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MR imaging and MR spectroscopy of human during physical stress (MR spectroscopy imaging, MR diffusometry, MR relaxometry etc.)
Šedivý, Petr ; Hájek, Milan (advisor) ; Haberlová, Jana (referee) ; Vymazal, Josef (referee)
The dissertation is concerned to in vivo phosphorus MR spectroscopy (31 P MRS) and 1 H MR imaging (MRI) of muscle in combination with physical workload. The theoretical part of the thesis describes methodology of 31 P MRS measurement and its clinical use in research of metabolic changes in diabetes, heart failure and peripheral artery disease (PAD). The results of the thesis are divided into methodical and clinical parts. Methodical results deal with the construction of experimental equipment, software modification and development, and show of the reproducibility of the dynamic 31 P MRS. The MRI after exercise was used to the describe involvement of the individual calf muscles to muscle contraction during pedal movement in MR compatible ergometer. The first part of the clinical results of the thesis describes changes in muscle metabolism during diabetes and critical ischemia. In patients with critical ischemia the effect of treatment by angioplasty or transplantation of mesenchymal stem cells was evaluated. In the second part of the clinical results the metabolism of patients with heart failure complicated by sideropenia was studied. In these patients the effect of experimental treatment by iron carboxymaltose was described.
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lmportance of epicardial fat in heart diseases: new mechanism of action and their therapeutic implication
Matloch, Zdeněk ; Haluzík, Martin (advisor) ; Maruna, Pavel (referee) ; Lipš, Michal (referee)
It was documented earlier that epicardial fat is a source of pro-inflammatory mediators under basal conditions (Mazurek, Zhang et al. 2003, Baker, Silva et al. 2006). It was also found that during cardiac surgery there is an increased expression of mRNA for pro- inflammatory cytokines in both epicardial and subcutaneous adipose tissue (Kremen, Dolinkova et al. 2006). During advanced heart failure, an inflammation of adipose tissue and resistance to adiponectin was also discovered (Khan, Kato et al. 2012). As part of the project, we tested the hypothesis that changes in levels of pro-inflammatory and anti-inflammatory cytokines, including their expression in adipose, muscle and heart tissue, as well as changes in insulin resistance and lipid profile parameters, are dependent on the type of underlying disease of patients, either the presence of CAD or valve degeneration. The aim of our project was to investigate metabolic changes in various fat tissues, insulin resistance, pro-inflammatory and anti-inflammatory cytokines plasmatic levels and their mRNA expressions in patients with coronary artery disease (CAD) undergoing either elective coronary artery bypass surgery (CABG) or isolated valve replacement. Our patients underwent several blood tests and measurements like anthropometric, biochemical, and...
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Functions of Neuregulin 4
Majerčáková, Eva ; Žurmanová, Jitka (advisor) ; Haasová, Eliška (referee)
This work focuses on reviewing the function of Neuregulin 4 (Nrg4) and its receptor ErbB4 in various biological processes and disease states, including inflammation, cancer, metabolic diseases, and liver diseases. In metabolic diseases such as diabetes, Nrg4 may affect glucose metabolism and lipid homeostasis, but the exact mechanism of action is not yet well understood. Nrg4 and its receptor ErbB4 appear to be key factors in regulating inflammation and promoting cell survival. Moreover, in liver diseases, such as nonalcoholic fatty liver disease and nonalcoholic steatohepatitis, Nrg4 and ErbB4 seems to play a protective role. Overall, these findings highlight complex roles of Nrg4 and ErbB4 in a multitude of biological processes and disease states. Further research is needed to fully understand these relationships and their potential as diagnostic or therapeutic targets.
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Strategies and results in the development of insulin derivatives for the treatment of diabetes
Pokorný, Štěpán ; Jiráček, Jiří (advisor) ; Hlouchová, Klára (referee)
The aim of this bachelor's thesis is to present insulin analogs and to analyse the direction of development of these organic compounds. The text contains basic information on this subject (e.g. the history of Diabetes Mellitus, the first treatment, the discovery of insulin (1921) and the experiments that preceded this discovery...). The thesis also explains the basic differences between type 1 and type 2 Diabetes Mellitus and the resulting differences in treatment. It mainly summarizes, the specific types of analogs, their chemical composition and the nature of their action. The main contribution of this work lies in the analysis of insulin analogs and the overview of the course in which the development of these analogues is proceeding or what innovations we can expect in diabetes therapy in the upcoming years. The thesis draws primarily from peer-reviewed scientific papers, articles and books (older as well as more recent ones) and from the expertise of the IOCB laboratory. All sources are well cited at the end of the work.
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Possibilities of influencing compliance with following diabetic regimes from the perspective of nutrition therapist
Kučerová, Denisa ; Vachek, Jan (advisor) ; Zakiyanov, Oskar (referee)
Diabetes mellitus is a metabolic disease that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces. Only in the Czech Republic, we detect over one million people diagnosed with this disease. By 2045, over 700 million people are predicted to be diagnosed with diabetes worldwide (Global diabetes data report 2000 - 2045 (diabetesatlas.org)). Medical community and pharmaceutical companies give big importance and pay significant attention to the development of innovative ways of treatment. Nowadays, there is a wide range of treatment available, but only hand in hand with the right compliance and patient's motivation can medical treatment bring the desired outcome. This bachelor thesis aims to explore the level of awareness among diabetic patients about the disease itself and nutrition knowledge through out a questionnaire survey. The main part of the thesis describes factors that can help with the patient's motivation. Questionnaire survey was used in a set of 271 patients treated in Klatovska nemocnice. Results of the survey are presented in the form of graphs. Results of the survey show that men are more satisfied with the ongoing threatment than females. On the other hand, both men and women do not view the level of education...
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Regime of a Diabetic Patient during Hospitalization in the Surgical Ward at IKEM
Slavíková, Petra ; Bakusová, Tereza (advisor) ; Komínek, Tomáš (referee)
The bachelor's thesis focuses on research during the hospitalization of three type II. diabetics at the Transplantation Surgery Clinic at IKEM. The thesis is divided into theoretical and practical parts. The theoretical part describes the anatomy, history of diabetes, diagnosis, possible complications of diabetes, potential therapy, education of diabetics, and nursing care for diabetics. The practical part focuses on independent research of the regime by monitoring the diabetics for two days during their hospitalization. The aim of the thesis is to focus on the precise course of hospitalization for diabetics. The aim is not to control the correct course, but rather to examine the regime that is provided to diabetics on the surgical ward at IKEM. The thesis evaluates the regularity and amount of food intake, blood glucose measurements, insulin administration, and adherence to dietary measures. The thesis describes the individual nutrition of the selected diabetics, with precisely measured blood glucose values and the amount of insulin administered. It also includes final questioning of the patients on specific questions closely related to the regime. The goal of the interview was to identify potential complications that patients suffer from. Keywords: Diabetes Mellitus, blood glucose, IKEM, nursing care
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Diabetes mellitus and breast cancer screening in Czechia in the context of socio-economic factors
Vrchlavská, Eliška ; Kulhánová, Ivana (advisor) ; Brož, Jan (referee)
This thesis focuses on the relationship of diabetes mellitus and participation in mammography screening in women in the Czechia under the influence of socioeconomic status and lifestyle factors, and it is based on the assumption that women with diabetes mellitus participate in secondary prevention of breast cancer less frequently than women without diabetes. Logistic regression analysis methods were used to assess the relationship between participation in mammography screening and diabetes, age, SES (education, household income) and lifestyle (obesity, smoking, alcohol) using European Health Interview Survey (EHIS) data from 2019. The link between SES and lifestyle is assessed for women with diabetes, as well as for women without the diagnosis. The results of this study do not show a statistically significant relationship between diabetes and participation in mammography screening, however, do demonstrate a socioeconomic gradient in participation in screening, both in the diabetes-free and diabetic populations. At the same time, they demonstrate an increased probability of participating in screening for occasional alcohol consumers. Key words: diabetes mellitus, breast cancer, mammography, EHIS, socioeconomic status
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The circulating cell-free DNA as a biomarker of civilization diseases
Dyachenko, Yulia ; Daňková, Pavlína (advisor) ; Vaňková, Markéta (referee)
The incidence of civilization diseases in the population is increasing every year due to changing environmental conditions and modern lifestyles. These diseases reduce the quality of life, lead to health complications or even death of patients. This can be prevented by monitoring health status. Circulating free DNA (cfDNA) has the potential to be introduced as a non-invasive biomarker. Possible mechanisms of cfDNA release are apoptosis, necrosis, NETosis, pyroptosis and active secretion. The occurrence of these processes in some civilization diseases allows the use of cfDNA as a biomarker. The aim of this work was to summarize the current knowledge on the possibility of using cfDNA in the diagnosis, monitoring of the development and course of some diseases of civilization, namely obesity, diabetes mellitus, atherosclerosis, hypertension, myocardial infarction and stroke. From the available literature it can be concluded that cfDNA could be used in clinical practice for the purposes described above. Key words: Circulating cell-free DNA (cfDNA), civilization diseases, biomarker, obesity, diabetes mellitus, atherosclerosis, hypertension, myocardial infarction and stroke.
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Diet at patients after total pancreatectomy
ŠEDIVÁ, Denisa
In the bachelor degree project Diet for the patients after total pancreatectomy I deal with a diet of the patients who underwent surgery called total pancreatectomy. The introduction of the theoretical part is focused on the anatomy of the pancreas and its function. The following part of the project is directly focused on carcinoma, its epidemiology, incidence, cause of the disease and also development. I also deal with clinical symptoms, treatment and pancreatectomy itself. The last part of the project is oriented towards nutrition for the patients after surgery. Further in this section, I deal with pancreatic and diabetic diet and diet during chemotherapy, which many of the respondents have undergone. As the goal of the project, I chose to look into the alimentation of the patients with this disease and to find out how difficult it is to combine three types of diet together. I focused on the observation of the intake of basic nutrients such as carbohydrates, fats, and proteins. The practical part I compose as a qualitative research. There were 6 respondents who took part in the research. Unfortunately, only two of them provided me with their blood glucose values to evaluate their alimentation according to the diabetic diet. The result was very surprising. In relation to the seriousness of the illness, most of them ate basically without restriction. They were eating everything they wanted except sweet dishes, for example cakes and fried dishes. They just watched their blood glucose values. Despite all the experienced weight loss, their current alimentation meets recommendation and no one of them suffers from lack of any of the macronutrients according to the results.
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