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Cell culture-based model for the evaluation of adhesive properties of probiotic bacteria
Theodorou, Vasiliki ; Havlík, Jaroslav (advisor) ; Tauchen, Jan (referee)
Probiotic microorganisms, defined as living microorganisms which when administered in adequate amounts confer a health benefit to the host, and their adhesion and colonization of intestinal epithelium, are critical factors in maintaining probiotic efficacy. Polyphenols are a large and heterogeneous group of phytochemicals in plant-based foods, such as tea, coffee, wine, cocoa, cereal grains, soy, fruits and berries. In the last decade, there has been much interest in the health benefits of dietary plant polyphenols that arise from their potential ability to promote adhesion of probiotic bacteria to the human intestinal epithelium.
The purpose of this study was to investigate the effect of four polyphenols: isoquercetrin, phloretin, procyanidin B2 and rutin on the adhesion ability of two potentially probiotic strains (Lactobacillus casei, Lactobacillus gasseri) to in vitro human intestinal epithelial model consisting of Caco-2 and mucus-secreting HT29-MTX co-culture.
The adhesion of Lactobacillus casei after treating the co-culture cell lines with isoquercetrin, phloretin, and rutin was increased by 49.76, 72.97, 63.66 % respectively, whereas procyanidin B2 inhibited the adhesion 20.25% compared with the control sample. The adhesion of Lactobacillus gasseri after treatment of the co-culture with isoquercetrin, phloretin, procyanidin B2 and rutin was increased by 35.45, 31.28, 45.69, 25.01 % respectively compared with the control sample.
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Potential of sustainable development of the Village
Uhlíková, Ivana ; Varvažovská, Pavla (advisor) ; Marcela, Marcela (referee)
This thesis analyzes the sustainable development strategy focusing on the environmental pillar and the subsequent evaluation of the implementation of environmental sustainability in Kladno. The main aim was to evaluate the active and environmental policy perspective of the city.
In the theoretical part are described various laws, documents and instruments relating to environmental policy and sustainable development. The thesis is talking about the area of interest, its historical development, demography, environment, social and cultural fields.
Using concrete examples it is recommended how the environmental management of Kladno could proceed in the future. The proposed recommendations are a result of analysis of all available information which include the needs and requirements of city residents.It is important to update Strategy of environmental policy and introduction of activities from the Sustainable Development Strategy SO ORP Kladno in to practise. Improving of public services and community cohesion. Establishment of school forums in primary schools.
The final part contains the results of a field survey. Questionnaires were handed and collected in combination with controlled interviews.
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The growth of Lodgepole Pine (Pinus contorta) in reclaimed spoil banks
Štrudl, Robin ; Bažant, Václav (advisor) ; Slávik, Martin (referee)
This diploma thesis deals with the detection of the dynamics of the growth and development of lodgepole pine, which is intended for the purposes of the forestry reclamation on anthropogenic soils spoil banks. In the first stage it is approached the issue of growth and development of woody plants on spoil banks. Are described recent formations, their cutting and editing, further forestry reclamation and associated issues, on which follows the description of the entitlements of tree species in different territories, further criteria necessary for the correct evaluation of the conditions on the habitats directive and appropriate ways of establishing the stands. Subsequently, we approached lodgepole pine, its description, extension, variability, etc. In the second part of the thesis is studied and evaluated growth of lodgepole pine. The first is characterized by the interest of the territory and its natural conditions. On the plots in the forestry arboretum on the spout Antonin samples were taken in the form of stem discs, which served as the basis for determining the necessary values. Values were obtained from tree rings.
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Life cycle and documentation of student projects
Zbořilová, Nikola ; Bartoška, Jan (advisor) ; Mudrychová, Kristýna (referee)
The aim of this work is to describe and compare student projects by time, range, sources, benefits, risks and quality and suggest possible ways of improvement in planning and organizing of these projects. First part of the work introduced theoretical overview in area of project management and the terms used in case study are listed and described. At first, there are described obtained information about specific projects that are the subject of comparison. From this information was analysed the state of use of the project management tools and the life cycle of student projects. This analysis shows information important to following comparison. Then, it was examined which factors positively or negatively influences the planning and organizing of projects. Subsequently, the proposal was drawn up to enhance the positive effects and reduce negative impacts on student projects. As an additional study then came the analysis of students' interest in participating in the Contest of Student Projects organized by the Department of Systems Engineering at the Faculty of Economics and Management of the University of Life Sciences in Prague.
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Analysis of tourism in rural region Moravice and proposal of its environmentally friendly development
Manderlová, Jana ; Kašparová, Ivana (advisor) ; Hana, Hana (referee)
The thesis is focused on environmentally friendly tourism. Defined area of interest is the country microregion Moravice. The aim of thesis is to map the most interesting tourist destinations and locations of this microregion, analysis of its facilities of tourism services, evaluation of the current opportunities of tourism and determine its strengths and weaknesses of the services provided to visitors. The used methods for data collection include field surveys, analysis of available books, newspapers and Internet sources, questionnaire survey with visitors, residents and councilors and subsequent discussions with them about the problems. Analysis of the defined area due to the issue of tourism is included in the SWOT analysis, which is the basis for the design of specific projects for the future development of tourism in the microregion Moravice.
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Change trajectories of wood-pastures focusing on changes in management
Čermáková, Kamila ; Skaloš, Jan (advisor) ; Pavel, Pavel (referee)
This thesis is focused on monitoring changes in the trajectory of wood-pasture mainly in changes in management. The main goal of research was land-cover according to the Imperial prints and current orthophoto maps. Area of interest is located in the Stredocesky and the Ustecky region, specifically my intereset was six historic regions in the Midwestern Bohemia, which had the largest landscape heterogeneity.
The first part describes the historical development of wood-pastures, their definition and the current status. Information was obtained from the Czech and foreign literature. Another part deals with specific trajectories in the area of interest. Changes in wood-pasture from mid-19th century to the present day were compared by program ArcGIS. In conclusion you can find the evaluation of the results by graphs, tables and verbal evaluation of the likely causes of changes in trajectory.
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The use of unmanned aerial vehicles in agriculture.
Starý, Karel ; Kroulík, Milan (advisor) ; Petrásek, Stanislav (referee)
Unmanned aerial vehicles has been a fast developing field over the last couple of years Farmers have been trying to take profit from this development and are interested in various options of applications. Demands on the area of agricultural production have been increasing, especially higher effectivity and the economical aspect of production are in focus. Farmers seek for the information about crop statuses and cattle in pursuit of economical rentability and enviromental sustainability. Due to this awareness farmers can take measures to reach economical profit. In the form of literature review the thesis describes construction types, their power options and the suitability for particular application is evaluated. Furthermore, scanning varieties and particular examples of applications are mentioned.
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Budget of the European Union in the field of justice and home affairs
Ticháček, Jan ; Hřebík, František (advisor) ; Markéta, Markéta (referee)
The Master Degree Thesis focuses on analysis of the EU budget. The main area of interest is justice and home affairs. Planning of the EU annual budget is preceded by creation of the Multiannual Financial Framework which is a planning tool and also a safeguard of predictability of the EU expenditures. Also EU priorities are mirrored there. Annual budget itself is divided into six headings. The thesis is dedicated mainly to the third area Security and Citizenship. Also an EU agency CEPOL (European Police College) is financed from this area. The second part of the thesis is dedicated to CEPOL. The EU agency CEPOL occupies important position in the field of education and training of senior police officers and also law enforcement officers. It provides educational and training activities which suppose to contribute to improving of EU Member States´ cooperation in fighting against the most serious forms of international crime. The flagship of CEPOL is European Police Exchange Programme. Thanks to the exchange programme, the participants gain experience based on their needs and preferences. CEPOL supports qualitative rather than quantitative education. The third part of the theses tries to find out whether the financial resources spend on the Exchange Programme are used effectively. This issue is examined by using a questionnaire and subsequently its evaluation.
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Support of regional development through the Local Action Group Podchlumí
Jezberová, Zuzana ; Hejnák, Václav (advisor) ; Marcela, Marcela (referee)
The Diploma thesis deals with issues of the support of regional development through the Local Action Group Podchlumí, r.a. (LAG Podchlumí).
The goal of this thesis was to explore the contribution of the LAG Podchlumí to quality of life of residents and business development. Assessment of the influence of LAG on regional development has been based on the existence and implementation of the Program of rural development program of the Czech Republic Axis IV. LEADER, which leads to an improvement of management and to mobilization of the natural inner potential of countryside. The Program of rural development represented the main financial source for fruition of LAG projects in the previous programming period.
In this thesis, activities of the LAG Podchlumí and local subjects for support and development of partnership of residents and for public, business, and non-profit communities have been analysed. Implementation of own projects, through which the versatile development of the region, life improvement of residents, increase the attractiveness of the region has been evaluated. In order to get an independent and objective evaluation, an anonymous questionnaire has been conducted among residents of the region Podchlumí. Further, personal observation of the region Podchlumí, a study of professional literature as well as of internal materials of the LAG has been done. The hypothesis that the Local Action Group Podchlumí plays an important role in development of partnership of public administration of municipalities, agricultural subjects, independent agricultural as well as non-agricultural enterpreneurs, and non-profit organizations has been verified on the basis of analysis of realized activities. Support from the Program of countryside development to the region Podchlumí, Measure IV.1.2 Realization of local development strategy, contributed to achieving improvements in quality of life of residents. The greatest support was given to appearance of communities and quality of infrastructure, development of public facilities and services. Financial resources were spent also on modernization and development of agricultural as well as non-agricultural business. In comparison with other LAGs in the district Jičín, the LAG Podchlumí supported the greatest number of projects through the measure IV.1.2 of the Rural development program on realization of local developing strategy. It has been found by means of evaluation of the anonymous questionaire among residents of the region that residents perceive improvement of appearance and life of communities but they are not aware of the fact that many projects have been implemented with the contribution of activities of the LAG Podchlumí. For this reason, the LAG Podchlumí should focus more on propagation of its own activities. Residents of the region are interested in surrounding of their villages.
It is possible to made conclusion that the results of analysis of activities of the LAG authenticated and confirmed correctness of the hypothesis that the Local Action Group Podchlumí plays an important role in development of partnership of public administration of municipalities, agricultural subjects, independent agricultural as well as non-agricultural enterpreneurs, and non-profit organizations.
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A systém of field and forest roads in the administrativ area Suchovršice
Jansa, Petr ; Zilvar, Josef (advisor) ; Petr, Petr (referee)
This thesis deals with the network of field and forest in the cadastre unit of Suchovršice and suggest to supplement the network of unpaved roads.
In the theoretical part is summarized the history of the field and forest roads. Then there is a division of field and forest roads according to the relevant standards ČSN. Another section is devoted to maintenance events, repairs and reconstructions of field and forest roads. There are outlined the possibility of financing the construction of new roads. Follow a description of the most important objects on the field and forest paths.
In the practical part is defined area of interest including the broader territorial relations. Then there are described the current field and forest roads in the area of interest, which were plotted to the map of road networks. This map is the result of performed detailed field research. On the basis of digitized map stable cadastre is described the road network in 1840. Due to the fact could be compared to road networks in two different time stages. The existing system of field and forest roads is evaluated. For ratings are calculated densities of both types of roads in the area of interest. The calculated density of the field and forest roads are assessed with an average density of roads in the Czech Republic. The density of field and forest roads in the selected area is higher than the national average. This can be explained by the type of relief which corresponds hilly land type. Overall, the number and length of roads is sufficient. However, density of forest haul routes is insufficinet. Problematic is also the technical condition of rural roads in the selected area.
Based on the analysis of historical and current road network is designed to complement the rural roads within the land adjustment. Land adjustment just underway, and draft routes can be used to compile Plan of common facilities.
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