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Use of Mobile Device Sensors in a Car
Aron, Lukáš ; Široký, Vít (referee) ; Žák, Pavel (advisor)
This thesis analyses the use of an accelerometer commonly present in a mobile device for the measuring of vibration in a vehicle. Such measurements are used to map the quality of the road surface. The signal from the accelerometer and the current travelling speed of the vehicle are evaluated according to a threshold function. This function allows for a determination of whether the signal reported by the device signifies a pot-hole or another type of defect on the road surface. If the defect is determined to pose a threat to a vehicle travelling at higher speeds, then this fact is saved into the memory of the device. The second part of this thesis deals with the design of an internet and a mobile application, which is able to collect all measured data processes using cluster analysis and then shares the results with other participating devices. Tha practical results is that a map of road surface defect is thus created, which is then displayed to road users. This thesis deals with theory of mobile device sensors, including signal processing, as well as the design creation of the internet and mobile application.
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Influence of manufacturing process and precision on quality of liquid atomization from pressure-swirl atomizers
Lebeda, Kryštof ; Cejpek, Ondřej (referee) ; Malý, Milan (advisor)
The thesis deals with the effects of manufacturing inaccuracies and manufacturing method on the spray quality of pressure-swirl atomizers. First, basic principles of atomization are outlined. In the next section, observed atomization parameters of pressure swirl atomizers are discussed. This is followed by a research of available studies investigating influence of inaccuracies and manufacturing methods on selected parameters. In the practical part evaluation of measured data of pressure-swirl atomizers with defects at discharge orifice, different surface roughness and atomizers made by 3D printing was carried out. Skewness and misalignment of discharge orifice significantly reduced spray quality. No effect of surface roughness for conventionally manufactured nozzles was found. The use of 3D printing for nozzle production resulted in a deterioration of atomization fineness. Chamfering of dicharge orifice promoted larger spray cone angle but did not affect the atomization fineness.
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Smart Metering of Water Supply System at Campus of Faculty of Civil Engineering BUT
Černíková, Eva ; Ručka, Jan (referee) ; Tuhovčák, Ladislav (advisor)
The aim of this thesis is to perform an analysis of possibilities and benefits of installing the Smart Water Metering technology at campus of Faculty of Civil Engineering, Brno University of Technology. The main goal was to provide specific solutions of Smart Metering systems from different companies. One part of this thesis is dedicated to a detailed analysis of a water consumption measurement campaign that took place at the beginning of this academic year using dataloggers. Real-time flow rate and consumed volume was recorded every five minutes. Therefore it was possible to determine water consumption patterns during the day and also tell the minimum, maximum and average flow rate in different parts of the campus. Thanks to these measurements, irregular water consumption during the night was observed. This would not have been easily detected without recording real-time data. This system of recording real-time flow rates with dataloggers is considered to be suitable for the needs of faculty. Thanks to a GSM module, recorded values are sent to an FTP server once a day. From there the data can be downloaded to any kind of analysis software. Installation of Smart Water Metering technology at campus of Faculty of Civil Engineering would be beneficial for both operation of the water supply network and also for purposes of an academic research.
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The Study of Biopolymers Stability Using Light Scattering Techniques
Kratochvíl, Zdeněk ; Sedláček, Petr (referee) ; Kalina, Michal (advisor)
The stability of chosen biopolymers (or simple carbohydrates and amino acids) was in-vestigated using light scattering methods. Samples were prepared by dissolving the sub-stance in deionised water or in diluted acetic acid. Firstly, the effect of increasing concen-tration on zeta potential was observed and the optimal concentration for the next measure-ments was determined. Afterwards, the stability and the molecular weight of used sub-stances were studied during long-time storage in different time intervals. It was found out that chitosan, CMC, hyaluronate and sodium alginate were subjected to degradation whereas increase of the molecular weight and the zeta potential was observed in case of BSA. In the end, the samples were exposed to several different external effects, namely the influence of different temperatures, UV radiation and increasing ionic strength. The results showed that rather higher temperature has a significant impact on biopolymers stability. However, used biopolymers were hardly affected by UV radiation except BSA whose molecular weight increased considerably. Decrease of the zeta potential absolute values and the molecular size usually occurred with increasing ionic strength. The change of molecular weight was not detected.
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Production of polyhydroxyalkanoates employing Haloferax mediterranei
Hlináková, Kristýna ; Kučera, Dan (referee) ; Obruča, Stanislav (advisor)
This bachelor thesis is focused on study of selected effects on production of both polyhydroxyalkanoates (PHA) and extracellular polysaccharides (EPS) by halophile Archae Haloferax mediterranei. Theoretical part deals with a brief characterization of biopolymers and provides overwiev of the relevant microbial producers. The experimental part is focused on selection of a suitable substrate, concentration of sodium chloride and glucose in culture medium for a optimal production of biopolymers. Effects of light and suitable cultivation temperature were studied as well. UV-VIS spectrophotometry and gravimetry were used for analysis of biomas content. The content of PHA was analysed by gas chromatography with FID detection. PHA produced by H. mediterranei is a copolymer P(3HB-co-3HV), 3HV content is about 10%. The mixture of glucose and glycerol proved to be the most suitable substrate for a production of PHA, with a content od 80,6% of PHA. Further, we investigated optimal concetration of NaCl, which was found at 200 g/l. Interestingly, microbial culture responded to various salt concertations by differences in pigment formation. Finally, the last parameter optimalized in this work was temperature of cultivation. The highest content of PHA was obtained at 37°C, but the culture was capable of PHA accumulation and growing also at 50°C.
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Interactions and aggregation in hyaluronan-aminoacid-surfactant systems
Venerová, Tereza ; Velebný, Vladimír (referee) ; Pekař, Miloslav (advisor)
The effect of native hyaluronan addition on CMC of excluded surfactants (Tween 20, BETADET THC 2, SDS, CTAT a CTAB) in physiological solution (0,15 M NaCl) has been investigated by fluoresence spectroscopy with Pyrene and Nile red as probes. The greatest influence on CMC has been observed in systems with cationic surfactant. System of cetyltrimethylamonnium bromid has been closely studied. Solubilization experiments with a hydrophobic dye Sudan red has been realized and aggregation number of this system has been determinated via fluorescence quenching with cetylpyridinium chloride as quencher and Pyrene as fluorescence probe. Addition of hyaluronan (native or hydrophobicaly modified) reduces aggregation number of system.
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Development of the construction of residential properties in Brno Černá Pole
Kaletová, Lenka ; Urbánek, Zdeněk (referee) ; Klika, Pavel (advisor)
This Diploma thesis is dealing with development construction of residental real estates in the area of Brno – Černá Pole. It is concentrated on the development of real estate from the beginning of development in the sixties of 19th century till present. It is also include Černa Pole´s history. The work is divided into few construction periods, where is discribed in detail survey of the significant residental buildings. The highest quality development was during the First republic. Development of this area is almost finished in the seventies of 19th century and from this time it is mostly focused on building attics or reconstraction. The work contains maps, where we can see how was the area amplify.
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Magic Lantern Video Decoder for Canon 5D Camera
Škvařilová, Radka ; Musil, Petr (referee) ; Zemčík, Pavel (advisor)
Tato práce představuje návrh na vytvoření dekodéru pro video zaznamenané pomocí softwaru Magic Lantern, který může být nainstalován na Canon 5D. Toto video je význačné pro svoji velikost 14-bitů v raw formátu a proto může produkovat velmi kvalitní výstup. Práce má za cíl rozdělit video do jednotlivých snímků, ve vhodném formátu, který umí pracovat také s formáty obrazů s vysokým dynamickým rozsahem.
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Determining motion using accelerometer data
Montilla, Michaela ; Koťová, Markéta (referee) ; Janoušek, Oto (advisor)
The aim of this thesis is to present the topic of acceleration record using accelerometer sensors and to create a program for the transformation of the acceleration data into the position vector. It is a summary of theoretical background of data recording using sensors of acceleration and an overview of the physical relationships between acceleration, velocity and position. The data was recorded by multiple types of sensors. The gist of the algorithm is a mathematical realization of physical relations modifying the conversion of acceleration to the position. The outcome enables to display a dynamic graphic output in 3-D.
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Collagen cross-linking using oxidized cellulose
Filka, Pavel ; Márová, Ivana (referee) ; Vojtová, Lucy (advisor)
Předložená diplomová práce v teoretické části shrnuje základní fakta o kolagenu, bílkovině, která je nejrozšířenější v lidském organismu a oxidované celulóze používané v medicíně po několik desetiletí. Hlavním tématem této časti je síťování kolagenu, které je důležitým faktorem pro stabilizaci kolagenu podporující odolnost proti jeho degradaci. Jako síťující činidlo lze použít právě oxidovanou celulózu, která má kromě hemostatického účinku i funkční karboxylové skupiny vhodné k síťování proteinů. Praktická část práce byla zaměřena na sledování vzájemného chování směsí roztoků oxidované celulózy a kolagenu. Filmy či pěnové lyofilizáty připravené z těchto polymerních směsí by mohly sloužit jako účinná hemostatika nebo jako antibakteriální krytí ran podporující hojení. Byla zvolena řada hmotnostních poměrů mezi kolagenem a oxidovanou celulózou (9:1, 3:1, 5:3, 1:1, 1:2, 1:3, 1:9) se zachováním konstantního množství kolagenu, ale se vzrůstajícím množstvím celulózy. Jejich schopnost chemicky se vázat a umožnit tak vznik amidových vazeb mezi volnými aminovými skupinami kolagenu a karboxylovými skupinami oxidované celulózy byla sledována pomocí dvou UV-VIS spektroskopických metod, které využívají barevné reakce chemického činidla (ninhydrinu či 2,4,6 trinitrobenzensulfonové kyseliny) se zbylými volnými aminovými skupinami kolagenu. Karboxylové skupiny oxidované celulózy byly navíc aktivovány jak v polymerním roztoku tak i ve formě filmu směsí činidel 1-ethyl-3-(3-dimethylaminopropyl)karbodiimidu a N hydroxysukcinimidu (EDC/NHS). Pomocí infračervené spektroskopie s Fourierovou transformací (FT-IR) byly vyšetřeny změny vzorků na úrovni sekundární struktury kolagenu. Stabilita připravených směsí byla sledována ve formě filmu pomoci hydrolytické degradace při 37 °C. Morfologické změny na dvou typech lyofilizovaných vzorků, vymražených rychle při 196 °C nebo pomaleji při -30 °C, byly sledovány pomocí rastrovací elektronové mikroskopie (SEM). Při přípravě polymerních směsí se obě složky (kolagen i celulóza) se vzrůstajícím obsahem celulózy srážely až do poměru 1:1. UV-VIS analýzy potvrdily pokles volných –NH2 skupin poukazující na síťování kolagenu s celulózou shodně s nárůstem odolnosti vůči hydrolytické degradaci získané z měření úbytků hmotností připravených filmů. Od poměru 1:2 se složky již nesrážely, polymerní roztok byl homogenní, ale z důvodu nárůstu počtu volných aminových skupin od tohoto poměru výše lze usoudit, že celulóza fungovala v malém obsahu pouze jako fyzikální síťovalo a po dosažení rovnovážného stavu s kolagenem funguje spíše jako rozpouštědlo. Tímto způsobem zřejmě mohlo dojít ke změnám kolagenu až na úrovni sekundární struktury zaznamenané pomocí FT-IR. Aktivace karboxylových skupin celulózy činidly EDC/NHS nebyla prokázána. Poměry složek ovlivnily i porozitu a velikosti pórů připravených lyofilizátů určených pomocí SEM. Do poměru 1:1 byla porozita skafoldů vymražených kapalným dusíkem mezi 46 – 60 %, po dalším přidání celulózy stoupla až na 81 % (u poměru 1:9). Průměrná velikost pórů samotného kolagenu byla velice malá (14 ± 5 m), oproti oxidované celulóze (79 ± 24 m), proto přídavek celulózy vždy zvýšil velikost pórů na cca 55 m s výjimkou poměru 1:9, mající vysokou průměrnou velikost pórů (186 ± 76 m) a velmi pravidelnou strukturu připomínající včelí plást, kterou má i samotná celulóza.
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