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Cryopreservation of sensitive potato genotypes
Faltus, Miloš ; Bilavčík, Alois ; Zámečník, Jiří
This methodology describes the preparation of explants prior to cryopreservation, dehydration of shoot tips, procedure and principles of sample cryopreservation, regeneration of control plants, including evaluation of explant survival and regeneration for sensitive potato genotypes which were formerly very difficult to cryopreserve. Ministry of agriculture of the Czech Republic is the user of this methodology and it applies it in the framework of the "National program for conservation and utilization of plant genetic resources, animals and micro-organisms important for nutrition and agriculture".
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Biochemistry of polyphenols effects in the treatment of vascular disease
Kondrashov, Alexey ; Štípek, Stanislav (advisor) ; Pecháňová, Olga (referee) ; Matouš-Malbohan, Ivan (referee)
This Ph.D. thesis deals with the deep analysis of polyphenols effects toward vascular disorders. This work provides a number of experimental results of studying both the effects of natural bioactive compounds in red wines and potatoes, and their application to the experiments which includes experimental animal models with spontaneous hypertension. Methods and equipment used in experimental studies allowed us to make several new statements regarding the universal nature of the relationships between the antioxidant capacity and the polyphenolic content in examined foodstuffs. Besides this it is also recognized that food is a source of minerals which also contribute to the total antioxidant capacity and therefore may have influence the endogenous antioxidant enzyme system by providing the essential cofactors. Experimentally we have found that the concentrations of magnesium known for its therapeutic action in wine and red wine extract are comparable to the mineral waters recommended for the treatment and prevention of cardiovascular and metabolic diseases. Moreover, synergistic interactions between selected minerals have been found. The key issue of bioavailability of polyphenols for supporting the idea of the beneficial effects of diet rich in fruits and vegetables toward vascular disease prevention...
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Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains.
In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05).
In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter).
The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined.
Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb).
Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.
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Technology in potato cultivation aimed at efficient use of nitrogen
Svobodová, Andrea ; Hamouz, Karel (advisor) ; Miroslav, Miroslav (referee)
Environmental-friendly technology of potato growing was studied in field trial plots on Valečov Research Station belonging to Potato Research Institute Havlíčkův Brod between 2010 and 2014. The first part was focused on the effect of local application of mineral fertilizers on production yield and quality. The second part studied the effect of nitrogen fertilizer containing urease inhibitor (UREAstabil) compared to urea in increasing rates on yield and selected parameters of potato quality. In the first part of trials with liquid DAM 390, ammonium sulphate and urea the obtained results show that yield differences among selected fertilization variants were insignificant with certain tendency in favour of the variant with split nitrogen rate using DAM 390. For evaluated quality parameters no statistically significant differences among tested fertilization variants were recorded. In the second part of trials evaluating UREAstabil and urea applied at various rates during soil preparation prior to planting, applied rate had a higher effect on potato yields than nitrogen form. The results show that mutual comparison of urea and UREAstabil did not indicate any statistically significant yield differences.
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Realisation the Potato Market
Škába, Marek ; Kovářová, Kateřina (advisor) ; Škarková, Ludmila (referee)
This bachelor thesis deals with issues of the impact of quality on encashment of potatoes and their realization on the market. In first part of thesis are potatoes reviewed from morphological and physiological features and their chemical composition. Second part of thesis dealt with quality requirments of potatoes, internal and external quality of potatoes. Third part of thesis assesses the situation on potatoe market in the Czech republic and its trade possibilities.Last part is focused on price of potatoes and some factors that affects the price.
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The effect of water stress on the content of monosaccharides in potato tubers
Podhorecká, Klára ; Orsák, Matyáš (advisor) ; Hnilička, František (referee)
Bachelor thesis is focused on monitoring of influence on selected metabolites, especially monosaccharides. The first part is devoted to short characterization of potatoes, therefore the importance of this crop, morphology, cultivation and also are described demands of potatoes on environment and nutrition.
In the next section is detailed discussed problems of the chemical composition of tubers with a focusing on carbohydrates. Potatoes are globally consumed and are popular source of carbohydrates. Potatoes are rich in vitamins also, especially in vitamin C, B3 and B6. Potatoes contain a high content of minerals, especially potassium and magnesium, too. As another substances are in potatoes colorants, antioxidants, phenolic and related substances, proteins, and small amount of lipids. It is noted that proteins of the potato tuber are ranked among nutritionally valuable proteins of vegetable origin. Among this proteins is ranked Patatin, which is considered to be a major reserve protein and it is placed in the vacuoles. Substances, which has a negative effect on the human body are called Glycoalkaloids the opposite. Glykoalkaloids are synthesized by plants of the family Solanaceae. The most significant substances in potatoes are alfa-solanine and alfa-chaconine. Value of tubers is primarily determined by their chemical composition, which forms food and raw materials. The contents of sugars in the tubers are relatively low (0,5 % of the original mass), and even though their contents are important both in aspect of manufacturing and in sensory aspect. The main reducing monosaccharides are ranked glucose and fructose, typically with a concentration of 0,15 - 1,5 %. The sugar content significantly affects internal and external quality characteristics especially fried products. Several studies confirm that the accumulation of carbohydrates might help resist extreme stress conditions, especially low temperatures, salinity and water deficiency.
In the next chapter is created summary of stress conditions effects on plants with focusing on potatoes. In plants we can talk about stress, if variability of negative environmental factors exceeds a certain limit. The main theme and also the most limiting factor is deficiency of water in other words water stress. Also were mentioned other factors abiotic and biotic factors marginally.
By effects of stress reaction is created a specific level of adaptive skills. Temporarily it can lead to acclimatization, which increasing the resistance to abiotic stressors. Most of plants are trying to establish the tolerance to stress. In this Chapter were also described changes during storage and during heat treatments of potato, which leads to an undesirable darkening of the tubers. Excessive levels of reducing sugars cause unacceptable browning of fried products.
In the last chapter is described selected methods of determining carbohydrates. These methods are based on reducing properties of sugars, on their condensing reactions by color, measurement of optical activity, measurement of refractive index, separation methods and others.
The methodic of work clarifies the determination of reducing monosaccharides (glucose, fructose), sucrose by the high performance liquid chromatography (HPLC) in four selected varieties of potatoes from harvest of 2015.
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Study of diversity of weed communities in wide row crops in Podkrkonoší Region
Kocour, David ; Kolářová, Michaela (advisor) ; Václav, Václav (referee)
The main aim of the master's thesis was an assessment of weed communities in Podkrkonosi Region (the area underneath the Giant Mountains) (Pokrkonosi). The effect of the crop (maize, potatoes) and altitude with a particular focus on thermophilic weed specieshas been investigated.
The theoretical part outlines weed species referring to their significance, classification, harmfulness as well as benefits. The effect of climate changes, invasive plants, biodiversity and effect of temperature are discussed. Last part of the literary review is a brief description of model crops.
In the practical part of the thesis, soil and climate conditions of the locality are described. Furthermore, a term phytocoenology is introduced and the methodology of recording relevés and the data conversion are explained. Totally, 99 weed species out of 25 families were found (69 in maize, 85 in potatoes).
Chenopodium album agg., Echinochloa crus-galli and Fallopia convolvulus were the most frequent species in maize. The species with the highest cover were Fallopia convolvulus, Chenopodium album agg. and Echinochloa crus-galli.
Chenopodium album agg., Echinochloa crus-galli and Viola arvensis were the most frequent in potatoes. Chenopodium album agg., Fallopia convolvulus, Echinochloa crus-galli and Galinsoga quadriradiata can be classified as the weeds with the highest cover.
Euphorbia helioscopia and Sonchus arvensis were positively correlated with increasing altitude, while Amaranthus retroflexus, Solanum nigrum and Galinsoga quadriradiata were negatively correlated. Thlaspi arvense and Echinochloa crus-galli were characteristic species in maize, and Veronica persica, Galinsoga quadriradiata and Sonchus arvensis in potatoes.
It can be concluded that potatoes fields showed higher species richness than maize, especially in higher altitudes. Maize was characterized by narrow weed spectrum with few predominating species. The tested hypothesis was confirmed and a statistically significant effect of altitude on the occurrence of thermophilic weed species has been found.
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Differences in potatoes yield structure cultivated in organic and integrated growing technology
Erbenová, Veronika ; Hamouz, Karel (advisor) ; Milan, Milan (referee)
The aim of this study is to evaluate the impact of organic growing in comparison with conventional (integrated) cultivation on yield and its structure based on the results of its own field trials.
On a selected topic I have compiled a literature review based on the professional literature. To this theme I established a field experiment in Dolní Brusnice in 2015. I used two varieties of potatoes (Adéla and Marabel). The experiment was established in the two versions of the integrated and the organic farming, each in four repetitions with twelve consecutive clusters.
The results of the experiment in statistical evaluation showed that integrated cultivation significantly increases the yield of tubers under a bunch in comparison with the organic way of cultivation (in this case by 23 %). In the integrated variant was achieved higher average number of tubers under a clump versus the environmental variation. But it was only a trend. The result was inconclusive. The average weight of a tuber showed up again in an attempt to trend lower its value in organic farming but only for the Marabel variety. It was also assessed the damage mold leaf area in a potato, which was greater in the organic cultivation.
The results of this study can be applied when deciding on the establishment of conventional (integrated) or organic crop of potatoes for the production of marketable tubers. At the same time I would like to emphasize that the results are only one year and cannot be generalized. They can be built upon in subsequent experiments.
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Chemical weed management in potatoes
Smolík, Ondřej ; Hamouz, Pavel (advisor) ; Holec, Josef (referee)
Presented thesis is focused on the topic of regulation of weeds in potatoes. The advantages and disadvantages of chemical control of weeds are investigated, including the comparison of the results obtained in both herbicidal and mechanical treatments. The core of this thesis is to find out what kind of influence has weeds on potato yield and how is the weed infestation affected be herbicides. The research was undertaken by a field experiment with eight treatments. The first one brought a field completely without any interventions. On the second field a mix of Sencor Liquid (PRE), Command 36 CS (PRE) and Roundup Klasik (PRE) was used. The third treatment was sprayed by Sencor Liquid (PRE), Command 36 CS (PRE) and Pantera QT (POST). The fourth one was treated by both Sencor Liquid (POST) and Titus 25 WG (POST). The fifth treatment obtained only mechanical interventions (machine hoeing). On the sixth one Sencor Liquid (PRE), Command 36 CS (PRE) and Roundup KLASIK (PRE) were applied in combination with the mechanical treatmant. The seventh treatment used Sencor Liquid (PRE), Command 36 CS (PRE) and Pantera QT (POST), again combined with the mechanical treatment. On the last of the experimental fields Sencor Liquid (POST), Titus 25 WG (POST) and a mechanical treatment. Treatments 2-8 were compared to the first one. In this comparison it turned out that the herbicides help on a great scale with the regulation of the weed. By the treatments 2, 3, 6 and 7 the potato yield was three time higher compared to untreated control. Treatments 4 and 8 showed that the application of some post-emergent herbicides can (due to their phytotoxicity) decrease the yield to the level of the untreated plots.
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