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Influence of supplementation with n-3 polyunsaturated fatty acids on inflammatory markers in patients on long-term parenteral nutrition
Svěchová, Hana ; Novák, František (advisor) ; Meisnerová, Eva (referee)
SMOFLipid® is a commonly used fat emulsion for parenteral nutrition. We investigated how enrichment of SMOFLipid® with n-3 polyunsaturated fatty acids (PUFA) in a form of second fat emulsion, Omegaven® , changes fatty acid composition of total plasma phospholipids and erythrocyte phospholipids, cytokine concentrations in serum and in supernatant from in vitro whole blood culture stimulated with lipopolasaccharide (LPS) and we evaluated also changes in oxido- reductive balance. Eight patients on long-term home parenteral nutrition recieved both emulsions, SMOFLipid® (6 weeks) and SMOFLipid® +Omegaven® (4 weeks), one by one. We observed no significant differences in common laboratory and clinical parameters between these two types of diet. Enrichment of SMOFLipid® with Omegaven® led to an increase in eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) in total plasma phospholipids and there was also an increse in proportion of EPA in erythrocyte phospholipids, while proportion of DHA remained unchanged. These changes were in both phospholipids of plasma and erythrocyte compensated for a decrease in proportion of linoleic and arachidonic acid (n-6 PUFA). There were elevated IL-6 and TNF-α serum concentrations in patients after both diets. There was a decrease in IL-6 production by 36% with SMOFLipid®...
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Optimization of measurements by the HPGe detector with a very low background for achievement of the low MDA values
ČERNÁ, Kristýna
Abstract My thesis examines the measurement optimalization when the semiconductor HPGe detectors with a low background and a high relative efficiency are used for achieving low values of MDA (minimum detectable activity). I conducted my research in the Department of Spectrometry of SÚRO (National Radiation Protection Institute) in Prague. In this laboratory the standard geometries of detectors with lower efficiency are used. The aim of this thesis is to offer a proposal of new geometry, sizes and shapes of measurement cells which will better correspond with the demand for higher efficiency of updated detectors. The assignment was to find the best mathematical function to descibe the correlation between efficiency and the direction of the radiation point source towards the detector. This function is derived from experimentally obtained data. To conduct measurements, three kinds of detectors with high relative efficiency used for activity measurements at different geometries were selected. As the source of gamma radiation, the point sources with energies of 46 keV, 661 keV, 1332 keV in different distances and positions were used. The function was applied separatelly to each of the three detectors and to the areas above the detector and sidewards of the detector. The objective of the research was to project new measurement geometries or to optimalize the current ones which could be used also in other detectors with high efficiency. After integrating the function in the cylindrical area above the detector and the annular area sidewards of the detector, areas contributing substantially to the overall efficiency were determined. These areas may be considered to be the optimal sizes for new geometries in given positions. After combining all the optimal areas above the detector, sidewards of the detector and for selected energies and the detector, optimal sizes of a new geometry were found. The function can be used both in the front part and the side part of the detector but its validity is limited. The value of MDA for the particular geometry is in indirect proportion to the efficiency square root and thus this new geometry should lead to minimum values of MDA.
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Consumer preferences' based testing of consumer products
SOCHEROVÁ, Klára
I determined the preferences of mobile phone attributes on the basis of an on-line consumer research, which I conducted with a questionaire created in the Google Docs application. Thanks to the questionaire, I was able to gain information about the evaluation of the importance of 19 functional parametres made by the queired respondents. Through the use of this evaluation I counted the strength for particular parametres and various segments of customers. Differences in the importance of atributes for selected segments were found especially among the categories of age and sex. The teenage generation prefers Bluetooth or a high capacity of the mobile phone memory also in means of memory stics. Enquired young men attach the highest importance to the same attributes as teenagers, but for women in the same category is crucial particularly the visual image of the mobile phone, such as the size of the display, its proportions and weight. What did not have any success among the particular parametres, was the function of TV, which was put on the last place on the imaginary ladder of importance. I stated the recommended telephones on the basis of calculated stregths of the attributes, point evaluation of the experts and price of the selected mobile phones. Among the five recommended products I would like to mention telephone brands {\clq}qNOKIA 6300``, {\clq}qSONY ERICSSON W910i``, {\clq}qNOKIA 3110``, {\clq}qSONY ERICSSON W760i`` a {\clq}qSAMSUNG SGH i8510``.
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Comparison of methods of ECLIA and ELISA in determining the beta-CrossLaps
KOŽANTOVÁ, Jana
Osteoporosis is a very old disease affecting a large majority of population. Its occurrence rate has been growing permanently. It is a systemic disease of bones for which the decrease in bone mass, i.e. of inorganic as well as organic bone part is characteristic. Typical symptoms are osteoporotic fractures. To perform an in time diagnostics of the disease, it is necessary to execute the necessary examinations. One of them is also the laboratory determination of markers of bone resorption and new creation for finding out the state of bone metabolism. The target of my bachelor thesis was to find out the level of the bone resorption marker (beta-CrossLaps) by means of ELISA and ECLIA methods on the same serum from patients with the diagnosed osteoporosis and to process statistically the results. In the next part I dealt with the structure and functions of bones. Then I focused on markers of bone formation and resorption. Finally I mentioned the bone metabolism and its control mechanisms. I concentrated mainly on the metabolism of calcium, D vitamin, parathormone and calcitonin. I carried out the practical part of my bachelor's thesis in the accredited private laboratory STAFILA, spol. s r. o. in České Budějovice. The procedure of my work followed the standard operating procedure of the laboratory. In the research, in total 66 serums of patients with osteoporosis were analysed by me. At first, I determined the concentration by beta-CrossLaps method based on the principle of sandwich ELISA on the appliance NexGen Four from TestLine company. Then I analysed the same samples on the automatic analyser IDS-iSYS from IDS company, this time by ECLIA method. Both appliances on which the determination was executed, principles of both methods, applied analytic sets and the procedures of analysis are described in the methodical part. In the next part of my bachelor's thesis, the results of my measuring are stated. At first I entered the concentrations beta-CrossLaps in ng/ml measured by both methods into the table and for the respective samples I stated the sex and years of birth of individual patients. Moreover I prepared a graph illustrating the share of men and women of various age categories in my research. The second graph shows the differences in values of beta-CrossLaps concentrations between both methods. Resulting concentrations were subject to regression analysis. I applied linear regression and Grubbs test for finding out the outlying values excluded from the further statistical processing. I performed Passing-Bablok regression based on the remaining values. As a result of regression equation and 95% CI of the slope B and intercept A, the proportional error of measuring was rejected but on the contrary, the systematic error was confirmed. Correlation coefficient R = 0,97 shows a very good linear dependency between both methods. It turned out by practice in the laboratory that the evaluation of concentrations beta-CrossLaps from the same patients on various appliances may show partially different values. I worked at two different analysers, each of them was based on another principle, with a different sensitivity and calibration. All of this could have the influence on the resulting concentration of individual samples. The advantage of automatic analyser IDS-iSYS is a quicker determination. Moreover it is possible to perform on this apparatus all the possible examination concerning the bone metabolism from D vitamin, parathormone, osteocalcin up to bone markers. It is advantage for the patients with osteoporosis as well as with other metabolic skeleton diseases. It follows from this that all the necessary examinations may be carried out on one apparatus.
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Detection of body fat percentage by using the caliperation on Viva Fitnes clients in České Budějovice
PELEŠKOVÁ, Anna
The aim of this Bachelor Thesis is to detect a body fat percentage among clients attending fitness centre Viva Fitnes in České Budějovice. In total, two hundred respondents of both sex were measured and analysed. The measuring was mainly concerned with gym and indoor lessons clients. Basic somatic proportions and body fat were surveyed via skin fold Pařízková caliperation method. The procedure and fieldwork results are listed in the practical part. The theoretical part provides current knowledge overview regarding the issue of human body composition necessary to understand the basic issues of the Thesis. The resulting statistic values are processed in form of graphs, charts and statistical significance.
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Fractural based microstructural damane evaluation in Ni-based superalloy gas turbine blades
Tarafder, M. ; Dlouhý, Ivo ; Hore, S. ; Das, S. K.
A fractal based evaluation of microstructural damage in Ni based superalloy cast IN 738 LC used in gas turbine blades is presented in the paper. Non-destructive in-situ replica method was used without damaging the blades for the evaluation of microstructure. The theoretical life of the stator blades of the gas turbine, which was exposed to temperature varying between 1060°C to 1100°C, was approximately 100,000 hours. In order to assess the suitability of the stator blades after 50% of the service life, fractal based microstructural damage evaluation has been conducted. The fractal dimensions of the in situ metallographic images can be correlated to the progressive deterioration of hardness at various locations of the blades. The extent of damage is inversely proportional to the fractal dimension. For fractal dimension below 2.4, the material can be classified as substantially damaged.
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Some New Approaches to the Characterization of the Carbon Paste Electrodes
Mikysek, T. ; Švancara, I. ; Ludvík, Jiří ; Vytřas, K. ; Kalcher, K.
Uhlíkové pastové elektrody mají dvě hlavní výhody: jejich povrch lze snadno reprodukovatelně obnovovat a dále, je možné je modifikovat v objemu za účelem získání specifických senzorů. Příprava těchto elektrod však závisí jednak na použitých materiálech, tedy na uhlíkovém prášku i na olejovém médiu, jednak na jejich vzájemném poměru. To umožňuje velikou variabilitu vlastností, současně ale otázka charakterizace těchto elektrod je velmi významná. Bylo zjištěno, že graf závislosti odporu elektrody na jejím složení je ve tvaru dvou protínajících se přímek. K vysvětlení této závislosti byl navržen, testován a diskutován model vycházející z představy těsně uspořádaných koulí (známé z krystalografie). Další způsob charakterizace těchto elektrod je založen na interpretaci cyklických voltamogramů nějakého dobře reverzibilního systému. Obě tyto metody jsou kompatibilní, navíc umožňují vysvětlení dalších jevů spojených s morfologií a se stárnutím těchto elektrod.
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Influence of the elections and electoral systems on dissolution of the Socialist federal republic of Yugoslavia
Höfer, Karel ; Buben, Radek (advisor) ; Koubek, Jiří (referee)
The author is concerned with the influence of elections and electoral systems on dissolution of the Socialist Federal Republic of Yugoslavia at example of Slovenia and Croatia in his contribution from the field of political science. He analyses the role of elections and electroral systems in the republic parliaments of Slovenia and Croatia on the basis of methodological procedures and theoretical concepts. It is done primarily by the transformation of allocation of political power before and after the elections and also by the consequences of the elections. Together with it the reader is briefed by the basic facts about dissolution of Yugoslavia and emergence of selected successor countries. The infuence of elections at the level of republics consisted in the legitimization of transformation of allocation of political power toward to the republics and in the legitimization of democratic way above all. The influence of electoral systems consisted in proportion of the results above all. The elections at the level of republics simplified the decentralization of Yugoslavia leading to its dissolution. Elections and electoral systems was significant part of the dissolution of Yugoslavia and the independence of its parts. The desitegration process was far more complex and its pure reduction for the election would...
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Burnout by Nurses in Intensive Care
Jánová, Simona ; Mellanová, Alena (advisor) ; Honzák, Radkin (referee)
Burnout by nurses in intensive care Abstract: Objective of this thesis was to determine incidence of the risk of burnout by nurses in intensive care in the Motol University Hospital in Prague, compare to risk of burnout among nurses in intensive care for childern and adults and by age, sex, marital status, education, length of experience in health care and on the current department, satisfaction and performance in the workplace. The research method for data collection were two anonymous questionnaires: one prepared by author of the thesis and a standardized questionnaire by dr. Pines to determine the risk of burnout. It was found that more than hlaf of nurses in FN Motol had low risk of burnout and has not demonstrated that nurses have on children's or adult wards increased risk. Results confirmed that discontent and the increasing intensity of stress in the workplace is proportional to the risk of burnout. This research confirmed that although most nurses achieved good results has been shown a high percentage of nurses with alarmig score and even burnout. Therefore we should not ignore this issure, but rather to warn him and avoid unpleasant consequences through educational seminars, pleasant working environment and do not overload nurses. Keywords: burn-out, stress, nurses, intensive care, mental hygiene
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Biodiversity of standing floodplain waters and processes of its formation and preservation
Pithart, David
Water chemistry and planktic communities in the naturally eutrophic alluvial waters in the Lužnice floodplain have been studied. The floofplain waters are naturally eutrophic, with strong vertical physico-chemical gradients and oxygen depletions at the botom. Small pools and backwaters of this type have unique phytoplankton structure. Prevailing dominance of flegellates, namely Cryptophyceae, and low proportion of green chlorococcal algae and Diatoms, and almost absence of Cyanophyceae are very unusual for such eutrophic waters. Flagellate dominate thanks their ability to keep the vertical position in a permanently light limited conditions (resulted from poorly mixed water columns and intensive shading of water surface by vegetation). Floodplain geodiversity play crucial role, determining morphology and location of pools and backwaters, their exposition to flooding and inflows of underground water. Terrestial vegetation effects light exposition, leaf litter.
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