitudes toward their rights.
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Nowcasting the Czech Trade Balance
Babecká Kucharčuková, Oxana ; Brůha, Jan
The Working Paper Series of the Czech National Bank (CNB) is intended to disseminate the results of the CNB’s research projects as well as the other research activities of both the staff of the CNB and collaborating outside contributors, including invited speakers. The Series aims to present original research contributions relevant to central banks. It is refereed internationally. The referee process is managed by the CNB Research Department. The working papers are circulated to stimulate discussion. The views expressed are those of the authors and do not necessarily reflect the official views of the CNB.
Fulltext:  PDF
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Characteristics of artistic expression in patients suffering from psychiatric illness
BAROŠOVÁ, Petra
The bachelor thesis is about the development and characteristics in theart work of five people with psychotic illness in the period of three years. The cases were collected in the psychiatric ward at the hospital in Tábor. The thesis consists of theoretical and practical parts. The theoretical part collects information about psychotic illnesses and a description of the therapeutic approach to the mentally ill. It also describes the visual artwork characteristics of psychotic individuals, the work with them and the efforts necessary from the art therapists' side. The practical part describes each patient. Observing the individual stories, approachesused in each case, their artwork, changes and progress in their development. There is a description of approaches used in the acute stage of the disease and after stabilization, the patients features in the artwork and the arttherapists influence.
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The analysis of landscape changes the influence comprehensive landscaping
ŠIMÁKOVÁ, Iveta
The purpose of this study was to analyse and asses the landscape changes on selected cadastral areas with completed comprehensive landscaping. For this purpose, two appropriate cadastral territory were selected, the cadastral area Borkovice and the cadastral area Myslkovice. The analysis was performed at three time periods - before the project the landscaping, in the project of landscaping and in the current state of the landscape. The changes in the landscape were assessed according to the state land use, road networks and territorial system of ecological stability. The results were processed into individual tables and graphs, providing information on the status of proposed and actually implemented elements.
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Statistical analysis of tourism in South Moravia
Pochobradská, Hana ; Grosz, Jan (advisor) ; Macháček, Otakar (referee)
The aim of this thesis is to use mathematical and statistical methods to analyze data relating to visitors of the South Moravia region. Data was collected via survey, which could participate respondents living in the Czech Republic. The theoretical part presents the basic concepts related to the theme of the thesis, such as tourism, visitors, tourists, excursionist and more. The thesis also includes the categorization of tourism from various perspectives, examples of organizations involved in tourism and especially familiarization with specific tourism destinations in the region. The practical part consists of an analysis of data collected and processed by the author. Furthermore, this section presents the results of a survey carried out by the author, who focused on attendance of the South Moravia region, the attractiveness of the region, most visited sites and financial possibilities of visitors of the region.
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Financial performance ranking
Čejka, Ladislav ; Neumaierová, Inka (advisor) ; Hájek, Jiří (referee)
The subject-matter of this diploma thesis is a comparison of financial performance of issuers of selected stock titles listed on the official market of the RM-SYSTÉM, česká burza cenných papírů a.s. stock exchange from a potential investor's perspective. The comprehensive comparison of financial performance presented both in the numerical and the graphic form is achieved through the application of the ranking method based on evaluation criteria analysing financial performance in the years 2009 to 2015 with a view of economic power, profitability, stability and investor perspective. The ranking is compiled with the use of each of the evaluation criteria on the basis of weighted average, taking account of the up-to-datedness of the input data of pre-defined materiality coefficients, and on the basis of arithmetic average representing the median value for the calculation of absolute volatility expressed by the standard deviation.
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