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Measuring phonological awareness – blend phoneme test in the beginning of schooling: an comparison of classical test theory and item response theory
Gorčíková, M. ; Šafr, Jiří
The main purpose of this paper is to show dis/advantages of two approaches classical test theory (CTT) and item response theory (IRT) to assess the level of diagnostic tools for evaluating phonemic awareness among first-grade students using longitudinal survey of children during the first grade of primary school (25 schools in Prague and Central Bohemia, 439 pupils). Phonemic awareness (FA) was measured by the task blend phonemes into syllables and words. The advantage of IRT compared to CTT is primarily a concept of independence of the test items. The probability of a response is dependent on the level of latent ability and characteristics of items. So if we know the characteristic of items parameter (difficulty) it could be estimated from the pupil´s responses the level of language skill for diagnostics. The results showed the need for blend phoneme test modifications (in particular for the end of the first grade) so as to adequately measure the high growth of FA ability levels during the first grade.
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Remote sensing as support tool for agricultural drought assessment
Hlavinka, Petr ; Semerádová, Daniela ; Balek, Jan ; Žalud, Z. ; Tadesse, T. ; Hayes, M. ; Wardlow, B. ; Trnka, Miroslav
Very important information about vegetation condition within wide areas (through continents and states) or for local areas in resolution from hundreds to tens of meters could be obtained from satellites within remote sensing. The temporal and spatial continuity is big advantage of this method. Namely so-called vegetation indices are often used for vegetation cover condition assessment. The aim of submitted study is to present possibility of using EVI2 (Enhanced Vegetation Index) for assessment of drought impact within vegetation. The results for selected years of the period 2000-2015 achieved using MODIS (Moderate Resolution Imaging Spectroradiometer) aboard Terra satellite are included. The data in weekly time step and for the whole Czech Republic are presented.
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Biochemical methods as tool for study of reproductive proteins
Postlerová, Pavla ; Zigo, Michal ; Pohlová, Alžběta ; Jonáková, Věra
Study of molecular mechanisms in reproduction is essential for the understanding of this outstanding process. Our lab studies proteins secreted by reproductive organs and sperm using various biochemical methods for a long time. We have expertise in protein extraction from spermatic cells using different approaches, and by kits for proteins from the sperm surface and distinct subcellular compartments. The proteins of reproductive organ fluids are separated by chromatographic methods, such as size exclusion chromatography, high-performance liquid chromatography with reverse phase (RP-HPLC) and affinity chromatography on matrices with various ligands. Proteins are subjected to SDS- or 2D-electrophoresis for their characterization and comparison of various extraction methods, different mammalian species, and sperm in different functional development. Electrophoretically separated proteins may be transferred onto nitrocellulose membrane (Western blot) for antibody detection or binding studies with lectin-labelled ligands (lectins, polysaccharides, zona pellucida glycoproteins). We use immunoprecipitation method with specific antibody for protein determination followed by the MALDI identification. Proteins are localized by immunofluorescent techniques on/in spermatic cells and tissue sections of reproductive organs. Isolation of proteins from reproductive tissues and fluids, and the antibody detection is crucial for the studying of reproductive protein origin.
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Účinky xenobiotik na oxidační stres, metabolizmus lipidů, integritu DNA a životaschopnost lidských buněk a rybích spermií in vitro
LINHARTOVÁ, Pavla
Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing a serious threat to the survival of aquatic organisms including fish. In addition balancing risks and benefits of fish consumption is nowadays an intensively discussed public health topic. Spermatozoa of almost all fish species are released into water environment where they can be directly exposed to various compounds, such as xenobiotics including toxic metals, prior to fertilization. In addition, exposure of parental adults to various xenobiotics may affect gamete quality, which may subsequently reduce fertilization success. On the other hand the advantages of eating fish are well-known, not only in the point that fish is a healthy source of protein and other nutrients, but eating contaminated fish may also confer various health benefits. Research over the past few decades has shown that the nutrients and particularly the n-3 fatty acids (FA) found in fish and seafood, are for examples protective against cardiac diseases and have a positive impact on brain development. The thesis provides a focus on two different cell model types. Firstly, human hepatocellular cells (Hep G2, ATCC) were used as in vitro tool for studying the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, oxidative stress and fatty acid and phospholipid class compositions. Secondly, spermatozoa of one threatened species of fish, sterlet (Acipenser ruthenus) were used as in vitro model for studying effect of potentially hazardous xenobiotic compounds' occurring in open waters. Sperm from sterlet were exposed for 2h to environmentally relevant concentrations of DQ (0-150
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Marketing comunication
Sargsyan, Dianna ; Štůsek, Jaromír (advisor) ; Ladislav , Ladislav (referee)
This diploma thesis deals with marketing communication of the company Top Hotel Praha.
The theoretical part of the thesis is based on the literature research. In the beginning there is a description of what marketing means and what are its instruments, afterwards the thesis gives an idea about the various marketing communication instruments and everything connected with it. Marketing communication instruments include advertising, personal selling, direct marketing, public relations, sales promotion and on-line communication.
The practical part of the thesis describes the company briefly. The diploma thesis also analyses the marketing communication instruments used for the hotel in particular. Marketing communication instruments are examined through the knowledge of the hotel environment. At the end of the practical part of the thesis the author suggests forward steps for marketing communication for Top Hotel Praha as a result of the former findings in the thesis.
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Marketing comunication
Vašáková, Martina ; Štůsek, Jaromír (advisor) ; Marie, Marie (referee)
The thesis deals with marketing communications of a non-governmental,
non-profit organization NaZemi, which contributes to the development of Fair Trade
in the Czech republic. The thesis is divided into theoretical and practical part.
In the theoretical part is defined marketing, marketing mix, and various tools
of marketing communications, which includes advertising, sales promotion, public relations, personal selling, direct marketing, events and experiences.
Part of the theoretical part is characteristic of marketing research, modern trends
in marketing communications and outline of the concept of Fair Trade.
In the practical part is introduced the organization NaZemi with its tools
of marketing communication. Based on the survey findings are formulated proposals, leading to possible improvements in marketing communications. In the end there
is deeply descibed one proposal in detail of a particular campaingn.
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Assessment of Selected Economic Instruments EU in Environmental Policy
Ševc, Pavel ; Spiesová, Daniela (advisor) ; Petra, Petra (referee)
The thesis dealing with the environmental policy on general level, e.g. define the basic terminology as policy of environment, it´s tools, and describe principals of environmental policy. The thesis evaluates ecological taxis and their implementation into Czech legislature, describing realization of tax reforms in Czech Republic and comparing the system with other countries of European Community. The text informs about different math models used in impact evaluation of economic tolls in the sphere of environmental policy. The impact of the selected tools on the state budget of the Czech Republic is analysed in the text. Ecological taxes (solid fuel tax, natural gas tax, other types of gas tax, and electricity tax) create chosen economic tool that is incorporated into Czech legislature. The analytical part of the thesis concerns on analyse and evaluation of the state budget income originated from ecological taxes in the period between 2008 and 2015. Further on the impact on ecological taxis on GDP in different countries of European Community is searched. How ecological taxes contribute to environmental protection is specified in the thesis as well.
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Tool to assess the landscape connectivity in different area units and dispersal distances
Chrumko, Adam ; Šímová, Petra (advisor) ; Krčílková, Šárka (referee)
The purpose of this diploma thesis is to introduce a basic summary of landscape connectivity calculations mainly focusing on the Conefor program and the effect of input parameters on output landscape connectivity indices. One of the main goals is to create an ArcMap Toolbox to simplify the calculation of landscape connectivity indices for many areas of interest. Basic squares that cover the whole Czech Republic were chosen as main areas of interest. These units are used for example for mapping the abundance of flora and fauna. The same indices were calculated for NUTS3 and LAU1 areas in order to compare effect of size of input areas. This thesis provides a simple solution in the form of a Python Toolbox for Esri ArcMap to calculate overall indices of landscape connectivity through a Conefor command line application for a large number of input areas. Forest connectivity for different dispersal distances and input areas of interest was calculated with this Toolbox. As an input vector layer of forests, CORINE land cover data from mapping period 1990, 2000, 2006 and 2012 were used. Input dispersal distances were chosen in the range from 50 to 50.000 meters in order to comprehensively describe their effects on indices. Resultant forest and connectivity changes are represented by cartograms; the effect of input data on output indices is shown in graphs.
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Electronic auction in healthcare
Vyoral, Marek ; Toth, Štefan (advisor) ; Jan, Jan (referee)
Working with the theoretical part focuses on the regulation of electronic auctions in terms of legislation both Czech and foreign, given that we are members of the European Union. Furthermore, the work explains the possible ways to use electronic auctions, which are mostly used in both the Czech Republic and abroad. At work I also am trying to shed light on the current use of auctions on the auction portal Aukro examples, which is widely used by people for personal use. Finally, the theoretical part I try to outline the future development of electronic auctions. The practical part is focused primarily on its activities in the hospital Pardubice Region, Inc. where I introduced the auction. In several examples, visible results, which not only brings centralization entire purchase, but also the use of electronic auctions as a tool for communication and bidding suppliers with the lowest purchase price for a group of hospitals. The work also draws from the Ministry for Regional Development, which is responsible for the preparation of the most important regulations for electronic auctions in healthcare, namely the Public Procurement Act. Electronic auctions are a tool very interesting, because it is the most transparent way of evaluating procurement by parameters rates. Whether if electronic auction in health care and efficient purchase option will be discussed in this work.
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Virtualisation tools in theory and practice
Svozil, Marko ; Halbich, Čestmír (advisor) ; Lohr, Václav (referee)
The main objective is to characterize the different types of virtualization approaches and introduce major virtualization technologies and tools. A partial aim of the thesis is to compare the ability and potential of virtualization tools from VMware and Microsoft on the basis of an environment analysis, infrastructure requirements and possibilities of the sample company and implement the appropriate virtualization solution.
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