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Determination of antimicrobial activity of herbal extracts
Žitková, Barbora ; Veselá, Mária (referee) ; Vítová, Eva (advisor)
The aim of this bachelor thesis was to observe the antimicrobial activity of selected herbal extracts and essential oils against three selected microorganisms, bacteria – Bacillus cereus, Escherichia coli and the yeast Candida glabrata. Two diffusion methods were used to verify and compare the antimicrobial activity, namely the diffusion well and disk method. The theoretical part describes the characteristic of plants from which extracts and oils were made. It also focuses on active substances, vitamins and minerals contained in herbs. Other methods that can be used to determine antimicrobial activity are also mentioned in the work. At the end of the theoretical part the characteristic of the microorganisms used for observation is mentioned. The result of the diffusion well method shows that only eucalypt oil showed antimicrobial activity against all selected microorganisms. It was most effective against Bacillus cereus with an inhibition zone size of 1.73 ± 0.11 cm. The result of the diffusion disk method shows that there are two oils which showed antimicrobial activity against selected microorganisms, namely marrow and eucalypt oil. Nevertheless, the most effective oil was made of mint against Bacillus cereus with an inhibition zone size of 1.57 ± 0.31 cm.
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The Influence of traditional ways of management on the State of Coppice - Results in 2018
Balková, M. ; Dařenová, Eva ; Friedl, M. ; Hloucalová, P. ; Kadavý, J. ; Kneifl, M. ; Knott, M. ; Kučera, A. ; Slach, T. ; Skládanka, J. ; Uchytilová, Tereza ; Uherková, B. ; Vichta, T. ; Volařík, D.
The most pronounced increase in number of herb species was remarked in the clear cut+litter gathering treatment. Harvest led to an increase in soil temperature and moisture. TheCO2efflux was the highest in September, namely in control treatment. The lowest intensity of root growth was remarked in the control treatment. Variants with litter gathering had smaller (28.9%) relative growth ratio.
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Herb garden and its interdisciplinary use for primary school in Borovany
HEBÍKOVÁ, Petra
This diploma thesis deals with the use of herbal garden during teaching at primary school. The thesis include proposals for activities that have appropriate cross-curricular relations. This activities will reinforce the pupils' knowledge in the area of biology, maths, czech language, art and in music. The activities also include worksheets with various instruc- tions and tasks for each point of interest.
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The Effects of Medicinal Plants on the Human Body
Škrdlová, Kamila ; Hlaváčová, Lucie (advisor) ; Skýbová, Jana (referee)
The diploma thesis concentrates on medicinal plants, their use and effects in the treatment of diseases. The thesis summarizes some effects of medicinal substances contained in plants and herbs. They are suitable for use in selected diseases. The research part of diploma thesis deals with the mapping of methods of treatment and production of herbal products among university students. The thesis also examines whether students are inclined to use more herbal therapy over conventional pharmaceutical drugs. It investigated whether information on herbs in schools sufficient. KEY WORDS medicinal plants, plant's medicinal substances, herbs, products, phytotherapy
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Sensory evaluation of drinks enriched with extracts of herbs
Scholzová, Kristýna ; Pořízka, Jaromír (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with sensory evaluation of herbal non-alcoholic beverages, being a part of a new product development. The theoretical part provides information concerning chemical composition, health effects, food applications and aroma compounds of the herbs of interest (peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and lemon balm (Melissa officinalis). Another topic is a description of sensory methods, requirements and arrangement of the analysis, statistical methods, and also technological processes leading to herbal syrups and beverages production. The practical part comprises sensory analysis of a few, newly designed, formulas of one-component and two-component alcohol free herbal beverages. The sensory panel consisted of 40 students of master's and also doctoral study programme at Faculty of chemistry, University of Technology Brno. Four subsequent experiments are introduced, each of them consisting of a few sensory tests and including corresponding sensory forms attached. The sensory tests arrangement was based on currently valid Czech Technical Standards, using a ranking, descriptive, paired comparison, sensory profile testing and scaling methods. For the purpose of statistical evaluation, Friedman, Kruskal-Wallis, PCA and Faktor analysis were used. Level of abilities and sensory experience of panelists were found comparable to consumer testing. The results include sensory characteristics of all of the beverages tested. In general, the one-component beverages were preferred to the two-component beverages and the additive colouring with aronia concentrate wasn´t found any benefitial, from the sensory point of view. The consumer questionnaire proved all of the samples to be very promising, but based on the sensory results, we would consider the mint and the sage-mint beverages being the most potentialy applicable samples.
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Aroma active compounds of selected types of herbs
Škutová, Pavla ; Hrstka, Miroslav (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with the identification of aroma active compounds in medicinal herbs. The group consists of 5 types of herbs: lemon balm (Melissa officinalis), pot marigold (Calendula officinalis), clary sage (Salvia sclarea), milk thistle (Silybum marianum), goat’s rue (Galega officinalis). These plants are known for their positive effects in folk medicine. The SPME-GC-MS method was chosen to characterize the fragrances. During the identification of volatile compounds, emphasis was put on suspected fragrance allergens listed in EC Regulation 1223/2009 in Annex III. Overall 106 compounds were identified together with 8 allergens in lemon balm, pot marigold contained 104 compounds with 7 allergens included, 82 compounds including 5 allergens in clary sage, 73 compounds were identified in milk thistle including 6 allergens and 110 compounds with 9 allergens included in goat’s rue.
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Suburban park Bažantnice (Kladno) in Botany and Dendrology Instruction
Veverka, Vojtěch ; Novotný, Petr (advisor) ; Hrouda, Lubomír (referee)
The topic of this thesis is forest park's Bažantnice mapping. The thesis suggests the way of aplication of botanic terrain education. Botany and dendrology is one of the main educations question on elementary and secondary school. Park Bažantnice can be used in science lesson for its hight potential. The thesis i s divided into two parts. The first of them summarizes differents types of the terrain education and focuses on didactic using of the park Bažantnice. The second one deals with the station mapping and describes way of data colecting and adapting. Accompanying pictures and generated maps are included in appendiced at the back of the thesis. Keywords: dendrology, biology, botany, education, park, Bažantnice, Kladno, mapping, herbs, woody plants, terrain education
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The influence of animal fertilisation and application of ferment on plant species composition of permanent grasslands
LHOTSKÝ, Jan
The presented thesis is focused on the drohové representation of grasses, herbs and weeds on permanent grassland fertilized animál fertilizers and digest with biogas stations. The work is based on intensive observation period from 1. 11. 2014 30. 10. 2015. In the following pages you can read on the use of forage crops in agriculture, about the importance of energy, about the importance of grassland in the landscape, ecology and tourism ventures. Furthermore, the work deals with the care of permanent grass, their formation, choice of suitable forage species in these locations and the choice of species by the use of sward. No small part is gocused on Biogass station, on source substrátes and digest output and its use. The main part of the resolves and deals with the correct handling of the fertilizer, their descriptions, and the importance of the species. There is also a reference concerning the legislation and when it is possible use of the fertilizer, which can be applied, regulas conservation etc. This section describes the results of my monitor three different fertilized grass stands permanently in similar or the same climatic conditions. We can find here describes the variation in account growth, composition, density, yield, guality and fitness for a particular purpose. Unfortunately, there is also a negative effect of weather on described vegetation in the summer of 2015, when we hit the tropical temperatures and drought. For this reason, my observations of very different from other studies, especially in the summer and Autumn period.
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Herbal alternative treatment for various diseases
MICHALÍK, Petr
The theme of the thesis is Herbal alternative treatment for various diseases. Bachelor thesis is focused on medicinal plants used by the human population since time immemorial, which are used to this day, not only for treatment, but also for prevention. The introduction mentioned general part, then the history of herbal medicine, construction plant, positive and negative effects of the herbs, contained substances of herbs, harvesting, drying and finally mentioned individual herbs. The aim of this study was to map the use of herbal alternative treatments to the public within the primary and secondary prevention. The basis for the continuing part happened questionnaire regarding age population from 18 years, the results of which revealed some interesting information about the public awareness of this method of treatment using medicinal plants. The results also showed a preference for herbal or conventional treatment, then what are the most practiced methods of natural medicine and the most popular form of their use. The results also show that respondents still consider herbal therapy as an important complement to conventional medicine. Based on these findings, conclusions were drawn and set out recommendations for practice.
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Aroma active compounds of selected types of medical herbs
Pecinová, Ester ; Diviš, Pavel (referee) ; Vítová, Eva (advisor)
This thesis deals with aroma active compounds of selected types of medicinal herbs, specifically lavender (Lavandula officinalis), peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and hyssop (Hyssopus officinalis). In the theoretical part the single herbs were characterized including botanical description, chemical composition, medicinal properties, adverse effects and the practical use; aroma active compounds present in herbs are also emphasized. Based on the literature search, gas chromatography coupled with mass spectrometry as currently the most frequently used method, was chosen for the assessment of aroma compounds. Solid Phase Microextraction was used for isolation of volatile compounds from dried herbs. Within experimental part of the work selected parameters of the SPME-GC-MS method were optimized, aroma compounds in samples of herbs were identified, and twenty four suspected fragrance allergens due to the Regulation (EC) N° 1223/2009 were also identified and quantified. Overall 69 compounds were indetified together with 2 allergens in lavender, 59 compounds including 5 allergens in peppermint, sage contained 49 compounds with 4 allergens included, 41 compounds were identified in St. John’s wort including 4 allergens and finally 64 compounds with 4 allergens included in hyssop.
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