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Age estimation based on human dentice
Černá, Magdaléna ; Velemínská, Jana (advisor) ; Brůžek, Jaroslav (referee)
Age estimation plays a very important role not only in forensic and biomedicinal anthropology, but also in other disciplines, such as bioarcheologie or paleoanthropology. Teeth are considered the most reliable age indicators during childhood and adolescence. Teeth are resistant to the effects of external and internal factors, and because of that they decay a long time after a death of an individual. While age estimation in children and adolescents can be based on mineralization or eruption of the teeth, in adults it is focused on retrogressive changes in dentition. There have been established several methods and their modification for age assessment based on these criteria. This thesis aims to generally summarize tthe principles and methods of age estimation based on dentition both in immature and adult individuals.
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Utilization of forensic dentistry in indentification of individuals
Fialková, Martina ; Velemínská, Jana (advisor) ; Stránská, Petra (referee)
Forensic identification and age estimation has a significant role in cases when the unknown deceased body is found, after mass disasters when it is necessary to distinguish victims, but also in guestion of imigrants. And just these areas are very important part of forensic odontology, because dental development like a complex proces takes place from early foetal life to approximately 20 years of age is less affected by endocrine diseases or nutritional variations than other tissues. Dental age estimate is fundamental mainly in cases of children and young people, which teeth are still growing and they are in different developmental stages. On basis of these stages is possible to obtain very accurate results.
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Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
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Determination of toxic elements in wines
Hajdučková, Iva ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
The aim of this study was to determine the occurrence of selected toxic elements in wine. The theoretical part described technology of wine production and selected toxic elements (cadmium, lead, copper, zinc, nickel and chromium) in the terms of their impact on wine quality and their effects on the human organism. It provides an overview of instrumental analytical methods, which can be used for the analysis of toxic metals. In the experimental part inductively coupled mass spektrometry technique was used for the analysis of wine samples. The measured values are statistically processed and compared with current legislation.
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Selected inorganic pollutants in foodstuffs
Kroupová, Kateřina ; Vítová, Eva (referee) ; Vitoulová, Eva (advisor)
This bachelor thesis is focus on occurrence of inorganic pollutants in foodstuffs. The introduction is dedicated to apperance of individual selected elements in foodstuffs. Concentration of these elements is very important factor for examination of toxic effects for the human organism. If we want to avoid the harmful effects to the human organism, we have to define border limits. Work is based on the legislative of Czech republic. Second part of this study is focused on the biological monitoring. The biological monitoring is important for precautionary measures. In the third part of this study are described toxical effects of these elements to the human organism. Before qualitative and quantitative analysis by instrumental techniques is important to properly take the sample. In the fourth and fifth part of this work are mentioned methods used for determination of these elements and metods of taking samples. This bachelor thesis provide complex view on the problems of pollutants in foodstuffs and it should be used as a base for following diploma thesis.
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Microstructure of a tribosphenic molar - comparative analysis
ŠPOUTIL, František
The proposed thesis concerns in the study of tribosphenic molar, the key apomorphy of mammalian clade, mainly in structure and development of its enamel coat. As the main model species served us European vespertilionid bat Myotis myotis. The aims of this thesis are: (1) to describe structure and microstructure of enamel in tribosphenic molars in detail; (2) to compare it with unicuspid teeth of the same dentition; (3) to describe mineralization process and enamel maturation in insectivorous dentition; (4) to describe late odontogenesis in molars of insectivorous dentition and its interlacing with enamel maturation, and how they come to occlusion.
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The proportion of minerals in the soil of selected layers in the region of Stropnicko
KOTOUSOVÁ, Zdeňka
My work targets the change in content of the organic soil karbon and basic cations in relation to changes of area usage and agricultural management of the chosen special {--} interest places of Stropnicko. In the monitored period from 2001 till 2007, the soil organic mass markedly fell off. The greatest decline was in the forest ecosystem in all researched basins. Decline of organic soil karbon content was also found on permanent grass growth, but it was much less than in the forests. On the contrary mild improvement turns out on the arable land. Next pH value of soil leach was monitored. The greatest change was shown in the forests. The greatest increase of PH value was at the area of Pasecký potok. In other basins, expected Veverský potok, pH value increased too. On the contrary, there is decline of pH value on the permanent grass growth and arable land . There were not statistically great advancements between 2001 and 2007 in the soil mineral reserve, especially of the basic soil cations. The significant decline of sodium is the exception. I have suggested steps that lead to the steady way of farming in the agricultural foothills. I have recommended to increase supplied barnyard manure into arable land, the optimalization of cow units for pasture area and the gradation of the forest communities to deciduous, eventually mixed stands, according to the vegetation levels.
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