National Repository of Grey Literature 60 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1
Šachová, Ivana ; Martin, Jan (advisor) ; Mladěnka, Přemysl (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Ivana Šachová Supervisors: PharmDr. Jan Martin, PhD., Dipl. Ing. Mgr. Ferdinand Molnár, PhD. Title of diploma thesis: Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1 The aim of this study was to express wild type and mutated synthetic human SRC1 central domains in E. coli BL21 DE3 cells. Expressed recombinant proteins were purified afterwards and used for investigation of SRC1-CAR protein interaction using pull down experiments. The constitutive androstane receptor (CAR), a nuclear receptor (NR), is a transcriptional regulator, which influences the expression of various proteins, such as enzymes of biotransformation and transporters important for metabolism of both endogenous and exogenous compounds. CAR can be fully active only in presence of coactivators, such as steroid receptor coactivator-1 (SRC1). The most important fragment of SRC1 for binding to NRs is the central domain, made of three α-helical Leu-X-X-Leu-Leu (LXXLL) L1, L2, L3 motifs, in where L is a leucine and X represents any amino acid. hSRC1, as well as hCAR, can be successfully produced by expression of cDNA of interest in E. coli strains. In case of SRC1,...
Natural drugs in the treatment and prevention of prostate diseases
Alaei Faradonbeh, Danial ; Martin, Jan (advisor) ; Tůmová, Lenka (referee)
Danial Alaei Faradonbeh, Natural drugs in the treatment and prevention of prostate diseases, Charles University in Prague, faculty of pharmacy in Hradec Králové, 2015, 86 pages. "Natural drugs in the treatment and prevention of prostate diseases" deals with prostate diseases which are some of the common ailments affecting men in different parts of the world. The etiology of prostate diseases has been identified but little progress has been witnessed as far as treatment is concerned. Of all prostate diseases identified so far, prostate cancer is the most common and affect me aged 40 and above. Prostate cancer affects the prostate gland and affects the normal function of other genitourinary tissues. Conventional prostate disease therapies have yielded minimal results, leading to increased calls for further research. Successful application of plants in the management of other conditions has attracted the interests of cancer researchers. Focus on a number of plants can provide the much needed reprieve and therapy against prostate cancer. Previous studies have identified a number of plants whose active component can act against cancer cells. Ficus pseudopalma, Nelumbo nuficera, Camptotheca acuminata, Rauvolfa vomitoria and Viscum album are some of the plants which have been identified to act against...
Ellagitannins -occurence, metabolism and effects on human body
Raabová, Karin ; Karlíčková, Jana (advisor) ; Martin, Jan (referee)
CHARLES UNIVERSITY PHARMACEUTICAL FACULTY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY AND ECOLOGY Title of the Diploma thesis: ELLAGITANNINS - OCCURENCE, METABOLISM AND EFFECTS ON HUMAN BODY Candidate: Bc. Karin Raabová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Diploma thesis 2016/2017, pp. 77 Ellagitannins belongs to a class of hydrolysable tannins, which are susceptible to hydrolysis to give ellagic acid in the digestive tract. Ellagitannins occur in many plant families, for example plants in the family Rosaceae, Myrtaceae or Lythraceae. There are naturally found in some fruits (pomegranate, strawberries, blackberries, raspberries, grapes), but also in the seeds of walnuts and thus form a diverse group of bioactive polyphenols with anti-inflammatory, antitumor, antioxidant and antimicrobial activity. Special instrumental methods (HPLC, DAD, MS) are most often used for subsequent evidence of occurrence of these compounds in plants and their identification. This diploma thesis is a literature review, which aimed at processing the available knowledge about the ellagitannins. Attention was focused on the biological activity demonstrated in an animal or human organism. Keywords: ellagitannins, occurrence, metabolism, effects, human body
Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1
Šachová, Ivana ; Martin, Jan (advisor) ; Mladěnka, Přemysl (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Ivana Šachová Supervisors: PharmDr. Jan Martin, PhD., Dipl. Ing. Mgr. Ferdinand Molnár, PhD. Title of diploma thesis: Preparation of strategy for studying interaction of human constitutive androstane receptor with steroid receptor coactivator-1 The aim of this study was to express wild type and mutated synthetic human SRC1 central domains in E. coli BL21 DE3 cells. Expressed recombinant proteins were purified afterwards and used for investigation of SRC1-CAR protein interaction using pull down experiments. The constitutive androstane receptor (CAR), a nuclear receptor (NR), is a transcriptional regulator, which influences the expression of various proteins, such as enzymes of biotransformation and transporters important for metabolism of both endogenous and exogenous compounds. CAR can be fully active only in presence of coactivators, such as steroid receptor coactivator-1 (SRC1). The most important fragment of SRC1 for binding to NRs is the central domain, made of three α-helical Leu-X-X-Leu-Leu (LXXLL) L1, L2, L3 motifs, in where L is a leucine and X represents any amino acid. hSRC1, as well as hCAR, can be successfully produced by expression of cDNA of interest in E. coli strains. In case of SRC1,...
The antioxidant activity of St. John's wort explantate cultures
Vrabec, Rudolf ; Martin, Jan (advisor) ; Spilková, Jiřina (referee)
St. John's wort is a herbal medicine well known for its anti-depressant effect. It contains anthraquinone derivates, prenylated floroglucinols, tannins, hydroxycinnamic acids, and especially flavonoids, which have a wide range of biological effects on the body including antioxidant properties. Antioxidants are one of the possibilities to protect body from harmful influences of free radicals - highly reactive particles that damage biological structures. In this work, two methods (DPPH and superoxide radical) were used to determinate antioxidant activity of methanolic and aqueous extracts of dried St. John's wort - Hypericum Perforatum L. and its callus and suspension explant cultures. The results are processed into tables and graphs which compare the effectiveness of extracts to quench the radicals. EC50 for the methanolic extract was 0,064 mg/ml and for the aqueous extract 0,068 mg/ml. EC50 for the aqueous extract was 16,727 mg/ml, but unfortunatelly the other antioxidant activity values for EC50 were too low to evaluate. Determination of the content of flavonoids hyperoside and quercetin by high performance liquid chromatography shows that their highest content is in the drug extracts and that the explant cultures have negligible content of the flavonoids. There is no content of hypericin in all...
The selenium effect on secondary metabolites production in in vitro cultures of medicinal plants - I
Černá, Pavlína ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Higher plants represent an important source of valuable substances, so called secondary metabolites, which can be obtained through explant cultures of plants. Elicitation is a method of increasing the secondary metabolites production. This study aims to evaluate the secondary metabolites production in Fagopyrum esculentum variety Spacinska cultures in vitro after abiotic elicitor treatment. The experiment was focused on alteration of rutin production in callus and suspension cultures of F. esculentum var. Spacinska after selenium application. Murashige and Skoog nutrient medium supplemented with 1 mg l-1 2,4-dichlorophenoxyacetic acid was used for the cultivation. Selenium solutions of various concentrations (c1 = 9.012×10-3 mol l-1 , c2 = 9.012×10-4 mol l-1 , c3 = 9.012×10-5 mol l-1 ) were affecting the cultures for 6, 12, 24, 48, 72 and 168 hours. The content of rutin was determined by HPLC. The release of secondary metabolites into the nutrient medium was studied as well. After elicitor application, the rutin production increased in both callus and suspension cultures. Higher levels of rutin content were detected in callus culture. The maximum rutin content (0.6 mg g-1 DW) was reached after 12 h of elicitor treatment of c2 concentration in callus culture. Concerning suspension culture, the...
Flavonoids in some cultivars of Sambucus nigra L. flowers.
Krulišová, Markéta ; Spilková, Jiřina (advisor) ; Martin, Jan (referee)
The aim of this work was to find out the content of flavonoids in the flowers of eleven cultivars of black elder (Sambucus nigra L.) and determine if their contents differ according to the statistical significance. Preparing the list of effects and substances that are contained in the flowers was the goal as well. The drug Sambuci nigrae flos is mostly used for its content of flavonoids and hydroxycinnamic acids in the therapy of colds and diseases of the urinary and respiratory tract. Its benefits were confirmed also by studies testing antibacterial and antiviral activity, diabetes and obesity, effects on the immune system and also protection against UV radiance. The content of flavonoids was established by the spectrophotometric method that is listed in the Czech Pharmacopoeia 2009 in the article Sambuci nigrae flos as the method for the determination of content. The statistic evaluation of the differences in the flavonoids content between the cultivars was made by ANOVA and the Bonferroni test. The demand of Czech Pharmacopoeia 2009 on the minimal content of flavonoids is 0,80%. This requirement was fulfilled in the flowers of these cultivars: Albida, Heidegg 13, Riese auß Voßloch, Sambu, Samdal, Sampo and Samyl. On the opposite side, flowers of the cultivars Allesö, Aurea, Dana and Juicy did...
In vitro cultures of medicinal plants IXX
Seidlová, Markéta ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
In vitro cultures of medicinal plants - IXX The subject of this thesis is the evaluation of secondary metabolites production in in vitro cultures of Silybum marianum L. after elicitor treatment. In this study selenium dioxide as elicitor in concentrations of 9,012.10-3 mol/l; 9,012.10-4 mol/l; 9,012.10-5 mol/l was used. The samples were taken after 6, 12, 24, 48, 72 and 168 hours of elicitor treatment. The effect of elicitor was compared with control samples, which were cultured without elicitation. The content of taxifolin and flavonolignans was determined by the method of HPLC. The results showed, that almost all observed metabolites were released into a nutrient medium. Cells of callus and suspension cultures produced only small amounts of taxifolin (0.01 mg/g DW). Taxifolin and silymarin complex releasing into nutrient media was observed as in control and also in the elicitated samples of callus and suspension cultures. Selenium dioxide elicitation caused statistically significant increases in releasing taxifolin and silymarin complex into the nutrient medium. The statistically significant releasing of flavonolignans (2.2 mg/100 ml) to the medium of suspension culture was reached after 72 hours of treatment with selenium dioxide in concentration of c1 (9,012.10-3 mol/l). The statistically...
Production of secondary metabolites in the explant cultures of Trichocereus pachanoi (Cactaceae)
Urbanová, Lucie ; Martin, Jan (advisor) ; Tůmová, Lenka (referee)
Production of secondary metabolites in the explant cultures of Trichocereus pachanoi (Cactaceae) Lucie Urbanová Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Pharmacy Key words: Trichocereus pachanoi, the explant cultures, mescaline, secondary metabolites, prodrugs, elicitation, cinnamic acid, sodium cinnamate, tryptophan, ozone, methylene blue The aim of this thesis was the research of effect of selected precursors and elicitors for secondary metabolite production of mescaline in explant cultures Trichocereus pachanoi. Suspension cultures were cultivated on the Murashige and Skoog medium with addition of the growth regulators 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. To observe changes in production these precursors: cinnamic acid, sodium cinnamate and tryptophan, and these elicitors: ozone and methylene blue, were selected. Precursors were prepared and used in concentrations of 50, 100 and 500 mg·l-1. Ozone was added in two time slots, 5 and 10 seconds. Methylene blue has been used in concentrations of 1, 10 and 100 mg·l-1. Cultivation with added compounds lasted for 24 and 168 hours. The content of mescaline was determined by HPLC analysis. A statistically significant effect on the production of mescaline in vitro culture was proved only with...
Antioxidant properties of Sambucus nigra flowers. II
Kozoňová, Pavlína ; Spilková, Jiřina (advisor) ; Martin, Jan (referee)
Drug Sambuci nigrae flos - Sambuci nigrae flos is obtained from the wild plants. The drug is primarily used to help reduce the symptoms of colds and flu. Elder flower provides antioxidant, anti-inflammatory, anticancer and diuretic effects. The main substances are flavonoids and hydroxycinnamic acid. Elderflowers from bred cultivars are not used to obtain drug so far. The aim of this work was to determine antioxidant acitivitiy of extracts of eldeflower cultivars. Cultivars ′Albida′, ′Allesö′, ′Aurea′, ′Bohatka′, ′Dana′, ′Haschberg′, ′Heidegg 13′, ′Juicy′, ′Korsør′, ′Mammut′, ′Pregarten′, ′Riese aus Voßloch′, ′Sambo′, ′Sambu′, ′Samdal′, ′Sampo′, ′Samyl′, ′Tulbing′ and ′Weinhenstephan′ were analyzed. Antioxidant activity was measured spectrophotometrically using DPPH ((2,2-diphenyl-1-picrylhydrazyl) radical method. Results of antioxidant activity were expressed as IC50 (concentration required for 50% reduction of the DPPH radical). The measured values are in the range 0.0419 - 0.0772 mg/ml. Cultivars 'Heidegg 13', 'Albida', 'Tulbing' showed the highest antioxidant activity, whereas the lowest activity was found in 'Pregarten','Korsør' and 'Allesö' clutivars.

National Repository of Grey Literature : 60 records found   1 - 10nextend  jump to record:
See also: similar author names
1 Martin, Jakub
Interested in being notified about new results for this query?
Subscribe to the RSS feed.