National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
Adhesion, growth and stress adaptation of vascular smooth muscle cells in cultures on collagen I degraded by matrix metalloproteinases-13
Bačáková, Lucie ; Herget, J. ; Novotná, J. ; Eckhardt, Adam ; Lisá, Věra
Degradation of collagen I by matrix metalloproteinase-13 resulted in a lower adhesion, higher proliferation, higher concentration of heath shock protein 60, higher cell death and loss of differentiation markers in rat aortic smooth muscle cells in cultures on this substrate.
Ultrastructure of nuclear RNP particles extruded into cytoplasm in apoptotic cells
Krajčí, D. ; Mareš, Vladislav ; Lisá, Věra
In C6 glioma cells undergoing cisplatin induced apoptosis,structures resembling degraded nucleoli were frequently observedin the perinuclear cytoplasm. In some cells, these originallynuclear structures mooved through deep invaginations of the nuclearenvelope, while in the other cells breakdown of small nuclearlobules is the source of this cytoplasmic component.

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