|
Macrophages in leishmania - sand fly - host interaction
Kratochvílová, Tereza ; Kolářová, Iva (advisor) ; Fialová, Anna (referee)
Sand flies (order Diptera) are vectors of Leishmania parasites (Trypanosomatida), which are inoculated into the host skin together with the vector saliva. Sand fly saliva plays the important role in the Leishmania transmission; in naive host it supresses the host immune response assisting Leishmania to establish the infection, while in repeatedly bitten host it elicits a protective immune response. The submitted thesis focuses on the effect of sand fly saliva on macrophages, the key cells in the infection control. In the first part of the thesis we established a laboratory model L. major - P. papatasi - Balb/c to describe the protective effect of saliva immunization on Leishmania infection development. Immunized mice were protected against Leishmania infection which was reflected in the ear lesion size, parasite load in the ear dermis and draining lymph nodes but also in cytokine production. On the contrary, produced lower amount of nitric oxide, while arginase activity was comparable with nonimmunized group. The IgG antibodies against saliva served as a marker of exposure to sandflies while IgG antibodies against Leishmania antigens served as a marker of infection severity. The experiments were aimed on the possibility of cross-protectivity in Balb/c mice against L. major between closely related...
|
|
Výběr a implementace open source nástroje pro řízení portfolia projektů
Marek, Jan ; Chlapek, Dušan (advisor) ; Kučera, Jan (referee)
Methods and ways of implementation of changes and innovations in companies through project management are in today's society very well established. There exists methodologies, techniques and tools for the management of individual projects. However in a role of project manager, I very often faced the fact that companies are performing the project portfolio management in very intuitive way. This in itself leads to failed and prematurely terminated projects, initiation of the wrong projects or realisation of correct projects, but at the wrong time. Very often I have also recognized, that there is lack of awareness of fact, that there are also Open Source applications that can help with the organization's portfolio. This thesis deals with the definition of requirements, search and selection of proper OSS application and subsequent implementation. In the first part of this thesis I prepare a theoretical framework about PPM, and on that basis then identify and verify a set of requirements for selection of right OSS PPM application. The next section of thesis describes searching for suitable applications from a variety of sources, assessment against the requirements and the final selection. In the last part the implementation project is drafted, which aims to serve other colleagues in project management as one of the possible implementation paths. The outputs are then continuously confronted with experts in matter of projects and project management in the field of IT in order to maximize symbiosis between theory and real life experiences. Virtually every project management methodology defines the collection of already-proven techniques, best practices or lessons learned from the previous similar implementations. The main contribution of this thesis I see in fact that it contains not only the design of the project implementation, but also describes a logical path, what leads to the result. Therefore this thesis could be used as a base or discussed best practice, when a project of implementation of PPM application in place.
|
|
The Oriental Institute in Prague: Cultural Department and the Orient
Jůnová Macková, Adéla
A substantial contribution to Oriental studies was founded with a new institution, the Oriental Institute. The idea was promoted by the Czechoslovak president T. G. Masaryk and by his advisor in Oriental politics, Alois Musil. The institute was financially supported by the ministry of Foreign trade, the main role played Minister Rudolf Hotowetz (first director of the Oriental Institute). The Oriental Institute was established in 1922 and financially and functionally stabilised in 1927/1928. There were no permanent salaried posts; however, the cultural section of the Institute financed publications and travel by means of fellowships and grants.
|
|
Health care professional as a patient
Uhlířová, Lucie ; Pečenková, Jaroslava (advisor) ; Ulrychová, Hana (referee) ; Švecová, Dagmar (referee)
The bachelor's thesis deals with the topic of a health care professional dealing with personal experience in a patient's role. The theoretical part presents facts associated with health and ilness psychology, as well as pieces of knowledge about human personality and a man's role in human society. It describes the special demands of general nurse's profession on a person. Special attention is paid to a situation when a nurse becomes a patient. The research part analyses the influnce of nurse's profession over her personal health and ilness perception. It describes difficulties that are experienced when a nurse is coping with her own hospital admission. The main goal of the thesis is to find out whether personal experience in a patient's role influences nurse's future life in any way.
|
|
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
|
| |
| |
|
Miřenka Čechová
Janečková, Kateřina ; PETIŠKOVÁ, Ladislava (advisor) ; SOPROVÁ, Jana (referee)
Monographic work focuses on artistic creation Miřenka Čechová, one of the
the leading personalities of our contemporary stagecraft. Her personal and
artistic development started when she began studying dance and continued to
mimic and physical theater. Material presents her contribution to
contemporary Czech scene physical theater and refers to her international
success. Work observes her role like co-founder of physical theaters Theatro
Pantomissimo and Spitfire Company and to presents the work of these
physical theaters and reveals the working and teaching methods in the
preparation of copyright performances.
|
|
Serge Reggiani, the importance of inconspicuousness
Hrzina, Václav ; VALAK, Radim (advisor) ; Slavíková, Nataša (referee)
I would like to understand the importance of an actor in a movie. I choosed Serge Reggiani, a french actor, who never became a star number one. Reggiani went through a lot of important movies in principal or small roles. What it really means acting in a movie, what brings freedom to an actor in front of a camera and is one actor able to play different characters in every other movie. A diversity and length of his filmography, different directing styles he went through will help me to concretize attitude of an actor and perception of his „different“ characters by audience. I would like to bring up some acting metodes in cinematography in my work too.
|
|
Mask, Disguise and Role Play in Worldwide Contemporary Self-Portrait
Helfertová, Žofie ; SILVERIO, Robert (advisor) ; DVOŘÁK, Tomáš (referee)
This bachelor thesis deals with the role of mask, disguise and playing with the roles through self-portrait in worldwide contemporary photography. In the first chapters it follows the general development of the self-portrait and a brief history of photography and its forms and tendencies from beginning to the present, it is also focused on the mask in general and role of masks and disguises in the history of photography. Main part of this work is the last chapter, which is devoted to the youngest generation of contemporary photographers. In their self-portraits we can follow tendency which reveals use of mask and disguise for change of their identity. However, they do not create a new identity but takes over, or form the illusion of existing already defined identities. The aim of this thesis is to present the mask as an instrument for role play through a self-portrait.
|
guest ::
