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From Descates' Mind to Kant's Reason
Dekastello, Petr ; Hill, James (advisor) ; Palkoska, Jan (referee)
The basic subject-matter of my research is R. Descartes' Meditations On First Philosophy and I examine the term intellect and its position within the mind and its relation to thought. My work includes a study of sensation and imagination which provide the intellect with "material" to judge. The objective of my thesis is to demonstrate the role the intellect plays in terms of reason and to present an interpretation of the way in which the mind is designated in relation to reason according to Rene Descartes in his writing and in his responses to objections. The position and functions of the intellect are understood as a logical structure of mutual dependence and as the elements essential for the understanding of the human soul substance. At the end of my thesis I demonstrate that the system of reason of R. Descartes might be considered as the basis for the transcendental conception of reason in Kant's philosophy, which leads to an idealistic world conception and is founded on thinking a priori. Powered by TCPDF (www.tcpdf.org)
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Activation of carcinogens in gastrointestinal tract
Zawadová, Dorota ; Hodek, Petr (advisor) ; Koblihová, Jitka (referee)
The Bachelor Thesis deals with an activation of heterocyclic aromatic amines (HAA) which have numerous carcinogenic effects on studied animals and human cells. These carcinogens are formed during the heat processing of meat and during the smoking. However, further transformation of the compounds is required to gather their carcinogenic effect. Most of all HAA are first activated by cytochrome P450 (CYP), especially its forms 1A1 and 1A2. The products of this activation - N-hydroxylamines - are further activated in conjugation reactions. In this work, we were focused on the transformation of N-hydroxylamines to more reactive acetate esters and sulphate esters, which is catalyzed by sulphotranspherase (SULT) and N-acetyltranspherase (NAT), respectively. The affection of these enzymes can control the formation of carcinoma. For example, some dietary compounds, such as caffeic acid and quercetin, are the most common inhibitors of these enzymes: caffeic acid is considered as a strong inhibitor of phenolic sulphotranspherase (P-PST), whereas quercetin is a good inhibitor of NAT. On the other hand, some dietary compounds can also induce an opposite effect: for instance, phenol acids induce the P-PST. (in Czech) Key words: Heterocyclic aromatic amines, biotransformation, cytochrome P450,...
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Interaction of selected flavonoids with cytochromes P450 2B
Boštíková, Zdislava ; Hodek, Petr (advisor) ; Kubíčková, Božena (referee)
In recent years, there has been increased emphasis on healthy lifestyle, which includes, among others, consumption of natural nutrients in a form of various food supplements. These compounds, usually of a flavonoid character, show different chemopreventive effects such as anti-oxidant or anti-carcinogenic. The excessive consumption of these compounds can also have a negative impact on human health. Flavonoids may for instance influence the content of biotransformation enzymes, cytochromes P450, in the cells and thus influence the metabolism of foreign compounds. Such an influence might, as a result, lead to a development of carcinogenesis or inefficacy of certain medications. The main focus of this bachelor thesis is to study capabilities of two flavonoid compounds (myricetin and dihydromyricetin) to induce and inhibit cytochromes P450 of 2B family. By the Western blot method it was found that neither of the investigated flavonoids increased expression of CYP2B1/2 in livers, myricetin, or in any part of small intestine. In the case of dihydromyricetin there was noticed an induction of cytochromes P450 2B in the middle part of small intestine. The inhibitory potency of flavonoids was examined in vitro as an inhibition of 7-pentoxyresorufin O-depentylation catalyzed by CYP2B. Values of IC50 8,8μM and...
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The effect of dihydromyricetin and myricetin on selected biotransformation enzymes
Boštíková, Zdislava
Flavonoids are natural compounds commonly ingested in herbs and vegetables. They are believed to have a positive impact on human organism, in particular by their antioxidant, hepatoprotective and anti-cancer effects. In these days, it is possible to consume high concentrations of these compounds in form of dietary supplements. However it is not clear, whether flavonoids in such unnaturally high concentrations are still beneficial or rather harmful. It has already been proven, that flavonoids can influence the activity of biotransformation enzymes and interfere e.g. with the process of carcinogenesis and drug metabolism. For that reason it is important to investigate the impact of an increased intake of flavonoids. The aim of this thesis was to investigate the influence of dihydromyricetin (a potential drug to cure alcohol use disorder) and its structurally similar flavonoid myricetin on the activity of enzymes, cytochrome P450 2E1 (CYP2E1) and Nacetyltransferases 1 and 2 (NAT1/2). The research included the determination of the impact of a premedication by these flavonoids on the expression and activity of CYP2E1 and NAT1/2. The inhibition capacity of myricetin and dihydromyricetin towards the activity of CYP2E1 and NAT1/2 was also investigated. After the flavonoid premedication of rats the...
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Study of the effect of roscovitine and its derivatives on the expression of drugs transporters in vitro
Mlčochová, Alice ; Čečková, Martina (advisor) ; Carazo Fernández, Alejandro (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Mgr. Alice Mlčochová Supervisor: doc. PharmDr. Martina Čečková, Ph.D. Title of rigorous thesis: Study of the effect of roscovitine and its derivatives on the expression of drugs transporters in vitro In this study we aimed to evaluate interactions of selected inhibitors of cyclin-dependent kinases (CDKi) with efflux transporters ABCB1 (P-glycoprotein, P-gp, MDR1) and ABCG2 (breast cancer resistance protein, BCRP) in cell line LS174T derived from human adenocarcinoma of colon. Among the selected compounds belong roskovitin and its derivates designed as BA-12 and BP-14. Previous studies demonstrated that these substances inhibit both mentioned transporters and therefore increase the effect of simultaneously administrated cytotoxic drugs. Since the drug interactions on the level of transporters can arise from induction of their corresponding genes, the aim of this work was to extend the current knowledge on mentioned derivates of roskovitin in terms of possible affection of ABCB1 and ABCG2 expression. Enhanced expression could promote developement of multidrug resistance (MDR). Results of this work obtained with qRT- PCR method show that neither BA-12 nor BP-14 increase expression of...
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Electronic RLC meter
Dvořák, Radim ; Hanák, Pavel (referee) ; Lattenberg, Ivo (advisor)
This work deals with measurement methods of passive components R, L ,C used almost in every electrical circuit. Work describes progress of choosing the right methods for measurement and also the process of designing the autonomous RLC meter with use of ATMEL microprocessor chips. The RLC meter will be powered from rechargable battery and will be able to measure primary attributes of RLC components.
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Design of a high-speed electrical machine.
Šolc, Martin ; Klíma, Jiří (referee) ; Mach, Martin (advisor)
This diploma thesis is dedicated to design of highspeed induction motor with power 30 kW, 60 000 rpm. In the beginning of the thesis basic problematic of highspeed motors, used materials and motor types is discussed. The practical part of the thesis concerns design of the motor itself, creating a model in ANSYS Maxwell software, performing several simulations and optimalization of the designed machine. In the last parf of this thesis each simulation is evaluated and the final model of the machine is introduced.
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The effects of endocrine disruptors on the expression and the activity of cytochromes P450 2B in laboratory rat as a model organism
Měkotová, Barbora ; Dračínská, Helena (advisor) ; Levová, Kateřina (referee)
Endocrine disrupting chemicals are compounds that interfere with natural hormones and they are responsible for functional changes which may lead to damage of the endocrine system. Their presence in the environment is associated with a number of diseases whose extent is hard to predict. As endocrine disrupting chemicals, a wide range of exogenous and endogenous compounds is present in the environment. Important exogenous endocrine disrupting chemicals include benzo[a]pyrene (BaP) and 17α-ethinylestradiol (EE2); the female sex hormone 17β-estradiol (E2) can act like endogenous endocrine disruptor. In this thesis, the effect of these three compounds and their combinations on the expression and the activity of rat biotransformation enzymes cytochromes P450 2B is studied. The gene expression was determined by quantitative PCR, the expression of the protein itself was studied using Western blot method and consecutive immunodetection. The results show that CYP2B expression is almost unchanged after BaP premedication, whereas estrogenic compounds, E2, EE2, their combination and their combinations with BaP, significantly decrease the expression. The enzyme activity of CYP2B was also studied in rat liver microsomes using the marker substrate 7-pentoxyresorufin. EE2, E2 and their combination decrease the...
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FEM model and simulation of induction motor with pole-changing winding
Záškodný, Jiří ; Ondrůšek, Čestmír (referee) ; Vítek, Ondřej (advisor)
This master thesis deals with calculations and simulations of multi-speed induction motors. In the first part, basic principle of these machines is described. Next, there are given three examples of pole-changing stator windings and their properties are analyzed (winding factors, magnetomotive force). Main part includes simulations and measuring of the specific motor, which is produced by company Siemens Mohelnice. This is the motor with 2/1 pole-changing in Y/YY connection. First, influence of skewed rotor slots on current and torque is analyzed. Next, parametres of motor from simulations are given and these results are compared to measured values.
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Wireless charging station
Pokorný, Josef ; Hrabina,, Jan (referee) ; Janoušek, Jiří (advisor)
The paper deals with possibilities of wireless charging and transmission of electric energy using inductive coils. Basic models of air coils are described using CST simulation software. The product consists of a transducer for transmitting coil, two coils with ferromagnetic shield, synchronous rectifier and battery charging circuit. Communication is provided by an 8-bit microcontroller with wireless modules and an ethernet shield for online monitoring. The aim of the thesis is to design a wireless charging station with a power transfer of 40 W with the possibility of online monitoring of the charging state.
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