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The Image of Mother in the Work of Tomáš H.
HRUBÁ, Helena
This bachelor´s thesis concentrates on relation of mother and child in its early childhood with consequent impacts of this relation on adulthood. It is set upon analogies between unsatisfactory mother-son relation in latter´s childhood and latent meaning of found symbolic of oral frustration or separation anxiety in son´s (who´s a professional artist) creative production. Concept of mother is mentioned here in all of its available symbolic modifications. Theoretic part concentrates on mother-child relation from psychoanalytic and analytical psychology´s perspective. Subsequently, it briefly mentions prominent psychoanalytics´ fundamental views of this problem. The second theoretical area forms the psychology of art where essential questions of creative art-artist relation (perhaps even spectator) are being presented once more. In addition, not even both theoretical and philosophical approach to creative art should be left out. Pivotal part of thesis is devoted to Tomáš H.´s personality involves artist´s biography, analysis and interpretation of three artifacts on which findings from psychoanalysis and artetherapy are being applied. The thesis´ aim is to confront both latent and manifest content of probed artifacts, along with the interconnection of these findings with artist-mother relation in artist´s early childhood. The final part seeks answers to key questions concerning mother-son-creative production relationships. The thesis´ contribution is seen in artetherapy´s significance affirmation, in its close connection with psychoanalysis and application of artetherapy methods in practice which yields relevant results.
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Preparation of recombinant cytochrome P450 1A1
Dvořák, Martin ; Svášková, Dagmar (advisor) ; Ingr, Marek (referee)
5 Abstract Cytochrome P450 1A1 (CYP1A1) is one of the major isoforms of the cytochrome P450 superfamily. It is primarily an extrahepatic enzyme which is responsible for oxidation of many polycyclic aromatic hydrocarbons and other xenobiotics. Besides of the role in detoxification metabolism CYP1A1 is the one most important isoform involved in activation of procarcinogens. The main aim of this project was preparation of two modifications of the rat CYP1A1 gene with codon optimization for expression in E. coli by gene synthesis. One was wild type (wt1A1) and the other was with modified N-terminal anchor (mod1A1) - for both modifications with or without His Tag at the C-end of CYP1A1. Furthermore, an aim was to compare their level of expression in different strains of E. coli and try to purify and assess enzymatic activity of the gene's products. From pre-prepared oligonucleotides 2 "syntons" (parts of gene) were synthetized and separately inserted into pUC19. After verified sequence of the "syntons" they were cleaved from pUC19 and inserted together into pET-22b. These vectors were prepared for transformation of 3 strains of E. coli (BL-21 (DE3) GOLD, RIL a RIPL). For production of proteins many conditions were tested: temperature (18, 22, 24, 27 a 37 řC), time of production (untill 48 hours), concentration...
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Vylešpení modelu dynamického rozhodování pomocí metody "Iteration spread in time"
Divišová, L. ; Zeman, Jan
In the present work we study the problem of ¯nding the best de- cision based on our previous experience with the system. To solve this task, we use the dynamic programming and its approximations. In the work we summarize the theory needed for usage of the dynamic programming and we deal with its application on futures dealing trying to ¯nd best strategy, id est a sequence of decisions, maximizing our gain or minimizing the loss function. We introduce notion "Bellman function", explain why the approximation of this function is needed, demonstrate one of already tested approximation methods together with its results and we try to propose a method that would lead to the best approximation in suitable time and with available computation aids.
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Constrained activity sequencing
Nguyen, Son Tung ; Barták, Roman (advisor) ; Čepek, Ondřej (referee)
Many scheduling problems can be seen as activity sequencing problems, where the activity sequence in demand satisfies certain constraints. A typical example is scheduling in the airline industry where the task is to assign to each aircraft a segment of flight and nonflight activities while guaranteeing certain required properties. This diploma thesis deals with such type of constrained activity sequencing. The aim is to propose a formal model of the problem, including specification of all constraints and objectives, capable of finding (near)optimal sequences of activities. The proposed model is based on Constraint programming techniques.
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Experimental models of a transfer of stem cells for therapeutic purposes
Faltýsková, Helena ; Krulová, Magdaléna (advisor) ; Indrová, Marie (referee)
Experimental models of a transfer of stem cells for therapeutic purposes Abstract Stem cell therapy currently represents a standard procedure of treating a wide variety of hereditary diseases and serious injuries. Development of the most suitable way of transfer of stem cells into the patient body remains very important question concerning this type of therapy. In our experiments we used nanofiber scaffolds for stem cell cultivation and their subsequent transfer. These nanofibers were prepared by the original needleless electrospun NanospiderTM technology. Allogeneic cornea or skin graft were transplanted from B6 mice to BALB/c mice. The grafts were covered by a nanofibrous scaffold with cultivated stem cells. Stem cells were stained by an imunofluorescent dye to enable us to monitore their migration from nanofibers into tissues and consequent distribution in the body and characterize changes of this distribution in the time. The methods of ELISA and PCR were used to confirm that mesenchymal stem cells support the production of antiinflammatory cytokines IL-4 and IL-10 and contribute to inhibition of production of proinflammatory cytokines IL-1, IFNγ and inducible nitric oxide synthase. We confirmed an important beneficial role of nanofiber scaffolds in transplantation of mesenchymal stem cells. Nanofiber...
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Narrative Memory
Čermák, Ivo
The paper is focused on relations between an autobiographical memory and a narrative self. The author explains how narrative reasoning configures and refigures our incoherent experience, how it constructs language and order of the story, how this kind of reasoning helps to create meaning of the life-story events. He tentatatively conceptualizes a narrative memory and narrative memories which form and content enables this memories to be retrieved as a story or its sequences. The story-telling is conceived as an act of imagination, which is necessarily involved in the process of personal identity construction. Autobiographical and narrative memory is precondition of this process. The study is also an attempt to differ narrative and autobiographical memory.
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Investigation of the β-N-Acetylhexosaminidase Stucture from Penicillium oxalicum.
Krunclová, Tereza ; Novák, Petr (advisor) ; Hýsková, Veronika (referee)
in English β-N-Acetylhexosaminidase (EC 3.2.1.52) is exoglycosidase, which exhibits the unique properties in the filamentous fungi. Enzyme from these organisms are dimeric, inducible and secreted extracelluary. It is expresed as preproprotein, consists of a signal sequence, a large propeptid and a catalytic subunit. Although the enzyme is widely distributed, its structure differs in varies organisms. Bacteria have only monomeric hexosaminidase. Human enzymes are dimeric as well as fungal, but only hexosaminidase from filamentous fungi have the catalytic subunit noncovalently associated with the propeptide. Propeptide is a essential for the enzyme activity. It exists a homologues model of the catalytic subunit of β-N-acetylhexosaminidase from Penicillium oxalicum, but the structure of the propeptide has not yet been solved. The first part of this diploma thesis deals with the optimization of production and purification conditions. The second part deals with structural studies of β-N-acetylhexosaminidases from the filamentous fungi Penicillium oxalicum CCF 3438. These studies were carried out using chemical cross-linking and high resolution mass spectrometry. The combination of these methods revealed region of the noncovalent interaction of the catalytic subunit with the propeptide.
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Josef Ladislav Píč (The Influence of special Disputs for his private and social Life)
KÖSSL, Jan
The thesis is lined in chronological sequence and dividend into six part, its titles are development of science in 19th century {--} history of archeologic research, childhood and adolescence, looking for the vocational integration, the science activity, special disputes, the meaning of publication and legacy Josef Ladislav Píč. The first part is divoted to the history of archeology by its diffident beginnings to its conversion into the real science. The second part treats of birth of Josef Ladislav Píč in 1847, his childhood in Mšeno, Česká Lípa and the years during his grammar schoul attendance in Prague and formation of his ideas. The next part is divoted to the adulthood of Píč and looking for his social enlistment. In the 5th part it is written about his individual science activity. The last but one chapter records his long-term science disputs, which leaded into his early death. The final part sums up his contribution to the science and the legacy of his life´s work.
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