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Herbal alternative treatment for various diseases
MICHALÍK, Petr
The theme of the thesis is Herbal alternative treatment for various diseases. Bachelor thesis is focused on medicinal plants used by the human population since time immemorial, which are used to this day, not only for treatment, but also for prevention. The introduction mentioned general part, then the history of herbal medicine, construction plant, positive and negative effects of the herbs, contained substances of herbs, harvesting, drying and finally mentioned individual herbs. The aim of this study was to map the use of herbal alternative treatments to the public within the primary and secondary prevention. The basis for the continuing part happened questionnaire regarding age population from 18 years, the results of which revealed some interesting information about the public awareness of this method of treatment using medicinal plants. The results also showed a preference for herbal or conventional treatment, then what are the most practiced methods of natural medicine and the most popular form of their use. The results also show that respondents still consider herbal therapy as an important complement to conventional medicine. Based on these findings, conclusions were drawn and set out recommendations for practice.
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In vitro cultures of medicinal plants - XIII.
Kubeš, Jan ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Jan Kubeš Genista tinctoria in vitro - abiotická elicitace Cultures of medicinal plants in vitro - XIII. The plants cultures in vitro contain lesser amounts of secondary metabolites in compare with intact plants. The elicitors can affect these metabolites production. The effect of electric current (50mA) of different voltage (5, 7, 8, 10, 15, 20 and 24 V) and different time exposition (10, 30, and 60 minutes) on content of isoflavones was studied on (Genista tinctoria) suspension culture. The highest concentration of genistin (0.17 %) was measured in suspension culture after 30 min of elicitation by 10 V after 6 hours of cultivation. The highest concentration of daidzein (0.35 %) was found in suspension culture after 60 min of elicitation by 5 V after 24 hours of cultivation. The highest concentration of genistin (1.6 mg/100ml) was measured in medium after 30 min of elicitation by 5 V after 24 hours of cultivation. The highest concentration of daidzein (1.77 mg/100ml) was found in medium after 10 min of elicitation by 24 V after 6 hours of cultivation.
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In vitro cultures of medicinal plants - XII.
Janoutová, Martina ; Tůmová, Lenka (advisor) ; Dušková, Jiřina (referee)
Martina Janoutová In vitro cultures of medicinal plants - XII. Abstract The effect of ultrasound (US) as abiotic elicitor on the flavonolignans production in Silybum marianum L suspension culture was investigated. The culture was cultivated in Murashige and Skoog nutritive medium with ( - NAO) (g/l) as growth regulator at 25o C and luminous period 16 h of light and 8 h of darkness. The elicitor - ultrasound by frequency 35kHz and intensity 0,1Wcm-3 for a period 1, 2, 3, 4 and 5 min has been used. The samples were taken in 0, 6, 12, 24, 48, 72 and 168 h after US exposition. The control samples were taken in 0 and 48 h. The quantity of flavonolignans was determined by High performance liquid chromatography (HPLC). The highest increase of taxifolin content was apparent after 5 min of US elicitation and 48 h sampling (0,04%) - 400%, other increase was apparent after 5 min of elicitation and 72 h sampling. The higher content of silychristin was found after 1 min of US elicitation and 72 h sampling, the same level was observed after 2 min of elicitation and 24 h sampling. The higher level of silydianin was detected after 2 min of US elicitation and 6 h sampling and the silybin B after 2 min of elicitation and 12 h sampling after exposition. Taxifolin and flavonolignans release to the nutrient medium was...
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In vitro cultures of medicinal plants - XI.
Sojková, Kristýna ; Tůmová, Lenka (advisor) ; Kašparová, Marie (referee)
In vitro cultures of medicinal plants - XI. Elicitation is one of the few strategies that can be used in enhancement of secondary metabolites production from explant cultures. The effect of abiotic elicitor (silver nitrate) on flavonolignan and flavonoid taxifolin production in suspension culture of Silybum marianum L. (Gaertn.) and on isoflavones production in suspension culture of Genista tinctoria L. was tested. Silver nitrate in various concentrations (5.887.10-3 mol/l; 5.887.10-4 mol/l; 5.887.10-5 mol/l) was used as elicitor. Content of secondary metabolites in suspension cultures was determinated by high performance liquid chromatography (HPLC). The samples were taken after 6, 12, 24, 48, 72 and 168 hours after elicitor treatment. The highest content of taxifolin production (0.02 %) in suspension culture of Silybum marianum L. (Gaertn.) after silver nitrate (5.887.10-4 mol/l) treatment and 72 h sampling was detected. The highest content of genistin (0.05 %) in suspension culture of Genista tinctoria L. was found after silver nitrate (5.887.10-4 mol/l) treatment and 48 h sampling. The highest content of daidzein (0.09 %) was detected after elicitor (5.887.10-4 mol/l) treatment and 168 h sampling.
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NADP-dehydrogenases of medicinal plants.
Bartošová, Martina ; Hýsková, Veronika (advisor) ; Kubíčková, Božena (referee)
Medicinal plants usually contain metabolites formed by secondary biosynthetic pathways for example phenylpropanoid and shikimate pathway, which depend on NAPDH availability. In this work total content of phenolic compounds and flavonoids was determined in 10 medicinal plants common for Czech Republic. Activity of enzymes providing NADPH was determined as well. These enzymes were glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), NADP-isocitrate dehydrogenase (NADP-ICDH, EC 1.1.1.42), NADP-malic enzyme (oxalacetate decarboxylating) (NADP-ME, EC 1.1.1.40). The activity of shikimate dehydrogenase (SDH, EC 1.1.1.25), the main enzyme of shikimate pathway, was also determined. Extraction methods were optimized for each assessment. Applicable source for NADP-dependent enzymes are black radish (Raphanus sativus L. var. niger), garlic (Allium sativum L.) and mint (Mentha piperita L). The highest content of phenolic compounds and flavonoids was determined in sage (Salvia officinalis), lemon balm (Melissa officinalis L.) and Mentha citrata. Extraction with methanol was more effective than extraction with hot water. More extraction methods and studies are necessary for characterization of individual compounds and principals on which they work. Key words: medicinal plants, NADP-dependent enzymes, phenolic...
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Healing plants from labiate family (Lamiaceae) and their usage in cross subjects relationship between biology and chemistry
Musilová, Hana ; Skýbová, Jana (advisor) ; Pavlasová, Lenka (referee)
Healing plants from labiate family (Lamiaceae) and their usage in cross subjects relationship between biology and chemistry. The presented diploma work deals with healing plants. It is mainly focused on cross subjects of chemistry and the usage of theory in practical life. Theoretical part of this work consists of an overview of selected races. It's aim is mainly to present substance content of these plants, specific effects and wide usage. The usage of important characteristic features of this family in teaching and proposals how to classify this topic as a learning subject at primary schools and grammar schools is the further content of this work. Practical part is a result of questionnaire research, which should give us some information about how teachers at secondary schools work with this topic in practical lessons. The final outcome of practical part are six practical activities that teachers can use while teaching, mainly at high schools. Further learning material contains four presentations in PowerPoint and six worksheets which relate to laboratory and practical exercises. Final outcome of the work is information about this family set to a compact text and further visions for didactic use in teaching.
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Aroma active compounds of selected types of medical herbs
Pecinová, Ester ; Diviš, Pavel (referee) ; Vítová, Eva (advisor)
This thesis deals with aroma active compounds of selected types of medicinal herbs, specifically lavender (Lavandula officinalis), peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and hyssop (Hyssopus officinalis). In the theoretical part the single herbs were characterized including botanical description, chemical composition, medicinal properties, adverse effects and the practical use; aroma active compounds present in herbs are also emphasized. Based on the literature search, gas chromatography coupled with mass spectrometry as currently the most frequently used method, was chosen for the assessment of aroma compounds. Solid Phase Microextraction was used for isolation of volatile compounds from dried herbs. Within experimental part of the work selected parameters of the SPME-GC-MS method were optimized, aroma compounds in samples of herbs were identified, and twenty four suspected fragrance allergens due to the Regulation (EC) N° 1223/2009 were also identified and quantified. Overall 69 compounds were indetified together with 2 allergens in lavender, 59 compounds including 5 allergens in peppermint, sage contained 49 compounds with 4 allergens included, 41 compounds were identified in St. John’s wort including 4 allergens and finally 64 compounds with 4 allergens included in hyssop.
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Laboratory evaluation of activity of traditional Ecuadorian medicinal plants
Klepáčková, Kateřina ; Klouček, Pavel (advisor) ; Přemysl, Přemysl (referee)
Health care in Ecuador varies greatly depending on socioeconomic status, age, sex and place of residence. One third of the population has no regular access to health services and more than two-thirds of the population has no health insurance. Herbal medicine is
the synthesis of therapeutic experience of indigenous physicians which has been evolving
for hundreds of years and its resources are used to prevent and treat various types of diseases. Plants are popular natural remedy due to their efficacy, safety and minimal side effects. However, certain combinations of herbal medicine and drugs can be dangerous. Sciences, which help to examine the context of the traditional use of plants in the timeframe and also contribute to the discovery of their bioactive compounds, are called ethnobotany and ethnopharmacology. This paper investigates ten plants imported from Ecuador, which are traditionally used for healing various types of diseases such as gastrointestinal disorders, influenza, cold, urinary tract infection, skin diseases in the pelvic cavity etc. Efficiency research of herbal medicine has a great significance for future healthcare because
of the expansion of antimicrobial resistance, which is currently becoming a serious health risk for both humans and animals. Antimicrobial and antioxidant activity of plants, which are
in this thesis measured by the broth microdilution method and ORAC method, are important indicators for the analysis of medicinal plants. The only demonstrable positive result
of testing the antimicrobial activity was the discovery of minimum inhibitory concentration
of Espeletia schultzii against Streptococcus pyogenes (MIC=0.256 mg/mL). The highest antioxidant activity was discovered in the plant Croton elegans Kunth (743,64 +- 28,56 microgram of trolox equal to 1 miligram of the extract), for which no records associated to antioxidant activity were found.
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Medical Plants in the Contenporary Family Graden
Vohralíková, Sabina ; Buttry, Ivana (advisor) ; Hladíková, Lucie (referee)
The first part of Bachelor thesis is focused on historical evolution of using medicinal plants in each period of time. Emphasis is mainly put on significant herbal doctors and their important pieces, influencing herbals utilizing since ancient times until now. In this work certain plants are presented for healing effects, used in specific historical times. A Separate chapter is devoted to the medieval and monastery gardens in terms of growing medicinal plants especially in Apothecary garden.
The main part covers the selection of plants used for medicinal effects in folk medicine. Plants are described from botanical point of view, including placing requirements on habitat. Furthermore the separate healing parts of plants used in in folk medicine are stated. Each herb is mentioned with respect to its possible application in aesthetic garden. Herbs are chosen in specific way in order to fulfill habitat conditions for their growing along with other plants in a flowerbed.
The third part includes distribution of selected plants into groups, according to height growth, habitat conditions, flower color and possibility of using in the garden composition. The output is a description and sketch of several model plantations, which are compiled only from medicinal plants.
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Plants traditionally used in the Czech Republic for their antimicrobial properties: An overview and comparison with scientific studies
Ježková, Pavlína ; Nový, Pavel (advisor) ; Houdková, Markéta (referee)
The use of plants in medicine and folk medicine is probably as old as humanity itself. Medicinal plants have accompanied human throughout the stages of its historical development and herbs were the first medicines which human met. Healing herbs were the most accessible and often only therapeutic agent.
In the first part of the thesis, the information about the history of medicinal plants, natural method of therapy, phytotherapy, biologically active substances of plant and about antimicrobial agents are summarized. Some specific cases of plants with antimicrobial activity are also listed.
The second part presents data obtained by comparing data on the traditional use of plants for their antimicrobial activity in folk medicine to scientific data available from the Web of Science database. The traditional use of plants was investigated according to data from the most comprehensive Czech herbarium of medicinal plants from Jiří Janča and Josef Antonín Zentrich. From a total of 363 plants, 207 species whith presumable antimicrobial activity were selected from the herbarium,. For these plants were subsequently searched and evaluated all records from scientific databases mentioning their antimicrobial activity.Sbsequently, all records on these species mentioning their antimicrobial activity were searched on the scientific database and evaluated.
After the comparison and evaluation of the data gathered about the initial 207 species, 31 plants, which should exhibit antimicrobial effects according to the aforementioned herbals but scientific records are confirmed unsatisfactorily or not at all, were selected. This group of plants can serve as the basis for future research to confirm or refute their antimicrobial activity.
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