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Cardioprotective effect of adaptations to hypoxia and cold: Connexin 43 and hexokinase 2
Kohútová, Jana
Ventricular arrhythmias are the main cause of death worldwide. An increased incidence of arrhythmias in the heart of mammals is accompanied by a remodeling of the cellular distribution gap between the channels of mainly connexin 43 (Cx43). Recently has been demonstrated significant effect of mitochondria and their association with arrhythmogenesis. Various pathological conditions alter the expression and / or distribution of Cx43, depending on the phosphorylation status. but also on altering the association of hexokinase with mitochondria, which reduces the likelihood of apoptosis activation. Adaptation to intermittent hypobaric hypoxia potentiates endogenous pathways reducing the incidence of ventricular arteries, whereas continuous normobaric hypoxia does not have this effect. Another studied model is cold acclimatization, which has been known for several decades by known effects on human health. However, the heart study of these models in relation to Cx43 is missing. Our goal was to determine the expression of Cx43, phosphorylated Cx43 (p-Cx43) and hexokinase (HK1, HK2) and their distribution in cardiomyocytes. In addition, the expression of Cx43 upstream kinases, protein kinase A, protein kinase G, casein kinase 1 in normoxic and hypoxic left ventricles of rats, along with Cx43 distribution...
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Cardioprotective effect of adaptations to hypoxia and cold: Connexin 43 and hexokinase 2
Kohútová, Jana ; Žurmanová, Jitka (advisor) ; Radošinská, Jana (referee) ; Barančík, Miroslav (referee)
Ventricular arrhythmias are the main cause of death worldwide. An increased incidence of arrhythmias in the heart of mammals is accompanied by a remodeling of the cellular distribution gap between the channels of mainly connexin 43 (Cx43). Recently has been demonstrated significant effect of mitochondria and their association with arrhythmogenesis. Various pathological conditions alter the expression and / or distribution of Cx43, depending on the phosphorylation status. but also on altering the association of hexokinase with mitochondria, which reduces the likelihood of apoptosis activation. Adaptation to intermittent hypobaric hypoxia potentiates endogenous pathways reducing the incidence of ventricular arteries, whereas continuous normobaric hypoxia does not have this effect. Another studied model is cold acclimatization, which has been known for several decades by known effects on human health. However, the heart study of these models in relation to Cx43 is missing. Our goal was to determine the expression of Cx43, phosphorylated Cx43 (p-Cx43) and hexokinase (HK1, HK2) and their distribution in cardiomyocytes. In addition, the expression of Cx43 upstream kinases, protein kinase A, protein kinase G, casein kinase 1 in normoxic and hypoxic left ventricles of rats, along with Cx43 distribution...
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The effect of acute cold and permanent light to left ventricular of the rat heart
Vítková, Ivana ; Žurmanová, Jitka (advisor) ; Bendová, Zdeňka (referee)
Acute cold exposure increases the risk of sudden cardiac events, similarly exposure to constant light negatively affects the cardiovascular system. However, the individual effects of these factors and the effect of their combination on cardiomyocytes are not yet known. The thesis deals with the influence of a 3 day cold exposure and constant light on the expression of β-adrenergic receptors and associated G-proteins in association with apoptotic signals in the left ventricle of the Wistar rat heart. In this work apoptotic proteins BAX, BCL2, caspase 8 and important components of β-adrenergic signalization - β1, β2, G-proteins, Gas, Gi1/2 and Gi3 were determined. The relative expression of the proteins was analyzed by Western blotting. The results confirm the detrimental effect of cold and light exposure. However, the synergistic effect of these two stressors shows surprising results.
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Increasing the energy efficiency of industrial heating plant
Spišák, Lukáš ; Lisý, Martin (referee) ; Kracík, Petr (advisor)
This bachelor thesis consists of two parts. The introductory chapters are devoted to the basic description of cogeneration and trigeneration technologies, focusing on the description of cogeneration units and cooling devices. The second part of the thesis compares two specific ways of using cogeneration units and gas boilers in the CHEMOSVIT ENERGOCHEM a.s. in the town Svit. Subsequently, both variants are evaluated energetically and economically.
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Physiological mechanisms of abiotic stress tolerance in Sorghum bicolor
Kratochvíl, Jan ; Konrádová, Hana (advisor) ; Lhotáková, Zuzana (referee)
Current agriculture is facing a serious challenge of decreasing precipitation and irregular occurrence of drought periods including their unfavorable distribution during the vegetation season. This leads to growing interest in planting highly drought-resistant crops like sorghum. In comparison with other crops, sorghum excels in low water demand, though exhibits high susceptibility to low temperatures, which hampers its spread to new regions. Surprisingly, there is not enough information about the nature of sorghum's reaction to cold exposure. The aim of this diploma thesis was to describe reactions of young sorghum plants exposed to cold stress, low water availability and their combination and to verify the possibility of plant hardening through previous low-stress load. The special focus was paid to changes in carbohydrate metabolism, which plays generally very important role in plant defense reactions. The other analyzed physiological traits were leaf tissue osmotic potential, proline content and basic morphometric characteristics. Experimental design consisted of pot experiments conducted in growth chambers and the experiments performed under controlled conditions in vitro, using two sorghum genotypes "Ruzrok" and "01Z1800012". Both genotypes exhibited similar response to stress treatment....
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Efficiency of cold treatment of locomotive system dysfunctions
Vášková, Mariana ; Malá, Jitka (advisor) ; Vláčilová, Ivana (referee)
Title: Efficiency of cold treatment of locomotive system dysfunctions Objectives: The theoretical part should provide an overview of effects of cold on the human organism. The practical part reports a pilot study in which local cryotherapy was applied to painful areas of dysfunctional locomotive system. Methods: After measuring local painfulness using an algometer, the Cryogen 2 device was used for local cryotherapeutic treatment; after the treatment, algometer was used again. Subjective state of the patient was monitored throughout the process. Cryotherapy was applied within normal individual physiotherapeutic treatment to patients of varying age, sex, and locomotive system dysfunctions. Results: We have shown that local cryotherapy, using the Cryogen 2 device, did decrease painfulness in measured areas. Although significant differences in pain perception were found between male and female patients, the efficiency of cryotherapy did not depend on sex. Some correlation was found between treatment efficiency and age. No evidence was found for establishing that repeated application decreases pain perception measured before the next treatment. The results are influenced by a smaller sample of patients and measurements, and by inhomogeneity of patients. Key words: pain treatment, cold, local cryotherapy,...
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Therapeutic and Regenerative Effects of Cryotherapy on the 50+ generation
SOSNOVÁ, Eva
The theoretical part of the thesis deals with the life style, regeneration, and relaxation with the focus on the application of cold to treat the elderly. The work explains the use of cryotherapy from both historical and scientific points of view. The aim is to introduce the method of cryotherapy as a popular method which postpones the beginning of dependency and motion impairment with people aged over 50 and thus enables them to keep active much longer. Although the 21st century medicine has reached an extraordinary success, the number of illnesses does not decrease. There is a wide variety of civilisation illnesses resulting from the wrong life style. For such illnesses drugs with frequent side effects are prescribed. The work introduces cryotherapy as one of the methods which can serve the health protection, prevention, the treatment of traumatic injuries, and the supportive therapy of different health problems with relatively no side effects.
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A source of cold hydrogen atoms
Podolník, Aleš ; Plašil, Radek (advisor) ; Kaňka, Adolf (referee)
Vodík v molekulární a atomární formě je nejčastější prvek ve vesmíru a proto jsou chemické reakce, ve kterých vystupuje, důležité pro pochopení nejen formace prvních hvězd a galaxií, ale i pro studium současných dějů v mezihvězdném plynu. Studium například reakce H- + H -> H2 + e- (asociativní odtržení) ujasňuje náhled na proces tvorby molekul vodíku a umožňuje upřesnění kvantově-mechanických modelů. Pro tento a další experimenty je na KFPP přítomen zdroj chladného atomárního vodíku. V práci je popsána aparatura zdroje a její operace, navrhováno a částečně realizováno zlepšení uspořádání vnitřní konstrukce a jsou proměřeny základní charakteristiky zdroje.
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Temperature as Source Sphere of Conceptual Metaphors
Hýsková, Tereza ; Vaňková, Irena (advisor) ; Pacovská, Jasňa (referee)
This thesis is focused on a research of the source domain of temperature and related concepts. It is based on the approaches of American and Polish Cognitive Linguistics. The first part is regarded to the relationship between the concepts of WARMTH / COLD and other basic concepts. The crucial part of the thesis deals with temperature as the source domain in conceptualization of emotions, emotional states and human behaviour. This part includes the description of general phenomenon in the process of mapping from the source domain of temperature to the target domain of emotions. The next chapters are focused on the Czech expressions related to separate emotions which are viewed as the target domains. There are marked the source domains of WARMTH, COLD, HEAT and FIRE in the thesis. These source domains are considered to have different roles and the human corporealness is assumed to be the main reason for the differences.
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Power - plant
Studený, Jan ; Severa, Zdeněk (referee) ; Mléčka, Jan (advisor)
The "POWER - PLANT" deals with the rehabilitation of the former Central Power Conversion and Dale Schoeller (Nejedly I and III) in the village Libušín Mine. Will be newly designed for building the power plant technology with progressive fluidized bed boiler to biomass steam turbine with an output of 7 MW and absorption (trigeneration) unit producing heat, cooling and electricity, which also will function as a cooler primary circuits. The source of water for the power plant will not only former mining pit Nejedlý I which is currently flooded drinking water at about 12 ° C. This system uses high efficiency embedded fuel (biomass), which is ultimately required less. A greater proportion of electricity generated and part of the heat will be distributed to the public network. In most areas will be proposed publicly accessible greenhouses of steel-aluminum construction filled ETFE foil forming the heat insulating membrane filled with air. The reason is to create conditions for the cultivation of tropical and subtropical plants. Greenhouses will be connected to the absorption unit power (underground meanders through which water will circulate), the ventilation shaft mine and mine water - therefore it will be possible to manage the conditions of the internal environment of the building without the influence of the season and especially without mounting other technological devices. It will provide for cooperation with the absorption unit for cooling the primary circuit - therefore eliminating the need for cooling towers or fans. The project will build a detached departments of the Faculty of Mechanical Engineering in Prague - Institute of progressive technologies and systems for energy and the Faculty of Agronomy and Natural Resources CULS. The reason is to allow students and scientists actively and in practice mainly participate in the operation and especially the development of the issue. The public in the area besides themselves open to the greenhouses indoor and outdoor thermal swimming. Part of the entrance hall is a bistro and foremost lecture hall. The aim is decentralized botanical-energy complex of buildings that produce electricity, heat, cooling, and biomass, and creating recreational, educational and research conditions.
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